pycom03g00180

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013050
Physical Location & Seq
Reverse (-)
170458 .. 174391
3934 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGGTGAAGACAGACGTACAGAAAGTACATATGAAGAAAAAATTGGGATTTTGGAGGGTTCTCACTCATCTCAGGATACAGCAGAGAACCATGATGACTTATCTGCTAAGGATGATCGATAGTGATACTCCTGAACATAAAGGATTGTTGGTTGAGAGTGTAGAACAGCATGCAGAAAAGCAGAAAGAAGAGCCTGTTGCTATATCTGAAACTGACTTTGATTCTGGAAGCAAGGTGCCTCCTGATGAGGGCAAGGATGAATCTGAAAAGACTGAGTCATTGTCCAGGGAAGAGTTAGGCCGCCGTGTTGCTTCTCGTTGGACTGGGGAAGATACCTCAGAGGAAGGAGGGGAAGTTGATGTTGAAAAAGATAATAATCACGAAGAAGTTCCAAAGGACACCCATGATGAGGATGATGGCTATGCTTCAGAAACTGATGATGAGTCCCAAAGATACGATGATGAAGATGTAGAGGAGGAGGCAGATAATGTTTTTGGAGAGGAGGATCACGGGGATTCTAGTTCTTACAAATACGAGTCAGACACTGATTCAGACTTTTCAGATGTAACAACCTCAAGCAAACCATCTTGGTGGGAGAAGATACAAAAAACTGTGCGGGACCTTTTACAGGCTGTTAATTTATTCCAAACTCCAGTGAACCAATCAGAGGCTGCTAGTATACGCAAAGAGTATGATGAATCCAGTGCCAAATATGTTTACAAAGTATGCCCATATAAACAAGCTTCCCAGGTGGAGGGACACTCTACAACCCGTTTAGGGAGCTGGGACAAATTTGAGGACTCGTATAAAATCATGGTCTTTGCAAATGGTGATAAGTGCTGGAATGGGCCTGATAGAAGTTTGAAGGTCAGACTTAAATGTGGTTTGAAAAATGAGGTTGCTGATGTTGATGAACCAAGTCGTTGCGAGTATGTAGCGTTGTTATCTACACCAGCGCTTTGCTCAGAGGAAAAACTTGAGGAACTGCAACACAAATTAGACGTATTGAACAAAGAAGAGCCGCAAGGCCACGATGAACTTTAA

Protein Analysis

348

Amino Acids

39.72

Weight (kDa)

4.59

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH_1 PF13015 231 - 330 4.9e-21 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 679
AciI CCGC 3 cut(s) 301, 616, 1022
AclWI GGATC 1 cut(s) 513
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 2 cut(s) 18, 27
AfeI AGCGCT 1 cut(s) 957
AfiI CCNNNNNNNGG 6 cut(s) 248, 409, 628, 667, 754, 777
AgsI TTSAA 4 cut(s) 365, 865, 889, 1009
AjnI CCWGG 2 cut(s) 284, 747
AjuI GAANNNNNNNTTGG 2 cut(s) 26, 58
AluBI AGCT 2 cut(s) 743, 783
AluI AGCT 2 cut(s) 743, 783
AlwI GGATC 1 cut(s) 513
AlwNI CAGNNNCTG 3 cut(s) 434, 545, 671
Aor51HI AGCGCT 1 cut(s) 957
AoxI GGCC 3 cut(s) 298, 848, 1027
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 1 cut(s) 958
AspS9I GGNCC 2 cut(s) 619, 848
AsuHPI GGTGA 2 cut(s) 16, 842
AvaII GGWCC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 658
BccI CCATC 2 cut(s) 410, 592
BceAI ACGGC 1 cut(s) 288
BciT130I CCWGG 2 cut(s) 286, 749
BciVI GTATCC 1 cut(s) 69
BfaI CTAG 2 cut(s) 519, 675
BfoI RGCGCY 1 cut(s) 959
BfuI GTATCC 1 cut(s) 69
BisI GCNGC 3 cut(s) 301, 672, 1022
BlsI GCNGC 3 cut(s) 302, 673, 1023
Bme1390I CCNGG 2 cut(s) 286, 749
Bme18I GGWCC 1 cut(s) 619
BmgT120I GGNCC 2 cut(s) 619, 848
BmiI GGNNCC 2 cut(s) 237, 620
BmrFI CCNGG 2 cut(s) 286, 749
BmrI ACTGGG 1 cut(s) 333
BmuI ACTGGG 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 746, 778
BpmI CTGGAG 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 559, 998
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 285, 747
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 6 cut(s) 248, 409, 628, 667, 754, 777
Bse1I ACTGG 3 cut(s) 328, 653, 702
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 2 cut(s) 286, 749
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 285, 747
BseGI GGATG 3 cut(s) 117, 262, 418
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 6 cut(s) 248, 409, 628, 667, 754, 777
BseMII CTCAG 4 cut(s) 85, 264, 351, 978
BseNI ACTGG 3 cut(s) 328, 653, 702
BseRI GAGGAG 3 cut(s) 488, 491, 515
BseXI GCAGC 1 cut(s) 658
BseYI CCCAGC 1 cut(s) 783
BshFI GGCC 3 cut(s) 300, 850, 1029
BshNI GGYRCC 1 cut(s) 235
BshVI ATCGAT 1 cut(s) 117
BslFI GGGAC 4 cut(s) 430, 632, 771, 800
BslI CCNNNNNNNGG 6 cut(s) 248, 409, 628, 667, 754, 777
BsmFI GGGAC 4 cut(s) 430, 632, 771, 800
BsnI GGCC 3 cut(s) 300, 850, 1029
Bsp143I GATC 2 cut(s) 114, 505
BspACI CCGC 3 cut(s) 301, 616, 1022
BspANI GGCC 3 cut(s) 300, 850, 1029
BspCNI CTCAG 4 cut(s) 84, 265, 350, 977
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 237, 620
BspPI GGATC 1 cut(s) 513
BspQI GCTCTTC 2 cut(s) 183, 1011
BspT107I GGYRCC 1 cut(s) 235
BsrI ACTGG 3 cut(s) 328, 653, 702
BssECI CCNNGG 2 cut(s) 285, 747
BssMI GATC 2 cut(s) 114, 505
BssNAI GTATAC 1 cut(s) 680
Bst1107I GTATAC 1 cut(s) 680
Bst2UI CCWGG 2 cut(s) 286, 749
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 3 cut(s) 183, 285, 1011
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 5 cut(s) 71, 107, 273, 337, 964
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 3 cut(s) 117, 262, 418
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 1 cut(s) 958
BstKTI GATC 2 cut(s) 117, 508
BstMBI GATC 2 cut(s) 114, 505
BstNI CCWGG 2 cut(s) 286, 749
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 2 cut(s) 284, 747
BstV1I GCAGC 1 cut(s) 658
BstV2I GAAGAC 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 591
BstZ17I GTATAC 1 cut(s) 680
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 3 cut(s) 300, 850, 1029
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 3 cut(s) 117, 262, 418
BtsIMutI CAGTG 3 cut(s) 543, 660, 709
Cac8I GCNNGC 1 cut(s) 171
CaiI CAGNNNCTG 3 cut(s) 434, 545, 671
CfoI GCGC 1 cut(s) 958
Cfr13I GGNCC 2 cut(s) 619, 848
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 2 cut(s) 17, 26
CviAII CATG 4 cut(s) 91, 170, 404, 814
CviQI GTAC 2 cut(s) 17, 26
DdeI CTNAG 5 cut(s) 71, 107, 273, 337, 964
DpnI GATC 2 cut(s) 116, 507
DpnII GATC 2 cut(s) 114, 505
Eam1104I CTCTTC 3 cut(s) 183, 285, 1011
EarI CTCTTC 3 cut(s) 183, 285, 1011
Eco47I GGWCC 1 cut(s) 619
Eco47III AGCGCT 1 cut(s) 957
Eco57I CTGAAG 1 cut(s) 411
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 284, 747
FaeI CATG 4 cut(s) 94, 173, 407, 817
FaqI GGGAC 4 cut(s) 430, 632, 771, 800
FatI CATG 4 cut(s) 90, 169, 403, 813
FauI CCCGC 1 cut(s) 609
FauNDI CATATG 1 cut(s) 30
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 301, 672, 1022
FokI GGATG 3 cut(s) 124, 269, 425
Fsp4HI GCNGC 3 cut(s) 301, 672, 1022
FspBI CTAG 2 cut(s) 519, 675
GlaI GCGC 1 cut(s) 957
GluI GCNGC 3 cut(s) 301, 672, 1022
GsaI CCCAGC 1 cut(s) 787
GsuI CTGGAG 1 cut(s) 636
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 3 cut(s) 300, 850, 1029
HhaI GCGC 1 cut(s) 958
Hin1II CATG 4 cut(s) 94, 173, 407, 817
Hin6I GCGC 1 cut(s) 956
HinP1I GCGC 1 cut(s) 956
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 9 cut(s) 221, 260, 275, 443, 515, 536, 548, 698, 800
HphI GGTGA 2 cut(s) 16, 842
Hpy166II GTNNAC 3 cut(s) 658, 680, 718
Hpy188III TCNNGA 5 cut(s) 73, 131, 225, 242, 380
Hpy8I GTNNAC 3 cut(s) 658, 680, 718
HpyAV CCTTC 2 cut(s) 338, 859
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 2 cut(s) 15, 1002
HpyCH4V TGCA 3 cut(s) 173, 824, 988
HpyF3I CTNAG 5 cut(s) 71, 107, 273, 337, 964
HpySE526I ACGT 2 cut(s) 15, 1002
Hsp92II CATG 4 cut(s) 94, 173, 407, 817
HspAI GCGC 1 cut(s) 956
Kzo9I GATC 2 cut(s) 114, 505
LguI GCTCTTC 2 cut(s) 183, 1011
LmnI GCTCC 1 cut(s) 780
Lsp1109I GCAGC 1 cut(s) 658
MaeI CTAG 2 cut(s) 519, 675
MaeII ACGT 2 cut(s) 15, 1002
MaeIII GTNAC 1 cut(s) 565
MalI GATC 2 cut(s) 116, 507
MboI GATC 2 cut(s) 114, 505
MboII GAAGA 9 cut(s) 19, 46, 200, 302, 341, 395, 476, 610, 1028
MluCI AATT 4 cut(s) 41, 637, 791, 995
MlyI GAGTC 4 cut(s) 284, 452, 545, 794
MmeI TCCRAC 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 387
MseI TTAA 3 cut(s) 636, 876, 1042
MslI CAYNNNNRTG 1 cut(s) 589
MspR9I CCNGG 2 cut(s) 286, 749
MvaI CCWGG 2 cut(s) 286, 749
NdeI CATATG 1 cut(s) 30
NdeII GATC 2 cut(s) 114, 505
NlaIII CATG 4 cut(s) 94, 173, 407, 817
NlaIV GGNNCC 2 cut(s) 237, 620
NspI RCATGY 1 cut(s) 173
PaeI GCATGC 1 cut(s) 173
PciSI GCTCTTC 2 cut(s) 183, 1011
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 5 cut(s) 221, 260, 515, 548, 698
PflFI GACNNNGTC 1 cut(s) 274
PkrI GCNGC 3 cut(s) 302, 673, 1023
PleI GAGTC 4 cut(s) 283, 451, 544, 794
PpsI GAGTC 4 cut(s) 283, 451, 544, 794
PpuMI RGGWCCY 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 2 cut(s) 284, 747
PspFI CCCAGC 1 cut(s) 783
PspGI CCWGG 2 cut(s) 284, 747
PspN4I GGNNCC 2 cut(s) 237, 620
PspPI GGNCC 2 cut(s) 619, 848
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 3 cut(s) 434, 545, 671
PsyI GACNNNGTC 1 cut(s) 274
RsaI GTAC 2 cut(s) 18, 27
RsaNI GTAC 2 cut(s) 17, 26
RseI CAYNNNNRTG 1 cut(s) 589
SapI GCTCTTC 2 cut(s) 183, 1011
SaqAI TTAA 3 cut(s) 636, 876, 1042
SatI GCNGC 3 cut(s) 301, 672, 1022
Sau3AI GATC 2 cut(s) 114, 505
Sau96I GGNCC 2 cut(s) 619, 848
SchI GAGTC 4 cut(s) 284, 452, 545, 794
ScrFI CCNGG 2 cut(s) 286, 749
SinI GGWCC 1 cut(s) 619
SmiMI CAYNNNNRTG 1 cut(s) 589
SmlI CTYRAG 2 cut(s) 574, 977
SmoI CTYRAG 2 cut(s) 574, 977
SphI GCATGC 1 cut(s) 173
Sse9I AATT 4 cut(s) 41, 637, 791, 995
SsiI CCGC 3 cut(s) 301, 616, 1022
SspMI CTAG 2 cut(s) 519, 675
StyD4I CCNGG 2 cut(s) 284, 747
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 2 cut(s) 18, 1005
TaqI TCGA 1 cut(s) 117
TasI AATT 4 cut(s) 41, 637, 791, 995
TatI WGTACW 1 cut(s) 25
TauI GCSGC 2 cut(s) 303, 1024
TfiI GAWTC 5 cut(s) 221, 260, 515, 548, 698
Tru1I TTAA 3 cut(s) 636, 876, 1042
Tru9I TTAA 3 cut(s) 636, 876, 1042
TscAI CASTG 3 cut(s) 550, 660, 709
TseI GCWGC 1 cut(s) 671
TspDTI ATGAA 5 cut(s) 47, 273, 477, 711, 927
TspRI CASTG 3 cut(s) 550, 660, 709
Tth111I GACNNNGTC 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 619
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 1 cut(s) 791
XceI RCATGY 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 2 cut(s) 519, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.