pycom02g26070

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008044
Physical Location & Seq
Forward (+)
23917294 .. 23922859
5566 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1641 bp
ATGGCGGAGGTCGAAGTTAACGACGAGAAGCAGCAGCTCAAGAGCAACGCCATGGTGGTCCCTAACCCAAAACCCAGCAAGGGTTTTTCTTCAAAAGTGGTGGACTCGATCGAGAAGCTGATTGTGAAGTGGATGTACAACTCTTCTCGGCCTCACCGCTACCTCGCCGGCAACTTTGCTCCGGTGGCTGATGAAACTCCTCCCACCACCAACCTCCCAGTCATCGGCCATCTTCCTGAGTGCTTAAATGGAGAGTTTGTCAGGGTGGGGCCCAACCCAAAGTTTGCACCAGTTGCTGGATATCACTGGTTTGATGGAGATGGTATGGTTCATGGTATGCGCATAAAAGATGGAAAAGCAACTTATGTCTCCCGCTACGTAAGGACTTCACGTCTTAAGCAAGAACAATATTTTGGAGGTTCTAAATTTATGAAGATTGGAGACCTTAAGGGGTTTCTCGGCTTATTCATGATGAACTTGCAAATGCTAAGAGCAAAACTGAAAATATTAGACATGTCTTATGGTTTTGGGACTGGTAACACGGCTCTCATATATCACCATGGGAAGCTTCTAGCTCTTCACGAGGGGGATAAACCTTATGTCCTCAAAGTTTTGGAAGATGGAGATCTGCAAACGGTTGGCTTGCTGGATTATGATAAGAGATTGACACATTCCTTCACTGCTCATCCAAAGGTTGACCCATTCACCGGTGAGATGTTTACATTTGGCTATTCATCTACTCCGCCATATGTCACTTATAGAGTTATTTCGAAGGATGGTTTCATGCATGACCCTGTACCGATAACAATACCAGACCCCGTCATGATGCATGATTTTGCAATTACTGAGAACTATGCAATTTTCATGGATCTCCCCCTGATCTTCAAACCAAAGGAAATGGTGAAGGAAAAAAAGTTGATATTCACATTTGATCAAACAAAAAAAGCTCGCTTTGGTTTACTTCCACGGTATGCAAAGGATGAGTTGCTAATCAGATGGTTCGAGCTTCCAAATTGCTTCATTTTCCATAATGGCTTGGGAGGAGGGGGACGAAGTTGTTTAATCACATGCCGCCTTGAGAATCTTGATTTGGACTTGGGCAATGGGTCTGTCAAAGAAAAGCTTGAAAACTTGACAAATGAGCTGTACGAGATGAGATTCAACTTGAAAACTGGTCTAGCATCCCAAAAGAAACTATCAGAACCGGCTGTAGATTTCCCCAGGGTGAATGAGAGCTACACCGGCAGGAAGCAGCGGTATGTGTACGTAACCGTACTGGACAACATTGGCAAGGTTACGGGAGTAGCCAAGTTTGATCTGCATGCTGAACCACAGGTTGGAAAAACACAGATTGAGGTTGGAGGAAATGTCCAAGGCCTCTATGACCTGGGACCTGGCAGATTTGGTTCTGAGGCTATTTTTGTTCCTCGTATTCCTGGCGTTACTTCTGAAGAAGATGATGGCTACTTAATACTCTTTGTTCACGACGAGAACACTGGAAAATCAGCAATTCATGTAGTTGATGCAAAGACAATGTCACCAGAGCCCGTTGCAGTTGTGGAATTGCCACACAGAGTTCCATACGGTTTGCATGCATTCTTCGTGACAGAGGAGCAATTGCAAGAACAAGCGAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

547

Amino Acids

61.37

Weight (kDa)

7.0

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 59 - 535 2.5e-130 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 341
AccB7I CCANNNNNTGG 2 cut(s) 296, 1337
AciI CCGC 6 cut(s) 5, 157, 373, 743, 1072, 1255
AclWI GGATC 1 cut(s) 876
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 1 cut(s) 425
AcuI CTGAAG 1 cut(s) 1470
AfaI GTAC 5 cut(s) 137, 798, 1148, 1265, 1275
AfiI CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1337
AflII CTTAAG 2 cut(s) 395, 446
AflIII ACRYGT 1 cut(s) 513
AgeI ACCGGT 1 cut(s) 707
AgsI TTSAA 5 cut(s) 93, 886, 1127, 1162, 1168
AhdI GACNNNNNGTC 1 cut(s) 390
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 4 cut(s) 1220, 1386, 1393, 1435
AjuI GAANNNNNNNTTGG 6 cut(s) 396, 428, 1073, 1105, 1320, 1352
Alw26I GTCTC 2 cut(s) 373, 435
AlwI GGATC 1 cut(s) 876
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 4 cut(s) 149, 226, 269, 1375
ApaI GGGCCC 1 cut(s) 273
ApeKI GCWGC 3 cut(s) 31, 34, 1252
ApoI RAATTY 1 cut(s) 425
ArsI GACNNNNNNTTYG 2 cut(s) 1520, 1552
AsiGI ACCGGT 1 cut(s) 707
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 4 cut(s) 58, 269, 270, 1391
AsuHPI GGTGA 7 cut(s) 146, 548, 697, 722, 913, 1237, 1530
AsuII TTCGAA 1 cut(s) 770
AvaII GGWCC 2 cut(s) 58, 1391
BaeGI GKGCMC 1 cut(s) 273
BanII GRGCYC 2 cut(s) 273, 1548
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BauI CACGAG 1 cut(s) 581
BbvI GCAGC 3 cut(s) 43, 46, 1264
BccI CCATC 8 cut(s) 237, 308, 314, 344, 614, 770, 990, 1454
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 4 cut(s) 1222, 1388, 1395, 1437
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 2 cut(s) 373, 435
BfaI CTAG 2 cut(s) 572, 1178
BfmI CTRYAG 1 cut(s) 1209
BfrI CTTAAG 2 cut(s) 395, 446
BglII AGATCT 1 cut(s) 625
BisI GCNGC 4 cut(s) 32, 35, 1072, 1253
BlsI GCNGC 4 cut(s) 33, 36, 1073, 1254
Bme1390I CCNGG 4 cut(s) 1222, 1388, 1395, 1437
Bme18I GGWCC 2 cut(s) 58, 1391
BmeRI GACNNNNNGTC 1 cut(s) 390
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 4 cut(s) 58, 269, 270, 1391
BmiI GGNNCC 4 cut(s) 60, 270, 271, 1392
BmrFI CCNGG 4 cut(s) 1222, 1388, 1395, 1437
BmrI ACTGGG 1 cut(s) 212
BmsI GCATC 3 cut(s) 816, 1190, 1513
BmuI ACTGGG 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 770
BpuEI CTTGAG 2 cut(s) 23, 1097
BsaAI YACGTR 2 cut(s) 379, 1267
BsaBI GATNNNNATC 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 7 cut(s) 51, 559, 965, 1220, 1221, 1372, 1387
BsaWI WCCGGW 2 cut(s) 181, 707
BsaXI ACNNNNNCTCC 4 cut(s) 243, 273, 1353, 1383
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1337
Bse118I RCCGGY 4 cut(s) 167, 707, 1204, 1241
Bse1I ACTGG 7 cut(s) 218, 290, 311, 538, 1177, 1281, 1501
Bse3DI GCAATG 1 cut(s) 1108
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 4 cut(s) 1222, 1388, 1395, 1437
BseDI CCNNGG 7 cut(s) 51, 559, 965, 1220, 1221, 1372, 1387
BseGI GGATG 5 cut(s) 138, 685, 781, 985, 1181
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1337
BseMI GCAATG 1 cut(s) 1108
BseMII CTCAG 3 cut(s) 228, 837, 1401
BseNI ACTGG 7 cut(s) 218, 290, 311, 538, 1177, 1281, 1501
BseRI GAGGAG 3 cut(s) 189, 1056, 1625
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 3 cut(s) 43, 46, 1264
BseYI CCCAGC 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 4 cut(s) 151, 228, 271, 1377
BshTI ACCGGT 1 cut(s) 707
BsiEI CGRYCG 1 cut(s) 111
BsiSI CCGG 5 cut(s) 168, 182, 708, 1205, 1242
BslFI GGGAC 4 cut(s) 44, 544, 1062, 1404
BslI CCNNNNNNNGG 5 cut(s) 80, 224, 296, 707, 1337
BsmAI GTCTC 2 cut(s) 373, 435
BsmFI GGGAC 4 cut(s) 44, 544, 1062, 1404
BsmI GAATGC 1 cut(s) 1595
BsnI GGCC 4 cut(s) 151, 228, 271, 1377
Bso31I GGTCTC 1 cut(s) 435
Bsp119I TTCGAA 1 cut(s) 770
Bsp120I GGGCCC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 273, 1548
Bsp1407I TGTACA 1 cut(s) 135
Bsp143I GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
Bsp19I CCATGG 2 cut(s) 51, 559
BspACI CCGC 6 cut(s) 5, 157, 373, 743, 1072, 1255
BspANI GGCC 4 cut(s) 151, 228, 271, 1377
BspCNI CTCAG 3 cut(s) 229, 838, 1402
BspHI TCATGA 2 cut(s) 468, 822
BspLI GGNNCC 4 cut(s) 60, 270, 271, 1392
BspPI GGATC 1 cut(s) 876
BspQI GCTCTTC 1 cut(s) 582
BspT104I TTCGAA 1 cut(s) 770
BspTI CTTAAG 2 cut(s) 395, 446
BspTNI GGTCTC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 1108
BsrFI RCCGGY 4 cut(s) 167, 707, 1204, 1241
BsrGI TGTACA 1 cut(s) 135
BsrI ACTGG 7 cut(s) 218, 290, 311, 538, 1177, 1281, 1501
BssAI RCCGGY 4 cut(s) 167, 707, 1204, 1241
BssECI CCNNGG 7 cut(s) 51, 559, 965, 1220, 1221, 1372, 1387
BssMI GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
BssSI CACGAG 1 cut(s) 581
BssT1I CCWWGG 3 cut(s) 51, 559, 1372
Bst2BI CACGAG 1 cut(s) 581
Bst2UI CCWGG 4 cut(s) 1222, 1388, 1395, 1437
Bst4CI ACNGT 4 cut(s) 637, 969, 1273, 1586
Bst6I CTCTTC 2 cut(s) 148, 582
BstAFI CTTAAG 2 cut(s) 395, 446
BstAPI GCANNNNNTGC 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 135
BstBAI YACGTR 2 cut(s) 379, 1267
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 5 cut(s) 169, 644, 949, 1323, 1593
BstDEI CTNAG 4 cut(s) 237, 488, 846, 1410
BstDSI CCRYGG 3 cut(s) 51, 559, 965
BstF5I GGATG 5 cut(s) 138, 685, 781, 985, 1181
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 6 cut(s) 111, 628, 871, 882, 934, 1318
BstMAI GTCTC 2 cut(s) 373, 435
BstMBI GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 185, 293, 1242
BstNI CCWGG 4 cut(s) 1222, 1388, 1395, 1437
BstNSI RCATGY 4 cut(s) 517, 1071, 1325, 1595
BstSCI CCNGG 4 cut(s) 1220, 1386, 1393, 1435
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 1 cut(s) 273
BstSNI TACGTA 2 cut(s) 379, 1267
BstV1I GCAGC 3 cut(s) 43, 46, 1264
BstX2I RGATCY 2 cut(s) 625, 868
BstYI RGATCY 2 cut(s) 625, 868
BsuRI GGCC 4 cut(s) 151, 228, 271, 1377
BtgI CCRYGG 3 cut(s) 51, 559, 965
BtrI CACGTC 1 cut(s) 392
BtsCI GGATG 5 cut(s) 138, 685, 781, 985, 1181
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 3 cut(s) 304, 678, 1494
Cac8I GCNNGC 5 cut(s) 169, 644, 949, 1323, 1593
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 2 cut(s) 468, 822
CfoI GCGC 1 cut(s) 342
Cfr10I RCCGGY 4 cut(s) 167, 707, 1204, 1241
Cfr13I GGNCC 4 cut(s) 58, 269, 270, 1391
Csp6I GTAC 5 cut(s) 136, 797, 1147, 1264, 1274
CspAI ACCGGT 1 cut(s) 707
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviQI GTAC 5 cut(s) 136, 797, 1147, 1264, 1274
DdeI CTNAG 4 cut(s) 237, 488, 846, 1410
DpnI GATC 6 cut(s) 110, 627, 870, 881, 933, 1317
DpnII GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
DriI GACNNNNNGTC 1 cut(s) 390
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 148, 582
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 2 cut(s) 148, 582
EciI GGCGGA 2 cut(s) 20, 732
Eco105I TACGTA 2 cut(s) 379, 1267
Eco130I CCWWGG 3 cut(s) 51, 559, 1372
Eco147I AGGCCT 1 cut(s) 1377
Eco24I GRGCYC 2 cut(s) 273, 1548
Eco31I GGTCTC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 58, 1391
Eco57I CTGAAG 1 cut(s) 1470
EcoO109I RGGNCCY 2 cut(s) 269, 1391
EcoRII CCWGG 4 cut(s) 1220, 1386, 1393, 1435
EcoRV GATATC 1 cut(s) 302
EcoT14I CCWWGG 3 cut(s) 51, 559, 1372
EcoT22I ATGCAT 3 cut(s) 789, 831, 1597
EcoT38I GRGCYC 2 cut(s) 273, 1548
ErhI CCWWGG 3 cut(s) 51, 559, 1372
FalI AAGNNNNNCTT 2 cut(s) 1107, 1139
FaqI GGGAC 4 cut(s) 44, 544, 1062, 1404
FauI CCCGC 1 cut(s) 380
FauNDI CATATG 1 cut(s) 748
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 4 cut(s) 32, 35, 1072, 1253
FokI GGATG 5 cut(s) 145, 672, 788, 992, 1168
FriOI GRGCYC 2 cut(s) 273, 1548
Fsp4HI GCNGC 4 cut(s) 32, 35, 1072, 1253
FspAI RTGCGCAY 1 cut(s) 341
FspBI CTAG 2 cut(s) 572, 1178
FspI TGCGCA 1 cut(s) 341
GlaI GCGC 1 cut(s) 341
GluI GCNGC 4 cut(s) 32, 35, 1072, 1253
GsaI CCCAGC 1 cut(s) 78
HaeIII GGCC 4 cut(s) 151, 228, 271, 1377
HapII CCGG 5 cut(s) 168, 182, 708, 1205, 1242
HhaI GCGC 1 cut(s) 342
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 2 cut(s) 19, 697
HindII GTYRAC 2 cut(s) 19, 697
HindIII AAGCTT 2 cut(s) 566, 1121
HinfI GANTC 3 cut(s) 104, 1081, 1158
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 5 cut(s) 168, 182, 708, 1205, 1242
HphI GGTGA 7 cut(s) 146, 548, 697, 722, 913, 1237, 1530
Hpy166II GTNNAC 7 cut(s) 19, 103, 697, 720, 959, 1264, 1483
Hpy188I TCNGA 4 cut(s) 995, 1201, 1411, 1450
Hpy188III TCNNGA 9 cut(s) 40, 112, 236, 469, 581, 823, 1085, 1484, 1603
Hpy8I GTNNAC 7 cut(s) 19, 103, 697, 720, 959, 1264, 1483
Hpy99I CGWCG 2 cut(s) 26, 1490
HpyAV CCTTC 3 cut(s) 685, 766, 898
HpyCH4III ACNGT 4 cut(s) 637, 969, 1273, 1586
HpyCH4IV ACGT 3 cut(s) 378, 391, 1266
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 293, 1242
HpyF3I CTNAG 4 cut(s) 237, 488, 846, 1410
HpySE526I ACGT 3 cut(s) 378, 391, 1266
HspAI GCGC 1 cut(s) 340
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 931
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 2 cut(s) 184, 1612
Lsp1109I GCAGC 3 cut(s) 43, 46, 1264
LweI GCATC 3 cut(s) 816, 1190, 1513
MaeI CTAG 2 cut(s) 572, 1178
MaeII ACGT 3 cut(s) 378, 391, 1266
MaeIII GTNAC 7 cut(s) 536, 751, 1267, 1294, 1441, 1536, 1603
MalI GATC 6 cut(s) 110, 627, 870, 881, 933, 1317
MboI GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
MfeI CAATTG 1 cut(s) 1616
MflI RGATCY 2 cut(s) 625, 868
MhlI GDGCHC 2 cut(s) 273, 1548
MluCI AATT 7 cut(s) 425, 840, 858, 1012, 1509, 1562, 1616
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 1318, 1339
Mph1103I ATGCAT 3 cut(s) 789, 831, 1597
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 6 cut(s) 18, 245, 396, 447, 1061, 1469
MspA1I CMGCKG 1 cut(s) 1255
MspCI CTTAAG 2 cut(s) 395, 446
MspI CCGG 5 cut(s) 168, 182, 708, 1205, 1242
MspR9I CCNGG 4 cut(s) 1222, 1388, 1395, 1437
MunI CAATTG 1 cut(s) 1616
Mva1269I GAATGC 1 cut(s) 1595
MvaI CCWGG 4 cut(s) 1222, 1388, 1395, 1437
MwoI GCNNNNNNNGC 3 cut(s) 185, 293, 1242
NaeI GCCGGC 1 cut(s) 169
NcoI CCATGG 2 cut(s) 51, 559
NdeI CATATG 1 cut(s) 748
NdeII GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
NgoMIV GCCGGC 1 cut(s) 167
NlaIV GGNNCC 4 cut(s) 60, 270, 271, 1392
NmeAIII GCCGAG 2 cut(s) 127, 438
NmuCI GTSAC 3 cut(s) 751, 1536, 1603
NsbI TGCGCA 1 cut(s) 341
NsiI ATGCAT 3 cut(s) 789, 831, 1597
NspI RCATGY 4 cut(s) 517, 1071, 1325, 1595
NspV TTCGAA 1 cut(s) 770
PaeI GCATGC 2 cut(s) 1325, 1595
PagI TCATGA 2 cut(s) 468, 822
PasI CCCWGGG 1 cut(s) 1221
PceI AGGCCT 1 cut(s) 1377
PciI ACATGT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 582
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PctI GAATGC 1 cut(s) 1595
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 1081, 1158
PflFI GACNNNGTC 2 cut(s) 818, 1534
PflMI CCANNNNNTGG 2 cut(s) 296, 1337
PinAI ACCGGT 1 cut(s) 707
PkrI GCNGC 4 cut(s) 33, 36, 1073, 1254
Ple19I CGATCG 1 cut(s) 111
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 2 cut(s) 379, 1267
PpuMI RGGWCCY 1 cut(s) 1391
PscI ACATGT 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 1391
Psp6I CCWGG 4 cut(s) 1220, 1386, 1393, 1435
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 4 cut(s) 1220, 1386, 1393, 1435
PspN4I GGNNCC 4 cut(s) 60, 270, 271, 1392
PspOMI GGGCCC 1 cut(s) 269
PspPI GGNCC 4 cut(s) 58, 269, 270, 1391
PspPPI RGGWCCY 1 cut(s) 1391
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 625, 868
PsyI GACNNNGTC 2 cut(s) 818, 1534
PvuI CGATCG 1 cut(s) 111
RsaI GTAC 5 cut(s) 137, 798, 1148, 1265, 1275
RsaNI GTAC 5 cut(s) 136, 797, 1147, 1264, 1274
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 6 cut(s) 18, 245, 396, 447, 1061, 1469
SatI GCNGC 4 cut(s) 32, 35, 1072, 1253
Sau3AI GATC 6 cut(s) 108, 625, 868, 879, 931, 1315
Sau96I GGNCC 4 cut(s) 58, 269, 270, 1391
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 4 cut(s) 1222, 1388, 1395, 1437
SduI GDGCHC 2 cut(s) 273, 1548
SfaNI GCATC 3 cut(s) 816, 1190, 1513
SfcI CTRYAG 1 cut(s) 1209
SfuI TTCGAA 1 cut(s) 770
SgrAI CRCCGGYG 1 cut(s) 707
SinI GGWCC 2 cut(s) 58, 1391
SmlI CTYRAG 4 cut(s) 38, 395, 446, 1076
SmoI CTYRAG 4 cut(s) 38, 395, 446, 1076
SnaBI TACGTA 2 cut(s) 379, 1267
SphI GCATGC 2 cut(s) 1325, 1595
Sse9I AATT 7 cut(s) 425, 840, 858, 1012, 1509, 1562, 1616
SseBI AGGCCT 1 cut(s) 1377
SsiI CCGC 6 cut(s) 5, 157, 373, 743, 1072, 1255
SspI AATATT 2 cut(s) 410, 507
SspMI CTAG 2 cut(s) 572, 1178
StuI AGGCCT 1 cut(s) 1377
StyD4I CCNGG 4 cut(s) 1220, 1386, 1393, 1435
StyI CCWWGG 3 cut(s) 51, 559, 1372
TaaI ACNGT 4 cut(s) 637, 969, 1273, 1586
TaiI ACGT 3 cut(s) 381, 394, 1269
TaqI TCGA 5 cut(s) 12, 107, 111, 770, 1002
TasI AATT 7 cut(s) 425, 840, 858, 1012, 1509, 1562, 1616
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 1074
TfiI GAWTC 2 cut(s) 1081, 1158
Tru1I TTAA 6 cut(s) 18, 245, 396, 447, 1061, 1469
Tru9I TTAA 6 cut(s) 18, 245, 396, 447, 1061, 1469
TscAI CASTG 3 cut(s) 311, 685, 1501
TseFI GTSAC 3 cut(s) 751, 1536, 1603
TseI GCWGC 3 cut(s) 31, 34, 1252
Tsp45I GTSAC 3 cut(s) 751, 1536, 1603
TspRI CASTG 3 cut(s) 311, 685, 1501
Tth111I GACNNNGTC 2 cut(s) 818, 1534
Van91I CCANNNNNTGG 2 cut(s) 296, 1337
Vha464I CTTAAG 2 cut(s) 395, 446
VpaK11BI GGWCC 2 cut(s) 58, 1391
XapI RAATTY 1 cut(s) 425
XceI RCATGY 4 cut(s) 517, 1071, 1325, 1595
XspI CTAG 2 cut(s) 572, 1178
Zsp2I ATGCAT 3 cut(s) 789, 831, 1597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.