Rh6DG166300

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000328
Physical Location & Seq
Forward (+)
25600750 .. 25631168
30419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG166300.1

Sequence Viewer

Length: 720 bp
ATGGAATTCATCCTTAGTCATGAATATCAGCTTTACTTCACTACATGGAATAGACCATTCTGCACGAATAATCAACTTGATAATAATCATCCACTAGAAGAGCGCAAAAAGGTTCTGGACATTAGTGAGGTTCAGACCTATGTGATAAACAGTGTTAGAGTTCTCTTCTTGAATGAGAGGCCTCAACCAAAAGCTGGAATCAAAGGAGTCCTCCACATTTGCTTAATCCTACTTCGGCTATTATGTACAGCACCAAAGAGAATAGAAGACAAAGGGAGGGATTATGGAAAATACATCAGATCAGGGGAGCATGTATGGTTTGTGAGAGAGACTGTACCACAAATTGCAAAGCCTGAAATCATTCGCCGGATAGCAAATTTTCAACCAAGCATTTGGGGAGATCAGTTCATCAACTATGATTCCCAAAAAACTAAGACTGGTGCCCATTGTCAGCAGCAAGTTGACAAATTGAAAACAGTAGTGAAGAGGGAACTCTTTACTAATGAAGGTGATTTTTCACATCGACTCAAGTTAATTGATGCAATCCAACGACTTGGCGTGGCATATCATTTTGAACGCGAAATAGAAGAATCATTGCAACATATCCATGCGACATATCGTGATCGGGATCTGTACCGTGATGATGGCCATCTTTATGATGTTGCCCTATTGTTTCGGCTTCTAAGGCAACATGGATATCAAATTTCATCTGGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

239

Amino Acids

28.25

Weight (kDa)

8.8

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLATZ PF04640 32 - 81 5.3e-10 PLATZ transcription factor
Terpene_synth PF01397 131 - 237 3.7e-28 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 440
AccB7I CCANNNNNTGG 1 cut(s) 194
AccII CGCG 1 cut(s) 579
AclWI GGATC 1 cut(s) 636
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 3 cut(s) 5, 376, 702
AfaI GTAC 4 cut(s) 247, 336, 635, 715
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 4 cut(s) 172, 383, 472, 575
AluBI AGCT 2 cut(s) 31, 194
AluI AGCT 2 cut(s) 31, 194
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 1 cut(s) 636
AoxI GGCC 2 cut(s) 179, 646
ApeKI GCWGC 1 cut(s) 454
ApoI RAATTY 3 cut(s) 5, 376, 702
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 521
BaeGI GKGCMC 1 cut(s) 445
BalI TGGCCA 1 cut(s) 648
BanI GGYRCC 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 466
BccI CCATC 2 cut(s) 638, 657
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 1 cut(s) 95
BisI GCNGC 1 cut(s) 455
BlsI GCNGC 1 cut(s) 456
BmiI GGNNCC 1 cut(s) 442
BmsI GCATC 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 512
BsaBI GATNNNNATC 3 cut(s) 84, 627, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 593
Bse8I GATNNNNATC 3 cut(s) 84, 627, 648
BseGI GGATG 2 cut(s) 9, 88
BseJI GATNNNNATC 3 cut(s) 84, 627, 648
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 593
BseNI ACTGG 1 cut(s) 442
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 2 cut(s) 181, 648
BshNI GGYRCC 1 cut(s) 440
BsiSI CCGG 1 cut(s) 367
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 323
BsnI GGCC 2 cut(s) 181, 648
Bsp1286I GDGCHC 1 cut(s) 445
Bsp1407I TGTACA 1 cut(s) 245
Bsp143I GATC 4 cut(s) 299, 400, 622, 628
BspANI GGCC 2 cut(s) 181, 648
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 442
BspPI GGATC 1 cut(s) 636
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 593
BsrGI TGTACA 1 cut(s) 245
BsrI ACTGG 1 cut(s) 442
BssMI GATC 4 cut(s) 299, 400, 622, 628
Bst4CI ACNGT 4 cut(s) 152, 334, 478, 638
Bst6I CTCTTC 3 cut(s) 93, 170, 479
BstAUI TGTACA 1 cut(s) 245
BstDEI CTNAG 3 cut(s) 14, 432, 683
BstF5I GGATG 2 cut(s) 9, 88
BstFNI CGCG 1 cut(s) 579
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 4 cut(s) 302, 403, 625, 631
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 4 cut(s) 299, 400, 622, 628
BstMWI GCNNNNNNNGC 1 cut(s) 685
BstNSI RCATGY 1 cut(s) 314
BstSLI GKGCMC 1 cut(s) 445
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 628
BstXI CCANNNNNNTGG 2 cut(s) 393, 554
BstYI RGATCY 1 cut(s) 628
BsuRI GGCC 2 cut(s) 181, 648
BtsCI GGATG 2 cut(s) 9, 88
BtsIMutI CAGTG 1 cut(s) 157
CciI TCATGA 1 cut(s) 19
CfoI GCGC 1 cut(s) 105
Csp6I GTAC 4 cut(s) 246, 335, 634, 714
CviAII CATG 5 cut(s) 20, 45, 311, 608, 692
CviJI RGCY 7 cut(s) 31, 181, 194, 238, 352, 648, 679
CviKI_1 RGCY 7 cut(s) 31, 181, 194, 238, 352, 648, 679
CviQI GTAC 4 cut(s) 246, 335, 634, 714
DdeI CTNAG 3 cut(s) 14, 432, 683
DpnI GATC 4 cut(s) 301, 402, 624, 630
DpnII GATC 4 cut(s) 299, 400, 622, 628
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 3 cut(s) 93, 170, 479
EarI CTCTTC 3 cut(s) 93, 170, 479
Eco147I AGGCCT 1 cut(s) 181
Eco32I GATATC 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 5
EcoRV GATATC 1 cut(s) 698
FaeI CATG 5 cut(s) 23, 48, 314, 611, 695
FatI CATG 5 cut(s) 19, 44, 310, 607, 691
Fnu4HI GCNGC 1 cut(s) 455
FokI GGATG 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 455
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 104
GluI GCNGC 1 cut(s) 455
HaeIII GGCC 2 cut(s) 181, 648
HapII CCGG 1 cut(s) 367
HhaI GCGC 1 cut(s) 105
Hin1II CATG 5 cut(s) 23, 48, 314, 611, 695
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 5 cut(s) 198, 207, 419, 525, 590
HpaII CCGG 1 cut(s) 367
HphI GGTGA 1 cut(s) 521
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 135, 299
Hpy188III TCNNGA 5 cut(s) 20, 116, 169, 620, 626
Hpy8I GTNNAC 1 cut(s) 463
HpyAV CCTTC 1 cut(s) 500
HpyCH4III ACNGT 4 cut(s) 152, 334, 478, 638
HpyCH4V TGCA 4 cut(s) 63, 347, 542, 598
HpyF10VI GCNNNNNNNGC 1 cut(s) 685
HpyF3I CTNAG 3 cut(s) 14, 432, 683
Hsp92II CATG 5 cut(s) 23, 48, 314, 611, 695
HspAI GCGC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 299, 400, 622, 628
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 307
LpnPI CCDG 7 cut(s) 101, 180, 288, 366, 380, 423, 696
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 1 cut(s) 529
MaeI CTAG 1 cut(s) 95
MalI GATC 4 cut(s) 301, 402, 624, 630
MboI GATC 4 cut(s) 299, 400, 622, 628
MboII GAAGA 5 cut(s) 110, 157, 278, 496, 599
MflI RGATCY 1 cut(s) 628
MhlI GDGCHC 1 cut(s) 445
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 6 cut(s) 5, 342, 376, 467, 534, 702
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 216, 519
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 6 cut(s) 121, 171, 192, 221, 270, 480
Mox20I TGGCCA 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 648
MseI TTAA 3 cut(s) 224, 533, 718
MslI CAYNNNNRTG 2 cut(s) 606, 654
Msp20I TGGCCA 1 cut(s) 648
MspI CCGG 1 cut(s) 367
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 1 cut(s) 685
NdeII GATC 4 cut(s) 299, 400, 622, 628
NlaIII CATG 5 cut(s) 23, 48, 314, 611, 695
NlaIV GGNNCC 1 cut(s) 442
NspI RCATGY 1 cut(s) 314
PagI TCATGA 1 cut(s) 19
PceI AGGCCT 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 198, 419, 590
PflMI CCANNNNNTGG 1 cut(s) 194
PkrI GCNGC 1 cut(s) 456
PleI GAGTC 2 cut(s) 215, 519
PpsI GAGTC 2 cut(s) 215, 519
PspN4I GGNNCC 1 cut(s) 442
PsuI RGATCY 1 cut(s) 628
RsaI GTAC 4 cut(s) 247, 336, 635, 715
RsaNI GTAC 4 cut(s) 246, 335, 634, 714
RseI CAYNNNNRTG 2 cut(s) 606, 654
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 3 cut(s) 224, 533, 718
SatI GCNGC 1 cut(s) 455
Sau3AI GATC 4 cut(s) 299, 400, 622, 628
SchI GAGTC 2 cut(s) 216, 519
SduI GDGCHC 1 cut(s) 445
SetI ASST 6 cut(s) 33, 114, 132, 140, 196, 511
SfaNI GCATC 1 cut(s) 529
SmiMI CAYNNNNRTG 2 cut(s) 606, 654
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 6 cut(s) 5, 342, 376, 467, 534, 702
SseBI AGGCCT 1 cut(s) 181
SspMI CTAG 1 cut(s) 95
StuI AGGCCT 1 cut(s) 181
TaaI ACNGT 4 cut(s) 152, 334, 478, 638
TaqI TCGA 1 cut(s) 523
TasI AATT 6 cut(s) 5, 342, 376, 467, 534, 702
TatI WGTACW 1 cut(s) 245
TfiI GAWTC 3 cut(s) 198, 419, 590
Tru1I TTAA 3 cut(s) 224, 533, 718
Tru9I TTAA 3 cut(s) 224, 533, 718
TscAI CASTG 1 cut(s) 157
TseI GCWGC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 36, 397, 519, 696
TspRI CASTG 1 cut(s) 157
Van91I CCANNNNNTGG 1 cut(s) 194
XapI RAATTY 3 cut(s) 5, 376, 702
XceI RCATGY 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.