FvH4_3g26701

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000328
Physical Location & Seq
Reverse (-)
19825671 .. 19827263
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g26701.t1

Sequence Viewer

Length: 435 bp
ATGGAGGAGGTGAGTGAGGTTGAGAAGGCTGAACCTTGGTTCGACTCCGGTCTCACCTACGCCTCAACTTCTTCAGTGCATGTATTTTTCAATCTCCCAAATCCTTTTCGCTGGGAAACTGCATGCAATGTTGCTCCCTTGATCCTCACTGTTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACGTACGTATGCTGGAGAGCAAAGGGAGAATCTATCCAGCTGCGGTGTTGTTCAAAAGGGAGCAGCTGCAACTTGGTCATGATCTGATCATAAGCAAAACTGATATAAAGACCTTGAAGGTGAGGCTTGAGGGGATTAAGAAAAAGAGGAACTTTGTGCTCAAGTATTTGAAGAATGATGTTGTTGATCTTCTCAAGAATGGCCTTGACATTAATGCATATGGAAGGGTAAGTTTTATTGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

145

Amino Acids

16.45

Weight (kDa)

9.01

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 95 - 143 8.4e-06 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 230
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 1 cut(s) 192
AgsI TTSAA 5 cut(s) 91, 186, 241, 304, 358
AluBI AGCT 2 cut(s) 227, 253
AluI AGCT 2 cut(s) 227, 253
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 136
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 4 cut(s) 155, 227, 250, 253
ApoI RAATTY 1 cut(s) 176
AseI ATTAAT 1 cut(s) 399
AsuHPI GGTGA 3 cut(s) 22, 46, 319
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 4 cut(s) 142, 214, 240, 262
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 56
BisI GCNGC 4 cut(s) 156, 228, 251, 254
BlsI GCNGC 4 cut(s) 157, 229, 252, 255
BoxI GACNNNNGTC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 221
BpuEI CTTGAG 3 cut(s) 332, 335, 365
BsaAI YACGTR 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 35
BsaWI WCCGGW 1 cut(s) 47
Bse3DI GCAATG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 133
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 142, 214, 240, 262
BseYI CCCAGC 1 cut(s) 111
BshFI GGCC 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 1 cut(s) 48
BsiWI CGTACG 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 56
BsnI GGCC 1 cut(s) 390
Bso31I GGTCTC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 4 cut(s) 141, 268, 273, 373
BspACI CCGC 1 cut(s) 230
BspANI GGCC 1 cut(s) 390
BspHI TCATGA 1 cut(s) 265
BspPI GGATC 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 4 cut(s) 141, 268, 273, 373
BssT1I CCWWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 151
BstBAI YACGTR 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 124, 160
BstKTI GATC 4 cut(s) 144, 271, 276, 376
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 4 cut(s) 141, 268, 273, 373
BstNSI RCATGY 2 cut(s) 83, 126
BstPAI GACNNNNGTC 1 cut(s) 48
BstSNI TACGTA 1 cut(s) 194
BstV1I GCAGC 4 cut(s) 142, 214, 240, 262
BsuRI GGCC 1 cut(s) 390
BtsIMutI CAGTG 2 cut(s) 81, 147
Cac8I GCNNGC 2 cut(s) 124, 160
CciI TCATGA 1 cut(s) 265
Csp6I GTAC 1 cut(s) 191
CviAII CATG 3 cut(s) 80, 123, 266
CviJI RGCY 5 cut(s) 29, 227, 253, 313, 390
CviKI_1 RGCY 5 cut(s) 29, 227, 253, 313, 390
CviQI GTAC 1 cut(s) 191
DpnI GATC 4 cut(s) 143, 270, 275, 375
DpnII GATC 4 cut(s) 141, 268, 273, 373
Eco105I TACGTA 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 57
EcoRI GAATTC 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 35
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 35
FaeI CATG 3 cut(s) 83, 126, 269
FaiI YATR 8 cut(s) 81, 124, 197, 267, 278, 293, 406, 408
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 3 cut(s) 79, 122, 265
FauNDI CATATG 1 cut(s) 406
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 4 cut(s) 156, 228, 251, 254
Fsp4HI GCNGC 4 cut(s) 156, 228, 251, 254
GluI GCNGC 4 cut(s) 156, 228, 251, 254
GsaI CCCAGC 1 cut(s) 115
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 390
HapII CCGG 1 cut(s) 48
Hin1II CATG 3 cut(s) 83, 126, 269
HinfI GANTC 2 cut(s) 44, 216
HpaII CCGG 1 cut(s) 48
HphI GGTGA 3 cut(s) 22, 46, 319
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 266, 382
HpyAV CCTTC 3 cut(s) 19, 298, 405
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 2 cut(s) 189, 193
HpyCH4V TGCA 6 cut(s) 79, 122, 126, 158, 256, 404
HpySE526I ACGT 2 cut(s) 189, 193
Hsp92II CATG 3 cut(s) 83, 126, 269
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 4 cut(s) 141, 268, 273, 373
LmnI GCTCC 2 cut(s) 139, 247
LpnPI CCDG 4 cut(s) 61, 97, 185, 237
Lsp1109I GCAGC 4 cut(s) 142, 214, 240, 262
MaeII ACGT 2 cut(s) 189, 193
MalI GATC 4 cut(s) 143, 270, 275, 375
MboI GATC 4 cut(s) 141, 268, 273, 373
MboII GAAGA 3 cut(s) 63, 368, 370
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 1 cut(s) 176
MlyI GAGTC 1 cut(s) 38
MnlI CCTC 6 cut(s) 10, 73, 155, 303, 310, 327
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 2 cut(s) 324, 399
MspA1I CMGCKG 2 cut(s) 227, 253
MspI CCGG 1 cut(s) 48
NdeI CATATG 1 cut(s) 406
NdeII GATC 4 cut(s) 141, 268, 273, 373
NlaIII CATG 3 cut(s) 83, 126, 269
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 2 cut(s) 83, 126
PaeI GCATGC 1 cut(s) 126
PagI TCATGA 1 cut(s) 265
PfeI GAWTC 1 cut(s) 216
Pfl23II CGTACG 1 cut(s) 190
PkrI GCNGC 4 cut(s) 157, 229, 252, 255
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Ppu21I YACGTR 1 cut(s) 194
PshAI GACNNNNGTC 1 cut(s) 48
PshBI ATTAAT 1 cut(s) 399
PspFI CCCAGC 1 cut(s) 111
PspLI CGTACG 1 cut(s) 190
PvuII CAGCTG 2 cut(s) 227, 253
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 324, 399
SatI GCNGC 4 cut(s) 156, 228, 251, 254
Sau3AI GATC 4 cut(s) 141, 268, 273, 373
SchI GAGTC 1 cut(s) 38
SduI GDGCHC 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 314, 347, 380
SmoI CTYRAG 3 cut(s) 314, 347, 380
SnaBI TACGTA 1 cut(s) 194
SphI GCATGC 1 cut(s) 126
Sse9I AATT 1 cut(s) 176
SsiI CCGC 1 cut(s) 230
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 2 cut(s) 192, 196
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 176
TfiI GAWTC 1 cut(s) 216
Tru1I TTAA 2 cut(s) 324, 399
Tru9I TTAA 2 cut(s) 324, 399
TscAI CASTG 2 cut(s) 81, 154
TseI GCWGC 4 cut(s) 155, 227, 250, 253
TspDTI ATGAA 1 cut(s) 419
TspRI CASTG 2 cut(s) 81, 154
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 1 cut(s) 176
XceI RCATGY 2 cut(s) 83, 126
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.