MD14G1070200.v1.1

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000328
Physical Location & Seq
Forward (+)
7723860 .. 7725234
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1070200.v1.1.491

Sequence Viewer

Length: 450 bp
ATGACGGTCACAGCGCAGATTTCGATCAATGATGGGAACCTGGTGGTTCATGGAAAGACCATTTTGACTGGGGTTCCAGACAACATCATACTGACACCCGGGACCGGTGTCGGACTCCTTGCCGGTGCTTTCATTGGCGCCACGGCCGCCCACAACAAAAGCCTCCATATCTTCCCCATTGGGGTTTTAGAGGATCTCCGTTTCATGTGCTGTTTTCGATTCAAGTTGTGGTGGATGACTCAGAGGATGGGGACATGTGGGAAGGATGTTCCATTGGAGACCCAATTCATGGTGGTGGAGAGCAAAGGTGGTGGTGATGGAGGTGAAGACGACGAGTCTTCTCCTATCATCTACACCGTGTTCCTGCCTCTACTCGAGGGCCCTTTCCGCTCTGTTCTGCAAGGGAATGAGAGGAACGAAGTCGAGGTTTGCCTCGAGAGTGGTGAGTAG

Protein Analysis

150

Amino Acids

16.09

Weight (kDa)

4.77

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 149 6.2e-53 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 289
AccBSI CCGCTC 1 cut(s) 390
AciI CCGC 2 cut(s) 147, 388
AclWI GGATC 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 138
AfiI CCNNNNNNNGG 3 cut(s) 104, 181, 289
AflIII ACRYGT 1 cut(s) 254
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 1 cut(s) 223
AhdI GACNNNNNGTC 1 cut(s) 334
AjnI CCWGG 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 1 cut(s) 201
Ama87I CYCGRG 3 cut(s) 98, 374, 434
AoxI GGCC 2 cut(s) 144, 379
ApaI GGGCCC 1 cut(s) 383
AsiGI ACCGGT 1 cut(s) 104
AspLEI GCGC 2 cut(s) 16, 140
AspS9I GGNCC 3 cut(s) 102, 379, 380
AsuC2I CCSGG 2 cut(s) 99, 100
AsuHPI GGTGA 2 cut(s) 326, 335
AvaI CYCGRG 3 cut(s) 98, 374, 434
AvaII GGWCC 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 383
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 2 cut(s) 330, 333
BccI CCATC 3 cut(s) 26, 241, 311
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 41
BcnI CCSGG 2 cut(s) 99, 100
BcoDI GTCTC 1 cut(s) 272
BfoI RGCGCY 1 cut(s) 141
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 3 cut(s) 41, 99, 100
Bme18I GGWCC 1 cut(s) 102
BmeRI GACNNNNNGTC 1 cut(s) 334
BmeT110I CYCGRG 3 cut(s) 98, 374, 434
BmgT120I GGNCC 3 cut(s) 102, 379, 380
BmiI GGNNCC 5 cut(s) 38, 75, 103, 139, 381
BmrFI CCNGG 3 cut(s) 41, 99, 100
BmrI ACTGGG 1 cut(s) 78
BmuI ACTGGG 1 cut(s) 78
BoxI GACNNNNGTC 1 cut(s) 107
BpiI GAAGAC 2 cut(s) 330, 333
BpuMI CCSGG 2 cut(s) 99, 100
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 98, 141
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 181, 289
Bse118I RCCGGY 2 cut(s) 104, 122
Bse1I ACTGG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 98, 141
BseGI GGATG 3 cut(s) 240, 252, 271
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 3 cut(s) 104, 181, 289
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 383
BseX3I CGGCCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 146, 381
BshNI GGYRCC 1 cut(s) 137
BshTI ACCGGT 1 cut(s) 104
BsiEI CGRYCG 1 cut(s) 147
BsiHKCI CYCGRG 3 cut(s) 98, 374, 434
BsiSI CCGG 3 cut(s) 99, 105, 123
BslFI GGGAC 2 cut(s) 115, 265
BslI CCNNNNNNNGG 3 cut(s) 104, 181, 289
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 2 cut(s) 115, 265
BsnI GGCC 2 cut(s) 146, 381
Bso31I GGTCTC 1 cut(s) 272
BsoBI CYCGRG 3 cut(s) 98, 374, 434
Bsp120I GGGCCC 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 2 cut(s) 24, 193
BspACI CCGC 2 cut(s) 147, 388
BspANI GGCC 2 cut(s) 146, 381
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 5 cut(s) 38, 75, 103, 139, 381
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 272
BsrBI CCGCTC 1 cut(s) 390
BsrFI RCCGGY 2 cut(s) 104, 122
BsrI ACTGG 1 cut(s) 73
BssAI RCCGGY 2 cut(s) 104, 122
BssECI CCNNGG 2 cut(s) 98, 141
BssMI GATC 2 cut(s) 24, 193
BssNI GRCGYC 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 7, 358
BstACI GRCGYC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 240, 252, 271
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 2 cut(s) 16, 140
BstKTI GATC 2 cut(s) 27, 196
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 24, 193
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 2 cut(s) 146, 387
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 1 cut(s) 258
BstPAI GACNNNNGTC 1 cut(s) 107
BstSCI CCNGG 3 cut(s) 39, 97, 98
BstSLI GKGCMC 1 cut(s) 383
BstV2I GAAGAC 2 cut(s) 330, 333
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BstZI CGGCCG 1 cut(s) 144
BsuRI GGCC 2 cut(s) 146, 381
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 3 cut(s) 240, 252, 271
CfoI GCGC 2 cut(s) 16, 140
Cfr10I RCCGGY 2 cut(s) 104, 122
Cfr13I GGNCC 3 cut(s) 102, 379, 380
Cfr9I CCCGGG 1 cut(s) 98
CsiI ACCWGGT 1 cut(s) 39
CspAI ACCGGT 1 cut(s) 104
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 4 cut(s) 50, 205, 255, 289
CviJI RGCY 3 cut(s) 146, 162, 381
CviKI_1 RGCY 3 cut(s) 146, 162, 381
DdeI CTNAG 1 cut(s) 240
DinI GGCGCC 1 cut(s) 139
DpnI GATC 2 cut(s) 26, 195
DpnII GATC 2 cut(s) 24, 193
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 144
EagI CGGCCG 1 cut(s) 144
Eam1105I GACNNNNNGTC 1 cut(s) 334
EclXI CGGCCG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 102
Eco52I CGGCCG 1 cut(s) 144
Eco88I CYCGRG 3 cut(s) 98, 374, 434
EcoO109I RGGNCCY 2 cut(s) 379, 380
EcoRII CCWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 383
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
FaeI CATG 4 cut(s) 53, 208, 258, 292
FaiI YATR 6 cut(s) 51, 89, 168, 206, 256, 290
FaqI GGGAC 2 cut(s) 115, 265
FatI CATG 4 cut(s) 49, 204, 254, 288
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 3 cut(s) 247, 259, 278
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 1 cut(s) 147
GlaI GCGC 2 cut(s) 15, 139
GluI GCNGC 1 cut(s) 147
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 2 cut(s) 146, 381
HapII CCGG 3 cut(s) 99, 105, 123
HhaI GCGC 2 cut(s) 16, 140
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 4 cut(s) 53, 208, 258, 292
Hin6I GCGC 2 cut(s) 14, 138
HinP1I GCGC 2 cut(s) 14, 138
HinfI GANTC 4 cut(s) 114, 219, 238, 335
HpaII CCGG 3 cut(s) 99, 105, 123
HphI GGTGA 2 cut(s) 326, 335
Hpy188I TCNGA 2 cut(s) 113, 243
Hpy188III TCNNGA 2 cut(s) 77, 436
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 2 cut(s) 7, 358
HpyCH4V TGCA 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 387
HpyF3I CTNAG 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 4 cut(s) 53, 208, 258, 292
HspAI GCGC 2 cut(s) 14, 138
KasI GGCGCC 1 cut(s) 137
Kzo9I GATC 2 cut(s) 24, 193
LpnPI CCDG 8 cut(s) 26, 53, 54, 90, 112, 118, 136, 377
MabI ACCWGGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 7
MalI GATC 2 cut(s) 26, 195
MbiI CCGCTC 1 cut(s) 390
MboI GATC 2 cut(s) 24, 193
MboII GAAGA 3 cut(s) 163, 330, 338
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 1 cut(s) 284
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 3 cut(s) 108, 232, 344
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 9 cut(s) 173, 184, 237, 314, 370, 378, 405, 418, 443
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 3 cut(s) 99, 105, 123
MspR9I CCNGG 3 cut(s) 41, 99, 100
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 146, 387
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 2 cut(s) 99, 100
NdeII GATC 2 cut(s) 24, 193
NlaIII CATG 4 cut(s) 53, 208, 258, 292
NlaIV GGNNCC 5 cut(s) 38, 75, 103, 139, 381
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 258
PaeR7I CTCGAG 2 cut(s) 374, 434
PciI ACATGT 1 cut(s) 254
PfeI GAWTC 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 289
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 3 cut(s) 108, 232, 343
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 3 cut(s) 108, 232, 343
PscI ACATGT 1 cut(s) 254
PshAI GACNNNNGTC 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 5 cut(s) 38, 75, 103, 139, 381
PspOMI GGGCCC 1 cut(s) 379
PspPI GGNCC 3 cut(s) 102, 379, 380
PspXI VCTCGAGB 1 cut(s) 374
PsuI RGATCY 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 293
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 2 cut(s) 24, 193
Sau96I GGNCC 3 cut(s) 102, 379, 380
SchI GAGTC 3 cut(s) 108, 232, 344
ScrFI CCNGG 3 cut(s) 41, 99, 100
SduI GDGCHC 1 cut(s) 383
SetI ASST 4 cut(s) 42, 310, 325, 429
SexAI ACCWGGT 1 cut(s) 39
SfoI GGCGCC 1 cut(s) 139
Sfr274I CTCGAG 2 cut(s) 374, 434
SinI GGWCC 1 cut(s) 102
SlaI CTCGAG 2 cut(s) 374, 434
SmaI CCCGGG 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 2 cut(s) 374, 434
SmoI CTYRAG 2 cut(s) 374, 434
Sse9I AATT 1 cut(s) 284
SsiI CCGC 2 cut(s) 147, 388
SspDI GGCGCC 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 39, 97, 98
TaaI ACNGT 2 cut(s) 7, 358
TaqI TCGA 5 cut(s) 23, 217, 375, 423, 435
TasI AATT 1 cut(s) 284
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 1 cut(s) 219
TseFI GTSAC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 4 cut(s) 38, 121, 193, 277
TspGWI ACGGA 1 cut(s) 188
TspMI CCCGGG 1 cut(s) 98
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 102
XceI RCATGY 1 cut(s) 258
XhoI CTCGAG 2 cut(s) 374, 434
XmaI CCCGGG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.