DNA-directed RNA polymerases I, II, and III subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 702 |
| Acc36I | ACCTGC | 1 cut(s) | 1038 |
| AccB1I | GGYRCC | 1 cut(s) | 83 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 372 |
| AccBSI | CCGCTC | 1 cut(s) | 1000 |
| AciI | CCGC | 2 cut(s) | 876, 1000 |
| AclWI | GGATC | 1 cut(s) | 797 |
| AcoI | YGGCCR | 2 cut(s) | 3, 612 |
| AcsI | RAATTY | 2 cut(s) | 524, 900 |
| AcuI | CTGAAG | 1 cut(s) | 462 |
| AcyI | GRCGYC | 1 cut(s) | 699 |
| AfaI | GTAC | 3 cut(s) | 247, 821, 1085 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 86, 242, 334, 372, 925 |
| AflIII | ACRYGT | 1 cut(s) | 1116 |
| AgsI | TTSAA | 5 cut(s) | 509, 524, 589, 728, 861 |
| AjnI | CCWGG | 1 cut(s) | 79 |
| AluBI | AGCT | 9 cut(s) | 505, 559, 596, 647, 783, 795, 991, 1163, 1182 |
| AluI | AGCT | 9 cut(s) | 505, 559, 596, 647, 783, 795, 991, 1163, 1182 |
| Alw21I | GWGCWC | 1 cut(s) | 465 |
| Alw26I | GTCTC | 2 cut(s) | 938, 965 |
| Alw44I | GTGCAC | 1 cut(s) | 461 |
| AlwI | GGATC | 1 cut(s) | 797 |
| AoxI | GGCC | 3 cut(s) | 3, 612, 882 |
| ApaLI | GTGCAC | 1 cut(s) | 461 |
| ApeKI | GCWGC | 4 cut(s) | 502, 593, 685, 1130 |
| ApoI | RAATTY | 2 cut(s) | 524, 900 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 856 |
| AspLEI | GCGC | 2 cut(s) | 371, 407 |
| AsuHPI | GGTGA | 3 cut(s) | 378, 542, 953 |
| AsuII | TTCGAA | 1 cut(s) | 653 |
| AxyI | CCTNAGG | 1 cut(s) | 111 |
| BaeGI | GKGCMC | 1 cut(s) | 465 |
| BalI | TGGCCA | 1 cut(s) | 614 |
| BanI | GGYRCC | 1 cut(s) | 83 |
| Bbv12I | GWGCWC | 1 cut(s) | 465 |
| BbvI | GCAGC | 4 cut(s) | 514, 605, 672, 1142 |
| BccI | CCATC | 5 cut(s) | 259, 392, 416, 919, 1025 |
| BceAI | ACGGC | 1 cut(s) | 897 |
| BciT130I | CCWGG | 1 cut(s) | 81 |
| BcoDI | GTCTC | 2 cut(s) | 938, 965 |
| BfaI | CTAG | 3 cut(s) | 159, 560, 741 |
| BfmI | CTRYAG | 2 cut(s) | 291, 1128 |
| BfoI | RGCGCY | 1 cut(s) | 408 |
| BfuAI | ACCTGC | 1 cut(s) | 1038 |
| BisI | GCNGC | 4 cut(s) | 503, 594, 686, 1131 |
| BlsI | GCNGC | 4 cut(s) | 504, 595, 687, 1132 |
| Bme1390I | CCNGG | 1 cut(s) | 81 |
| BmiI | GGNNCC | 1 cut(s) | 85 |
| BmrFI | CCNGG | 1 cut(s) | 81 |
| BmrI | ACTGGG | 1 cut(s) | 588 |
| BmuI | ACTGGG | 1 cut(s) | 588 |
| Bpu10I | CCTNAGC | 1 cut(s) | 784 |
| Bpu14I | TTCGAA | 1 cut(s) | 653 |
| BpuEI | CTTGAG | 5 cut(s) | 575, 754, 821, 976, 1095 |
| BsaAI | YACGTR | 1 cut(s) | 541 |
| BsaHI | GRCGYC | 1 cut(s) | 699 |
| BsaJI | CCNNGG | 1 cut(s) | 79 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 86, 242, 334, 372, 925 |
| Bse118I | RCCGGY | 1 cut(s) | 86 |
| Bse1I | ACTGG | 3 cut(s) | 359, 583, 1161 |
| Bse21I | CCTNAGG | 1 cut(s) | 111 |
| Bse3DI | GCAATG | 2 cut(s) | 433, 769 |
| BseBI | CCWGG | 1 cut(s) | 81 |
| BseDI | CCNNGG | 1 cut(s) | 79 |
| BseGI | GGATG | 5 cut(s) | 16, 121, 187, 251, 269 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 86, 242, 334, 372, 925 |
| BseMI | GCAATG | 2 cut(s) | 433, 769 |
| BseMII | CTCAG | 5 cut(s) | 125, 191, 270, 465, 798 |
| BseNI | ACTGG | 3 cut(s) | 359, 583, 1161 |
| BseRI | GAGGAG | 2 cut(s) | 18, 318 |
| BseSI | GKGCMC | 1 cut(s) | 465 |
| BseXI | GCAGC | 4 cut(s) | 514, 605, 672, 1142 |
| BsgI | GTGCAG | 1 cut(s) | 1083 |
| Bsh1285I | CGRYCG | 1 cut(s) | 91 |
| BshFI | GGCC | 3 cut(s) | 5, 614, 884 |
| BshNI | GGYRCC | 1 cut(s) | 83 |
| BsiEI | CGRYCG | 1 cut(s) | 91 |
| BsiHKAI | GWGCWC | 1 cut(s) | 465 |
| BsiSI | CCGG | 1 cut(s) | 87 |
| BslFI | GGGAC | 2 cut(s) | 248, 311 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 86, 242, 334, 372, 925 |
| BsmAI | GTCTC | 2 cut(s) | 938, 965 |
| BsmFI | GGGAC | 2 cut(s) | 248, 311 |
| BsmI | GAATGC | 1 cut(s) | 919 |
| BsnI | GGCC | 3 cut(s) | 5, 614, 884 |
| Bsp119I | TTCGAA | 1 cut(s) | 653 |
| Bsp1286I | GDGCHC | 1 cut(s) | 465 |
| Bsp143I | GATC | 2 cut(s) | 280, 789 |
| BspACI | CCGC | 2 cut(s) | 876, 1000 |
| BspANI | GGCC | 3 cut(s) | 5, 614, 884 |
| BspCNI | CTCAG | 5 cut(s) | 124, 190, 269, 464, 797 |
| BspHI | TCATGA | 1 cut(s) | 495 |
| BspLI | GGNNCC | 1 cut(s) | 85 |
| BspMAI | CTGCAG | 2 cut(s) | 295, 1132 |
| BspMI | ACCTGC | 1 cut(s) | 1038 |
| BspPI | GGATC | 1 cut(s) | 797 |
| BspT104I | TTCGAA | 1 cut(s) | 653 |
| BspT107I | GGYRCC | 1 cut(s) | 83 |
| BsrBI | CCGCTC | 1 cut(s) | 1000 |
| BsrDI | GCAATG | 2 cut(s) | 433, 769 |
| BsrFI | RCCGGY | 1 cut(s) | 86 |
| BsrI | ACTGG | 3 cut(s) | 359, 583, 1161 |
| BssAI | RCCGGY | 1 cut(s) | 86 |
| BssECI | CCNNGG | 1 cut(s) | 79 |
| BssMI | GATC | 2 cut(s) | 280, 789 |
| BssNI | GRCGYC | 1 cut(s) | 699 |
| Bst2UI | CCWGG | 1 cut(s) | 81 |
| Bst4CI | ACNGT | 2 cut(s) | 349, 680 |
| BstACI | GRCGYC | 1 cut(s) | 699 |
| BstBAI | YACGTR | 1 cut(s) | 541 |
| BstBI | TTCGAA | 1 cut(s) | 653 |
| BstC8I | GCNNGC | 1 cut(s) | 1066 |
| BstDEI | CTNAG | 6 cut(s) | 111, 177, 256, 451, 779, 784 |
| BstF5I | GGATG | 5 cut(s) | 16, 121, 187, 251, 269 |
| BstH2I | RGCGCY | 1 cut(s) | 408 |
| BstHHI | GCGC | 2 cut(s) | 371, 407 |
| BstKTI | GATC | 2 cut(s) | 283, 792 |
| BstMAI | GTCTC | 2 cut(s) | 938, 965 |
| BstMBI | GATC | 2 cut(s) | 280, 789 |
| BstMCI | CGRYCG | 1 cut(s) | 91 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 499, 997 |
| BstNI | CCWGG | 1 cut(s) | 81 |
| BstNSI | RCATGY | 2 cut(s) | 830, 1120 |
| BstSCI | CCNGG | 1 cut(s) | 79 |
| BstSFI | CTRYAG | 2 cut(s) | 291, 1128 |
| BstSLI | GKGCMC | 1 cut(s) | 465 |
| BstV1I | GCAGC | 4 cut(s) | 514, 605, 672, 1142 |
| Bsu36I | CCTNAGG | 1 cut(s) | 111 |
| BsuRI | GGCC | 3 cut(s) | 5, 614, 884 |
| BtsCI | GGATG | 5 cut(s) | 16, 121, 187, 251, 269 |
| BveI | ACCTGC | 1 cut(s) | 1038 |
| Cac8I | GCNNGC | 1 cut(s) | 1066 |
| CciI | TCATGA | 1 cut(s) | 495 |
| CfoI | GCGC | 2 cut(s) | 371, 407 |
| Cfr10I | RCCGGY | 1 cut(s) | 86 |
| Csp6I | GTAC | 3 cut(s) | 246, 820, 1084 |
| CviAII | CATG | 6 cut(s) | 372, 496, 827, 910, 1087, 1117 |
| CviQI | GTAC | 3 cut(s) | 246, 820, 1084 |
| DdeI | CTNAG | 6 cut(s) | 111, 177, 256, 451, 779, 784 |
| DpnI | GATC | 2 cut(s) | 282, 791 |
| DpnII | GATC | 2 cut(s) | 280, 789 |
| EaeI | YGGCCR | 2 cut(s) | 3, 612 |
| Eco32I | GATATC | 1 cut(s) | 692 |
| Eco57I | CTGAAG | 1 cut(s) | 462 |
| Eco81I | CCTNAGG | 1 cut(s) | 111 |
| EcoRII | CCWGG | 1 cut(s) | 79 |
| EcoRV | GATATC | 1 cut(s) | 692 |
| FaeI | CATG | 6 cut(s) | 375, 499, 830, 913, 1090, 1120 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 995, 1027 |
| FaqI | GGGAC | 2 cut(s) | 248, 311 |
| FatI | CATG | 6 cut(s) | 371, 495, 826, 909, 1086, 1116 |
| Fnu4HI | GCNGC | 4 cut(s) | 503, 594, 686, 1131 |
| FokI | GGATG | 5 cut(s) | 3, 108, 194, 238, 256 |
| Fsp4HI | GCNGC | 4 cut(s) | 503, 594, 686, 1131 |
| FspBI | CTAG | 3 cut(s) | 159, 560, 741 |
| GlaI | GCGC | 2 cut(s) | 370, 406 |
| GluI | GCNGC | 4 cut(s) | 503, 594, 686, 1131 |
| HaeII | RGCGCY | 1 cut(s) | 408 |
| HaeIII | GGCC | 3 cut(s) | 5, 614, 884 |
| HapII | CCGG | 1 cut(s) | 87 |
| HhaI | GCGC | 2 cut(s) | 371, 407 |
| Hin1I | GRCGYC | 1 cut(s) | 699 |
| Hin1II | CATG | 6 cut(s) | 375, 499, 830, 913, 1090, 1120 |
| Hin6I | GCGC | 2 cut(s) | 369, 405 |
| HinP1I | GCGC | 2 cut(s) | 369, 405 |
| HincII | GTYRAC | 1 cut(s) | 352 |
| HindII | GTYRAC | 1 cut(s) | 352 |
| HinfI | GANTC | 4 cut(s) | 446, 488, 835, 958 |
| HpaII | CCGG | 1 cut(s) | 87 |
| HphI | GGTGA | 3 cut(s) | 378, 542, 953 |
| Hpy166II | GTNNAC | 3 cut(s) | 352, 463, 602 |
| Hpy188I | TCNGA | 4 cut(s) | 259, 313, 345, 454 |
| Hpy188III | TCNNGA | 9 cut(s) | 179, 485, 496, 628, 695, 725, 800, 995, 1112 |
| Hpy8I | GTNNAC | 3 cut(s) | 352, 463, 602 |
| HpyAV | CCTTC | 6 cut(s) | 33, 56, 135, 276, 393, 751 |
| HpyCH4III | ACNGT | 2 cut(s) | 349, 680 |
| HpyCH4IV | ACGT | 3 cut(s) | 61, 540, 699 |
| HpyCH4V | TGCA | 7 cut(s) | 293, 463, 593, 826, 866, 1064, 1130 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 499, 997 |
| HpyF3I | CTNAG | 6 cut(s) | 111, 177, 256, 451, 779, 784 |
| HpySE526I | ACGT | 3 cut(s) | 61, 540, 699 |
| Hsp92I | GRCGYC | 1 cut(s) | 699 |
| Hsp92II | CATG | 6 cut(s) | 375, 499, 830, 913, 1090, 1120 |
| HspAI | GCGC | 2 cut(s) | 369, 405 |
| Kzo9I | GATC | 2 cut(s) | 280, 789 |
| Lsp1109I | GCAGC | 4 cut(s) | 514, 605, 672, 1142 |
| MaeI | CTAG | 3 cut(s) | 159, 560, 741 |
| MaeII | ACGT | 3 cut(s) | 61, 540, 699 |
| MaeIII | GTNAC | 4 cut(s) | 115, 172, 548, 670 |
| MalI | GATC | 2 cut(s) | 282, 791 |
| MbiI | CCGCTC | 1 cut(s) | 1000 |
| MboI | GATC | 2 cut(s) | 280, 789 |
| MboII | GAAGA | 3 cut(s) | 191, 455, 1015 |
| MhlI | GDGCHC | 1 cut(s) | 465 |
| MlsI | TGGCCA | 1 cut(s) | 614 |
| MluCI | AATT | 4 cut(s) | 476, 524, 807, 900 |
| MluNI | TGGCCA | 1 cut(s) | 614 |
| MlyI | GAGTC | 1 cut(s) | 967 |
| MmeI | TCCRAC | 1 cut(s) | 915 |
| Mox20I | TGGCCA | 1 cut(s) | 614 |
| MroXI | GAANNNNTTC | 1 cut(s) | 856 |
| MscI | TGGCCA | 1 cut(s) | 614 |
| MseI | TTAA | 3 cut(s) | 105, 528, 971 |
| MslI | CAYNNNNRTG | 4 cut(s) | 396, 851, 871, 1121 |
| Msp20I | TGGCCA | 1 cut(s) | 614 |
| MspI | CCGG | 1 cut(s) | 87 |
| MspR9I | CCNGG | 1 cut(s) | 81 |
| Mva1269I | GAATGC | 1 cut(s) | 919 |
| MvaI | CCWGG | 1 cut(s) | 81 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 499, 997 |
| NdeII | GATC | 2 cut(s) | 280, 789 |
| NlaIII | CATG | 6 cut(s) | 375, 499, 830, 913, 1090, 1120 |
| NlaIV | GGNNCC | 1 cut(s) | 85 |
| NmeAIII | GCCGAG | 1 cut(s) | 819 |
| NmuCI | GTSAC | 3 cut(s) | 115, 172, 548 |
| NspI | RCATGY | 2 cut(s) | 830, 1120 |
| NspV | TTCGAA | 1 cut(s) | 653 |
| PagI | TCATGA | 1 cut(s) | 495 |
| PciI | ACATGT | 1 cut(s) | 1116 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 67 |
| PctI | GAATGC | 1 cut(s) | 919 |
| PdmI | GAANNNNTTC | 1 cut(s) | 856 |
| PfeI | GAWTC | 3 cut(s) | 446, 488, 835 |
| PflFI | GACNNNGTC | 1 cut(s) | 60 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 372 |
| PkrI | GCNGC | 4 cut(s) | 504, 595, 687, 1132 |
| PleI | GAGTC | 1 cut(s) | 966 |
| PpsI | GAGTC | 1 cut(s) | 966 |
| Ppu21I | YACGTR | 1 cut(s) | 541 |
| PscI | ACATGT | 1 cut(s) | 1116 |
| Psp6I | CCWGG | 1 cut(s) | 79 |
| PspGI | CCWGG | 1 cut(s) | 79 |
| PspN4I | GGNNCC | 1 cut(s) | 85 |
| PstI | CTGCAG | 2 cut(s) | 295, 1132 |
| PsyI | GACNNNGTC | 1 cut(s) | 60 |
| RsaI | GTAC | 3 cut(s) | 247, 821, 1085 |
| RsaNI | GTAC | 3 cut(s) | 246, 820, 1084 |
| RseI | CAYNNNNRTG | 4 cut(s) | 396, 851, 871, 1121 |
| SaqAI | TTAA | 3 cut(s) | 105, 528, 971 |
| SatI | GCNGC | 4 cut(s) | 503, 594, 686, 1131 |
| Sau3AI | GATC | 2 cut(s) | 280, 789 |
| SbfI | CCTGCAGG | 1 cut(s) | 295 |
| SchI | GAGTC | 1 cut(s) | 967 |
| ScrFI | CCNGG | 1 cut(s) | 81 |
| SdaI | CCTGCAGG | 1 cut(s) | 295 |
| SduI | GDGCHC | 1 cut(s) | 465 |
| SfcI | CTRYAG | 2 cut(s) | 291, 1128 |
| SfuI | TTCGAA | 1 cut(s) | 653 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 396, 851, 871, 1121 |
| SmlI | CTYRAG | 5 cut(s) | 554, 769, 800, 955, 1110 |
| SmoI | CTYRAG | 5 cut(s) | 554, 769, 800, 955, 1110 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 295 |
| Sse9I | AATT | 4 cut(s) | 476, 524, 807, 900 |
| SsiI | CCGC | 2 cut(s) | 876, 1000 |
| SspMI | CTAG | 3 cut(s) | 159, 560, 741 |
| StyD4I | CCNGG | 1 cut(s) | 79 |
| TaaI | ACNGT | 2 cut(s) | 349, 680 |
| TaiI | ACGT | 3 cut(s) | 64, 543, 702 |
| TaqI | TCGA | 3 cut(s) | 396, 416, 653 |
| TasI | AATT | 4 cut(s) | 476, 524, 807, 900 |
| TatI | WGTACW | 1 cut(s) | 1083 |
| TfiI | GAWTC | 3 cut(s) | 446, 488, 835 |
| Tru1I | TTAA | 3 cut(s) | 105, 528, 971 |
| Tru9I | TTAA | 3 cut(s) | 105, 528, 971 |
| TseFI | GTSAC | 3 cut(s) | 115, 172, 548 |
| TseI | GCWGC | 4 cut(s) | 502, 593, 685, 1130 |
| Tsp45I | GTSAC | 3 cut(s) | 115, 172, 548 |
| TspDTI | ATGAA | 6 cut(s) | 185, 198, 258, 512, 926, 998 |
| TspGWI | ACGGA | 1 cut(s) | 1111 |
| Tth111I | GACNNNGTC | 1 cut(s) | 60 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 372 |
| VneI | GTGCAC | 1 cut(s) | 461 |
| XapI | RAATTY | 2 cut(s) | 524, 900 |
| XceI | RCATGY | 2 cut(s) | 830, 1120 |
| XmnI | GAANNNNTTC | 1 cut(s) | 856 |
| XspI | CTAG | 3 cut(s) | 159, 560, 741 |
| ZraI | GACGTC | 1 cut(s) | 700 |