pycom02g26080

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008044
Physical Location & Seq
Forward (+)
23933997 .. 23938415
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1311 bp
ATGGCGGAGGTCGAAGTTAACGACGAGAAGCAGCAGCTCAAGAGCAACGCCATGGTGGTCCCTAACCCAAAACCCAACAAGGGTTTTTCTTCAAAAGTGGTGGACTCGATCGAGAAGCTGATTGTGAAGTGGATGCACGACTCTTCTCGGCCTCACCGCTCCCTCGCCGGCAACTTTGCTCCGGTGGCTGATGAAACTCCTCCCACCACCAACCTCCCTGTCATCGGCCATCTTCCTGAGTGCTTAAATGGAGAGTTTGTCAGGGTGGGGCCCAACCCAAAGTTTGCACCAGTTGCTGGATATCACTGGTTTGATGGAGATGGTATGGTTCATGGTATGCGCATAAAAGATGGAAAAGCAACTTATGTCTCCCGCTACGTAAGGACTTCACGTCTTAAGCAAGAACAATATTTTGGAGGTTCTAAATTTATGAAGATTGGAGACCTTAAGGGGTTTCTCGGCTTATTCATGACGAACTTGCAAATGCTAAGAGCAAAACTGAAAATATTAGACATGTCTTATGGTTTTGGGACTGGTAACACGGCTCTCATATATCACCATGGGAAGCTTCTAGCTCTTCACGAGGGGGATAAACCTTATGTCCTCAAAGTTTTGGAAGATGGAGATCTGCAAACGGTTGGCTTGCTGGATTATGATAAGAGATTGACACATTCCTTCACTGCTCATCCAAAGGTTGACCCATTCACCGGTGAGATGTTTATATTTGGCTATTCATCTACTCCGCCATATGTCACTTATAGAGTTATTTCGAAGGATGGTTTCATGCATGACCCTGTACCGATAACAATACCAGACCCCGTCATGATGCATGATTTTGCAATTACTGAGAACTATGCAATTTTCATGGATCTCCCCCTGATCTTCAAACCAAAGGAAATGGTGAAGGAAAAAAAGTTGATATTCACATTTGATCAAACAAAAAAAGCTCGCTTTGGTTTACTTCCACGGTATGCAAAGGATGAGTTGCTAATCAGATGGTTCGAGCTTCCAAATTGCTTCATTTTCCATAATGCTAATGCTTGGGAGGAGGGGGACGAAGTTGTTTTAATCACATGCCGCCTTGAGAATCTTGATTTCGAAAAGCTTGAAAACTTCACAACTGAGCTGTACGAGATGAGATTCAACTTGAACACTGGTCTAGCATCCCAAAAGAAACTATCAGAACCAGCTGTAGATTTCCCCAGGGTGAATGAGAGCTACACCAGCAGGAAGCAGCGTTATGTGTACGGAACCTTACTGGACAACATTGTCAAGGTTACAGGAGTAGCCAAGTTTGATCGTGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

437

Amino Acids

49.73

Weight (kDa)

8.12

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 59 - 433 3.6e-107 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1268
Acc16I TGCGCA 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 296
AccBSI CCGCTC 1 cut(s) 159
AciI CCGC 5 cut(s) 5, 157, 373, 743, 1078
AclWI GGATC 1 cut(s) 876
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 1 cut(s) 425
AfaI GTAC 3 cut(s) 798, 1130, 1247
AfiI CCNNNNNNNGG 4 cut(s) 80, 224, 296, 707
AflII CTTAAG 2 cut(s) 395, 446
AflIII ACRYGT 1 cut(s) 513
AgeI ACCGGT 1 cut(s) 707
AgsI TTSAA 5 cut(s) 93, 886, 1109, 1144, 1150
AhdI GACNNNNNGTC 1 cut(s) 390
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 1 cut(s) 1202
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
Alw26I GTCTC 2 cut(s) 373, 435
AlwI GGATC 1 cut(s) 876
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 3 cut(s) 149, 226, 269
ApaI GGGCCC 1 cut(s) 273
ApeKI GCWGC 3 cut(s) 31, 34, 1234
ApoI RAATTY 1 cut(s) 425
AsiGI ACCGGT 1 cut(s) 707
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 3 cut(s) 58, 269, 270
AsuHPI GGTGA 6 cut(s) 146, 548, 697, 722, 913, 1219
AsuII TTCGAA 2 cut(s) 770, 1098
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 273
BauI CACGAG 1 cut(s) 581
BbvI GCAGC 3 cut(s) 43, 46, 1246
BccI CCATC 7 cut(s) 237, 308, 314, 344, 614, 770, 990
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 1 cut(s) 1204
BclI TGATCA 1 cut(s) 931
BcoDI GTCTC 2 cut(s) 373, 435
BfaI CTAG 2 cut(s) 572, 1160
BfmI CTRYAG 1 cut(s) 1191
BfrI CTTAAG 2 cut(s) 395, 446
BglII AGATCT 1 cut(s) 625
BisI GCNGC 4 cut(s) 32, 35, 1078, 1235
BlsI GCNGC 4 cut(s) 33, 36, 1079, 1236
Bme1390I CCNGG 1 cut(s) 1204
Bme18I GGWCC 1 cut(s) 58
BmeRI GACNNNNNGTC 1 cut(s) 390
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 3 cut(s) 58, 269, 270
BmiI GGNNCC 4 cut(s) 60, 270, 271, 1252
BmrFI CCNGG 1 cut(s) 1204
BmsI GCATC 3 cut(s) 123, 816, 1172
Bpu14I TTCGAA 2 cut(s) 770, 1098
BpuEI CTTGAG 2 cut(s) 23, 1103
BsaAI YACGTR 1 cut(s) 379
BsaBI GATNNNNATC 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 435
BsaJI CCNNGG 5 cut(s) 51, 559, 965, 1202, 1203
BsaWI WCCGGW 2 cut(s) 181, 707
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 224, 296, 707
Bse118I RCCGGY 2 cut(s) 167, 707
Bse1I ACTGG 5 cut(s) 290, 311, 538, 1159, 1263
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 1 cut(s) 1204
BseDI CCNNGG 5 cut(s) 51, 559, 965, 1202, 1203
BseGI GGATG 5 cut(s) 138, 685, 781, 985, 1163
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 4 cut(s) 80, 224, 296, 707
BseMII CTCAG 3 cut(s) 228, 837, 1113
BseNI ACTGG 5 cut(s) 290, 311, 538, 1159, 1263
BseRI GAGGAG 2 cut(s) 189, 1061
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 3 cut(s) 43, 46, 1246
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 151, 228, 271
BshTI ACCGGT 1 cut(s) 707
BsiEI CGRYCG 1 cut(s) 111
BsiSI CCGG 3 cut(s) 168, 182, 708
BslFI GGGAC 3 cut(s) 44, 544, 1067
BslI CCNNNNNNNGG 4 cut(s) 80, 224, 296, 707
BsmAI GTCTC 2 cut(s) 373, 435
BsmFI GGGAC 3 cut(s) 44, 544, 1067
BsnI GGCC 3 cut(s) 151, 228, 271
Bso31I GGTCTC 1 cut(s) 435
Bsp119I TTCGAA 2 cut(s) 770, 1098
Bsp120I GGGCCC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
Bsp19I CCATGG 2 cut(s) 51, 559
BspACI CCGC 5 cut(s) 5, 157, 373, 743, 1078
BspANI GGCC 3 cut(s) 151, 228, 271
BspCNI CTCAG 3 cut(s) 229, 838, 1114
BspHI TCATGA 2 cut(s) 468, 822
BspLI GGNNCC 4 cut(s) 60, 270, 271, 1252
BspPI GGATC 1 cut(s) 876
BspQI GCTCTTC 1 cut(s) 582
BspT104I TTCGAA 2 cut(s) 770, 1098
BspTI CTTAAG 2 cut(s) 395, 446
BspTNI GGTCTC 1 cut(s) 435
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 167, 707
BsrI ACTGG 5 cut(s) 290, 311, 538, 1159, 1263
BssAI RCCGGY 2 cut(s) 167, 707
BssECI CCNNGG 5 cut(s) 51, 559, 965, 1202, 1203
BssMI GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
BssSI CACGAG 1 cut(s) 581
BssT1I CCWWGG 2 cut(s) 51, 559
Bst2BI CACGAG 1 cut(s) 581
Bst2UI CCWGG 1 cut(s) 1204
Bst4CI ACNGT 2 cut(s) 637, 969
Bst6I CTCTTC 2 cut(s) 148, 582
BstAFI CTTAAG 2 cut(s) 395, 446
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 2 cut(s) 770, 1098
BstC8I GCNNGC 4 cut(s) 169, 644, 949, 1306
BstDEI CTNAG 4 cut(s) 237, 488, 846, 1122
BstDSI CCRYGG 3 cut(s) 51, 559, 965
BstF5I GGATG 5 cut(s) 138, 685, 781, 985, 1163
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 6 cut(s) 111, 628, 871, 882, 934, 1300
BstMAI GTCTC 2 cut(s) 373, 435
BstMBI GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 185, 293, 1224
BstNI CCWGG 1 cut(s) 1204
BstNSI RCATGY 3 cut(s) 517, 1077, 1308
BstSCI CCNGG 1 cut(s) 1202
BstSFI CTRYAG 1 cut(s) 1191
BstSLI GKGCMC 1 cut(s) 273
BstSNI TACGTA 1 cut(s) 379
BstV1I GCAGC 3 cut(s) 43, 46, 1246
BstX2I RGATCY 2 cut(s) 625, 868
BstYI RGATCY 2 cut(s) 625, 868
BsuRI GGCC 3 cut(s) 151, 228, 271
BtgI CCRYGG 3 cut(s) 51, 559, 965
BtrI CACGTC 1 cut(s) 392
BtsCI GGATG 5 cut(s) 138, 685, 781, 985, 1163
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 3 cut(s) 304, 678, 1152
Cac8I GCNNGC 4 cut(s) 169, 644, 949, 1306
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 2 cut(s) 468, 822
CfoI GCGC 1 cut(s) 342
Cfr10I RCCGGY 2 cut(s) 167, 707
Cfr13I GGNCC 3 cut(s) 58, 269, 270
Csp6I GTAC 3 cut(s) 797, 1129, 1246
CspAI ACCGGT 1 cut(s) 707
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviQI GTAC 3 cut(s) 797, 1129, 1246
DdeI CTNAG 4 cut(s) 237, 488, 846, 1122
DpnI GATC 6 cut(s) 110, 627, 870, 881, 933, 1299
DpnII GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
DrdI GACNNNNNNGTC 1 cut(s) 1268
DriI GACNNNNNGTC 1 cut(s) 390
DseDI GACNNNNNNGTC 1 cut(s) 1268
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 148, 582
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 2 cut(s) 148, 582
EciI GGCGGA 2 cut(s) 20, 732
Eco105I TACGTA 1 cut(s) 379
Eco130I CCWWGG 2 cut(s) 51, 559
Eco24I GRGCYC 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 1202
EcoRV GATATC 1 cut(s) 302
EcoT14I CCWWGG 2 cut(s) 51, 559
EcoT22I ATGCAT 2 cut(s) 789, 831
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 51, 559
FaqI GGGAC 3 cut(s) 44, 544, 1067
FauI CCCGC 1 cut(s) 380
FauNDI CATATG 1 cut(s) 748
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 4 cut(s) 32, 35, 1078, 1235
FokI GGATG 5 cut(s) 145, 672, 788, 992, 1150
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 4 cut(s) 32, 35, 1078, 1235
FspAI RTGCGCAY 1 cut(s) 341
FspBI CTAG 2 cut(s) 572, 1160
FspI TGCGCA 1 cut(s) 341
GlaI GCGC 1 cut(s) 341
GluI GCNGC 4 cut(s) 32, 35, 1078, 1235
HaeIII GGCC 3 cut(s) 151, 228, 271
HapII CCGG 3 cut(s) 168, 182, 708
HhaI GCGC 1 cut(s) 342
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 2 cut(s) 19, 697
HindII GTYRAC 2 cut(s) 19, 697
HindIII AAGCTT 2 cut(s) 566, 1103
HinfI GANTC 4 cut(s) 104, 140, 1087, 1140
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 3 cut(s) 168, 182, 708
HphI GGTGA 6 cut(s) 146, 548, 697, 722, 913, 1219
Hpy166II GTNNAC 5 cut(s) 19, 103, 697, 959, 1246
Hpy188I TCNGA 2 cut(s) 995, 1183
Hpy188III TCNNGA 7 cut(s) 40, 112, 236, 469, 581, 823, 1091
Hpy8I GTNNAC 5 cut(s) 19, 103, 697, 959, 1246
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 685, 766, 898
HpyCH4III ACNGT 2 cut(s) 637, 969
HpyCH4IV ACGT 2 cut(s) 378, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 293, 1224
HpyF3I CTNAG 4 cut(s) 237, 488, 846, 1122
HpySE526I ACGT 2 cut(s) 378, 391
HspAI GCGC 1 cut(s) 340
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 931
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 2 cut(s) 164, 184
Lsp1109I GCAGC 3 cut(s) 43, 46, 1246
LweI GCATC 3 cut(s) 123, 816, 1172
MaeI CTAG 2 cut(s) 572, 1160
MaeII ACGT 2 cut(s) 378, 391
MaeIII GTNAC 3 cut(s) 536, 751, 1276
MalI GATC 6 cut(s) 110, 627, 870, 881, 933, 1299
MbiI CCGCTC 1 cut(s) 159
MboI GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
MboII GAAGA 7 cut(s) 81, 135, 224, 445, 569, 629, 874
MflI RGATCY 2 cut(s) 625, 868
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 4 cut(s) 425, 840, 858, 1012
MlyI GAGTC 2 cut(s) 98, 134
MnlI CCTC 9 cut(s) 162, 173, 210, 224, 410, 577, 614, 1039, 1042
Mph1103I ATGCAT 2 cut(s) 789, 831
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 5 cut(s) 18, 245, 396, 447, 1067
MspA1I CMGCKG 1 cut(s) 1190
MspCI CTTAAG 2 cut(s) 395, 446
MspI CCGG 3 cut(s) 168, 182, 708
MspR9I CCNGG 1 cut(s) 1204
MvaI CCWGG 1 cut(s) 1204
MwoI GCNNNNNNNGC 3 cut(s) 185, 293, 1224
NaeI GCCGGC 1 cut(s) 169
NcoI CCATGG 2 cut(s) 51, 559
NdeI CATATG 1 cut(s) 748
NdeII GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
NgoMIV GCCGGC 1 cut(s) 167
NlaIV GGNNCC 4 cut(s) 60, 270, 271, 1252
NmeAIII GCCGAG 2 cut(s) 127, 438
NmuCI GTSAC 1 cut(s) 751
NsbI TGCGCA 1 cut(s) 341
NsiI ATGCAT 2 cut(s) 789, 831
NspI RCATGY 3 cut(s) 517, 1077, 1308
NspV TTCGAA 2 cut(s) 770, 1098
PaeI GCATGC 1 cut(s) 1308
PagI TCATGA 2 cut(s) 468, 822
PasI CCCWGGG 1 cut(s) 1203
PciI ACATGT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 582
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 1087, 1140
PflFI GACNNNGTC 1 cut(s) 818
PflMI CCANNNNNTGG 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 707
PkrI GCNGC 4 cut(s) 33, 36, 1079, 1236
Ple19I CGATCG 1 cut(s) 111
PleI GAGTC 2 cut(s) 98, 134
PpsI GAGTC 2 cut(s) 98, 134
Ppu21I YACGTR 1 cut(s) 379
PscI ACATGT 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 1202
PspGI CCWGG 1 cut(s) 1202
PspN4I GGNNCC 4 cut(s) 60, 270, 271, 1252
PspOMI GGGCCC 1 cut(s) 269
PspPI GGNCC 3 cut(s) 58, 269, 270
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 2 cut(s) 625, 868
PsyI GACNNNGTC 1 cut(s) 818
PvuI CGATCG 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 1190
RsaI GTAC 3 cut(s) 798, 1130, 1247
RsaNI GTAC 3 cut(s) 797, 1129, 1246
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 5 cut(s) 18, 245, 396, 447, 1067
SatI GCNGC 4 cut(s) 32, 35, 1078, 1235
Sau3AI GATC 6 cut(s) 108, 625, 868, 879, 931, 1297
Sau96I GGNCC 3 cut(s) 58, 269, 270
SchI GAGTC 2 cut(s) 98, 134
ScrFI CCNGG 1 cut(s) 1204
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 3 cut(s) 123, 816, 1172
SfcI CTRYAG 1 cut(s) 1191
SfuI TTCGAA 2 cut(s) 770, 1098
SgrAI CRCCGGYG 1 cut(s) 707
SinI GGWCC 1 cut(s) 58
SmlI CTYRAG 4 cut(s) 38, 395, 446, 1082
SmoI CTYRAG 4 cut(s) 38, 395, 446, 1082
SnaBI TACGTA 1 cut(s) 379
SphI GCATGC 1 cut(s) 1308
Sse9I AATT 4 cut(s) 425, 840, 858, 1012
SsiI CCGC 5 cut(s) 5, 157, 373, 743, 1078
SspI AATATT 2 cut(s) 410, 507
SspMI CTAG 2 cut(s) 572, 1160
StyD4I CCNGG 1 cut(s) 1202
StyI CCWWGG 2 cut(s) 51, 559
TaaI ACNGT 2 cut(s) 637, 969
TaiI ACGT 2 cut(s) 381, 394
TaqI TCGA 6 cut(s) 12, 107, 111, 770, 1002, 1098
TasI AATT 4 cut(s) 425, 840, 858, 1012
TauI GCSGC 1 cut(s) 1080
TfiI GAWTC 2 cut(s) 1087, 1140
Tru1I TTAA 5 cut(s) 18, 245, 396, 447, 1067
Tru9I TTAA 5 cut(s) 18, 245, 396, 447, 1067
TscAI CASTG 3 cut(s) 311, 685, 1159
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 3 cut(s) 31, 34, 1234
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 8 cut(s) 207, 320, 446, 457, 723, 772, 853, 1009
TspGWI ACGGA 1 cut(s) 1263
TspRI CASTG 3 cut(s) 311, 685, 1159
Tth111I GACNNNGTC 1 cut(s) 818
Van91I CCANNNNNTGG 1 cut(s) 296
Vha464I CTTAAG 2 cut(s) 395, 446
VpaK11BI GGWCC 1 cut(s) 58
XapI RAATTY 1 cut(s) 425
XceI RCATGY 3 cut(s) 517, 1077, 1308
XspI CTAG 2 cut(s) 572, 1160
Zsp2I ATGCAT 2 cut(s) 789, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.