Rroxscaffold_3G00228380

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013741
Physical Location & Seq
Forward (+)
12789809 .. 12792084
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228380.1

Sequence Viewer

Length: 555 bp
ATGCTCTCCTCCTATGCTCCTGTGATCTCCGCAGAGAAAGCCTACCATGAGCAGCTCTCAGTGGCTGAGATCACCAACAGTGCTTTTGAGCCAGCTTCCATGATGGCCAAGTGTGATCCTCGCCATGGCAAGTACATGGCCTGCTGTTTGATGTACCGAGGAGACGTGGTGCCTAAGGATGTGAATGCGGCTGTGGCCACCATCAAGACCAAGCGTACCATTCAGTTTGTGGACTGGTGCCCTACTGGGTTCAAGTGTGGTATCAACTACCAGCCACCCACTGTTGTTCCTGGCGGTGACCTTGCTAAGGTGCAGAGGGCTGTGTGCATGATCTCCAACTCCACTAGTGTGGCTGAGGTGTTCTCCCGCATTGATCACAAGTTTGATCTGATGTACGCCAAGCGTGCTTTTGTGCATTGGTATGTGGGTGAGGGCATGGAGGAAGGAGAGTTCTCTGAGGCCCGTGAGGATCTTGCTGCTCTGGAGAAGGATTATGAGGAGGTTGGTGCCGAGTCTGCTGAGGGAGATGATGGTGATGAAGGAGACGAGTACTGA

Protein Analysis

184

Amino Acids

20.33

Weight (kDa)

4.68

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin_C PF03953 2 - 125 4.5e-48 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 169, 237, 506
AciI CCGC 4 cut(s) 30, 188, 294, 367
AclWI GGATC 2 cut(s) 110, 477
AcoI YGGCCR 2 cut(s) 105, 195
AfaI GTAC 5 cut(s) 134, 155, 217, 395, 551
AfiI CCNNNNNNNGG 2 cut(s) 125, 307
AgsI TTSAA 1 cut(s) 253
AhlI ACTAGT 1 cut(s) 344
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 347
AloI GAACNNNNNNTCC 2 cut(s) 434, 466
AluBI AGCT 2 cut(s) 55, 95
AluI AGCT 2 cut(s) 55, 95
Alw26I GTCTC 2 cut(s) 156, 537
AlwI GGATC 2 cut(s) 110, 477
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 4 cut(s) 105, 138, 195, 459
ApeKI GCWGC 2 cut(s) 52, 476
AspS9I GGNCC 1 cut(s) 460
AsuHPI GGTGA 4 cut(s) 64, 308, 440, 545
AxyI CCTNAGG 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 242
BalI TGGCCA 2 cut(s) 107, 197
BanI GGYRCC 3 cut(s) 169, 237, 506
BbvCI CCTCAGC 2 cut(s) 354, 519
BbvI GCAGC 2 cut(s) 64, 463
BccI CCATC 3 cut(s) 97, 209, 524
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 156, 537
BcuI ACTAGT 1 cut(s) 344
BfaI CTAG 1 cut(s) 345
BisI GCNGC 3 cut(s) 53, 189, 477
BlsI GCNGC 3 cut(s) 54, 190, 478
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 1 cut(s) 291
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 3 cut(s) 171, 239, 508
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 255
BmuI ACTGGG 1 cut(s) 255
BplI GAGNNNNNCTC 4 cut(s) 41, 73, 347, 379
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 3 cut(s) 306, 354, 519
BsaJI CCNNGG 2 cut(s) 124, 157
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 307
Bse1I ACTGG 2 cut(s) 239, 250
Bse21I CCTNAGG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 124, 157
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 2 cut(s) 125, 307
BseMII CTCAG 5 cut(s) 57, 72, 345, 447, 510
BseNI ACTGG 2 cut(s) 239, 250
BseRI GAGGAG 2 cut(s) 174, 512
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 2 cut(s) 64, 463
BsgI GTGCAG 1 cut(s) 332
BshFI GGCC 4 cut(s) 107, 140, 197, 461
BshNI GGYRCC 3 cut(s) 169, 237, 506
BslI CCNNNNNNNGG 2 cut(s) 125, 307
BsmAI GTCTC 2 cut(s) 156, 537
BsmBI CGTCTC 2 cut(s) 156, 537
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 4 cut(s) 107, 140, 197, 461
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 4 cut(s) 30, 188, 294, 367
BspANI GGCC 4 cut(s) 107, 140, 197, 461
BspCNI CTCAG 5 cut(s) 58, 71, 346, 448, 511
BspLI GGNNCC 3 cut(s) 171, 239, 508
BspPI GGATC 2 cut(s) 110, 477
BspT107I GGYRCC 3 cut(s) 169, 237, 506
BsrI ACTGG 2 cut(s) 239, 250
BssECI CCNNGG 2 cut(s) 124, 157
BssMI GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 80, 283
BstC8I GCNNGC 3 cut(s) 93, 142, 405
BstDEI CTNAG 7 cut(s) 58, 66, 174, 306, 354, 456, 519
BstDSI CCRYGG 1 cut(s) 124
BstEII GGTNACC 1 cut(s) 296
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 7 cut(s) 27, 72, 118, 333, 376, 388, 472
BstMAI GTCTC 2 cut(s) 156, 537
BstMBI GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
BstMWI GCNNNNNNNGC 4 cut(s) 38, 194, 404, 515
BstNI CCWGG 1 cut(s) 291
BstPI GGTNACC 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 289
BstSLI GKGCMC 1 cut(s) 242
BstV1I GCAGC 2 cut(s) 64, 463
BstX2I RGATCY 1 cut(s) 469
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 4 cut(s) 107, 140, 197, 461
BtgI CCRYGG 1 cut(s) 124
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 3 cut(s) 66, 85, 279
Cac8I GCNNGC 3 cut(s) 93, 142, 405
CaiI CAGNNNCTG 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 460
Csp6I GTAC 5 cut(s) 133, 154, 216, 394, 550
CviAII CATG 6 cut(s) 47, 100, 125, 136, 328, 436
CviQI GTAC 5 cut(s) 133, 154, 216, 394, 550
DdeI CTNAG 7 cut(s) 58, 66, 174, 306, 354, 456, 519
DpnI GATC 7 cut(s) 26, 71, 117, 332, 375, 387, 471
DpnII GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
EaeI YGGCCR 2 cut(s) 105, 195
Eco130I CCWWGG 1 cut(s) 124
Eco81I CCTNAGG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 124
Esp3I CGTCTC 2 cut(s) 156, 537
FaeI CATG 6 cut(s) 50, 103, 128, 139, 331, 439
FaiI YATR 9 cut(s) 15, 48, 101, 126, 137, 329, 423, 437, 495
FatI CATG 6 cut(s) 46, 99, 124, 135, 327, 435
FauI CCCGC 1 cut(s) 374
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 3 cut(s) 53, 189, 477
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 3 cut(s) 53, 189, 477
FspBI CTAG 1 cut(s) 345
GluI GCNGC 3 cut(s) 53, 189, 477
GsuI CTGGAG 1 cut(s) 503
HaeIII GGCC 4 cut(s) 107, 140, 197, 461
Hin1II CATG 6 cut(s) 50, 103, 128, 139, 331, 439
HinfI GANTC 1 cut(s) 512
HphI GGTGA 4 cut(s) 64, 308, 440, 545
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 2 cut(s) 390, 457
Hpy188III TCNNGA 2 cut(s) 205, 482
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 437, 481, 533
HpyCH4III ACNGT 2 cut(s) 80, 283
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 313, 327, 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 194, 404, 515
HpyF3I CTNAG 7 cut(s) 58, 66, 174, 306, 354, 456, 519
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 6 cut(s) 50, 103, 128, 139, 331, 439
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 9 cut(s) 33, 105, 154, 220, 231, 276, 284, 303, 467
Lsp1109I GCAGC 2 cut(s) 64, 463
MaeI CTAG 1 cut(s) 345
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 296
MalI GATC 7 cut(s) 26, 71, 117, 332, 375, 387, 471
MboI GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 242
MlsI TGGCCA 2 cut(s) 107, 197
MluNI TGGCCA 2 cut(s) 107, 197
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 360
Mox20I TGGCCA 2 cut(s) 107, 197
MscI TGGCCA 2 cut(s) 107, 197
MslI CAYNNNNRTG 2 cut(s) 347, 420
Msp20I TGGCCA 2 cut(s) 107, 197
MspR9I CCNGG 1 cut(s) 291
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 4 cut(s) 38, 194, 404, 515
NcoI CCATGG 1 cut(s) 124
NdeII GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
NlaIII CATG 6 cut(s) 50, 103, 128, 139, 331, 439
NlaIV GGNNCC 3 cut(s) 171, 239, 508
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 296
OliI CACNNNNGTG 1 cut(s) 347
PctI GAATGC 1 cut(s) 190
PkrI GCNGC 3 cut(s) 54, 190, 478
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 289
PspEI GGTNACC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 3 cut(s) 171, 239, 508
PspPI GGNCC 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 5 cut(s) 134, 155, 217, 395, 551
RsaNI GTAC 5 cut(s) 133, 154, 216, 394, 550
RseI CAYNNNNRTG 2 cut(s) 347, 420
SatI GCNGC 3 cut(s) 53, 189, 477
Sau3AI GATC 7 cut(s) 24, 69, 115, 330, 373, 385, 469
Sau96I GGNCC 1 cut(s) 460
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 242
SetI ASST 7 cut(s) 57, 97, 168, 303, 312, 360, 504
SmiMI CAYNNNNRTG 2 cut(s) 347, 420
SpeI ACTAGT 1 cut(s) 344
SsiI CCGC 4 cut(s) 30, 188, 294, 367
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 2 cut(s) 80, 283
TaiI ACGT 1 cut(s) 168
TatI WGTACW 2 cut(s) 132, 549
TauI GCSGC 1 cut(s) 191
TscAI CASTG 3 cut(s) 66, 85, 286
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 52, 476
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 552
TspRI CASTG 3 cut(s) 66, 85, 286
XagI CCTNNNNNAGG 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XspI CTAG 1 cut(s) 345
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.