pycom02g25040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014785
Physical Location & Seq
Reverse (-)
23071331 .. 23073326
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25040.1

Sequence Viewer

Length: 1161 bp
ATGGGCAAGTGGATTCATCGTCCCCGCCGTAGCTTCTGCCGTCAGGAAAAGCTTCCGGACCCATCGCCTCTGCCATTTCACCCTCAACCTCCTCCTCCTTCTGGGAGTCGGCAGGATGGTATACCTTATTGGGAAAAGCAGTACTGCACGTTGGTTGGGTCTATACCATGGTGGAAGGTAGTAGATGCAAAGAATTATATGTATGGAAGCAGTATTATACTCAATTGGAATGACTCAGGTGCAGAGGAGGCATTTCAAAATGCAAAAAAGCGCTTTTGGGCAGACATCAATGGTCTCCCCTTTGACGCATCTCTGCCTGATCCAAATATTTATATTGATGCAATAGATTGGAACCCTAACATCGATCCTGCACTCATTAAGGAAGTGGATCGAGAGTACTTTGCTCCTGATGAGGGAGAAGGGCAAGGCAAGATTGAGCAAACAAACAAGAGGGCAAAATATTCAGATTTTGTTCCTCCCGATGAACATAACACTGTCCCAGATACTCATACAAATTCTTGGGAATGTGACAACATTCAGGGTAATGGACATTTGATAAATAAAGCTCCGGGCAGGAACCAGTGGAAAGCTAATCGTGACGACCCTTGGGAATGTGGAATTACTCAGGGCAATGGAACTACTGAGACGGAGAAGAATGCATGGGAAGATAGAAGGAATGAGTCGCGGGGATGGAACCAGATGGAGGACGGTATCACCGCGCCAAAGAGTTGGGATAATGGTGTCAATCCTTGGGAAGGAGGCTGTCAGGATGTTGCCTCTGTGAAAGGTAGTAGATGGGCTGATCCGGTGTCTAATTCTTGGGGCTGCGACCAGGAGTCAAAGAACTTGGGTCGTGGTCAAAATTCTTGGAAGTATGGTCCTAGTCAAAACAGCAAATCTCTAAAAGATAGAGAATGGAGAGATCGTAGAGGAGATGAATGGGGTCAGAAACAGTGGGAGAGCCATAATAACCAGAAAAATAATTCGGATTTTGGGATAACAAATAGTGGTCGGGGAGCAAGGAACGATGGTGGCTATAAGAGGGGACACCCTCACCAGTATAGATCAGGCTATAAAGGCTCAAGATTGCAAGGGAGTGATTATCAAACAGGTAACTACTGGAGTAGGGAAAATAACAGAAAGAGGGTCAGTTTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

387

Amino Acids

44.26

Weight (kDa)

6.73

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014785)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02130 FvH4_7g02130
malus_domestica MD02G1297200.v1.1
prunus_persica Prupe.2G026900_v2.0.a1
pyrus_communis pycom02g25040 pycom07g02040
rosa_chinensis RchiOBHm_Chr1g0319371
rosa_laevigata RLG00000030492
rosa_multiflora Rmu_sc0005870.1_g000004 Rmu_sc0014506.1_g000002
rosa_roxburghii Rroxscaffold_4G00328530
rosa_rugosa Rorug01G0027200
rosa_samantha Rh1AG039400 Rh1BG036200 Rh1CG038300 Rh1DG031500
rosa_wichuraiana Rw1G003150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 121
AccII CGCG 2 cut(s) 685, 719
AccIII TCCGGA 1 cut(s) 55
AciI CCGC 3 cut(s) 25, 685, 717
AclWI GGATC 4 cut(s) 314, 359, 396, 797
AcsI RAATTY 2 cut(s) 514, 862
AfaI GTAC 2 cut(s) 143, 398
AfeI AGCGCT 1 cut(s) 272
AfiI CCNNNNNNNGG 4 cut(s) 101, 413, 703, 755
AgsI TTSAA 1 cut(s) 257
AhdI GACNNNNNGTC 1 cut(s) 835
AjnI CCWGG 1 cut(s) 831
AluBI AGCT 4 cut(s) 33, 52, 566, 590
AluI AGCT 4 cut(s) 33, 52, 566, 590
Alw26I GTCTC 2 cut(s) 299, 638
AlwI GGATC 4 cut(s) 314, 359, 396, 797
Aor13HI TCCGGA 1 cut(s) 55
Aor51HI AGCGCT 1 cut(s) 272
ApeKI GCWGC 1 cut(s) 825
ApoI RAATTY 2 cut(s) 514, 862
Asp700I GAANNNNTTC 1 cut(s) 51
AspLEI GCGC 2 cut(s) 273, 721
AspS9I GGNCC 2 cut(s) 58, 878
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 3 cut(s) 71, 706, 1046
AvaII GGWCC 2 cut(s) 58, 878
BbvI GCAGC 1 cut(s) 812
BccI CCATC 6 cut(s) 70, 110, 684, 694, 789, 1022
BceAI ACGGC 2 cut(s) 12, 24
BciT130I CCWGG 1 cut(s) 833
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 299, 638
BfaI CTAG 1 cut(s) 882
BfoI RGCGCY 1 cut(s) 274
BisI GCNGC 1 cut(s) 826
BlsI GCNGC 1 cut(s) 827
BmcAI AGTACT 2 cut(s) 143, 398
Bme1390I CCNGG 2 cut(s) 570, 833
Bme18I GGWCC 2 cut(s) 58, 878
BmeRI GACNNNNNGTC 1 cut(s) 835
BmgT120I GGNCC 2 cut(s) 58, 878
BmiI GGNNCC 4 cut(s) 60, 353, 578, 695
BmrFI CCNGG 2 cut(s) 570, 833
BmsI GCATC 3 cut(s) 175, 317, 328
BpmI CTGGAG 1 cut(s) 1141
BpuEI CTTGAG 1 cut(s) 1066
BpuMI CCSGG 1 cut(s) 570
Bsa29I ATCGAT 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 3 cut(s) 167, 605, 749
BsaWI WCCGGW 2 cut(s) 55, 805
BsaXI ACNNNNNCTCC 2 cut(s) 910, 940
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 413, 703, 755
Bse1I ACTGG 3 cut(s) 580, 1057, 1124
Bse3DI GCAATG 1 cut(s) 637
BseAI TCCGGA 1 cut(s) 55
BseBI CCWGG 1 cut(s) 833
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 3 cut(s) 167, 605, 749
BseGI GGATG 3 cut(s) 121, 695, 775
BseLI CCNNNNNNNGG 4 cut(s) 101, 413, 703, 755
BseMI GCAATG 1 cut(s) 637
BseMII CTCAG 3 cut(s) 249, 633, 638
BseNI ACTGG 3 cut(s) 580, 1057, 1124
BseRI GAGGAG 4 cut(s) 81, 84, 260, 945
BseXI GCAGC 1 cut(s) 812
BsgI GTGCAG 3 cut(s) 130, 261, 354
Bsh1236I CGCG 2 cut(s) 685, 719
BshVI ATCGAT 1 cut(s) 363
BsiSI CCGG 3 cut(s) 56, 569, 806
BslFI GGGAC 3 cut(s) 6, 482, 1059
BslI CCNNNNNNNGG 4 cut(s) 101, 413, 703, 755
BsmAI GTCTC 2 cut(s) 299, 638
BsmBI CGTCTC 1 cut(s) 638
BsmFI GGGAC 3 cut(s) 6, 482, 1059
BsmI GAATGC 1 cut(s) 661
Bso31I GGTCTC 1 cut(s) 299
Bsp13I TCCGGA 1 cut(s) 55
Bsp143I GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 3 cut(s) 25, 685, 717
BspCNI CTCAG 3 cut(s) 248, 634, 637
BspDI ATCGAT 1 cut(s) 363
BspEI TCCGGA 1 cut(s) 55
BspFNI CGCG 2 cut(s) 685, 719
BspLI GGNNCC 4 cut(s) 60, 353, 578, 695
BspPI GGATC 4 cut(s) 314, 359, 396, 797
BspTNI GGTCTC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 637
BsrI ACTGG 3 cut(s) 580, 1057, 1124
BssECI CCNNGG 3 cut(s) 167, 605, 749
BssMI GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
BssNAI GTATAC 1 cut(s) 122
BssT1I CCWWGG 3 cut(s) 167, 605, 749
Bst1107I GTATAC 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 3 cut(s) 496, 710, 954
BstDEI CTNAG 3 cut(s) 235, 624, 642
BstDSI CCRYGG 1 cut(s) 167
BstENI CCTNNNNNAGG 1 cut(s) 753
BstF5I GGATG 3 cut(s) 121, 695, 775
BstFNI CGCG 2 cut(s) 685, 719
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 2 cut(s) 273, 721
BstKTI GATC 6 cut(s) 322, 367, 391, 805, 925, 1067
BstMAI GTCTC 2 cut(s) 299, 638
BstMBI GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
BstMWI GCNNNNNNNGC 2 cut(s) 248, 1077
BstNI CCWGG 1 cut(s) 833
BstSCI CCNGG 2 cut(s) 568, 831
BstUI CGCG 2 cut(s) 685, 719
BstV1I GCAGC 1 cut(s) 812
BstXI CCANNNNNNTGG 1 cut(s) 729
BstZ17I GTATAC 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 363
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 167
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 3 cut(s) 121, 695, 775
BtsIMutI CAGTG 3 cut(s) 492, 587, 959
CfoI GCGC 2 cut(s) 273, 721
Cfr13I GGNCC 2 cut(s) 58, 878
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 142, 397
CviAII CATG 2 cut(s) 168, 660
CviQI GTAC 2 cut(s) 142, 397
DdeI CTNAG 3 cut(s) 235, 624, 642
DpnI GATC 6 cut(s) 321, 366, 390, 804, 924, 1066
DpnII GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
DriI GACNNNNNGTC 1 cut(s) 835
Eam1105I GACNNNNNGTC 1 cut(s) 835
Eco130I CCWWGG 3 cut(s) 167, 605, 749
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 58, 878
Eco47III AGCGCT 1 cut(s) 272
EcoNI CCTNNNNNAGG 1 cut(s) 753
EcoRII CCWGG 1 cut(s) 831
EcoT14I CCWWGG 3 cut(s) 167, 605, 749
EcoT22I ATGCAT 1 cut(s) 661
ErhI CCWWGG 3 cut(s) 167, 605, 749
Esp3I CGTCTC 1 cut(s) 638
FaeI CATG 2 cut(s) 171, 663
FaqI GGGAC 3 cut(s) 6, 482, 1059
FatI CATG 2 cut(s) 167, 659
FauI CCCGC 2 cut(s) 32, 678
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 826
FokI GGATG 3 cut(s) 128, 702, 782
Fsp4HI GCNGC 1 cut(s) 826
FspBI CTAG 1 cut(s) 882
GlaI GCGC 2 cut(s) 272, 720
GluI GCNGC 1 cut(s) 826
GsuI CTGGAG 1 cut(s) 1141
HaeII RGCGCY 1 cut(s) 274
HapII CCGG 3 cut(s) 56, 569, 806
HgaI GACGC 1 cut(s) 314
HhaI GCGC 2 cut(s) 273, 721
Hin1II CATG 2 cut(s) 171, 663
Hin6I GCGC 2 cut(s) 271, 719
HinP1I GCGC 2 cut(s) 271, 719
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 5 cut(s) 13, 106, 233, 680, 836
HpaII CCGG 3 cut(s) 56, 569, 806
HphI GGTGA 3 cut(s) 71, 706, 1046
Hpy166II GTNNAC 1 cut(s) 122
Hpy188I TCNGA 3 cut(s) 466, 948, 988
Hpy188III TCNNGA 8 cut(s) 44, 56, 392, 407, 479, 596, 767, 1083
Hpy8I GTNNAC 1 cut(s) 122
HpyAV CCTTC 5 cut(s) 108, 169, 413, 666, 749
HpyCH4III ACNGT 3 cut(s) 496, 710, 954
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 8 cut(s) 147, 188, 242, 263, 341, 371, 659, 1090
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 1077
HpyF3I CTNAG 3 cut(s) 235, 624, 642
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 2 cut(s) 171, 663
HspAI GCGC 2 cut(s) 271, 719
Kpn2I TCCGGA 1 cut(s) 55
Kzo9I GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
LmnI GCTCC 3 cut(s) 409, 571, 1016
Lsp1109I GCAGC 1 cut(s) 812
LweI GCATC 3 cut(s) 175, 317, 328
MaeI CTAG 1 cut(s) 882
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 3 cut(s) 527, 596, 1112
MalI GATC 6 cut(s) 321, 366, 390, 804, 924, 1066
MboI GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
MboII GAAGA 2 cut(s) 664, 677
MfeI CAATTG 1 cut(s) 223
MluCI AATT 7 cut(s) 193, 223, 514, 618, 814, 862, 982
MlyI GAGTC 4 cut(s) 115, 227, 689, 845
Mph1103I ATGCAT 1 cut(s) 661
MroI TCCGGA 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 51
MseI TTAA 1 cut(s) 378
MspI CCGG 3 cut(s) 56, 569, 806
MspR9I CCNGG 2 cut(s) 570, 833
MunI CAATTG 1 cut(s) 223
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 1 cut(s) 833
MvnI CGCG 2 cut(s) 685, 719
MwoI GCNNNNNNNGC 2 cut(s) 248, 1077
NciI CCSGG 1 cut(s) 570
NcoI CCATGG 1 cut(s) 167
NdeII GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
NlaIII CATG 2 cut(s) 171, 663
NlaIV GGNNCC 4 cut(s) 60, 353, 578, 695
NmuCI GTSAC 2 cut(s) 527, 596
NsiI ATGCAT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PctI GAATGC 1 cut(s) 661
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 827
PleI GAGTC 4 cut(s) 114, 227, 688, 844
PpsI GAGTC 4 cut(s) 114, 227, 688, 844
Psp6I CCWGG 1 cut(s) 831
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 4 cut(s) 60, 353, 578, 695
PspPI GGNCC 2 cut(s) 58, 878
RsaI GTAC 2 cut(s) 143, 398
RsaNI GTAC 2 cut(s) 142, 397
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 1 cut(s) 826
Sau3AI GATC 6 cut(s) 319, 364, 388, 802, 922, 1064
Sau96I GGNCC 2 cut(s) 58, 878
ScaI AGTACT 2 cut(s) 143, 398
SchI GAGTC 4 cut(s) 115, 227, 689, 845
ScrFI CCNGG 2 cut(s) 570, 833
SfaNI GCATC 3 cut(s) 175, 317, 328
SinI GGWCC 2 cut(s) 58, 878
SmlI CTYRAG 1 cut(s) 1081
SmoI CTYRAG 1 cut(s) 1081
Sse9I AATT 7 cut(s) 193, 223, 514, 618, 814, 862, 982
SsiI CCGC 3 cut(s) 25, 685, 717
SspI AATATT 2 cut(s) 328, 461
SspMI CTAG 1 cut(s) 882
StyD4I CCNGG 2 cut(s) 568, 831
StyI CCWWGG 3 cut(s) 167, 605, 749
TaaI ACNGT 3 cut(s) 496, 710, 954
TaiI ACGT 1 cut(s) 152
TaqI TCGA 2 cut(s) 363, 391
TasI AATT 7 cut(s) 193, 223, 514, 618, 814, 862, 982
TatI WGTACW 2 cut(s) 141, 396
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TscAI CASTG 3 cut(s) 499, 587, 959
TseFI GTSAC 2 cut(s) 527, 596
TseI GCWGC 1 cut(s) 825
Tsp45I GTSAC 2 cut(s) 527, 596
TspDTI ATGAA 3 cut(s) 5, 498, 951
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 3 cut(s) 499, 587, 959
VpaK11BI GGWCC 2 cut(s) 58, 878
XagI CCTNNNNNAGG 1 cut(s) 753
XapI RAATTY 2 cut(s) 514, 862
XmiI GTMKAC 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 1 cut(s) 882
ZrmI AGTACT 2 cut(s) 143, 398
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.