Regulator of Vps4 activity in the MVB pathway
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 2 cut(s) | 224, 233 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 104 |
| AoxI | GGCC | 1 cut(s) | 244 |
| ApeKI | GCWGC | 1 cut(s) | 113 |
| AseI | ATTAAT | 1 cut(s) | 255 |
| AsuHPI | GGTGA | 2 cut(s) | 127, 175 |
| BbvI | GCAGC | 1 cut(s) | 100 |
| BccI | CCATC | 1 cut(s) | 17 |
| BfaI | CTAG | 1 cut(s) | 170 |
| BisI | GCNGC | 1 cut(s) | 114 |
| BlsI | GCNGC | 1 cut(s) | 115 |
| BmiI | GGNNCC | 1 cut(s) | 7 |
| BpuEI | CTTGAG | 1 cut(s) | 221 |
| Bse1I | ACTGG | 1 cut(s) | 109 |
| BseNI | ACTGG | 1 cut(s) | 109 |
| BseXI | GCAGC | 1 cut(s) | 100 |
| BsgI | GTGCAG | 1 cut(s) | 44 |
| BshFI | GGCC | 1 cut(s) | 246 |
| BsnI | GGCC | 1 cut(s) | 246 |
| BspANI | GGCC | 1 cut(s) | 246 |
| BspLI | GGNNCC | 1 cut(s) | 7 |
| BsrI | ACTGG | 1 cut(s) | 109 |
| Bst6I | CTCTTC | 1 cut(s) | 182 |
| BstV1I | GCAGC | 1 cut(s) | 100 |
| BsuRI | GGCC | 1 cut(s) | 246 |
| CaiI | CAGNNNCTG | 1 cut(s) | 104 |
| CviAII | CATG | 2 cut(s) | 38, 280 |
| CviJI | RGCY | 5 cut(s) | 46, 113, 169, 246, 284 |
| CviKI_1 | RGCY | 5 cut(s) | 46, 113, 169, 246, 284 |
| Eam1104I | CTCTTC | 1 cut(s) | 182 |
| EarI | CTCTTC | 1 cut(s) | 182 |
| Eco57I | CTGAAG | 2 cut(s) | 224, 233 |
| FaeI | CATG | 2 cut(s) | 41, 283 |
| FaiI | YATR | 6 cut(s) | 39, 149, 158, 262, 264, 281 |
| FatI | CATG | 2 cut(s) | 37, 279 |
| Fnu4HI | GCNGC | 1 cut(s) | 114 |
| Fsp4HI | GCNGC | 1 cut(s) | 114 |
| FspBI | CTAG | 1 cut(s) | 170 |
| GluI | GCNGC | 1 cut(s) | 114 |
| HaeIII | GGCC | 1 cut(s) | 246 |
| Hin1II | CATG | 2 cut(s) | 41, 283 |
| HphI | GGTGA | 2 cut(s) | 127, 175 |
| Hpy188I | TCNGA | 2 cut(s) | 145, 213 |
| Hpy188III | TCNNGA | 2 cut(s) | 99, 238 |
| HpyCH4V | TGCA | 1 cut(s) | 25 |
| Hsp92II | CATG | 2 cut(s) | 41, 283 |
| LpnPI | CCDG | 2 cut(s) | 84, 90 |
| Lsp1109I | GCAGC | 1 cut(s) | 100 |
| MaeI | CTAG | 1 cut(s) | 170 |
| MboII | GAAGA | 4 cut(s) | 196, 199, 224, 226 |
| MnlI | CCTC | 4 cut(s) | 36, 159, 183, 260 |
| MseI | TTAA | 3 cut(s) | 180, 255, 272 |
| MslI | CAYNNNNRTG | 1 cut(s) | 161 |
| NlaIII | CATG | 2 cut(s) | 41, 283 |
| NlaIV | GGNNCC | 1 cut(s) | 7 |
| PkrI | GCNGC | 1 cut(s) | 115 |
| PshBI | ATTAAT | 1 cut(s) | 255 |
| PspN4I | GGNNCC | 1 cut(s) | 7 |
| PstNI | CAGNNNCTG | 1 cut(s) | 104 |
| RseI | CAYNNNNRTG | 1 cut(s) | 161 |
| SaqAI | TTAA | 3 cut(s) | 180, 255, 272 |
| SatI | GCNGC | 1 cut(s) | 114 |
| SetI | ASST | 1 cut(s) | 165 |
| SgeI | CNNG | 8 cut(s) | 50, 59, 71, 111, 117, 182, 250, 260 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 161 |
| SmlI | CTYRAG | 1 cut(s) | 236 |
| SmoI | CTYRAG | 1 cut(s) | 236 |
| SspI | AATATT | 1 cut(s) | 128 |
| SspMI | CTAG | 1 cut(s) | 170 |
| Tru1I | TTAA | 3 cut(s) | 180, 255, 272 |
| Tru9I | TTAA | 3 cut(s) | 180, 255, 272 |
| TseI | GCWGC | 1 cut(s) | 113 |
| TspDTI | ATGAA | 3 cut(s) | 26, 110, 268 |
| VspI | ATTAAT | 1 cut(s) | 255 |
| XspI | CTAG | 1 cut(s) | 170 |