pycom03g00460

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013741
Physical Location & Seq
Forward (+)
381009 .. 381697
689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 399 bp
ATGTACAGGGGTGATGTAGTTCCCAAGGATGTCAATGCTGCCGTTGCAACTATCAAAACAAAAAGGACTGTGCAGTTTGTTGACTGGTGCCCAACAGGTTTCAAATGTGGTATTAACTATCAGCCTCCAACAGTTGTACCAGGGGGTGATCTTGCCAAGGTTCAGCGAGCAGTGTGCATGATCAGTAACAACACGGCAGTGGCTGAGGTGTTCTCACGCATTGACCACAAATTTGACCTCATGTATTCCAAGAGGGCATTCGTTCACTGGTATGTTGGTGAGGGAATGGAAGAAGGAGAGTTTTCAGAAGCTCGTGAAGATCTGGCTGCTCTAGAGAAAGATTACGAGGAAGTTGGCGCCGAAGGTGTTGATGATGAAGAGGGAAGTGAAGGTTACTAG

Protein Analysis

133

Amino Acids

14.68

Weight (kDa)

4.6

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 1 - 75 3e-25 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 87, 356
AcsI RAATTY 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 2 cut(s) 5, 138
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
AlwNI CAGNNNCTG 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 38, 326
ApoI RAATTY 1 cut(s) 230
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspLEI GCGC 1 cut(s) 359
AsuHPI GGTGA 3 cut(s) 23, 158, 290
BaeGI GKGCMC 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BanI GGYRCC 2 cut(s) 87, 356
BauI CACGAG 1 cut(s) 312
BbvCI CCTCAGC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 25, 313
BceAI ACGGC 2 cut(s) 26, 210
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 1 cut(s) 141
BclI TGATCA 1 cut(s) 180
BfaI CTAG 2 cut(s) 332, 397
BfoI RGCGCY 1 cut(s) 360
BglII AGATCT 1 cut(s) 319
BisI GCNGC 2 cut(s) 39, 327
BlsI GCNGC 2 cut(s) 40, 328
Bme1390I CCNGG 1 cut(s) 141
BmiI GGNNCC 2 cut(s) 89, 358
BmrFI CCNGG 1 cut(s) 141
BplI GAGNNNNNCTC 2 cut(s) 197, 229
Bpu10I CCTNAGC 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 3 cut(s) 24, 140, 156
Bse1I ACTGG 2 cut(s) 89, 272
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 24, 140, 156
BseGI GGATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 89, 272
BseSI GKGCMC 1 cut(s) 92
BseXI GCAGC 2 cut(s) 25, 313
BsgI GTGCAG 1 cut(s) 92
BshNI GGYRCC 2 cut(s) 87, 356
BsmI GAATGC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 92
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 148, 180, 319
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 89, 358
BspT107I GGYRCC 2 cut(s) 87, 356
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 89, 272
BssECI CCNNGG 3 cut(s) 24, 140, 156
BssMI GATC 3 cut(s) 148, 180, 319
BssNI GRCGYC 1 cut(s) 357
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 24, 156
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 70, 133
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 357
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 34
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 3 cut(s) 151, 183, 322
BstMBI GATC 3 cut(s) 148, 180, 319
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 25, 313
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 2 cut(s) 177, 204
BtsIMutI CAGTG 3 cut(s) 177, 204, 265
Cac8I GCNNGC 1 cut(s) 168
CaiI CAGNNNCTG 1 cut(s) 203
CfoI GCGC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 4, 137
CviAII CATG 2 cut(s) 178, 241
CviJI RGCY 4 cut(s) 124, 203, 311, 326
CviKI_1 RGCY 4 cut(s) 124, 203, 311, 326
CviQI GTAC 2 cut(s) 4, 137
DdeI CTNAG 1 cut(s) 204
DinI GGCGCC 1 cut(s) 358
DpnI GATC 3 cut(s) 150, 182, 321
DpnII GATC 3 cut(s) 148, 180, 319
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 2 cut(s) 24, 156
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 2 cut(s) 24, 156
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 24, 156
FaeI CATG 2 cut(s) 181, 244
FaiI YATR 3 cut(s) 179, 242, 273
FatI CATG 2 cut(s) 177, 240
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 39, 327
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 2 cut(s) 39, 327
FspBI CTAG 2 cut(s) 332, 397
GlaI GCGC 1 cut(s) 358
GluI GCNGC 2 cut(s) 39, 327
HaeII RGCGCY 1 cut(s) 360
HhaI GCGC 1 cut(s) 359
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 2 cut(s) 181, 244
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HphI GGTGA 3 cut(s) 23, 158, 290
Hpy166II GTNNAC 2 cut(s) 82, 265
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 2 cut(s) 314, 332
Hpy8I GTNNAC 2 cut(s) 82, 265
HpyAV CCTTC 3 cut(s) 287, 356, 383
HpyCH4III ACNGT 2 cut(s) 70, 133
HpyCH4V TGCA 3 cut(s) 47, 73, 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 2 cut(s) 181, 244
HspAI GCGC 1 cut(s) 357
KasI GGCGCC 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 3 cut(s) 148, 180, 319
LpnPI CCDG 6 cut(s) 70, 81, 126, 153, 253, 308
Lsp1109I GCAGC 2 cut(s) 25, 313
MaeI CTAG 2 cut(s) 332, 397
MaeIII GTNAC 2 cut(s) 185, 392
MalI GATC 3 cut(s) 150, 182, 321
MboI GATC 3 cut(s) 148, 180, 319
MboII GAAGA 3 cut(s) 302, 329, 389
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 7 cut(s) 135, 199, 246, 248, 274, 340, 373
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 2 cut(s) 197, 270
MspR9I CCNGG 1 cut(s) 141
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 1 cut(s) 44
NarI GGCGCC 1 cut(s) 357
NdeII GATC 3 cut(s) 148, 180, 319
NlaIII CATG 2 cut(s) 181, 244
NlaIV GGNNCC 2 cut(s) 89, 358
OliI CACNNNNGTG 1 cut(s) 197
PctI GAATGC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 40, 328
PluTI GGCGCC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 89, 358
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 319
RsaI GTAC 2 cut(s) 5, 138
RsaNI GTAC 2 cut(s) 4, 137
RseI CAYNNNNRTG 2 cut(s) 197, 270
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 2 cut(s) 39, 327
Sau3AI GATC 3 cut(s) 148, 180, 319
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 92
SetI ASST 7 cut(s) 100, 162, 210, 240, 313, 367, 394
SfoI GGCGCC 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 197, 270
Sse9I AATT 1 cut(s) 230
SspDI GGCGCC 1 cut(s) 356
SspMI CTAG 2 cut(s) 332, 397
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 2 cut(s) 24, 156
TaaI ACNGT 2 cut(s) 70, 133
TasI AATT 1 cut(s) 230
TatI WGTACW 1 cut(s) 3
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 3 cut(s) 177, 204, 272
TseI GCWGC 2 cut(s) 38, 326
TspDTI ATGAA 1 cut(s) 390
TspRI CASTG 3 cut(s) 177, 204, 272
XapI RAATTY 1 cut(s) 230
XbaI TCTAGA 1 cut(s) 331
XspI CTAG 2 cut(s) 332, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.