FvH4_2g13832

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000328
Physical Location & Seq
Forward (+)
12053645 .. 12055255
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13832.t1

Sequence Viewer

Length: 468 bp
ATGGAGGAGGTGAGTGAGGCTGAGAAGGCCGAACCTTGGTTCGACTCCGGTCTCACCTACGCCTCAACTTCTTCAGTGCAGCGGGGAACTCTGGTTTCCCTAGTACTGCATGCAATGTTGCTCCCTGGATCCTCACTGTTGCTGCAAGCACTTTGGATAGAGAATTCCATTCAAACTTTAGTAGTTTTGGTGAAGTCTCTATTGGCCATAAGAATCAGTGAAGTACTGGATTGGGAGAGCAAAGAGAGAATCTATCCAGCTGCGGTGTTGTTCAAAAGGGAGCAGCTGCAACTCGGTCATGATCGGATCATAAGCAAAACTGATATAAAGACCTTGAAAGTGAGGCTTGAGGGGATTAAGAAGAAGAGGAACTCTGTGCTCAAGTATTTGAAGAATGTTGTTGTTGATCTTCTCAAGAATGGCCTTGACATTAATGCATATGGAAGGGTAAGTTTTATTGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

156

Amino Acids

17.49

Weight (kDa)

9.33

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 82, 263
AclWI GGATC 3 cut(s) 123, 136, 314
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 2 cut(s) 105, 225
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 4 cut(s) 173, 274, 337, 391
AjnI CCWGG 1 cut(s) 124
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 2 cut(s) 260, 286
AluI AGCT 2 cut(s) 260, 286
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 56, 201
AlwI GGATC 3 cut(s) 123, 136, 314
AoxI GGCC 3 cut(s) 27, 204, 421
ApeKI GCWGC 5 cut(s) 79, 142, 260, 283, 286
ApoI RAATTY 1 cut(s) 163
AseI ATTAAT 1 cut(s) 432
AsuHPI GGTGA 3 cut(s) 22, 46, 202
BalI TGGCCA 1 cut(s) 206
BamHI GGATCC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 5 cut(s) 91, 129, 247, 273, 295
BciT130I CCWGG 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 56, 201
BfaI CTAG 1 cut(s) 101
BisI GCNGC 5 cut(s) 80, 143, 261, 284, 287
BlsI GCNGC 5 cut(s) 81, 144, 262, 285, 288
BmcAI AGTACT 2 cut(s) 105, 225
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 48
BpuEI CTTGAG 3 cut(s) 365, 368, 398
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 35, 124
BsaWI WCCGGW 1 cut(s) 47
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 35, 124
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 12
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 5 cut(s) 91, 129, 247, 273, 295
BsgI GTGCAG 1 cut(s) 98
BshFI GGCC 3 cut(s) 29, 206, 423
BsiHKAI GWGCWC 1 cut(s) 381
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 2 cut(s) 56, 201
BsnI GGCC 3 cut(s) 29, 206, 423
Bso31I GGTCTC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 4 cut(s) 128, 301, 306, 406
BspACI CCGC 2 cut(s) 82, 263
BspANI GGCC 3 cut(s) 29, 206, 423
BspCNI CTCAG 1 cut(s) 13
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 3 cut(s) 123, 136, 314
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 35, 124
BssMI GATC 4 cut(s) 128, 301, 306, 406
BssT1I CCWWGG 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 111, 147
BstDEI CTNAG 1 cut(s) 21
BstKTI GATC 4 cut(s) 131, 304, 309, 409
BstMAI GTCTC 2 cut(s) 56, 201
BstMBI GATC 4 cut(s) 128, 301, 306, 406
BstMWI GCNNNNNNNGC 1 cut(s) 26
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 113
BstPAI GACNNNNGTC 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 124
BstV1I GCAGC 5 cut(s) 91, 129, 247, 273, 295
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
BsuRI GGCC 3 cut(s) 29, 206, 423
BtsIMutI CAGTG 3 cut(s) 81, 134, 223
Cac8I GCNNGC 2 cut(s) 111, 147
CciI TCATGA 1 cut(s) 298
Csp6I GTAC 2 cut(s) 104, 224
CviAII CATG 2 cut(s) 110, 299
CviJI RGCY 7 cut(s) 20, 29, 206, 260, 286, 346, 423
CviKI_1 RGCY 7 cut(s) 20, 29, 206, 260, 286, 346, 423
CviQI GTAC 2 cut(s) 104, 224
DdeI CTNAG 1 cut(s) 21
DpnI GATC 4 cut(s) 130, 303, 308, 408
DpnII GATC 4 cut(s) 128, 301, 306, 406
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 57
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 35
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 35
FaeI CATG 2 cut(s) 113, 302
FaiI YATR 7 cut(s) 111, 209, 300, 311, 326, 439, 441
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FatI CATG 2 cut(s) 109, 298
FauI CCCGC 1 cut(s) 75
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 5 cut(s) 80, 143, 261, 284, 287
Fsp4HI GCNGC 5 cut(s) 80, 143, 261, 284, 287
FspBI CTAG 1 cut(s) 101
GluI GCNGC 5 cut(s) 80, 143, 261, 284, 287
HaeIII GGCC 3 cut(s) 29, 206, 423
HapII CCGG 1 cut(s) 48
Hin1II CATG 2 cut(s) 113, 302
HinfI GANTC 3 cut(s) 44, 213, 249
HpaII CCGG 1 cut(s) 48
HphI GGTGA 3 cut(s) 22, 46, 202
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 2 cut(s) 299, 415
HpyAV CCTTC 2 cut(s) 19, 438
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4V TGCA 6 cut(s) 79, 109, 113, 145, 289, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 26
HpyF3I CTNAG 1 cut(s) 21
Hsp92II CATG 2 cut(s) 113, 302
Kzo9I GATC 4 cut(s) 128, 301, 306, 406
LmnI GCTCC 2 cut(s) 126, 280
LpnPI CCDG 6 cut(s) 61, 77, 111, 138, 212, 270
Lsp1109I GCAGC 5 cut(s) 91, 129, 247, 273, 295
MaeI CTAG 1 cut(s) 101
MalI GATC 4 cut(s) 130, 303, 308, 408
MboI GATC 4 cut(s) 128, 301, 306, 406
MboII GAAGA 5 cut(s) 63, 373, 376, 401, 403
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 381
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 1 cut(s) 163
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 1 cut(s) 38
MnlI CCTC 6 cut(s) 10, 73, 142, 336, 343, 360
Mox20I TGGCCA 1 cut(s) 206
Mph1103I ATGCAT 1 cut(s) 439
MscI TGGCCA 1 cut(s) 206
MseI TTAA 2 cut(s) 357, 432
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 3 cut(s) 82, 260, 286
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 126
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 26
NdeI CATATG 1 cut(s) 439
NdeII GATC 4 cut(s) 128, 301, 306, 406
NlaIII CATG 2 cut(s) 113, 302
NlaIV GGNNCC 1 cut(s) 130
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 113
PaeI GCATGC 1 cut(s) 113
PagI TCATGA 1 cut(s) 298
PfeI GAWTC 2 cut(s) 213, 249
PkrI GCNGC 5 cut(s) 81, 144, 262, 285, 288
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PshAI GACNNNNGTC 1 cut(s) 48
PshBI ATTAAT 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 130
PsuI RGATCY 1 cut(s) 128
PvuII CAGCTG 2 cut(s) 260, 286
RsaI GTAC 2 cut(s) 105, 225
RsaNI GTAC 2 cut(s) 104, 224
SaqAI TTAA 2 cut(s) 357, 432
SatI GCNGC 5 cut(s) 80, 143, 261, 284, 287
Sau3AI GATC 4 cut(s) 128, 301, 306, 406
ScaI AGTACT 2 cut(s) 105, 225
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 381
SetI ASST 6 cut(s) 12, 37, 59, 262, 288, 335
SmlI CTYRAG 3 cut(s) 347, 380, 413
SmoI CTYRAG 3 cut(s) 347, 380, 413
SphI GCATGC 1 cut(s) 113
Sse9I AATT 1 cut(s) 163
SsiI CCGC 2 cut(s) 82, 263
SspMI CTAG 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 42
TaqII GACCGA 1 cut(s) 284
TasI AATT 1 cut(s) 163
TatI WGTACW 2 cut(s) 103, 223
TfiI GAWTC 2 cut(s) 213, 249
Tru1I TTAA 2 cut(s) 357, 432
Tru9I TTAA 2 cut(s) 357, 432
TscAI CASTG 3 cut(s) 81, 141, 223
TseI GCWGC 5 cut(s) 79, 142, 260, 283, 286
TspDTI ATGAA 1 cut(s) 452
TspRI CASTG 3 cut(s) 81, 141, 223
VspI ATTAAT 1 cut(s) 432
XapI RAATTY 1 cut(s) 163
XceI RCATGY 1 cut(s) 113
XspI CTAG 1 cut(s) 101
ZrmI AGTACT 2 cut(s) 105, 225
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.