pycom03g00100

TATA-binding protein-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007901
Physical Location & Seq
Forward (+)
74613 .. 94335
19723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4749 bp
ATGGATGTGCTGAAAAGGCTCTCTGAAAATAGTGATATGATGAACTTGGTTAAACTGGCTAGGCATTCATGGCTTAAAAATTGTGAATTTCTTCAAGATTGTGCAATTCGTTTCTTGTGTGTCTTGTCACTAGACCGTTTTGGAGATTATGTCTCTGATCACGTAGTTGCTCCAGTACGTGAAACCTGTGCTCAAGCATTGGGTGTTGTGTTCAAATACATGCATCCCACTTTAGTTCATGAAACACTGAACATCTTGCTGAAGATGCAGTGTAGACCAGAATGGGAAATTCGTCATGGAAGCCTATTGGGTATTAAGTATCTAGTTGCCGTGCGGCAGGAGATGCTTCATGATTTGCTTGACCGGGTCCTGTCTGCATGTAAAGCTGGCCTGGAGGACCCCGATGATGATGTTCGAGCAGTAGCTGCAGATGCTTTGATACCTACCTCAGCTGCTATTGTTGCTTTAAAGGGTGAAACTTTGCATTCTATAGTGATGTTGGTTTGGGATATATTACTTGATTTGGATGATTTAAGTCCATCCACTAGCAGCGTAATGAATCTTTTGGCGGAAATCTATTCCCAGGAAGACATGATTCCTAAGATTTTTGAGGCATTGACATCAAAAGAGATTGATTTGAATGAATTGGGTAGCATTGATGATACGGGAGAAGGATTAATTTCGCAAGATAATCCTTTCATGTTATCAACGCTTGCACCGCGATTGTGGCCCTTTATGAGGCATAGTATCACATCAGTTCGTTATTCAGCTATCCGCACGTTGGAACGACTACTTGAAGCAGGATACAAAAGGAGTCTCTCTGAGTCTTCGAGTACTTCATTTTGGCCTTCTTTTATCTTGGGTGATACCCTTCGGATTGTTTTTCAGAATTTACTGCTTGAATCAAATGACGAAATTTTGAAACGTTCAGAGAGAGTTTGGGGGCTCCTTGTTCAGTGTCCATTAGGTGACTTGGAAATTGCTGCAAGGTCATATATGTCCTCTTGGATAGGGCTTGCAACTACACCGTATGGATCAGCATTACATTCTACAAAAATGTTTTGGCCTGTTGCTCTCCCACGAAAGAGTCATTTTAAAGCAGCAGCTAAAATGAGAGCTGTGAATCTTGTAAATGAGTCTTGTAGAAACATCGGGTTGGAATCTTCGAATGGATCCATTCCAGAAAAAGGTGGAGATGCTTCAACCAATAATGTTCAGATAGTTGTTGGTGCGGATGTTGAAATGTCAGTTACGCATACCAGAGTGGTTACTGCAGCAGCATTGGGAGTTTTTGCTTCTAGATTGCACGAAACTTCAATGCAGTATGTAATTGATCCACTGACTAAAGCATTTACCTCTTTATCTGGTGTCCAGCGGCAGGTGGCATCTATGGTTCTTCTTTCTTGGTTCAAAGAGATTAAAAGTGCAGATGTGTTTGAAAATGCTGGAGTTATGCCAGGTTTTCTGAACCATCTCAAACATTTGATGTTGGATTTCTTGGCATGCTCTGAACCTGCTTTCCCTACAAACGATCCATGTCTTCCTTATGCCGAGCTTTCAAGAACGTATTGTAAGATGCGTTGTGAGGCTAGTCAGTTGTTAAATGCTATTCAATCATCGGGTATGTTTCAGAGTTTGTTGTCTACTACCAAAATTGACTTGGAAAGGTTGAACGTGGACAATGCAATTAATTTTGCATCGAAACTTCCAATGTTGTGTAACAATGTTGAGGGAAATGATTCTTTGGGAAGGCACATTGTTGACGATATAGAGTCAGCAAAACAACGACTTCTGACAACTTGTGGATATTTAAAATGTGTTCAGAGCAATCTGCATGTTACTGTCTCGTCTCTTGTTGCTGCTTCTATTGTTTGGATGTCAGAGCTTCCTGAACGACTAAATCCAATTATTTTGCCTTTGATGGCTTCTATCAAAAGAGAACAGGAGGAAATACTACAAGAGAAGGCAGCTGAGGCACTTGCAGAGCTTATTTCTCATTGTATTTCACGTAGCCGAAGCCCAAATGATAAGTTAATTAAGAATATATGTAATTTAACATGCGTGGATCCTAGAGAAACACCTCAAGCTGCAGTCATCTGTTCCATAGATATTATTGATGATCAGGATCTCCTATCTTTTGGGACAAATACTGGTAAGCAGAAATCAAAGGTCATTCTGGCTGGTAACGAAGACCGCTCAAAGGTTGAAGGCTTTATTAGCAGACGAGGCTCTGAACTTGCCTTGAGGCATCTTTGTGTGAAGTTTGGTGCTTCATTATTTGATAAGCTTCCAAAACTATGGGATTGCCTCACTGAAGTGCTTAAGCCTAGTAGTATTGAGCCCCTTAATCCTGCAGATGAAAAGAAAATTACACAAACTCTAGAATCTGTGAAGGATCCCCAGATCTTGATCAATAATATCCAGGTAGTACGTTCTATTGCTCCAATGCTAAATGAGGATTTGAAACCAAAACTACTCACCCTTCTTCCATACATATTTAAGTGTGTTCGCCATTCCCATGTTGCTGTTAGATTGGCTTCCTCTAGATGCATCACCACAATGGCCAAGTCAATGGAAATGCATGTGATGGGAGCTGTAATTGAAAATGTCATACCCATGTTAGGGGATATGACATCTGTTGATGCCAGACAAGGTGCAGGCATGGTTATTAGTTTGCTTGTCCAAGGACTGGGTGTGGAGCTGGTTCCATATGCTCCTTTCTTAGTTGTCCCTCTTCTAAGGTGTATGAGTGATTGTGATCAGTCTGTCAGACAGAGTGTGACTCACAGCTTTGCTGCTCTTGTACCTCTTCTTCCACTCGCACGGGGCCTCCCTCCACCTGTTGGACTAACTGAAGGTTTCTCCAGGAGTGCAGAAGATGCAACATTTCTGGAGCAACTACTTGACAACTCCCATATTGATGATTACAAGCTTTGTACTGAACTGAAAGTGACACTGAGAAGGTATCAACAGGAAGGCATAAATTGGTTGGCCTTTTTAAAACGTTTCAAGCTTCATGGAATCTTATGTGATGATATGGGTCTTGGTAAGACACTTCAAGCATCTGCTATTGTGGCTTCTGATATAGTTGAGCATCAAACTTTGAATGATGGCAATCCTCCCTCATCTTTAATTATTTGCCCATCAACACTTGTTGGACATTGGGCCTTTGAGATAGAGAAGTACATTGATCTTTCCATAATATCTACACTCCAATATGTTGGTTCTGCGCAAGAGCGCATTTCTCTTCGAGAACATTTTGAAAAGCATAATGTGATAATAACATCATATGATGTGGTTCGCAAAGATGTTGATTATCTTGGGAAGCTTCTCTGGAACTATTGTATCTTAGATGAAGGACATGTGATCAAGAATGCAAAGTCGAAAATTACACTTTCAGTGAAGCAATTGAAAGCCCAGCATCGTTTGATATTAAGTGGAACACCAATCCAGAATAACGTCATGGATTTGTGGTCCCTTTTTGATTTCCTAATGCCCGGCTTTCTTGGAACAGAGAGGCAGTTCCAAGCCACTTATGGAAAACCACTGGTGGCAGCCAGAGATCCTAAGTGCTCTGCCAAGGATGCTGAAGCAGGTGCACTTGCAATGGAAGCATTGCACAAGCAGAGGTCATGCCTTTCCTCCTCCGTCGAACAAAAGATGAAGTCTTGTCTGATCGTCACCGAGAAAATTATTCAGGACAGGTTCTGTGACCTGAGCCCTGTACAATTGAAATTGTATGAACAGTTTTCTGGGTCACATGTGAGACAAGAGATCTCAAGTATTGTAAAACAAAATGAGTCAGCAGATACAGGAGGGCGTAGTGAATCACCTAAAGCTTCATCCCATGTTTTTCAGGCACTTCAGTACTTGCTTAAACTTTGCAGTCACCCATTGCTTGTTCTTGGAGAAAAGGTCCCTGATTCAATTGCATGCCTTTTGTCCGAGCTACTACCGGGAGGCTTTGATACAGTTTCAGAATTGCACAAACCATACCACTCTCCCAAACTAGTTGCTCTTCAGGAGATTCTTGAAGAGTGTGGAATAGGTGTTGATGCTTCTAGTTCTGAAGGTGCCATAAATGTGGGGCAGCACAGAGTCTTGATATTTGCTCAACATAAAGCATTTCTGGACTTAATAGAAAGAGACTTGTTTCTCACTCATATGAAGAGTGTAACCTACTTGCGGCTGGATGGGTCAGTTGAACCTGAAAAACGTTTTGATATTGTCAAAGCTTTCAATTCGGACCCTACAATCGATGTTCTGCTACTCACAACCCATGTTGGTGGGCTGGGGTTGAACCTGACATCTGCAGACACACTCGTTTTCATGGAGCATGACTGGAACCCCATGCGGGACCATCAGGCAATGGACAGAGCACACAGATTAGGTCAGAAAAAAGTTGTCAATGTCCATCGCCTTATTATGCGTGGCACTCTCGAAGAGAAAGTTATGAGCTTGCAAAAGTTTAAGCTTTCAGTAGCTAATGCAGTCATTAATGCTGAAAATGCTAGTATGAAAACCATGAACACAGACCAACTGCTTGATCTATTTGCTACTGCAGAAACATCTAAAAAGGGAACTTCTATATTAAAGCATCCTGATGGAAAATTTGATGGAGATATGAAATTAATGGGCACTGGAAAGGGGTTGAAAGCAATTCTTGGTGGGCTGGAGGAGTTGTGGGATCAATCACAGTACACCGAAGAATACAATCTCAGTAACTTCTTGGCAAAGCTTGACGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0048509 GO:0048518 GO:0050789 GO:0050793 GO:0051094 GO:0065007 GO:1902183 GO:1902185
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3535 HEAT HEAT_EZ Helicase_C SNF2_N

Protein Analysis

1583

Amino Acids

175.63

Weight (kDa)

6.01

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3535 PF12054 313 - 769 3.1e-97 Domain of unknown function (DUF3535)
HEAT_ECM29 PF24492 849 - 938 6.5e-06 Proteasome adapter and scaffold protein ECM29, HEAT-repeat domain
SNF2-rel_dom PF00176 989 - 1298 1.3e-61 SNF2-related domain
Helicase_C PF00271 1364 - 1464 1.7e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1369, 3584
Acc16I TGCGCA 1 cut(s) 3231
Acc36I ACCTGC 3 cut(s) 1369, 1522, 3584
AccB1I GGYRCC 1 cut(s) 4073
AccB7I CCANNNNNTGG 2 cut(s) 2689, 2843
AccBSI CCGCTC 1 cut(s) 2196
AccI GTMKAC 2 cut(s) 274, 1643
AccII CGCG 1 cut(s) 721
AciI CCGC 9 cut(s) 334, 569, 719, 775, 1232, 1375, 2194, 4186, 4354
AclI AACGTT 3 cut(s) 925, 3004, 4216
AcoI YGGCCR 1 cut(s) 2562
AcsI RAATTY 5 cut(s) 86, 288, 889, 915, 4610
AcuI CTGAAG 7 cut(s) 281, 2334, 2874, 3609, 3848, 4004, 4089
AfaI GTAC 9 cut(s) 177, 835, 2430, 2805, 2938, 3185, 3726, 3869, 4700
AflII CTTAAG 1 cut(s) 2321
AflIII ACRYGT 2 cut(s) 3361, 3760
AhlI ACTAGT 1 cut(s) 4009
AjnI CCWGG 5 cut(s) 390, 582, 1456, 2421, 2864
AleI CACNNNNGTG 1 cut(s) 2315
AloI GAACNNNNNNTCC 2 cut(s) 1503, 1535
Alw21I GWGCWC 4 cut(s) 193, 3575, 3601, 4381
Alw26I GTCTC 6 cut(s) 157, 821, 1849, 1854, 3760, 4140
Alw44I GTGCAC 1 cut(s) 3597
AlwNI CAGNNNCTG 2 cut(s) 425, 3708
AoxI GGCC 8 cut(s) 388, 728, 845, 1064, 2562, 2827, 2991, 3165
ApaLI GTGCAC 1 cut(s) 3597
ApoI RAATTY 5 cut(s) 86, 288, 889, 915, 4610
AseI ATTAAT 4 cut(s) 677, 1689, 4497, 4631
Asp700I GAANNNNTTC 2 cut(s) 90, 1738
AspLEI GCGC 2 cut(s) 3232, 3240
AspS9I GGNCC 9 cut(s) 367, 397, 729, 2827, 3165, 3474, 3916, 4246, 4357
AsuC2I CCSGG 3 cut(s) 365, 3498, 3957
AsuHPI GGTGA 8 cut(s) 485, 875, 980, 2470, 2545, 3673, 3822, 3881
AsuII TTCGAA 1 cut(s) 1166
AvaII GGWCC 6 cut(s) 367, 397, 3474, 3916, 4246, 4357
BaeGI GKGCMC 2 cut(s) 3601, 4640
BaeI ACNNNNGTAYC 2 cut(s) 3328, 3361
BalI TGGCCA 1 cut(s) 2564
BamHI GGATCC 3 cut(s) 1172, 2065, 2395
BanI GGYRCC 1 cut(s) 4073
BanII GRGCYC 3 cut(s) 948, 2343, 3722
BarI GAAGNNNNNNTAC 2 cut(s) 2796, 2828
BbsI GAAGAC 4 cut(s) 594, 819, 1532, 2196
Bbv12I GWGCWC 4 cut(s) 193, 3575, 3601, 4381
BbvCI CCTCAGC 2 cut(s) 448, 1971
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 5 cut(s) 392, 584, 1458, 2423, 2866
BciVI GTATCC 1 cut(s) 797
BclI TGATCA 5 cut(s) 157, 2119, 2409, 2758, 3366
BcnI CCSGG 3 cut(s) 365, 3498, 3957
BcoDI GTCTC 6 cut(s) 157, 821, 1849, 1854, 3760, 4140
BcuI ACTAGT 1 cut(s) 4009
BfmI CTRYAG 7 cut(s) 426, 489, 1272, 2088, 2352, 4311, 4560
BfrI CTTAAG 1 cut(s) 2321
BfuAI ACCTGC 3 cut(s) 1369, 1522, 3584
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 2 cut(s) 2403, 3774
BmcAI AGTACT 2 cut(s) 835, 3869
Bme1390I CCNGG 8 cut(s) 365, 392, 584, 1458, 2423, 2866, 3498, 3957
Bme18I GGWCC 6 cut(s) 367, 397, 3474, 3916, 4246, 4357
BmgT120I GGNCC 9 cut(s) 367, 397, 729, 2827, 3165, 3474, 3916, 4246, 4357
BmrFI CCNGG 8 cut(s) 365, 392, 584, 1458, 2423, 2866, 3498, 3957
BmrI ACTGGG 1 cut(s) 2699
BmuI ACTGGG 1 cut(s) 2699
BpiI GAAGAC 4 cut(s) 594, 819, 1532, 2196
BplI GAGNNNNNCTC 2 cut(s) 2767, 2799
BpmI CTGGAG 6 cut(s) 156, 413, 1467, 2848, 2912, 4694
Bpu10I CCTNAGC 3 cut(s) 448, 1971, 3716
Bpu14I TTCGAA 1 cut(s) 1166
BpuEI CTTGAG 4 cut(s) 177, 2067, 2263, 3763
BpuMI CCSGG 3 cut(s) 365, 3498, 3957
Bsa29I ATCGAT 1 cut(s) 4257
BsaAI YACGTR 3 cut(s) 163, 179, 2009
BsaBI GATNNNNATC 4 cut(s) 2124, 2408, 2757, 3114
BsaJI CCNNGG 3 cut(s) 582, 2683, 3579
BsaXI ACNNNNNCTCC 8 cut(s) 135, 165, 2814, 2844, 3900, 3930, 4667, 4697
Bse1I ACTGG 7 cut(s) 60, 173, 2155, 2694, 3552, 4346, 4645
Bse3DI GCAATG 4 cut(s) 3612, 3614, 3893, 4374
Bse8I GATNNNNATC 4 cut(s) 2124, 2408, 2757, 3114
BseBI CCWGG 5 cut(s) 392, 584, 1458, 2423, 2866
BseCI ATCGAT 1 cut(s) 4257
BseDI CCNNGG 3 cut(s) 582, 2683, 3579
BseJI GATNNNNATC 4 cut(s) 2124, 2408, 2757, 3114
BseMI GCAATG 4 cut(s) 3612, 3614, 3893, 4374
BseMII CTCAG 6 cut(s) 462, 813, 1962, 2948, 3707, 4732
BseNI ACTGG 7 cut(s) 60, 173, 2155, 2694, 3552, 4346, 4645
BseRI GAGGAG 2 cut(s) 3634, 4691
BseSI GKGCMC 2 cut(s) 3601, 4640
BseYI CCCAGC 2 cut(s) 3417, 4291
BsgI GTGCAG 3 cut(s) 1446, 2676, 2892
Bsh1236I CGCG 1 cut(s) 721
BshFI GGCC 8 cut(s) 390, 730, 847, 1066, 2564, 2829, 2993, 3167
BshNI GGYRCC 1 cut(s) 4073
BshVI ATCGAT 1 cut(s) 4257
BsiHKAI GWGCWC 4 cut(s) 193, 3575, 3601, 4381
BsiSI CCGG 3 cut(s) 364, 3498, 3956
BslFI GGGAC 5 cut(s) 2155, 2714, 3460, 3902, 4370
BsmAI GTCTC 6 cut(s) 157, 821, 1849, 1854, 3760, 4140
BsmBI CGTCTC 1 cut(s) 1854
BsmFI GGGAC 5 cut(s) 2155, 2714, 3460, 3902, 4370
BsmI GAATGC 3 cut(s) 64, 484, 3379
BsnI GGCC 8 cut(s) 390, 730, 847, 1066, 2564, 2829, 2993, 3167
Bsp119I TTCGAA 1 cut(s) 1166
Bsp1286I GDGCHC 8 cut(s) 193, 948, 2343, 3575, 3601, 3722, 4381, 4640
Bsp1407I TGTACA 1 cut(s) 3724
BspACI CCGC 9 cut(s) 334, 569, 719, 775, 1232, 1375, 2194, 4186, 4354
BspANI GGCC 8 cut(s) 390, 730, 847, 1066, 2564, 2829, 2993, 3167
BspCNI CTCAG 6 cut(s) 461, 814, 1963, 2949, 3708, 4731
BspDI ATCGAT 1 cut(s) 4257
BspFNI CGCG 1 cut(s) 721
BspHI TCATGA 2 cut(s) 238, 349
BspMAI CTGCAG 6 cut(s) 430, 1276, 2092, 2356, 4315, 4564
BspMI ACCTGC 3 cut(s) 1369, 1522, 3584
BspQI GCTCTTC 1 cut(s) 4023
BspT104I TTCGAA 1 cut(s) 1166
BspT107I GGYRCC 1 cut(s) 4073
BspTI CTTAAG 1 cut(s) 2321
BsrBI CCGCTC 1 cut(s) 2196
BsrDI GCAATG 4 cut(s) 3612, 3614, 3893, 4374
BsrGI TGTACA 1 cut(s) 3724
BsrI ACTGG 7 cut(s) 60, 173, 2155, 2694, 3552, 4346, 4645
BssECI CCNNGG 3 cut(s) 582, 2683, 3579
BssT1I CCWWGG 2 cut(s) 2683, 3579
Bst2UI CCWGG 5 cut(s) 392, 584, 1458, 2423, 2866
Bst4CI ACNGT 7 cut(s) 137, 1029, 1843, 3747, 3973, 4698, 4745
Bst6I CTCTTC 7 cut(s) 2739, 2814, 3252, 4023, 4029, 4163, 4437
BstAFI CTTAAG 1 cut(s) 2321
BstAPI GCANNNNNTGC 3 cut(s) 343, 425, 2879
BstAUI TGTACA 1 cut(s) 3724
BstBAI YACGTR 3 cut(s) 163, 179, 2009
BstBI TTCGAA 1 cut(s) 1166
BstC8I GCNNGC 7 cut(s) 388, 714, 1017, 1504, 2659, 3934, 4460
BstENI CCTNNNNNAGG 1 cut(s) 736
BstFNI CGCG 1 cut(s) 721
BstHHI GCGC 2 cut(s) 3232, 3240
BstMAI GTCTC 6 cut(s) 157, 821, 1849, 1854, 3760, 4140
BstNI CCWGG 5 cut(s) 392, 584, 1458, 2423, 2866
BstNSI RCATGY 9 cut(s) 223, 381, 1506, 1838, 2061, 2585, 3365, 3764, 3936
BstSCI CCNGG 8 cut(s) 363, 390, 582, 1456, 2421, 2864, 3496, 3955
BstSFI CTRYAG 7 cut(s) 426, 489, 1272, 2088, 2352, 4311, 4560
BstSLI GKGCMC 2 cut(s) 3601, 4640
BstUI CGCG 1 cut(s) 721
BstV2I GAAGAC 4 cut(s) 594, 819, 1532, 2196
BstX2I RGATCY 7 cut(s) 1172, 2065, 2125, 2395, 2403, 3562, 3774
BstXI CCANNNNNNTGG 5 cut(s) 2298, 2572, 3221, 4084, 4286
BstYI RGATCY 7 cut(s) 1172, 2065, 2125, 2395, 2403, 3562, 3774
Bsu15I ATCGAT 1 cut(s) 4257
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 8 cut(s) 390, 730, 847, 1066, 2564, 2829, 2993, 3167
BsuTUI ATCGAT 1 cut(s) 4257
BtgZI GCGATG 1 cut(s) 4400
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 9 cut(s) 245, 275, 962, 1337, 2310, 2954, 3405, 3545, 4638
BveI ACCTGC 3 cut(s) 1369, 1522, 3584
Cac8I GCNNGC 7 cut(s) 388, 714, 1017, 1504, 2659, 3934, 4460
CaiI CAGNNNCTG 2 cut(s) 425, 3708
CciI TCATGA 2 cut(s) 238, 349
CfoI GCGC 2 cut(s) 3232, 3240
Cfr13I GGNCC 9 cut(s) 367, 397, 729, 2827, 3165, 3474, 3916, 4246, 4357
ClaI ATCGAT 1 cut(s) 4257
Csp6I GTAC 9 cut(s) 176, 834, 2429, 2804, 2937, 3184, 3725, 3868, 4699
CviQI GTAC 9 cut(s) 176, 834, 2429, 2804, 2937, 3184, 3725, 3868, 4699
DraI TTTAAA 4 cut(s) 468, 1096, 1812, 3000
EaeI YGGCCR 1 cut(s) 2562
Eam1104I CTCTTC 7 cut(s) 2739, 2814, 3252, 4023, 4029, 4163, 4437
EarI CTCTTC 7 cut(s) 2739, 2814, 3252, 4023, 4029, 4163, 4437
EciI GGCGGA 1 cut(s) 584
Eco130I CCWWGG 2 cut(s) 2683, 3579
Eco24I GRGCYC 3 cut(s) 948, 2343, 3722
Eco47I GGWCC 6 cut(s) 367, 397, 3474, 3916, 4246, 4357
Eco57I CTGAAG 7 cut(s) 281, 2334, 2874, 3609, 3848, 4004, 4089
EcoNI CCTNNNNNAGG 1 cut(s) 736
EcoO109I RGGNCCY 4 cut(s) 367, 397, 2827, 3916
EcoRII CCWGG 5 cut(s) 390, 582, 1456, 2421, 2864
EcoT14I CCWWGG 2 cut(s) 2683, 3579
EcoT22I ATGCAT 3 cut(s) 225, 2552, 2583
EcoT38I GRGCYC 3 cut(s) 948, 2343, 3722
ErhI CCWWGG 2 cut(s) 2683, 3579
Esp3I CGTCTC 1 cut(s) 1854
FalI AAGNNNNNCTT 2 cut(s) 4647, 4679
FaqI GGGAC 5 cut(s) 2155, 2714, 3460, 3902, 4370
FauI CCCGC 1 cut(s) 4347
FauNDI CATATG 3 cut(s) 2710, 3289, 4164
FbaI TGATCA 5 cut(s) 157, 2119, 2409, 2758, 3366
FblI GTMKAC 2 cut(s) 274, 1643
FriOI GRGCYC 3 cut(s) 948, 2343, 3722
FspI TGCGCA 1 cut(s) 3231
GlaI GCGC 2 cut(s) 3231, 3239
GsaI CCCAGC 2 cut(s) 3421, 4295
GsuI CTGGAG 6 cut(s) 156, 413, 1467, 2848, 2912, 4694
HaeIII GGCC 8 cut(s) 390, 730, 847, 1066, 2564, 2829, 2993, 3167
HapII CCGG 3 cut(s) 364, 3498, 3956
HhaI GCGC 2 cut(s) 3232, 3240
Hin6I GCGC 2 cut(s) 3230, 3238
HinP1I GCGC 2 cut(s) 3230, 3238
HincII GTYRAC 1 cut(s) 1762
HindII GTYRAC 1 cut(s) 1762
HindIII AAGCTT 8 cut(s) 2285, 2930, 3011, 3326, 3837, 4233, 4472, 4736
HpaII CCGG 3 cut(s) 364, 3498, 3956
HphI GGTGA 8 cut(s) 485, 875, 980, 2470, 2545, 3673, 3822, 3881
Hpy166II GTNNAC 6 cut(s) 275, 1644, 1678, 1762, 3599, 4701
Hpy8I GTNNAC 6 cut(s) 275, 1644, 1678, 1762, 3599, 4701
Hpy99I CGWCG 1 cut(s) 3653
HpyCH4III ACNGT 7 cut(s) 137, 1029, 1843, 3747, 3973, 4698, 4745
HspAI GCGC 2 cut(s) 3230, 3238
Ksp22I TGATCA 5 cut(s) 157, 2119, 2409, 2758, 3366
LguI GCTCTTC 1 cut(s) 4023
LmnI GCTCC 8 cut(s) 175, 951, 2446, 2591, 2698, 2719, 2893, 4333
MbiI CCGCTC 1 cut(s) 2196
MfeI CAATTG 3 cut(s) 3407, 3728, 3927
MflI RGATCY 7 cut(s) 1172, 2065, 2125, 2395, 2403, 3562, 3774
MhlI GDGCHC 8 cut(s) 193, 948, 2343, 3575, 3601, 3722, 4381, 4640
MlsI TGGCCA 1 cut(s) 2564
MluNI TGGCCA 1 cut(s) 2564
MlyI GAGTC 8 cut(s) 823, 833, 1096, 1145, 1781, 2776, 3809, 4107
MmeI TCCRAC 5 cut(s) 762, 1137, 1470, 2824, 3136
Mox20I TGGCCA 1 cut(s) 2564
Mph1103I ATGCAT 3 cut(s) 225, 2552, 2583
MroXI GAANNNNTTC 2 cut(s) 90, 1738
MscI TGGCCA 1 cut(s) 2564
Msp20I TGGCCA 1 cut(s) 2564
MspA1I CMGCKG 3 cut(s) 452, 1375, 1970
MspCI CTTAAG 1 cut(s) 2321
MspI CCGG 3 cut(s) 364, 3498, 3956
MspR9I CCNGG 8 cut(s) 365, 392, 584, 1458, 2423, 2866, 3498, 3957
MunI CAATTG 3 cut(s) 3407, 3728, 3927
Mva1269I GAATGC 3 cut(s) 64, 484, 3379
MvaI CCWGG 5 cut(s) 392, 584, 1458, 2423, 2866
MvnI CGCG 1 cut(s) 721
NciI CCSGG 3 cut(s) 365, 3498, 3957
NdeI CATATG 3 cut(s) 2710, 3289, 4164
NmeAIII GCCGAG 1 cut(s) 1576
NmuCI GTSAC 8 cut(s) 126, 968, 2779, 2950, 3679, 3710, 3756, 3887
NsbI TGCGCA 1 cut(s) 3231
NsiI ATGCAT 3 cut(s) 225, 2552, 2583
NspI RCATGY 9 cut(s) 223, 381, 1506, 1838, 2061, 2585, 3365, 3764, 3936
NspV TTCGAA 1 cut(s) 1166
OliI CACNNNNGTG 1 cut(s) 2315
PacI TTAATTAA 1 cut(s) 2037
PaeI GCATGC 2 cut(s) 1506, 3936
PagI TCATGA 2 cut(s) 238, 349
PaqCI CACCTGC 2 cut(s) 1369, 3584
PciI ACATGT 2 cut(s) 3361, 3760
PciSI GCTCTTC 1 cut(s) 4023
PctI GAATGC 3 cut(s) 64, 484, 3379
PdmI GAANNNNTTC 2 cut(s) 90, 1738
PflFI GACNNNGTC 1 cut(s) 365
PflMI CCANNNNNTGG 2 cut(s) 2689, 2843
PfoI TCCNGGA 1 cut(s) 2864
PleI GAGTC 8 cut(s) 822, 832, 1095, 1144, 1780, 2776, 3808, 4106
PpsI GAGTC 8 cut(s) 822, 832, 1095, 1144, 1780, 2776, 3808, 4106
Ppu21I YACGTR 3 cut(s) 163, 179, 2009
PpuMI RGGWCCY 3 cut(s) 367, 397, 3916
PscI ACATGT 2 cut(s) 3361, 3760
PshBI ATTAAT 4 cut(s) 677, 1689, 4497, 4631
Psp1406I AACGTT 3 cut(s) 925, 3004, 4216
Psp5II RGGWCCY 3 cut(s) 367, 397, 3916
Psp6I CCWGG 5 cut(s) 390, 582, 1456, 2421, 2864
PspFI CCCAGC 2 cut(s) 3417, 4291
PspGI CCWGG 5 cut(s) 390, 582, 1456, 2421, 2864
PspPI GGNCC 9 cut(s) 367, 397, 729, 2827, 3165, 3474, 3916, 4246, 4357
PspPPI RGGWCCY 3 cut(s) 367, 397, 3916
PstI CTGCAG 6 cut(s) 430, 1276, 2092, 2356, 4315, 4564
PstNI CAGNNNCTG 2 cut(s) 425, 3708
PsuI RGATCY 7 cut(s) 1172, 2065, 2125, 2395, 2403, 3562, 3774
PsyI GACNNNGTC 1 cut(s) 365
PvuII CAGCTG 2 cut(s) 452, 1970
RsaI GTAC 9 cut(s) 177, 835, 2430, 2805, 2938, 3185, 3726, 3869, 4700
RsaNI GTAC 9 cut(s) 176, 834, 2429, 2804, 2937, 3184, 3725, 3868, 4699
SapI GCTCTTC 1 cut(s) 4023
Sau96I GGNCC 9 cut(s) 367, 397, 729, 2827, 3165, 3474, 3916, 4246, 4357
ScaI AGTACT 2 cut(s) 835, 3869
SchI GAGTC 8 cut(s) 823, 833, 1096, 1145, 1781, 2776, 3809, 4107
ScrFI CCNGG 8 cut(s) 365, 392, 584, 1458, 2423, 2866, 3498, 3957
SduI GDGCHC 8 cut(s) 193, 948, 2343, 3575, 3601, 3722, 4381, 4640
SfcI CTRYAG 7 cut(s) 426, 489, 1272, 2088, 2352, 4311, 4560
SfuI TTCGAA 1 cut(s) 1166
SinI GGWCC 6 cut(s) 367, 397, 3474, 3916, 4246, 4357
SmlI CTYRAG 5 cut(s) 192, 2082, 2242, 2321, 3778
SmoI CTYRAG 5 cut(s) 192, 2082, 2242, 2321, 3778
SpeI ACTAGT 1 cut(s) 4009
SphI GCATGC 2 cut(s) 1506, 3936
SsiI CCGC 9 cut(s) 334, 569, 719, 775, 1232, 1375, 2194, 4186, 4354
StyD4I CCNGG 8 cut(s) 363, 390, 582, 1456, 2421, 2864, 3496, 3955
StyI CCWWGG 2 cut(s) 2683, 3579
TaaI ACNGT 7 cut(s) 137, 1029, 1843, 3747, 3973, 4698, 4745
TaqI TCGA 9 cut(s) 415, 830, 1166, 1700, 3250, 3383, 3651, 4257, 4440
TatI WGTACW 6 cut(s) 833, 2936, 3183, 3724, 3867, 4698
TauI GCSGC 3 cut(s) 337, 1378, 4189
TscAI CASTG 9 cut(s) 252, 275, 962, 1344, 2317, 2961, 3405, 3552, 4645
TseFI GTSAC 8 cut(s) 126, 968, 2779, 2950, 3679, 3710, 3756, 3887
Tsp45I GTSAC 8 cut(s) 126, 968, 2779, 2950, 3679, 3710, 3756, 3887
TspGWI ACGGA 1 cut(s) 3637
TspRI CASTG 9 cut(s) 252, 275, 962, 1344, 2317, 2961, 3405, 3552, 4645
Tth111I GACNNNGTC 1 cut(s) 365
Van91I CCANNNNNTGG 2 cut(s) 2689, 2843
Vha464I CTTAAG 1 cut(s) 2321
VneI GTGCAC 1 cut(s) 3597
VpaK11BI GGWCC 6 cut(s) 367, 397, 3474, 3916, 4246, 4357
VspI ATTAAT 4 cut(s) 677, 1689, 4497, 4631
XagI CCTNNNNNAGG 1 cut(s) 736
XapI RAATTY 5 cut(s) 86, 288, 889, 915, 4610
XbaI TCTAGA 3 cut(s) 1298, 2380, 2543
XceI RCATGY 9 cut(s) 223, 381, 1506, 1838, 2061, 2585, 3365, 3764, 3936
XcmI CCANNNNNNNNNTGG 2 cut(s) 1657, 3533
XmiI GTMKAC 2 cut(s) 274, 1643
XmnI GAANNNNTTC 2 cut(s) 90, 1738
ZrmI AGTACT 2 cut(s) 835, 3869
Zsp2I ATGCAT 3 cut(s) 225, 2552, 2583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.