Rorug02G0050600

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000328
Physical Location & Seq
Forward (+)
3892347 .. 3894633
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0050600.1

Sequence Viewer

Length: 651 bp
ATGAAGAAGACCTCGAAAGGCGAGTCTGCATTCTACGCGCCGATTCCGGCCAACCCCGACCACCCCCAACACGTCGTCGTTTTAACTCATTACCGCGGCCCCTCTCCCGACTACCACGAACGACGTCGTCTCCGCCTCTGCGTATCCACCACCGTCGCTTTCGTCCTCCTCTCCGCCGCCGCGTTCTTCCTCTTCCCCTCCGACCCGGCCCTAGAGCTCGCCCGGATCCACCTCAACCACGTCGGAGTCCACTCGTCGCCGAAGCTCACTCTGGACCTATCGTTCTCGCTGACGATCAGGGTCCGGAATCGCGACTTCTTCTCTCTGGACTACGACTCGCTGGTGGTCAAAATCGGGTACCGGGGGAGAGAGCTAGGGTTTGTGAGCTCGGCCGGCGGGCGCGTGAGGGCCAGAGGGTCGTCGTACGTGAACGCCACGCTCGTGCTGGATGGGCTGGGGGTCATTCACGACGTGTTTTACTTGCTTGAAGATTTGGCTAGGGGTGTGATTCCCTTTGATACTGATACGGAGGTGGATGGAACTGTTGGGCTCTTTGTCTTCAAAATTCCTATCAAGGGAAGAGCTTCGTGTGAGGTGTATGTGAATACAAACAATCAGACAGTTGTTCGTCAAGATTGTTACCCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

216

Amino Acids

24.01

Weight (kDa)

8.43

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 98 - 192 2.8e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13832 FvH4_3g26701 FvH4_4g14350 FvH4_7g17370
malus_domestica MD12G1077900.v1.1 MD14G1070200.v1.1
prunus_persica Prupe.2G073100_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1 Prupe.2G186800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365191 RchiOBHm_Chr2g0105141 RchiOBHm_Chr2g0120431 RchiOBHm_Chr2g0139381 RchiOBHm_Chr3g0494421 RchiOBHm_Chr6g0248181 RchiOBHm_Chr7g0235571
rosa_laevigata RLG00000005158 RLG00000008937 RLG00000013742 RLG00000015899 RLG00000019885 RLG00000024515 RLG00000024516 RLG00000028740 RLG00000030656 RLG00000030662 RLG00000032758 RLG00000035668 RLG00000036717 RLG00000036718
rosa_multiflora Rmu_sc0000252.1_g000016 Rmu_sc0001386.1_g000020 Rmu_sc0002271.1_g000010 Rmu_sc0003833.1_g000002 Rmu_sc0004718.1_g000004 Rmu_sc0006031.1_g000006 Rmu_sc0006598.1_g000013 Rmu_sc0007472.1_g000023 Rmu_sc0009313.1_g000012
rosa_roxburghii Rroxscaffold_2G00103830 Rroxscaffold_2G00109880 Rroxscaffold_3G00225910 Rroxscaffold_3G00271600 Rroxscaffold_3G00274560 Rroxscaffold_4G00299580 Rroxscaffold_5G00352530 Rroxscaffold_5G00379280 Rroxscaffold_7G00177320
rosa_rugosa Rorug01G0027200 Rorug01G0032100 Rorug02G0050600 Rorug02G0054800 Rorug04G0083300 Rorug05G0115200 Rorug07G0006800
rosa_samantha Rh1CG086700 Rh1DG093300 Rh1DG217000 Rh2AG120200 Rh2AG227900 Rh2AG254000 Rh2AG434200 Rh2AG469200 Rh2BG159500 Rh2CG419900 Rh2CG455200 Rh2DG320100 Rh2DG603500 Rh3CG319900 Rh3DG300800 Rh3DG319200 Rh4AG199400 Rh4BG329100 Rh4BG329200 Rh4CG110800 Rh4DG042000 Rh4DG129400 Rh4DG187700 Rh4DG231900 Rh5AG038800 Rh5AG107300 Rh5AG303600 Rh5AG386500 Rh5AG454200 Rh5CG041800 Rh5CG534800 Rh5DG076400 Rh5DG109800 Rh5DG123600 Rh6AG164400 Rh6BG035100 Rh6BG036000 Rh6BG041000 Rh6BG215500 Rh6CG173900 Rh6CG174000 Rh6DG020300 Rh6DG166300 Rh6DG320000 Rh6DG509700 Rh7AG059500 Rh7AG291700 Rh7BG404300 Rh7DG402000
rosa_wichuraiana Rw1G000890 Rw1G020020 Rw3G005730 Rw4G011330 Rw4G026960 Rw5G014010 Rw6G002230 Rw7G038480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 127
Acc65I GGTACC 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 357
AccII CGCG 5 cut(s) 38, 96, 182, 312, 402
AccIII TCCGGA 1 cut(s) 303
AciI CCGC 7 cut(s) 94, 96, 133, 174, 177, 180, 396
AclWI GGATC 2 cut(s) 220, 233
AcoI YGGCCR 2 cut(s) 48, 390
AcsI RAATTY 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 124
AdeI CACNNNGTG 1 cut(s) 472
AfaI GTAC 2 cut(s) 359, 425
AfiI CCNNNNNNNGG 1 cut(s) 575
AflIII ACRYGT 2 cut(s) 70, 471
AgsI TTSAA 2 cut(s) 488, 562
AjiI CACGTC 3 cut(s) 73, 241, 472
AleI CACNNNNGTG 1 cut(s) 440
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 5 cut(s) 217, 265, 373, 387, 584
AluI AGCT 5 cut(s) 217, 265, 373, 387, 584
Alw21I GWGCWC 2 cut(s) 219, 389
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 2 cut(s) 220, 233
Aor13HI TCCGGA 1 cut(s) 303
AoxI GGCC 5 cut(s) 48, 97, 207, 390, 408
ApoI RAATTY 1 cut(s) 564
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
Asp700I GAANNNNTTC 1 cut(s) 583
Asp718I GGTACC 1 cut(s) 357
AspLEI GCGC 2 cut(s) 40, 402
AspS9I GGNCC 5 cut(s) 98, 208, 274, 301, 408
AsuC2I CCSGG 3 cut(s) 206, 223, 362
AvaII GGWCC 2 cut(s) 274, 301
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BamHI GGATCC 1 cut(s) 225
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 3 cut(s) 219, 389, 552
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 2 cut(s) 14, 550
Bbv12I GWGCWC 2 cut(s) 219, 389
BccI CCATC 2 cut(s) 443, 530
BciVI GTATCC 1 cut(s) 154
BcnI CCSGG 3 cut(s) 206, 223, 362
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 3 cut(s) 212, 374, 498
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 3 cut(s) 97, 177, 180
BlsI GCNGC 3 cut(s) 98, 178, 181
Bme1390I CCNGG 3 cut(s) 206, 223, 362
Bme18I GGWCC 2 cut(s) 274, 301
BmgBI CACGTC 3 cut(s) 73, 241, 472
BmgT120I GGNCC 5 cut(s) 98, 208, 274, 301, 408
BmiI GGNNCC 4 cut(s) 100, 227, 302, 359
BmrFI CCNGG 3 cut(s) 206, 223, 362
BpiI GAAGAC 2 cut(s) 14, 550
BpuMI CCSGG 3 cut(s) 206, 223, 362
BsaAI YACGTR 1 cut(s) 427
BsaHI GRCGYC 1 cut(s) 124
BsaJI CCNNGG 2 cut(s) 94, 361
BsaWI WCCGGW 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 392
BseAI TCCGGA 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 94, 361
BseGI GGATG 2 cut(s) 454, 541
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMII CTCAG 1 cut(s) 636
BseRI GAGGAG 1 cut(s) 158
BseX3I CGGCCG 1 cut(s) 390
BseYI CCCAGC 1 cut(s) 454
Bsh1236I CGCG 5 cut(s) 38, 96, 182, 312, 402
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 5 cut(s) 50, 99, 209, 392, 410
BshNI GGYRCC 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 219, 389
BsiSI CCGG 6 cut(s) 47, 206, 223, 304, 361, 393
BsiWI CGTACG 1 cut(s) 423
BslI CCNNNNNNNGG 1 cut(s) 575
BsmAI GTCTC 1 cut(s) 134
BsmBI CGTCTC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 5 cut(s) 50, 99, 209, 392, 410
Bsp1286I GDGCHC 3 cut(s) 219, 389, 552
Bsp13I TCCGGA 1 cut(s) 303
Bsp143I GATC 2 cut(s) 225, 294
Bsp68I TCGCGA 1 cut(s) 312
BspACI CCGC 7 cut(s) 94, 96, 133, 174, 177, 180, 396
BspANI GGCC 5 cut(s) 50, 99, 209, 392, 410
BspCNI CTCAG 1 cut(s) 637
BspEI TCCGGA 1 cut(s) 303
BspFNI CGCG 5 cut(s) 38, 96, 182, 312, 402
BspLI GGNNCC 4 cut(s) 100, 227, 302, 359
BspPI GGATC 2 cut(s) 220, 233
BspQI GCTCTTC 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 392
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 94, 361
BssMI GATC 2 cut(s) 225, 294
BssNI GRCGYC 1 cut(s) 124
BssSI CACGAG 1 cut(s) 440
Bst2BI CACGAG 1 cut(s) 440
Bst4CI ACNGT 3 cut(s) 154, 544, 622
Bst6I CTCTTC 2 cut(s) 197, 574
BstACI GRCGYC 1 cut(s) 124
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 219, 394, 398
BstDEI CTNAG 1 cut(s) 645
BstDSI CCRYGG 1 cut(s) 94
BstF5I GGATG 2 cut(s) 454, 541
BstFNI CGCG 5 cut(s) 38, 96, 182, 312, 402
BstHHI GCGC 2 cut(s) 40, 402
BstKTI GATC 2 cut(s) 228, 297
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 225, 294
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 3 cut(s) 35, 393, 451
BstSCI CCNGG 3 cut(s) 204, 221, 360
BstUI CGCG 5 cut(s) 38, 96, 182, 312, 402
BstV2I GAAGAC 2 cut(s) 14, 550
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BstZI CGGCCG 1 cut(s) 390
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 5 cut(s) 50, 99, 209, 392, 410
BtgI CCRYGG 1 cut(s) 94
BtrI CACGTC 3 cut(s) 73, 241, 472
BtsCI GGATG 2 cut(s) 454, 541
BtuMI TCGCGA 1 cut(s) 312
Cac8I GCNNGC 3 cut(s) 219, 394, 398
CfoI GCGC 2 cut(s) 40, 402
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 5 cut(s) 98, 208, 274, 301, 408
Cfr42I CCGCGG 1 cut(s) 97
Csp6I GTAC 2 cut(s) 358, 424
CviQI GTAC 2 cut(s) 358, 424
DdeI CTNAG 1 cut(s) 645
DpnI GATC 2 cut(s) 227, 296
DpnII GATC 2 cut(s) 225, 294
DraIII CACNNNGTG 1 cut(s) 472
EaeI YGGCCR 2 cut(s) 48, 390
EagI CGGCCG 1 cut(s) 390
Eam1104I CTCTTC 2 cut(s) 197, 574
EarI CTCTTC 2 cut(s) 197, 574
EciI GGCGGA 2 cut(s) 122, 163
Ecl136II GAGCTC 2 cut(s) 217, 387
EclXI CGGCCG 1 cut(s) 390
Eco24I GRGCYC 3 cut(s) 219, 389, 552
Eco47I GGWCC 2 cut(s) 274, 301
Eco52I CGGCCG 1 cut(s) 390
Eco53kI GAGCTC 2 cut(s) 217, 387
EcoICRI GAGCTC 2 cut(s) 217, 387
EcoT38I GRGCYC 3 cut(s) 219, 389, 552
Esp3I CGTCTC 1 cut(s) 134
FaiI YATR 1 cut(s) 600
FauI CCCGC 1 cut(s) 389
Fnu4HI GCNGC 3 cut(s) 97, 177, 180
FokI GGATG 2 cut(s) 461, 548
FriOI GRGCYC 3 cut(s) 219, 389, 552
Fsp4HI GCNGC 3 cut(s) 97, 177, 180
FspBI CTAG 3 cut(s) 212, 374, 498
GlaI GCGC 2 cut(s) 39, 401
GluI GCNGC 3 cut(s) 97, 177, 180
GsaI CCCAGC 1 cut(s) 458
HaeIII GGCC 5 cut(s) 50, 99, 209, 392, 410
HapII CCGG 6 cut(s) 47, 206, 223, 304, 361, 393
HhaI GCGC 2 cut(s) 40, 402
Hin1I GRCGYC 1 cut(s) 124
Hin6I GCGC 2 cut(s) 38, 400
HinP1I GCGC 2 cut(s) 38, 400
HinfI GANTC 6 cut(s) 23, 43, 246, 307, 335, 508
HpaII CCGG 6 cut(s) 47, 206, 223, 304, 361, 393
Hpy166II GTNNAC 2 cut(s) 250, 430
Hpy188I TCNGA 3 cut(s) 202, 245, 618
Hpy188III TCNNGA 7 cut(s) 107, 272, 304, 311, 326, 467, 632
Hpy8I GTNNAC 2 cut(s) 250, 430
Hpy99I CGWCG 9 cut(s) 77, 80, 126, 129, 158, 245, 259, 424, 473
HpyCH4III ACNGT 3 cut(s) 154, 544, 622
HpyCH4IV ACGT 5 cut(s) 72, 124, 240, 426, 471
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 393, 451
HpyF3I CTNAG 1 cut(s) 645
HpySE526I ACGT 5 cut(s) 72, 124, 240, 426, 471
Hsp92I GRCGYC 1 cut(s) 124
HspAI GCGC 2 cut(s) 38, 400
Kpn2I TCCGGA 1 cut(s) 303
KpnI GGTACC 1 cut(s) 361
KroI GCCGGC 1 cut(s) 392
KroNI GCCGGC 1 cut(s) 394
KspI CCGCGG 1 cut(s) 97
Kzo9I GATC 2 cut(s) 225, 294
LguI GCTCTTC 1 cut(s) 574
MaeI CTAG 3 cut(s) 212, 374, 498
MaeII ACGT 5 cut(s) 72, 124, 240, 426, 471
MaeIII GTNAC 1 cut(s) 638
MalI GATC 2 cut(s) 227, 296
MboI GATC 2 cut(s) 225, 294
MboII GAAGA 8 cut(s) 16, 19, 178, 184, 310, 500, 550, 591
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 3 cut(s) 219, 389, 552
MluCI AATT 1 cut(s) 564
MlyI GAGTC 3 cut(s) 32, 255, 329
MmeI TCCRAC 2 cut(s) 223, 225
MroI TCCGGA 1 cut(s) 303
MroNI GCCGGC 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 583
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 96
MspI CCGG 6 cut(s) 47, 206, 223, 304, 361, 393
MspR9I CCNGG 3 cut(s) 206, 223, 362
Mva1269I GAATGC 1 cut(s) 29
MvnI CGCG 5 cut(s) 38, 96, 182, 312, 402
MwoI GCNNNNNNNGC 3 cut(s) 35, 393, 451
NaeI GCCGGC 1 cut(s) 394
NciI CCSGG 3 cut(s) 206, 223, 362
NdeII GATC 2 cut(s) 225, 294
NgoMIV GCCGGC 1 cut(s) 392
NlaIV GGNNCC 4 cut(s) 100, 227, 302, 359
NmeAIII GCCGAG 1 cut(s) 368
NruI TCGCGA 1 cut(s) 312
OliI CACNNNNGTG 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PctI GAATGC 1 cut(s) 29
PdiI GCCGGC 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 583
PfeI GAWTC 3 cut(s) 43, 307, 508
Pfl23II CGTACG 1 cut(s) 423
PflFI GACNNNGTC 1 cut(s) 126
PkrI GCNGC 3 cut(s) 98, 178, 181
PleI GAGTC 3 cut(s) 31, 254, 329
PpsI GAGTC 3 cut(s) 31, 254, 329
Ppu21I YACGTR 1 cut(s) 427
Psp124BI GAGCTC 2 cut(s) 219, 389
PspFI CCCAGC 1 cut(s) 454
PspLI CGTACG 1 cut(s) 423
PspN4I GGNNCC 4 cut(s) 100, 227, 302, 359
PspPI GGNCC 5 cut(s) 98, 208, 274, 301, 408
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 126
RruI TCGCGA 1 cut(s) 312
RsaI GTAC 2 cut(s) 359, 425
RsaNI GTAC 2 cut(s) 358, 424
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 2 cut(s) 219, 389
SacII CCGCGG 1 cut(s) 97
SapI GCTCTTC 1 cut(s) 574
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 3 cut(s) 97, 177, 180
Sau3AI GATC 2 cut(s) 225, 294
Sau96I GGNCC 5 cut(s) 98, 208, 274, 301, 408
SchI GAGTC 3 cut(s) 32, 255, 329
ScrFI CCNGG 3 cut(s) 206, 223, 362
SduI GDGCHC 3 cut(s) 219, 389, 552
Sfr303I CCGCGG 1 cut(s) 97
SgrBI CCGCGG 1 cut(s) 97
SinI GGWCC 2 cut(s) 274, 301
SmiMI CAYNNNNRTG 1 cut(s) 440
Sse9I AATT 1 cut(s) 564
SsiI CCGC 7 cut(s) 94, 96, 133, 174, 177, 180, 396
SspMI CTAG 3 cut(s) 212, 374, 498
SstI GAGCTC 2 cut(s) 219, 389
StyD4I CCNGG 3 cut(s) 204, 221, 360
TaaI ACNGT 3 cut(s) 154, 544, 622
TaiI ACGT 5 cut(s) 75, 127, 243, 429, 474
TaqI TCGA 1 cut(s) 14
TasI AATT 1 cut(s) 564
TauI GCSGC 3 cut(s) 99, 179, 182
TfiI GAWTC 3 cut(s) 43, 307, 508
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 542
Tth111I GACNNNGTC 1 cut(s) 126
VpaK11BI GGWCC 2 cut(s) 274, 301
XapI RAATTY 1 cut(s) 564
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 583
XspI CTAG 3 cut(s) 212, 374, 498
ZraI GACGTC 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.