RLG00000024515

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000328
Physical Location & Seq
Reverse (-)
36621055 .. 36622098
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024515

Sequence Viewer

Length: 351 bp
ATGGATAACAATGATGATCTTGCTGGAGATGCCATTGAAAGTGATGACAAGGAAGAACAGTATGAAATTATATTGATTGGTTTTGGCCAACCCTTTACCAAATCTAATTTTTGGATGACCAGCATCAGTGCCCCTATGATGGTTGAGGTGGAGGGTGAGGCTGACCCACTTGAGAAACATGTGAGAGGTCCAATTGCCTTGTCTGATATAAAGAGCATAAAGGTGAGGCTAGGGGTGATTAAGAAGAAGAGGAACGCTGTGCTGAAGTTTCTGAAGAATAACGTTGCTGTTCTTCTCAAGATTGGCCTTGATATTAATGTATATGGAGGGTTAAGTTTCATGGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

117

Amino Acids

12.86

Weight (kDa)

5.33

Isoelectric Point (pI)

21.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 282
AcoI YGGCCR 1 cut(s) 85
AcuI CTGAAG 2 cut(s) 284, 293
AfiI CCNNNNNNNGG 1 cut(s) 139
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 1 cut(s) 38
AoxI GGCC 2 cut(s) 85, 304
AseI ATTAAT 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 3 cut(s) 167, 235, 247
AvaII GGWCC 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 133
BalI TGGCCA 1 cut(s) 87
BccI CCATC 1 cut(s) 133
BfaI CTAG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 188
BmsI GCATC 2 cut(s) 19, 132
BpmI CTGGAG 1 cut(s) 45
BpuEI CTTGAG 2 cut(s) 191, 281
Bsc4I CCNNNNNNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 133
BshFI GGCC 2 cut(s) 87, 306
BslI CCNNNNNNNGG 1 cut(s) 139
BsnI GGCC 2 cut(s) 87, 306
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 16
BspANI GGCC 2 cut(s) 87, 306
BssMI GATC 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 60
Bst6I CTCTTC 1 cut(s) 242
BstF5I GGATG 1 cut(s) 120
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 182
BstSLI GKGCMC 1 cut(s) 133
BsuRI GGCC 2 cut(s) 87, 306
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 188
CviAII CATG 2 cut(s) 179, 340
CviJI RGCY 5 cut(s) 87, 161, 229, 306, 344
CviKI_1 RGCY 5 cut(s) 87, 161, 229, 306, 344
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 284, 293
FaeI CATG 2 cut(s) 182, 343
FaiI YATR 9 cut(s) 63, 71, 137, 180, 209, 218, 322, 324, 341
FatI CATG 2 cut(s) 178, 339
FokI GGATG 1 cut(s) 127
FspBI CTAG 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 2 cut(s) 87, 306
Hin1II CATG 2 cut(s) 182, 343
HphI GGTGA 3 cut(s) 167, 235, 247
Hpy188I TCNGA 2 cut(s) 205, 273
Hpy188III TCNNGA 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 2 cut(s) 182, 343
Kzo9I GATC 1 cut(s) 16
LpnPI CCDG 2 cut(s) 9, 133
LweI GCATC 2 cut(s) 19, 132
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 282
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 5 cut(s) 65, 256, 259, 284, 286
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 3 cut(s) 66, 106, 192
MluNI TGGCCA 1 cut(s) 87
MnlI CCTC 7 cut(s) 139, 145, 151, 179, 219, 243, 320
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
MseI TTAA 3 cut(s) 240, 315, 332
MslI CAYNNNNRTG 1 cut(s) 221
Msp20I TGGCCA 1 cut(s) 87
MunI CAATTG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 29
NdeII GATC 1 cut(s) 16
NlaIII CATG 2 cut(s) 182, 343
NspI RCATGY 1 cut(s) 182
PciI ACATGT 1 cut(s) 178
PscI ACATGT 1 cut(s) 178
PshBI ATTAAT 1 cut(s) 315
Psp1406I AACGTT 1 cut(s) 282
PspPI GGNCC 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 3 cut(s) 240, 315, 332
Sau3AI GATC 1 cut(s) 16
Sau96I GGNCC 1 cut(s) 188
SduI GDGCHC 1 cut(s) 133
SetI ASST 4 cut(s) 150, 190, 225, 285
SfaNI GCATC 2 cut(s) 19, 132
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 170, 296
SmoI CTYRAG 2 cut(s) 170, 296
Sse9I AATT 3 cut(s) 66, 106, 192
SspMI CTAG 1 cut(s) 230
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 1 cut(s) 285
TasI AATT 3 cut(s) 66, 106, 192
Tru1I TTAA 3 cut(s) 240, 315, 332
Tru9I TTAA 3 cut(s) 240, 315, 332
TscAI CASTG 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 78, 328
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 315
XceI RCATGY 1 cut(s) 182
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.