Regulator of Vps4 activity in the MVB pathway
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 1466, 1791 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 997, 1695 |
| AciI | CCGC | 4 cut(s) | 1013, 1186, 1470, 1798 |
| AclWI | GGATC | 3 cut(s) | 173, 243, 550 |
| AcoI | YGGCCR | 1 cut(s) | 68 |
| AcsI | RAATTY | 2 cut(s) | 355, 555 |
| AcuI | CTGAAG | 4 cut(s) | 575, 863, 1202, 1343 |
| AcyI | GRCGYC | 1 cut(s) | 1792 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 997, 1695 |
| AflII | CTTAAG | 2 cut(s) | 265, 548 |
| AflIII | ACRYGT | 2 cut(s) | 1335, 1759 |
| AgeI | ACCGGT | 1 cut(s) | 983 |
| AgsI | TTSAA | 7 cut(s) | 48, 512, 980, 1229, 1424, 1646, 1675 |
| AhlI | ACTAGT | 1 cut(s) | 1487 |
| AluBI | AGCT | 3 cut(s) | 1099, 1720, 1769 |
| AluI | AGCT | 3 cut(s) | 1099, 1720, 1769 |
| Alw21I | GWGCWC | 1 cut(s) | 1741 |
| Alw26I | GTCTC | 1 cut(s) | 1690 |
| AlwI | GGATC | 3 cut(s) | 173, 243, 550 |
| AoxI | GGCC | 2 cut(s) | 68, 1629 |
| ApeKI | GCWGC | 5 cut(s) | 344, 626, 1410, 1589, 1766 |
| ApoI | RAATTY | 2 cut(s) | 355, 555 |
| AsiGI | ACCGGT | 1 cut(s) | 983 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 387 |
| AspLEI | GCGC | 3 cut(s) | 1505, 1666, 1794 |
| AspS9I | GGNCC | 2 cut(s) | 986, 1630 |
| AsuC2I | CCSGG | 1 cut(s) | 11 |
| AsuHPI | GGTGA | 3 cut(s) | 46, 448, 1556 |
| AsuII | TTCGAA | 1 cut(s) | 359 |
| AvaII | GGWCC | 1 cut(s) | 986 |
| BalI | TGGCCA | 1 cut(s) | 70 |
| BanI | GGYRCC | 2 cut(s) | 1466, 1791 |
| BbsI | GAAGAC | 1 cut(s) | 163 |
| Bbv12I | GWGCWC | 1 cut(s) | 1741 |
| BbvCI | CCTCAGC | 1 cut(s) | 1262 |
| BbvI | GCAGC | 5 cut(s) | 331, 613, 1397, 1576, 1778 |
| BccI | CCATC | 3 cut(s) | 1616, 1727, 1809 |
| BclI | TGATCA | 1 cut(s) | 1200 |
| BcnI | CCSGG | 1 cut(s) | 11 |
| BcoDI | GTCTC | 1 cut(s) | 1690 |
| BcuI | ACTAGT | 1 cut(s) | 1487 |
| BfaI | CTAG | 2 cut(s) | 1133, 1488 |
| BfmI | CTRYAG | 3 cut(s) | 276, 440, 763 |
| BfoI | RGCGCY | 1 cut(s) | 1795 |
| BfrI | CTTAAG | 2 cut(s) | 265, 548 |
| BglII | AGATCT | 3 cut(s) | 269, 280, 1177 |
| BisI | GCNGC | 5 cut(s) | 345, 627, 1411, 1590, 1767 |
| BlsI | GCNGC | 5 cut(s) | 346, 628, 1412, 1591, 1768 |
| BmcAI | AGTACT | 2 cut(s) | 769, 1362 |
| Bme1390I | CCNGG | 1 cut(s) | 11 |
| Bme18I | GGWCC | 1 cut(s) | 986 |
| BmgT120I | GGNCC | 2 cut(s) | 986, 1630 |
| BmiI | GGNNCC | 4 cut(s) | 970, 988, 1468, 1793 |
| BmrFI | CCNGG | 1 cut(s) | 11 |
| BmsI | GCATC | 4 cut(s) | 322, 823, 1390, 1437 |
| BpiI | GAAGAC | 1 cut(s) | 163 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1548, 1580 |
| BpmI | CTGGAG | 1 cut(s) | 594 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1262 |
| Bpu14I | TTCGAA | 1 cut(s) | 359 |
| BpuEI | CTTGAG | 3 cut(s) | 1370, 1460, 1520 |
| BpuMI | CCSGG | 1 cut(s) | 11 |
| Bsa29I | ATCGAT | 1 cut(s) | 1245 |
| BsaHI | GRCGYC | 1 cut(s) | 1792 |
| BsaJI | CCNNGG | 2 cut(s) | 777, 1404 |
| BsaWI | WCCGGW | 4 cut(s) | 199, 458, 505, 983 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 997, 1695 |
| Bse118I | RCCGGY | 1 cut(s) | 983 |
| Bse1I | ACTGG | 4 cut(s) | 577, 1036, 1368, 1391 |
| Bse3DI | GCAATG | 1 cut(s) | 935 |
| BseCI | ATCGAT | 1 cut(s) | 1245 |
| BseDI | CCNNGG | 2 cut(s) | 777, 1404 |
| BseGI | GGATG | 1 cut(s) | 142 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 997, 1695 |
| BseMI | GCAATG | 1 cut(s) | 935 |
| BseMII | CTCAG | 1 cut(s) | 1276 |
| BseNI | ACTGG | 4 cut(s) | 577, 1036, 1368, 1391 |
| BseRI | GAGGAG | 1 cut(s) | 1730 |
| BseXI | GCAGC | 5 cut(s) | 331, 613, 1397, 1576, 1778 |
| Bsh1285I | CGRYCG | 1 cut(s) | 204 |
| BshFI | GGCC | 2 cut(s) | 70, 1631 |
| BshNI | GGYRCC | 2 cut(s) | 1466, 1791 |
| BshTI | ACCGGT | 1 cut(s) | 983 |
| BshVI | ATCGAT | 1 cut(s) | 1245 |
| BsiEI | CGRYCG | 1 cut(s) | 204 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1741 |
| BsiSI | CCGG | 6 cut(s) | 11, 200, 459, 506, 984, 1748 |
| BslFI | GGGAC | 2 cut(s) | 972, 1443 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 997, 1695 |
| BsmAI | GTCTC | 1 cut(s) | 1690 |
| BsmFI | GGGAC | 2 cut(s) | 972, 1443 |
| BsmI | GAATGC | 1 cut(s) | 1266 |
| BsnI | GGCC | 2 cut(s) | 70, 1631 |
| Bsp119I | TTCGAA | 1 cut(s) | 359 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1741 |
| Bsp1407I | TGTACA | 2 cut(s) | 186, 1299 |
| BspACI | CCGC | 4 cut(s) | 1013, 1186, 1470, 1798 |
| BspANI | GGCC | 2 cut(s) | 70, 1631 |
| BspCNI | CTCAG | 1 cut(s) | 1275 |
| BspDI | ATCGAT | 1 cut(s) | 1245 |
| BspHI | TCATGA | 1 cut(s) | 1203 |
| BspLI | GGNNCC | 4 cut(s) | 970, 988, 1468, 1793 |
| BspPI | GGATC | 3 cut(s) | 173, 243, 550 |
| BspQI | GCTCTTC | 2 cut(s) | 435, 882 |
| BspT104I | TTCGAA | 1 cut(s) | 359 |
| BspT107I | GGYRCC | 2 cut(s) | 1466, 1791 |
| BspTI | CTTAAG | 2 cut(s) | 265, 548 |
| BsrDI | GCAATG | 1 cut(s) | 935 |
| BsrFI | RCCGGY | 1 cut(s) | 983 |
| BsrGI | TGTACA | 2 cut(s) | 186, 1299 |
| BsrI | ACTGG | 4 cut(s) | 577, 1036, 1368, 1391 |
| BssAI | RCCGGY | 1 cut(s) | 983 |
| BssECI | CCNNGG | 2 cut(s) | 777, 1404 |
| BssNI | GRCGYC | 1 cut(s) | 1792 |
| Bst4CI | ACNGT | 3 cut(s) | 191, 656, 764 |
| Bst6I | CTCTTC | 3 cut(s) | 435, 882, 1670 |
| BstACI | GRCGYC | 1 cut(s) | 1792 |
| BstAFI | CTTAAG | 2 cut(s) | 265, 548 |
| BstAUI | TGTACA | 2 cut(s) | 186, 1299 |
| BstBI | TTCGAA | 1 cut(s) | 359 |
| BstC8I | GCNNGC | 3 cut(s) | 395, 823, 1379 |
| BstDEI | CTNAG | 2 cut(s) | 336, 1262 |
| BstF5I | GGATG | 1 cut(s) | 142 |
| BstH2I | RGCGCY | 1 cut(s) | 1795 |
| BstHHI | GCGC | 3 cut(s) | 1505, 1666, 1794 |
| BstMAI | GTCTC | 1 cut(s) | 1690 |
| BstMCI | CGRYCG | 1 cut(s) | 204 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 341, 1387, 1661 |
| BstNSI | RCATGY | 4 cut(s) | 225, 825, 1339, 1763 |
| BstSCI | CCNGG | 1 cut(s) | 9 |
| BstSFI | CTRYAG | 3 cut(s) | 276, 440, 763 |
| BstV1I | GCAGC | 5 cut(s) | 331, 613, 1397, 1576, 1778 |
| BstV2I | GAAGAC | 1 cut(s) | 163 |
| BstX2I | RGATCY | 3 cut(s) | 269, 280, 1177 |
| BstYI | RGATCY | 3 cut(s) | 269, 280, 1177 |
| Bsu15I | ATCGAT | 1 cut(s) | 1245 |
| BsuRI | GGCC | 2 cut(s) | 70, 1631 |
| BsuTUI | ATCGAT | 1 cut(s) | 1245 |
| BtsCI | GGATG | 1 cut(s) | 142 |
| BtsI | GCAGTG | 1 cut(s) | 453 |
| BtsIMutI | CAGTG | 2 cut(s) | 453, 1398 |
| Cac8I | GCNNGC | 3 cut(s) | 395, 823, 1379 |
| CciI | TCATGA | 1 cut(s) | 1203 |
| CfoI | GCGC | 3 cut(s) | 1505, 1666, 1794 |
| Cfr10I | RCCGGY | 1 cut(s) | 983 |
| Cfr13I | GGNCC | 2 cut(s) | 986, 1630 |
| ClaI | ATCGAT | 1 cut(s) | 1245 |
| CspAI | ACCGGT | 1 cut(s) | 983 |
| DdeI | CTNAG | 2 cut(s) | 336, 1262 |
| DinI | GGCGCC | 1 cut(s) | 1793 |
| DraI | TTTAAA | 1 cut(s) | 1080 |
| EaeI | YGGCCR | 1 cut(s) | 68 |
| Eam1104I | CTCTTC | 3 cut(s) | 435, 882, 1670 |
| EarI | CTCTTC | 3 cut(s) | 435, 882, 1670 |
| EciI | GGCGGA | 1 cut(s) | 1787 |
| Eco32I | GATATC | 2 cut(s) | 153, 1243 |
| Eco47I | GGWCC | 1 cut(s) | 986 |
| Eco57I | CTGAAG | 4 cut(s) | 575, 863, 1202, 1343 |
| EcoRI | GAATTC | 1 cut(s) | 555 |
| EcoRV | GATATC | 2 cut(s) | 153, 1243 |
| EcoT22I | ATGCAT | 1 cut(s) | 1810 |
| EgeI | GGCGCC | 1 cut(s) | 1793 |
| EheI | GGCGCC | 1 cut(s) | 1793 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 107, 139, 473, 505, 1209, 1241 |
| FaqI | GGGAC | 2 cut(s) | 972, 1443 |
| FbaI | TGATCA | 1 cut(s) | 1200 |
| Fnu4HI | GCNGC | 5 cut(s) | 345, 627, 1411, 1590, 1767 |
| FokI | GGATG | 1 cut(s) | 149 |
| Fsp4HI | GCNGC | 5 cut(s) | 345, 627, 1411, 1590, 1767 |
| FspBI | CTAG | 2 cut(s) | 1133, 1488 |
| GlaI | GCGC | 3 cut(s) | 1504, 1665, 1793 |
| GluI | GCNGC | 5 cut(s) | 345, 627, 1411, 1590, 1767 |
| GsuI | CTGGAG | 1 cut(s) | 594 |
| HaeII | RGCGCY | 1 cut(s) | 1795 |
| HaeIII | GGCC | 2 cut(s) | 70, 1631 |
| HapII | CCGG | 6 cut(s) | 11, 200, 459, 506, 984, 1748 |
| HhaI | GCGC | 3 cut(s) | 1505, 1666, 1794 |
| Hin1I | GRCGYC | 1 cut(s) | 1792 |
| Hin6I | GCGC | 3 cut(s) | 1503, 1664, 1792 |
| HinP1I | GCGC | 3 cut(s) | 1503, 1664, 1792 |
| HincII | GTYRAC | 2 cut(s) | 208, 1333 |
| HindII | GTYRAC | 2 cut(s) | 208, 1333 |
| HinfI | GANTC | 3 cut(s) | 41, 91, 1129 |
| HpaII | CCGG | 6 cut(s) | 11, 200, 459, 506, 984, 1748 |
| HphI | GGTGA | 3 cut(s) | 46, 448, 1556 |
| Hpy166II | GTNNAC | 5 cut(s) | 187, 208, 252, 842, 1333 |
| Hpy188I | TCNGA | 6 cut(s) | 90, 129, 561, 1093, 1182, 1600 |
| Hpy188III | TCNNGA | 4 cut(s) | 1133, 1204, 1424, 1537 |
| Hpy8I | GTNNAC | 5 cut(s) | 187, 208, 252, 842, 1333 |
| Hpy99I | CGWCG | 3 cut(s) | 110, 321, 698 |
| HpyAV | CCTTC | 6 cut(s) | 625, 1108, 1318, 1621, 1640, 1704 |
| HpyCH4III | ACNGT | 3 cut(s) | 191, 656, 764 |
| HpyCH4IV | ACGT | 2 cut(s) | 316, 696 |
| HpyCH4V | TGCA | 9 cut(s) | 470, 825, 836, 928, 1064, 1252, 1413, 1575, 1808 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 341, 1387, 1661 |
| HpyF3I | CTNAG | 2 cut(s) | 336, 1262 |
| HpySE526I | ACGT | 2 cut(s) | 316, 696 |
| Hsp92I | GRCGYC | 1 cut(s) | 1792 |
| HspAI | GCGC | 3 cut(s) | 1503, 1664, 1792 |
| KasI | GGCGCC | 1 cut(s) | 1791 |
| Ksp22I | TGATCA | 1 cut(s) | 1200 |
| LguI | GCTCTTC | 2 cut(s) | 435, 882 |
| LmnI | GCTCC | 2 cut(s) | 641, 1717 |
| Lsp1109I | GCAGC | 5 cut(s) | 331, 613, 1397, 1576, 1778 |
| LweI | GCATC | 4 cut(s) | 322, 823, 1390, 1437 |
| MaeI | CTAG | 2 cut(s) | 1133, 1488 |
| MaeII | ACGT | 2 cut(s) | 316, 696 |
| MaeIII | GTNAC | 5 cut(s) | 34, 191, 712, 740, 1393 |
| MfeI | CAATTG | 1 cut(s) | 1742 |
| MflI | RGATCY | 3 cut(s) | 269, 280, 1177 |
| MhlI | GDGCHC | 1 cut(s) | 1741 |
| MlsI | TGGCCA | 1 cut(s) | 70 |
| MluCI | AATT | 9 cut(s) | 162, 324, 355, 512, 555, 1371, 1570, 1641, 1742 |
| MluNI | TGGCCA | 1 cut(s) | 70 |
| Mly113I | GGCGCC | 1 cut(s) | 1792 |
| MlyI | GAGTC | 1 cut(s) | 1138 |
| MmeI | TCCRAC | 2 cut(s) | 665, 1713 |
| Mox20I | TGGCCA | 1 cut(s) | 70 |
| Mph1103I | ATGCAT | 1 cut(s) | 1810 |
| MroXI | GAANNNNTTC | 1 cut(s) | 387 |
| MscI | TGGCCA | 1 cut(s) | 70 |
| MseI | TTAA | 9 cut(s) | 266, 353, 486, 549, 947, 1079, 1101, 1190, 1568 |
| Msp20I | TGGCCA | 1 cut(s) | 70 |
| MspCI | CTTAAG | 2 cut(s) | 265, 548 |
| MspI | CCGG | 6 cut(s) | 11, 200, 459, 506, 984, 1748 |
| MspR9I | CCNGG | 1 cut(s) | 11 |
| MunI | CAATTG | 1 cut(s) | 1742 |
| Mva1269I | GAATGC | 1 cut(s) | 1266 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 341, 1387, 1661 |
| NarI | GGCGCC | 1 cut(s) | 1792 |
| NciI | CCSGG | 1 cut(s) | 11 |
| NlaIV | GGNNCC | 4 cut(s) | 970, 988, 1468, 1793 |
| NmuCI | GTSAC | 2 cut(s) | 34, 1393 |
| NsiI | ATGCAT | 1 cut(s) | 1810 |
| NspI | RCATGY | 4 cut(s) | 225, 825, 1339, 1763 |
| NspV | TTCGAA | 1 cut(s) | 359 |
| PaeI | GCATGC | 1 cut(s) | 825 |
| PagI | TCATGA | 1 cut(s) | 1203 |
| PciI | ACATGT | 2 cut(s) | 1335, 1759 |
| PciSI | GCTCTTC | 2 cut(s) | 435, 882 |
| PctI | GAATGC | 1 cut(s) | 1266 |
| PdmI | GAANNNNTTC | 1 cut(s) | 387 |
| PfeI | GAWTC | 2 cut(s) | 41, 91 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 997, 1695 |
| PinAI | ACCGGT | 1 cut(s) | 983 |
| PkrI | GCNGC | 5 cut(s) | 346, 628, 1412, 1591, 1768 |
| PleI | GAGTC | 1 cut(s) | 1137 |
| PluTI | GGCGCC | 1 cut(s) | 1795 |
| PpsI | GAGTC | 1 cut(s) | 1137 |
| PscI | ACATGT | 2 cut(s) | 1335, 1759 |
| PspN4I | GGNNCC | 4 cut(s) | 970, 988, 1468, 1793 |
| PspPI | GGNCC | 2 cut(s) | 986, 1630 |
| PsuI | RGATCY | 3 cut(s) | 269, 280, 1177 |
| SapI | GCTCTTC | 2 cut(s) | 435, 882 |
| SaqAI | TTAA | 9 cut(s) | 266, 353, 486, 549, 947, 1079, 1101, 1190, 1568 |
| SatI | GCNGC | 5 cut(s) | 345, 627, 1411, 1590, 1767 |
| Sau96I | GGNCC | 2 cut(s) | 986, 1630 |
| ScaI | AGTACT | 2 cut(s) | 769, 1362 |
| SchI | GAGTC | 1 cut(s) | 1138 |
| ScrFI | CCNGG | 1 cut(s) | 11 |
| SduI | GDGCHC | 1 cut(s) | 1741 |
| SfaNI | GCATC | 4 cut(s) | 322, 823, 1390, 1437 |
| SfcI | CTRYAG | 3 cut(s) | 276, 440, 763 |
| SfoI | GGCGCC | 1 cut(s) | 1793 |
| SfuI | TTCGAA | 1 cut(s) | 359 |
| SinI | GGWCC | 1 cut(s) | 986 |
| SmlI | CTYRAG | 5 cut(s) | 265, 548, 1385, 1439, 1535 |
| SmoI | CTYRAG | 5 cut(s) | 265, 548, 1385, 1439, 1535 |
| SpeI | ACTAGT | 1 cut(s) | 1487 |
| SphI | GCATGC | 1 cut(s) | 825 |
| Sse9I | AATT | 9 cut(s) | 162, 324, 355, 512, 555, 1371, 1570, 1641, 1742 |
| SsiI | CCGC | 4 cut(s) | 1013, 1186, 1470, 1798 |
| SspDI | GGCGCC | 1 cut(s) | 1791 |
| SspI | AATATT | 1 cut(s) | 955 |
| SspMI | CTAG | 2 cut(s) | 1133, 1488 |
| StyD4I | CCNGG | 1 cut(s) | 9 |
| TaaI | ACNGT | 3 cut(s) | 191, 656, 764 |
| TaiI | ACGT | 2 cut(s) | 319, 699 |
| TaqI | TCGA | 6 cut(s) | 319, 359, 852, 1171, 1245, 1726 |
| TasI | AATT | 9 cut(s) | 162, 324, 355, 512, 555, 1371, 1570, 1641, 1742 |
| TatI | WGTACW | 6 cut(s) | 50, 186, 767, 1279, 1299, 1360 |
| TfiI | GAWTC | 2 cut(s) | 41, 91 |
| Tru1I | TTAA | 9 cut(s) | 266, 353, 486, 549, 947, 1079, 1101, 1190, 1568 |
| Tru9I | TTAA | 9 cut(s) | 266, 353, 486, 549, 947, 1079, 1101, 1190, 1568 |
| TscAI | CASTG | 2 cut(s) | 453, 1398 |
| TseFI | GTSAC | 2 cut(s) | 34, 1393 |
| TseI | GCWGC | 5 cut(s) | 344, 626, 1410, 1589, 1766 |
| Tsp45I | GTSAC | 2 cut(s) | 34, 1393 |
| TspDTI | ATGAA | 8 cut(s) | 17, 722, 888, 1220, 1364, 1368, 1444, 1765 |
| TspGWI | ACGGA | 1 cut(s) | 1510 |
| TspRI | CASTG | 2 cut(s) | 453, 1398 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 997, 1695 |
| Vha464I | CTTAAG | 2 cut(s) | 265, 548 |
| VpaK11BI | GGWCC | 1 cut(s) | 986 |
| XapI | RAATTY | 2 cut(s) | 355, 555 |
| XbaI | TCTAGA | 1 cut(s) | 1132 |
| XceI | RCATGY | 4 cut(s) | 225, 825, 1339, 1763 |
| XmnI | GAANNNNTTC | 1 cut(s) | 387 |
| XspI | CTAG | 2 cut(s) | 1133, 1488 |
| ZrmI | AGTACT | 2 cut(s) | 769, 1362 |
| Zsp2I | ATGCAT | 1 cut(s) | 1810 |