Rroxscaffold_2G00082710

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013741
Physical Location & Seq
Forward (+)
5485122 .. 5485673
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082710.1

Sequence Viewer

Length: 552 bp
ATGCTTTCATCCTATGCACCAGTGATCTCTGCTGAGAAGGCCTACCATGAGCAACTCTCTGTGGCTGAAATCACCAACAGCGCCTTTGAGCCGGCCTCTATGATGGCCAAATGTGATCCTAGGCATGGGAAGTATATGGCTTGCTGCCTGATGTACAGAGGAGATGTGGTACCGAAGGATGTGAATGCTGCTGTGGCCACAATCAAGACCAAGAGGACCATTCAGTTTGTGGATTGGTGCCCAACTGGGTTCAAGTGTGGTATCAACTACCAGCCCCCTACTGTTGTACCTGGTGGTGACTTGGCCAAGGTTCAGAGGGCTGTGTGCATGATTTCCAACTCCACAAGTGTTGCTGAAGTGTTTTCGAGGATTGATCACAAGTTTGATCTGATGTATGCTAAGCGTGCTTTTGTGCACTGGTATGTTGGTGAGGGTATGGAGGAGGGTGAGTTTTCAGAAGCTAGGGAGGACTTGGCTGCCCTTGAGAAGGATTATGAAGAAGTTGGGTTGGAGTCAGCTGAAGGTGAAGATGGTGAGGATGAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

183

Amino Acids

20.35

Weight (kDa)

4.7

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 2 - 125 4.4e-48 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 2 cut(s) 169, 237
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 3 cut(s) 105, 195, 303
AcuI CTGAAG 2 cut(s) 375, 540
AfaI GTAC 4 cut(s) 155, 171, 288, 548
AfiI CCNNNNNNNGG 2 cut(s) 125, 487
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 2 cut(s) 461, 518
AluI AGCT 2 cut(s) 461, 518
Alw21I GWGCWC 1 cut(s) 417
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 1 cut(s) 110
AoxI GGCC 5 cut(s) 39, 93, 105, 195, 303
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 3 cut(s) 144, 188, 476
Asp718I GGTACC 1 cut(s) 169
AspA2I CCTAGG 1 cut(s) 119
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 216
AsuHPI GGTGA 6 cut(s) 64, 308, 440, 458, 536, 545
AvaII GGWCC 1 cut(s) 216
AvrII CCTAGG 1 cut(s) 119
BaeGI GKGCMC 2 cut(s) 242, 417
BaeI ACNNNNGTAYC 2 cut(s) 270, 303
BalI TGGCCA 3 cut(s) 107, 197, 305
BanI GGYRCC 2 cut(s) 169, 237
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 3 cut(s) 131, 175, 463
BccI CCATC 2 cut(s) 97, 524
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 1 cut(s) 373
BfaI CTAG 3 cut(s) 120, 462, 550
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 3 cut(s) 145, 189, 477
BlnI CCTAGG 1 cut(s) 119
BlpI GCTNAGC 1 cut(s) 399
BlsI GCNGC 3 cut(s) 146, 190, 478
BmcAI AGTACT 1 cut(s) 548
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 216
BmiI GGNNCC 2 cut(s) 171, 239
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 255
BmuI ACTGGG 1 cut(s) 255
BplI GAGNNNNNCTC 4 cut(s) 41, 73, 80, 112
Bpu1102I GCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 503
BsaJI CCNNGG 2 cut(s) 119, 306
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 434, 464
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 487
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 20, 250, 422
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 119, 306
BseGI GGATG 3 cut(s) 8, 184, 544
BseLI CCNNNNNNNGG 2 cut(s) 125, 487
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 3 cut(s) 20, 250, 422
BseRI GAGGAG 2 cut(s) 174, 455
BseSI GKGCMC 2 cut(s) 242, 417
BseXI GCAGC 3 cut(s) 131, 175, 463
BshFI GGCC 5 cut(s) 41, 95, 107, 197, 305
BshNI GGYRCC 2 cut(s) 169, 237
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 125, 487
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 5 cut(s) 41, 95, 107, 197, 305
Bsp1286I GDGCHC 2 cut(s) 242, 417
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 4 cut(s) 24, 115, 373, 385
Bsp1720I GCTNAGC 1 cut(s) 399
BspANI GGCC 5 cut(s) 41, 95, 107, 197, 305
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 171, 239
BspPI GGATC 1 cut(s) 110
BspT107I GGYRCC 2 cut(s) 169, 237
BsrFI RCCGGY 1 cut(s) 91
BsrGI TGTACA 1 cut(s) 153
BsrI ACTGG 3 cut(s) 20, 250, 422
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 119, 306
BssMI GATC 4 cut(s) 24, 115, 373, 385
BssT1I CCWWGG 2 cut(s) 119, 306
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 537
BstAUI TGTACA 1 cut(s) 153
BstC8I GCNNGC 3 cut(s) 93, 142, 405
BstDEI CTNAG 2 cut(s) 33, 399
BstENI CCTNNNNNAGG 1 cut(s) 485
BstF5I GGATG 3 cut(s) 8, 184, 544
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 4 cut(s) 27, 118, 376, 388
BstMBI GATC 4 cut(s) 24, 115, 373, 385
BstMWI GCNNNNNNNGC 3 cut(s) 38, 194, 404
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSLI GKGCMC 2 cut(s) 242, 417
BstV1I GCAGC 3 cut(s) 131, 175, 463
BsuRI GGCC 5 cut(s) 41, 95, 107, 197, 305
BtsCI GGATG 3 cut(s) 8, 184, 544
BtsIMutI CAGTG 2 cut(s) 27, 415
Cac8I GCNNGC 3 cut(s) 93, 142, 405
CfoI GCGC 1 cut(s) 83
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 216
CsiI ACCWGGT 1 cut(s) 289
Csp6I GTAC 4 cut(s) 154, 170, 287, 547
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 3 cut(s) 47, 125, 328
CviQI GTAC 4 cut(s) 154, 170, 287, 547
DdeI CTNAG 2 cut(s) 33, 399
DpnI GATC 4 cut(s) 26, 117, 375, 387
DpnII GATC 4 cut(s) 24, 115, 373, 385
EaeI YGGCCR 3 cut(s) 105, 195, 303
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 2 cut(s) 119, 306
Eco147I AGGCCT 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 216
Eco57I CTGAAG 2 cut(s) 375, 540
EcoNI CCTNNNNNAGG 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 119, 306
ErhI CCWWGG 2 cut(s) 119, 306
FaeI CATG 3 cut(s) 50, 128, 331
FatI CATG 3 cut(s) 46, 124, 327
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 3 cut(s) 145, 189, 477
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 3 cut(s) 145, 189, 477
FspBI CTAG 3 cut(s) 120, 462, 550
GlaI GCGC 1 cut(s) 82
GluI GCNGC 3 cut(s) 145, 189, 477
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 5 cut(s) 41, 95, 107, 197, 305
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 83
Hin1II CATG 3 cut(s) 50, 128, 331
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 1 cut(s) 512
HpaII CCGG 1 cut(s) 92
HphI GGTGA 6 cut(s) 64, 308, 440, 458, 536, 545
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 3 cut(s) 315, 390, 457
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 4 cut(s) 31, 169, 481, 515
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4V TGCA 3 cut(s) 17, 327, 415
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 194, 404
HpyF3I CTNAG 2 cut(s) 33, 399
Hsp92II CATG 3 cut(s) 50, 128, 331
HspAI GCGC 1 cut(s) 81
KpnI GGTACC 1 cut(s) 173
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 24, 115, 373, 385
LpnPI CCDG 8 cut(s) 33, 105, 161, 231, 276, 284, 303, 403
Lsp1109I GCAGC 3 cut(s) 131, 175, 463
MabI ACCWGGT 1 cut(s) 289
MaeI CTAG 3 cut(s) 120, 462, 550
MaeIII GTNAC 1 cut(s) 296
MalI GATC 4 cut(s) 26, 117, 375, 387
MboI GATC 4 cut(s) 24, 115, 373, 385
MboII GAAGA 2 cut(s) 509, 539
MhlI GDGCHC 2 cut(s) 242, 417
MlsI TGGCCA 3 cut(s) 107, 197, 305
MluNI TGGCCA 3 cut(s) 107, 197, 305
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 2 cut(s) 360, 489
Mox20I TGGCCA 3 cut(s) 107, 197, 305
MroNI GCCGGC 1 cut(s) 91
MscI TGGCCA 3 cut(s) 107, 197, 305
MslI CAYNNNNRTG 1 cut(s) 420
Msp20I TGGCCA 3 cut(s) 107, 197, 305
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 291
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 38, 194, 404
NaeI GCCGGC 1 cut(s) 93
NdeII GATC 4 cut(s) 24, 115, 373, 385
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 3 cut(s) 50, 128, 331
NlaIV GGNNCC 2 cut(s) 171, 239
NmuCI GTSAC 1 cut(s) 296
PceI AGGCCT 1 cut(s) 41
PctI GAATGC 1 cut(s) 190
PdiI GCCGGC 1 cut(s) 93
PkrI GCNGC 3 cut(s) 146, 190, 478
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 171, 239
PspPI GGNCC 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 4 cut(s) 155, 171, 288, 548
RsaNI GTAC 4 cut(s) 154, 170, 287, 547
RseI CAYNNNNRTG 1 cut(s) 420
SatI GCNGC 3 cut(s) 145, 189, 477
Sau3AI GATC 4 cut(s) 24, 115, 373, 385
Sau96I GGNCC 1 cut(s) 216
ScaI AGTACT 1 cut(s) 548
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 2 cut(s) 242, 417
SetI ASST 5 cut(s) 292, 312, 463, 520, 526
SexAI ACCWGGT 1 cut(s) 289
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SseBI AGGCCT 1 cut(s) 41
SspMI CTAG 3 cut(s) 120, 462, 550
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 119, 306
TaaI ACNGT 1 cut(s) 283
TaqI TCGA 1 cut(s) 365
TatI WGTACW 2 cut(s) 153, 546
TscAI CASTG 2 cut(s) 27, 422
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 3 cut(s) 144, 188, 476
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 2 cut(s) 27, 422
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmaJI CCTAGG 1 cut(s) 119
XspI CTAG 3 cut(s) 120, 462, 550
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.