Rh1DG031500

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014785
Physical Location & Seq
Reverse (-)
4617256 .. 4620387
3132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG031500.1

Sequence Viewer

Length: 1158 bp
ATGGGTAAATGGGGACATCGTCCCAATCGCAGGTTCTTCCGTCAAGAAAAGCTTCCAAGCCCATTCCCTCTTCCTTTTCACCCTCGACCTCCTCCCCCTTCAGAGTTTTGGCGGGATGGTGTACCTCAATGGGAAAAGGAATACTGCACTTTGATCGGGTCCATACCATGGTGGAAGGTGGTAGATGCCAAGAATTATATGTATGGCAGTAGTAATGTACTCAATTGGGACGATTCAGCTGGTGAGGAGGCATTTCAAAATGCGAAAAAGCGCTTTTGGGCAGACATCAACGGCCTCCACTGTGACATCTCTCTACCTGCTTCAGATATTTATATTGATGAAATAGATTGGAACCCTTTCATCGATCCTGATATCGTGAAGGAAGTGGATCGTGAGTACTTTGCTCCTGATGATGGAGAAGAAAATGACAAGTTGGGGCAGAAAAAGAGGAAGACAGAACATTCAGCTATTATTCCTTTAGATGGACATAACTGTCTTCCAGATACTCCTACAAATCCTTGGGAATGTGACAATATGCAAAGTAGTGGGGATTTGAAAAATGAAGCAGAGGGCTGGAACCAGGGGAATAATCATACAGATTTGGTTAGCGGTGAAGTCAACTCCTGGGAACGCCTCATTACTCAGAGCAATGAAAGAATGGAAAATACTGCATGGGAAGATAGGGATAAATCATGGAGATGGAACCAGATGGCAAAAAACAGTCTCTCAAAGAATTGGGATGATGGTGGCAATCCTTGGGCACGGGACTGTCAGGGTTTTACCCATGTGAAAGTTAATAATAGATGGGGTGATCCCTTAAATAAGTCTTGGGGCTCCAACCAGCCGGAGACAAAGAATTTGGATCATGGTGAAAATCCTTGGGAGTGTGGTCCTAGTCAATATAATGAAGCTTTAACAAAAGATCGAGGATGGAAAGATTGTGGAGGTTGGAAACAGTGTGACAGCCGTATTAACCAGAGAAATAATTTGGATTTTAGGATAAAAGGTAGCAGATGGGGAACTACAAATCACAGTGGCCAGAAGAGGGAACGGTCTCACCAATATATAGCAGGATCCCAAAGCTCAAGATTTCAAGGGAGTGATAGTCAAACAGGTGGATACTGGAGGAGGGCAAACAATAGAAAGAGGCTCAGCTGA

Protein Analysis

385

Amino Acids

44.77

Weight (kDa)

6.1

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014785)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02130 FvH4_7g02130
malus_domestica MD02G1297200.v1.1
prunus_persica Prupe.2G026900_v2.0.a1
pyrus_communis pycom02g25040 pycom07g02040
rosa_chinensis RchiOBHm_Chr1g0319371
rosa_laevigata RLG00000030492
rosa_multiflora Rmu_sc0005870.1_g000004 Rmu_sc0014506.1_g000002
rosa_roxburghii Rroxscaffold_4G00328530
rosa_rugosa Rorug01G0027200
rosa_samantha Rh1AG039400 Rh1BG036200 Rh1CG038300 Rh1DG031500
rosa_wichuraiana Rw1G003150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 492
Acc36I ACCTGC 2 cut(s) 21, 325
AccB7I CCANNNNNTGG 1 cut(s) 168
AciI CCGC 2 cut(s) 112, 609
AclWI GGATC 6 cut(s) 359, 396, 806, 870, 1068, 1081
AcoI YGGCCR 1 cut(s) 1036
AcsI RAATTY 1 cut(s) 856
AcuI CTGAAG 2 cut(s) 84, 306
AfaI GTAC 3 cut(s) 123, 219, 398
AfeI AGCGCT 1 cut(s) 272
AfiI CCNNNNNNNGG 5 cut(s) 30, 168, 413, 482, 1045
AgsI TTSAA 3 cut(s) 257, 556, 1094
AjnI CCWGG 2 cut(s) 579, 623
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 6 cut(s) 52, 239, 467, 911, 1083, 1155
AluI AGCT 6 cut(s) 52, 239, 467, 911, 1083, 1155
Alw26I GTCTC 3 cut(s) 728, 842, 1059
AlwI GGATC 6 cut(s) 359, 396, 806, 870, 1068, 1081
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 2 cut(s) 292, 1036
ApoI RAATTY 1 cut(s) 856
ArsI GACNNNNNNTTYG 2 cut(s) 841, 873
Asp700I GAANNNNTTC 2 cut(s) 51, 356
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 2 cut(s) 159, 890
AsuHPI GGTGA 6 cut(s) 71, 254, 623, 821, 881, 1049
AvaII GGWCC 2 cut(s) 159, 890
BaeGI GKGCMC 1 cut(s) 763
BalI TGGCCA 1 cut(s) 1038
BamHI GGATCC 1 cut(s) 1073
BanII GRGCYC 1 cut(s) 836
BbsI GAAGAC 2 cut(s) 458, 488
BccI CCATC 9 cut(s) 110, 407, 476, 693, 703, 737, 798, 924, 1008
BceAI ACGGC 2 cut(s) 307, 951
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 2 cut(s) 581, 625
BciVI GTATCC 1 cut(s) 1112
BcoDI GTCTC 3 cut(s) 728, 842, 1059
BfaI CTAG 1 cut(s) 894
BfoI RGCGCY 1 cut(s) 274
BfuAI ACCTGC 2 cut(s) 21, 325
BfuI GTATCC 1 cut(s) 1112
BlpI GCTNAGC 1 cut(s) 1151
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 581, 625
Bme18I GGWCC 2 cut(s) 159, 890
BmgT120I GGNCC 2 cut(s) 159, 890
BmiI GGNNCC 6 cut(s) 160, 353, 578, 704, 835, 1075
BmrFI CCNGG 2 cut(s) 581, 625
BmsI GCATC 1 cut(s) 175
BpiI GAAGAC 2 cut(s) 458, 488
BpmI CTGGAG 1 cut(s) 1144
Bpu1102I GCTNAGC 1 cut(s) 1151
BpuEI CTTGAG 1 cut(s) 1069
Bsa29I ATCGAT 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 1059
BsaJI CCNNGG 6 cut(s) 167, 518, 580, 624, 755, 878
BsaXI ACNNNNNCTCC 2 cut(s) 1090, 1120
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 168, 413, 482, 1045
Bse1I ACTGG 1 cut(s) 1127
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 2 cut(s) 581, 625
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 6 cut(s) 167, 518, 580, 624, 755, 878
BseGI GGATG 3 cut(s) 121, 745, 935
BseLI CCNNNNNNNGG 5 cut(s) 30, 168, 413, 482, 1045
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 1 cut(s) 1127
BseRI GAGGAG 3 cut(s) 81, 260, 1141
BseSI GKGCMC 1 cut(s) 763
BsgI GTGCAG 1 cut(s) 130
BshFI GGCC 2 cut(s) 294, 1038
BshVI ATCGAT 1 cut(s) 363
BsiSI CCGG 1 cut(s) 845
BslFI GGGAC 4 cut(s) 6, 27, 242, 779
BslI CCNNNNNNNGG 5 cut(s) 30, 168, 413, 482, 1045
BsmAI GTCTC 3 cut(s) 728, 842, 1059
BsmFI GGGAC 4 cut(s) 6, 27, 242, 779
BsnI GGCC 2 cut(s) 294, 1038
Bso31I GGTCTC 1 cut(s) 1059
Bsp1286I GDGCHC 2 cut(s) 763, 836
Bsp143I GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
Bsp1720I GCTNAGC 1 cut(s) 1151
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 2 cut(s) 112, 609
BspANI GGCC 2 cut(s) 294, 1038
BspCNI CTCAG 1 cut(s) 655
BspDI ATCGAT 1 cut(s) 363
BspLI GGNNCC 6 cut(s) 160, 353, 578, 704, 835, 1075
BspMI ACCTGC 2 cut(s) 21, 325
BspPI GGATC 6 cut(s) 359, 396, 806, 870, 1068, 1081
BspTNI GGTCTC 1 cut(s) 1059
BsrDI GCAATG 1 cut(s) 655
BsrI ACTGG 1 cut(s) 1127
BssECI CCNNGG 6 cut(s) 167, 518, 580, 624, 755, 878
BssMI GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
BssT1I CCWWGG 4 cut(s) 167, 518, 755, 878
Bst2UI CCWGG 2 cut(s) 581, 625
Bst4CI ACNGT 7 cut(s) 302, 494, 722, 770, 957, 1034, 1053
Bst6I CTCTTC 2 cut(s) 75, 1037
BstDEI CTNAG 2 cut(s) 642, 1151
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 3 cut(s) 121, 745, 935
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 7 cut(s) 156, 367, 391, 814, 865, 925, 1076
BstMAI GTCTC 3 cut(s) 728, 842, 1059
BstMBI GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
BstNI CCWGG 2 cut(s) 581, 625
BstSCI CCNGG 2 cut(s) 579, 623
BstSLI GKGCMC 1 cut(s) 763
BstV2I GAAGAC 2 cut(s) 458, 488
BstX2I RGATCY 1 cut(s) 1073
BstYI RGATCY 1 cut(s) 1073
Bsu15I ATCGAT 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1112
BsuRI GGCC 2 cut(s) 294, 1038
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 3 cut(s) 121, 745, 935
BtsIMutI CAGTG 3 cut(s) 298, 962, 1039
BveI ACCTGC 2 cut(s) 21, 325
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 159, 890
ClaI ATCGAT 1 cut(s) 363
Csp6I GTAC 3 cut(s) 122, 218, 397
CviAII CATG 5 cut(s) 168, 672, 693, 785, 866
CviQI GTAC 3 cut(s) 122, 218, 397
DdeI CTNAG 2 cut(s) 642, 1151
DpnI GATC 7 cut(s) 155, 366, 390, 813, 864, 924, 1075
DpnII GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
DrdI GACNNNNNNGTC 1 cut(s) 492
DseDI GACNNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 1036
Eam1104I CTCTTC 2 cut(s) 75, 1037
EarI CTCTTC 2 cut(s) 75, 1037
Eco130I CCWWGG 4 cut(s) 167, 518, 755, 878
Eco24I GRGCYC 1 cut(s) 836
Eco31I GGTCTC 1 cut(s) 1059
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 159, 890
Eco47III AGCGCT 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 84, 306
EcoRII CCWGG 2 cut(s) 579, 623
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 4 cut(s) 167, 518, 755, 878
EcoT38I GRGCYC 1 cut(s) 836
ErhI CCWWGG 4 cut(s) 167, 518, 755, 878
FaeI CATG 5 cut(s) 171, 675, 696, 788, 869
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 4 cut(s) 6, 27, 242, 779
FatI CATG 5 cut(s) 167, 671, 692, 784, 865
FauI CCCGC 1 cut(s) 105
FokI GGATG 3 cut(s) 128, 752, 942
FriOI GRGCYC 1 cut(s) 836
FspBI CTAG 1 cut(s) 894
GlaI GCGC 1 cut(s) 272
GsuI CTGGAG 1 cut(s) 1144
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 2 cut(s) 294, 1038
HapII CCGG 1 cut(s) 845
HhaI GCGC 1 cut(s) 273
Hin1II CATG 5 cut(s) 171, 675, 696, 788, 869
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 2 cut(s) 50, 909
HinfI GANTC 1 cut(s) 233
HpaII CCGG 1 cut(s) 845
HphI GGTGA 6 cut(s) 71, 254, 623, 821, 881, 1049
Hpy166II GTNNAC 2 cut(s) 122, 619
Hpy188I TCNGA 3 cut(s) 103, 325, 645
Hpy188III TCNNGA 7 cut(s) 44, 368, 376, 392, 407, 500, 1086
Hpy8I GTNNAC 2 cut(s) 122, 619
HpyAV CCTTC 3 cut(s) 108, 169, 373
HpyCH4III ACNGT 7 cut(s) 302, 494, 722, 770, 957, 1034, 1053
HpyCH4V TGCA 3 cut(s) 147, 538, 671
HpyF3I CTNAG 2 cut(s) 642, 1151
Hsp92II CATG 5 cut(s) 171, 675, 696, 788, 869
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
LmnI GCTCC 2 cut(s) 409, 839
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 894
MaeIII GTNAC 3 cut(s) 302, 527, 959
MalI GATC 7 cut(s) 155, 366, 390, 813, 864, 924, 1075
MboI GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
MboII GAAGA 7 cut(s) 28, 62, 431, 463, 488, 689, 1054
MfeI CAATTG 1 cut(s) 223
MflI RGATCY 1 cut(s) 1073
MhlI GDGCHC 2 cut(s) 763, 836
MlsI TGGCCA 1 cut(s) 1038
MluCI AATT 5 cut(s) 193, 223, 733, 856, 985
MluNI TGGCCA 1 cut(s) 1038
MmeI TCCRAC 2 cut(s) 861, 929
Mox20I TGGCCA 1 cut(s) 1038
MroXI GAANNNNTTC 2 cut(s) 51, 356
MscI TGGCCA 1 cut(s) 1038
MseI TTAA 4 cut(s) 795, 818, 914, 972
MslI CAYNNNNRTG 1 cut(s) 697
Msp20I TGGCCA 1 cut(s) 1038
MspA1I CMGCKG 2 cut(s) 239, 1155
MspI CCGG 1 cut(s) 845
MspR9I CCNGG 2 cut(s) 581, 625
MunI CAATTG 1 cut(s) 223
MvaI CCWGG 2 cut(s) 581, 625
NcoI CCATGG 1 cut(s) 167
NdeII GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
NlaIII CATG 5 cut(s) 171, 675, 696, 788, 869
NlaIV GGNNCC 6 cut(s) 160, 353, 578, 704, 835, 1075
NmuCI GTSAC 3 cut(s) 302, 527, 959
PdmI GAANNNNTTC 2 cut(s) 51, 356
PfeI GAWTC 1 cut(s) 233
PflFI GACNNNGTC 1 cut(s) 18
PflMI CCANNNNNTGG 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 579, 623
PspGI CCWGG 2 cut(s) 579, 623
PspN4I GGNNCC 6 cut(s) 160, 353, 578, 704, 835, 1075
PspPI GGNCC 2 cut(s) 159, 890
PsuI RGATCY 1 cut(s) 1073
PsyI GACNNNGTC 1 cut(s) 18
PvuII CAGCTG 2 cut(s) 239, 1155
RsaI GTAC 3 cut(s) 123, 219, 398
RsaNI GTAC 3 cut(s) 122, 218, 397
RseI CAYNNNNRTG 1 cut(s) 697
SaqAI TTAA 4 cut(s) 795, 818, 914, 972
Sau3AI GATC 7 cut(s) 153, 364, 388, 811, 862, 922, 1073
Sau96I GGNCC 2 cut(s) 159, 890
ScaI AGTACT 1 cut(s) 398
ScrFI CCNGG 2 cut(s) 581, 625
SduI GDGCHC 2 cut(s) 763, 836
SfaNI GCATC 1 cut(s) 175
SinI GGWCC 2 cut(s) 159, 890
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 1084
SmoI CTYRAG 1 cut(s) 1084
Sse9I AATT 5 cut(s) 193, 223, 733, 856, 985
SsiI CCGC 2 cut(s) 112, 609
SspMI CTAG 1 cut(s) 894
StyD4I CCNGG 2 cut(s) 579, 623
StyI CCWWGG 4 cut(s) 167, 518, 755, 878
TaaI ACNGT 7 cut(s) 302, 494, 722, 770, 957, 1034, 1053
TaqI TCGA 3 cut(s) 85, 363, 925
TasI AATT 5 cut(s) 193, 223, 733, 856, 985
TatI WGTACW 2 cut(s) 217, 396
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 4 cut(s) 795, 818, 914, 972
Tru9I TTAA 4 cut(s) 795, 818, 914, 972
TscAI CASTG 3 cut(s) 305, 962, 1039
TseFI GTSAC 3 cut(s) 302, 527, 959
Tsp45I GTSAC 3 cut(s) 302, 527, 959
TspDTI ATGAA 5 cut(s) 349, 354, 576, 666, 921
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 3 cut(s) 305, 962, 1039
Tth111I GACNNNGTC 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 159, 890
XapI RAATTY 1 cut(s) 856
XmnI GAANNNNTTC 2 cut(s) 51, 356
XspI CTAG 1 cut(s) 894
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.