FvH4_4g14350

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000328
Physical Location & Seq
Forward (+)
17968336 .. 17970547
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14350.t1

Sequence Viewer

Length: 435 bp
ATGTCACCGGAGGAGTGCACCACCAGCGCCATGCATCGACCACCTCACAGTGCCACCGACCTCAGTTTCTCCTTCGTCGATTCTTCAATTTCATGGAGGAGGGAAACTGCATGCAATGTTGCTCCCTTGATCCTCACTGTTGCTGCAAGCACTTTGGATGGAGAATTCCATTCAAACGTACGTATGCTGGAGAGCAAAGGGAGAATCTATCCAGCTGCGGTGTTGTTCAAAAGGGAGCAGCTGCAACTTGGTCATGATCTAATCATAAGCAAAACTGATATAAAGACCTTGAAGGTGAGGCTTGAGGGGATTAAGAAGAAGAGGAACTTTGTGCTCAAGTATTTGAAGAATGATGTTGCTGATCTTCTCAAGAATGGCCTTGACATTAATGCATATGGAAGGGAATATGCTCCTTCTCAACCTTGCAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

145

Amino Acids

16.15

Weight (kDa)

9.19

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 218
AclWI GGATC 1 cut(s) 124
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 1 cut(s) 180
AgsI TTSAA 5 cut(s) 87, 174, 229, 292, 346
AluBI AGCT 3 cut(s) 215, 241, 432
AluI AGCT 3 cut(s) 215, 241, 432
Alw21I GWGCWC 2 cut(s) 20, 336
Alw44I GTGCAC 1 cut(s) 16
AlwI GGATC 1 cut(s) 124
AoxI GGCC 1 cut(s) 376
ApaLI GTGCAC 1 cut(s) 16
ApeKI GCWGC 4 cut(s) 143, 215, 238, 241
ApoI RAATTY 1 cut(s) 164
AseI ATTAAT 1 cut(s) 387
AspLEI GCGC 1 cut(s) 29
AsuHPI GGTGA 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 20
Bbv12I GWGCWC 2 cut(s) 20, 336
BbvI GCAGC 4 cut(s) 130, 202, 228, 250
BccI CCATC 1 cut(s) 152
BfoI RGCGCY 1 cut(s) 30
BisI GCNGC 4 cut(s) 144, 216, 239, 242
BlsI GCNGC 4 cut(s) 145, 217, 240, 243
BmsI GCATC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 3 cut(s) 320, 323, 353
BsaAI YACGTR 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 7
Bse3DI GCAATG 1 cut(s) 121
BseGI GGATG 1 cut(s) 163
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 1 cut(s) 76
BseRI GAGGAG 2 cut(s) 26, 112
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 4 cut(s) 130, 202, 228, 250
BshFI GGCC 1 cut(s) 378
BsiHKAI GWGCWC 2 cut(s) 20, 336
BsiSI CCGG 1 cut(s) 8
BsiWI CGTACG 1 cut(s) 178
BsnI GGCC 1 cut(s) 378
Bsp1286I GDGCHC 2 cut(s) 20, 336
Bsp143I GATC 3 cut(s) 129, 256, 361
BspACI CCGC 1 cut(s) 218
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 253
BspPI GGATC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 121
BssMI GATC 3 cut(s) 129, 256, 361
Bst4CI ACNGT 2 cut(s) 50, 139
Bst6I CTCTTC 1 cut(s) 314
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 112, 148
BstDEI CTNAG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 3 cut(s) 132, 259, 364
BstMBI GATC 3 cut(s) 129, 256, 361
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 20
BstSNI TACGTA 1 cut(s) 182
BstV1I GCAGC 4 cut(s) 130, 202, 228, 250
BsuRI GGCC 1 cut(s) 378
BtsCI GGATG 1 cut(s) 163
BtsIMutI CAGTG 2 cut(s) 55, 135
Cac8I GCNNGC 2 cut(s) 112, 148
CciI TCATGA 1 cut(s) 253
CfoI GCGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 179
CviAII CATG 4 cut(s) 31, 93, 111, 254
CviJI RGCY 5 cut(s) 215, 241, 301, 378, 432
CviKI_1 RGCY 5 cut(s) 215, 241, 301, 378, 432
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 1 cut(s) 62
DpnI GATC 3 cut(s) 131, 258, 363
DpnII GATC 3 cut(s) 129, 256, 361
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
Eco105I TACGTA 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 164
EcoT22I ATGCAT 2 cut(s) 36, 394
FaeI CATG 4 cut(s) 34, 96, 114, 257
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FatI CATG 4 cut(s) 30, 92, 110, 253
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 4 cut(s) 144, 216, 239, 242
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 144, 216, 239, 242
GlaI GCGC 1 cut(s) 28
GluI GCNGC 4 cut(s) 144, 216, 239, 242
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 1 cut(s) 378
HapII CCGG 1 cut(s) 8
HhaI GCGC 1 cut(s) 29
Hin1II CATG 4 cut(s) 34, 96, 114, 257
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 2 cut(s) 80, 204
HpaII CCGG 1 cut(s) 8
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 18
Hpy188III TCNNGA 2 cut(s) 254, 370
Hpy8I GTNNAC 1 cut(s) 18
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 4 cut(s) 82, 286, 393, 423
HpyCH4III ACNGT 2 cut(s) 50, 139
HpyCH4IV ACGT 2 cut(s) 177, 181
HpyCH4V TGCA 8 cut(s) 18, 34, 110, 114, 146, 244, 392, 426
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 62
HpySE526I ACGT 2 cut(s) 177, 181
Hsp92II CATG 4 cut(s) 34, 96, 114, 257
HspAI GCGC 1 cut(s) 27
Kzo9I GATC 3 cut(s) 129, 256, 361
LmnI GCTCC 3 cut(s) 127, 235, 415
LpnPI CCDG 4 cut(s) 21, 37, 173, 225
Lsp1109I GCAGC 4 cut(s) 130, 202, 228, 250
LweI GCATC 1 cut(s) 43
MaeII ACGT 2 cut(s) 177, 181
MaeIII GTNAC 1 cut(s) 3
MalI GATC 3 cut(s) 131, 258, 363
MboI GATC 3 cut(s) 129, 256, 361
MboII GAAGA 5 cut(s) 75, 328, 331, 356, 358
MhlI GDGCHC 2 cut(s) 20, 336
MluCI AATT 2 cut(s) 87, 164
MnlI CCTC 9 cut(s) 4, 54, 71, 90, 93, 143, 291, 298, 315
Mph1103I ATGCAT 2 cut(s) 36, 394
MseI TTAA 2 cut(s) 312, 387
MspA1I CMGCKG 2 cut(s) 215, 241
MspI CCGG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 24
NdeI CATATG 1 cut(s) 394
NdeII GATC 3 cut(s) 129, 256, 361
NlaIII CATG 4 cut(s) 34, 96, 114, 257
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 2 cut(s) 36, 394
NspI RCATGY 1 cut(s) 114
PaeI GCATGC 1 cut(s) 114
PagI TCATGA 1 cut(s) 253
PfeI GAWTC 2 cut(s) 80, 204
Pfl23II CGTACG 1 cut(s) 178
PkrI GCNGC 4 cut(s) 145, 217, 240, 243
Ppu21I YACGTR 1 cut(s) 182
PshBI ATTAAT 1 cut(s) 387
PspLI CGTACG 1 cut(s) 178
PvuII CAGCTG 2 cut(s) 215, 241
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SaqAI TTAA 2 cut(s) 312, 387
SatI GCNGC 4 cut(s) 144, 216, 239, 242
Sau3AI GATC 3 cut(s) 129, 256, 361
SduI GDGCHC 2 cut(s) 20, 336
SfaNI GCATC 1 cut(s) 43
SmlI CTYRAG 3 cut(s) 302, 335, 368
SmoI CTYRAG 3 cut(s) 302, 335, 368
SnaBI TACGTA 1 cut(s) 182
SphI GCATGC 1 cut(s) 114
Sse9I AATT 2 cut(s) 87, 164
SsiI CCGC 1 cut(s) 218
TaaI ACNGT 2 cut(s) 50, 139
TaiI ACGT 2 cut(s) 180, 184
TaqI TCGA 2 cut(s) 37, 78
TasI AATT 2 cut(s) 87, 164
TfiI GAWTC 2 cut(s) 80, 204
Tru1I TTAA 2 cut(s) 312, 387
Tru9I TTAA 2 cut(s) 312, 387
TscAI CASTG 2 cut(s) 55, 142
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 143, 215, 238, 241
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 81
TspRI CASTG 2 cut(s) 55, 142
VneI GTGCAC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 1 cut(s) 164
XceI RCATGY 1 cut(s) 114
Zsp2I ATGCAT 2 cut(s) 36, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.