RLG00000030656

Seed biotin-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000328
Physical Location & Seq
Reverse (-)
65156397 .. 65161753
5357 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030656

Sequence Viewer

Length: 975 bp
ATGGAGGAAAGCTACTTCGTCTATGTAATCTTGCTGATGGAGATCTTCAACATCAGTGCATGTAAGGTTGTACTGCATGGACATGCTGAGTTTGAGGCAAGTGATGTTATCGTAAAGGTTATTTGTTTGCTTCCAAACAGTGTTCATCAAGTTTTTGAAGATTACATTTGGGAAAACTCTAATACTTTACAGGCCAGTGTTTACATCAGAGGAAGTCAAGGTCCAACGCTCCACCCACCAATTCATCAAGAAGGATTTTGTGGGTGTTCAGAAAGCAGAGAGTTCAGTGAAAGAGCCCGGTTTCAATCCCAGTGGCTGCTCTTGACCTTGATAATGAAGAAGAAAAGAAAACAGGAGAAGATGAGAAGGGTGAAAGGTCTGGTGCTTCGTGCTCAAGCTCGGATCTTCCTTGCATGGATAAGCTCAGGGATCAGCTTTCTTGAGAGTGATTGGATCGACCAGTTAAGGAATATGATGGCTGACGGCGGAGAGGGATCTGGTGGCGATATTCAAGGCGTGGAGGAGGAGAAGAAGAAGGAGGCAGAGGTCAAGGCCAAGGAGGAGGCCAAGGTGGTGGAGGAGAAGAAGGACAATGAGGAGGAGAAGAAGAATAAGGAGGCAGAGGTCAAGGCCAAGGAGGAGGCCAAGGTGGTGGAGGAGAAGAAGGACAAGGAGGAGGAGAAGAGTGGAAAGAGAGTTCCAAACAAAGGGAATGGGCTTGATATGGAGAAATACTCATGGACCCAGACAAAAGATATTCTATCAAGTGTAGGGAAGAAAACGGTGGAGTACACGGTGCCATTAGCAGAGAAAACTAAAGACATGGCCGTATTTGCAGGTTGGACTGCAGCGCACGACGGAACTGAGGCACCAGTGGAGGGAACCAAAGCAGCTGCAAGATTAGTTGCCGGAGCAGCAGGGTATGCTGGTCATGAGGCTGTGGATATTGTGTCCAAGCCTTTTGAGTGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

36.35

Weight (kDa)

5.71

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 827
AccB1I GGYRCC 2 cut(s) 796, 868
AciI CCGC 1 cut(s) 486
AclWI GGATC 4 cut(s) 410, 437, 461, 502
AcoI YGGCCR 1 cut(s) 825
AfaI GTAC 2 cut(s) 72, 791
AfiI CCNNNNNNNGG 2 cut(s) 707, 878
AgsI TTSAA 4 cut(s) 49, 158, 305, 512
AloI GAACNNNNNNTCC 2 cut(s) 681, 713
AluBI AGCT 5 cut(s) 12, 398, 423, 435, 893
AluI AGCT 5 cut(s) 12, 398, 423, 435, 893
Alw21I GWGCWC 1 cut(s) 394
AlwI GGATC 4 cut(s) 410, 437, 461, 502
AlwNI CAGNNNCTG 1 cut(s) 316
AoxI GGCC 6 cut(s) 192, 552, 564, 630, 642, 825
ApeKI GCWGC 5 cut(s) 316, 848, 890, 893, 914
AspLEI GCGC 1 cut(s) 853
AspS9I GGNCC 2 cut(s) 221, 741
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 2 cut(s) 221, 741
BanI GGYRCC 2 cut(s) 796, 868
BanII GRGCYC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 5 cut(s) 303, 860, 880, 902, 926
BccI CCATC 2 cut(s) 31, 469
BceAI ACGGC 2 cut(s) 499, 812
BcnI CCSGG 1 cut(s) 298
BfmI CTRYAG 1 cut(s) 846
BfuAI ACCTGC 1 cut(s) 827
BglII AGATCT 1 cut(s) 42
BisI GCNGC 5 cut(s) 317, 849, 891, 894, 915
BlsI GCNGC 5 cut(s) 318, 850, 892, 895, 916
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 2 cut(s) 221, 741
BmgT120I GGNCC 2 cut(s) 221, 741
BmiI GGNNCC 4 cut(s) 743, 798, 870, 883
BmrFI CCNGG 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 719, 751
Bpu10I CCTNAGC 1 cut(s) 424
BpuEI CTTGAG 2 cut(s) 378, 461
BpuMI CCSGG 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 41
BsaJI CCNNGG 4 cut(s) 555, 567, 633, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 707, 878
Bse1I ACTGG 4 cut(s) 195, 310, 460, 872
Bse8I GATNNNNATC 1 cut(s) 41
BseDI CCNNGG 4 cut(s) 555, 567, 633, 645
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 707, 878
BseMII CTCAG 3 cut(s) 78, 438, 855
BseNI ACTGG 4 cut(s) 195, 310, 460, 872
BseXI GCAGC 5 cut(s) 303, 860, 880, 902, 926
BshFI GGCC 6 cut(s) 194, 554, 566, 632, 644, 827
BshNI GGYRCC 2 cut(s) 796, 868
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 2 cut(s) 298, 909
BslI CCNNNNNNNGG 2 cut(s) 707, 878
BsnI GGCC 6 cut(s) 194, 554, 566, 632, 644, 827
Bsp1286I GDGCHC 2 cut(s) 298, 394
Bsp143I GATC 5 cut(s) 42, 402, 429, 453, 494
BspACI CCGC 1 cut(s) 486
BspANI GGCC 6 cut(s) 194, 554, 566, 632, 644, 827
BspCNI CTCAG 3 cut(s) 79, 437, 856
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 4 cut(s) 743, 798, 870, 883
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 4 cut(s) 410, 437, 461, 502
BspT107I GGYRCC 2 cut(s) 796, 868
BsrI ACTGG 4 cut(s) 195, 310, 460, 872
BssECI CCNNGG 4 cut(s) 555, 567, 633, 645
BssMI GATC 5 cut(s) 42, 402, 429, 453, 494
BssT1I CCWWGG 4 cut(s) 555, 567, 633, 645
Bst4CI ACNGT 3 cut(s) 140, 784, 796
Bst6I CTCTTC 1 cut(s) 677
BstAPI GCANNNNNTGC 1 cut(s) 923
BstDEI CTNAG 3 cut(s) 87, 424, 864
BstHHI GCGC 1 cut(s) 853
BstKTI GATC 5 cut(s) 45, 405, 432, 456, 497
BstMBI GATC 5 cut(s) 42, 402, 429, 453, 494
BstMWI GCNNNNNNNGC 3 cut(s) 833, 914, 923
BstNSI RCATGY 2 cut(s) 63, 86
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 846
BstV1I GCAGC 5 cut(s) 303, 860, 880, 902, 926
BstX2I RGATCY 3 cut(s) 42, 402, 494
BstXI CCANNNNNNTGG 2 cut(s) 574, 652
BstYI RGATCY 3 cut(s) 42, 402, 494
BsuRI GGCC 6 cut(s) 194, 554, 566, 632, 644, 827
BtsIMutI CAGTG 6 cut(s) 61, 145, 202, 292, 317, 879
BveI ACCTGC 1 cut(s) 827
CaiI CAGNNNCTG 1 cut(s) 316
CciI TCATGA 1 cut(s) 931
CfoI GCGC 1 cut(s) 853
Cfr13I GGNCC 2 cut(s) 221, 741
Csp6I GTAC 2 cut(s) 71, 790
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
CviAII CATG 7 cut(s) 60, 77, 83, 414, 738, 823, 932
CviQI GTAC 2 cut(s) 71, 790
DdeI CTNAG 3 cut(s) 87, 424, 864
DpnI GATC 5 cut(s) 44, 404, 431, 455, 496
DpnII GATC 5 cut(s) 42, 402, 429, 453, 494
EaeI YGGCCR 1 cut(s) 825
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
EciI GGCGGA 1 cut(s) 501
Eco130I CCWWGG 4 cut(s) 555, 567, 633, 645
Eco24I GRGCYC 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 221, 741
EcoT14I CCWWGG 4 cut(s) 555, 567, 633, 645
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 4 cut(s) 555, 567, 633, 645
FaeI CATG 7 cut(s) 63, 80, 86, 417, 741, 826, 935
FatI CATG 7 cut(s) 59, 76, 82, 413, 737, 822, 931
Fnu4HI GCNGC 5 cut(s) 317, 849, 891, 894, 915
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 5 cut(s) 317, 849, 891, 894, 915
GlaI GCGC 1 cut(s) 852
GluI GCNGC 5 cut(s) 317, 849, 891, 894, 915
HaeIII GGCC 6 cut(s) 194, 554, 566, 632, 644, 827
HapII CCGG 2 cut(s) 298, 909
HhaI GCGC 1 cut(s) 853
Hin1II CATG 7 cut(s) 63, 80, 86, 417, 741, 826, 935
Hin6I GCGC 1 cut(s) 851
HinP1I GCGC 1 cut(s) 851
HpaII CCGG 2 cut(s) 298, 909
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 2 cut(s) 202, 792
Hpy188I TCNGA 3 cut(s) 209, 271, 402
Hpy188III TCNNGA 4 cut(s) 248, 322, 440, 932
Hpy8I GTNNAC 2 cut(s) 202, 792
Hpy99I CGWCG 1 cut(s) 860
HpyAV CCTTC 5 cut(s) 245, 360, 529, 580, 658
HpyCH4III ACNGT 3 cut(s) 140, 784, 796
HpyCH4V TGCA 6 cut(s) 59, 76, 413, 836, 848, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 833, 914, 923
HpyF3I CTNAG 3 cut(s) 87, 424, 864
Hsp92II CATG 7 cut(s) 63, 80, 86, 417, 741, 826, 935
HspAI GCGC 1 cut(s) 851
Kzo9I GATC 5 cut(s) 42, 402, 429, 453, 494
LmnI GCTCC 2 cut(s) 234, 911
Lsp1109I GCAGC 5 cut(s) 303, 860, 880, 902, 926
MalI GATC 5 cut(s) 44, 404, 431, 455, 496
MboI GATC 5 cut(s) 42, 402, 429, 453, 494
MflI RGATCY 3 cut(s) 42, 402, 494
MhlI GDGCHC 2 cut(s) 298, 394
MluCI AATT 1 cut(s) 240
MmeI TCCRAC 2 cut(s) 248, 821
MseI TTAA 1 cut(s) 464
MslI CAYNNNNRTG 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 2 cut(s) 298, 909
MspR9I CCNGG 1 cut(s) 298
MwoI GCNNNNNNNGC 3 cut(s) 833, 914, 923
NciI CCSGG 1 cut(s) 298
NdeII GATC 5 cut(s) 42, 402, 429, 453, 494
NlaIII CATG 7 cut(s) 63, 80, 86, 417, 741, 826, 935
NlaIV GGNNCC 4 cut(s) 743, 798, 870, 883
NspI RCATGY 2 cut(s) 63, 86
PagI TCATGA 1 cut(s) 931
PkrI GCNGC 5 cut(s) 318, 850, 892, 895, 916
PspN4I GGNNCC 4 cut(s) 743, 798, 870, 883
PspPI GGNCC 2 cut(s) 221, 741
PstI CTGCAG 1 cut(s) 850
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 3 cut(s) 42, 402, 494
PvuII CAGCTG 1 cut(s) 893
RsaI GTAC 2 cut(s) 72, 791
RsaNI GTAC 2 cut(s) 71, 790
RseI CAYNNNNRTG 1 cut(s) 81
SaqAI TTAA 1 cut(s) 464
SatI GCNGC 5 cut(s) 317, 849, 891, 894, 915
Sau3AI GATC 5 cut(s) 42, 402, 429, 453, 494
Sau96I GGNCC 2 cut(s) 221, 741
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 2 cut(s) 298, 394
SfcI CTRYAG 1 cut(s) 846
SinI GGWCC 2 cut(s) 221, 741
SmiMI CAYNNNNRTG 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 393, 440
SmoI CTYRAG 2 cut(s) 393, 440
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 296
StyI CCWWGG 4 cut(s) 555, 567, 633, 645
TaaI ACNGT 3 cut(s) 140, 784, 796
TaqI TCGA 1 cut(s) 456
TasI AATT 1 cut(s) 240
TatI WGTACW 2 cut(s) 70, 789
Tru1I TTAA 1 cut(s) 464
Tru9I TTAA 1 cut(s) 464
TscAI CASTG 6 cut(s) 61, 145, 202, 292, 317, 879
TseI GCWGC 5 cut(s) 316, 848, 890, 893, 914
TspDTI ATGAA 3 cut(s) 134, 233, 350
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 6 cut(s) 61, 145, 202, 292, 317, 879
VpaK11BI GGWCC 2 cut(s) 221, 741
XceI RCATGY 2 cut(s) 63, 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.