Rorug02G0555100

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013741
Physical Location & Seq
Reverse (-)
68208154 .. 68209832
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0555100.1

Sequence Viewer

Length: 789 bp
ATGGGGGCGATTCCAGTAGTCCGGTTCCCGATCTTCCGGGCTGTGAGAGTGATCGGAGTAGCAGTGGCGGCTCTAGTCCTGACCTGGACTATTCATTTCCGAGGAGGCTTGGCTCTCATCTCTGACAACAAAGATCTCATCTTCAATGTTCATCCAGTGTTGATGGTGATTGGACTCGTAATCTTAAATGGTGAAGCCATGCTAGCATACAAGATGGTGCCAGGAACAAAAAGCTTCAAGAAATTAGTTCACCTTACACTACAATTCCTTGCTTTTTGTTTAAGCATTATCGGTGTATGGGCTGCTTTGAAGTTCCACAATGATAAGGGCATTGACAATTTCTACAGTCTGCATTCTTGGCTGGGTCTAGCTTGCCTGTTCCTTTTTACCATCCAGTGGGCTGCTGGATTTGTTACATATTGGTACCCAGGTGGCTCGAAAAATAGTAGAGCTAGCTTGCTGCCATGGCATGTCTTTTTCGGTGTTTATATATATGCACTCGCTGTTGTTACTGTAACTACGGGTATCCTAGAAAAAGCCACATTTCTTCAAGCCAACCATGTAATATCACGCTATTCAACTGAAGCTTTGCTGATAAATTCTCTGGGATTATTGGTCGTTGCGCTGGGCGGTTTTGTTATTCTCGCGGTTATTGCTCCTGCTAATAAAGGTGACATTTTCAGAGAATCAATAGACCAAAGCGATTCATTAATGCCAGAAGAGGAATTAGAAGATCATGCTGAAGATGGGGCTTCAGCCATGGTGGCACCTCATCTTCCCGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

262

Amino Acids

28.68

Weight (kDa)

6.75

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 1.3e-48 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 3 cut(s) 217, 423, 766
AccB7I CCANNNNNTGG 1 cut(s) 396
AccII CGCG 1 cut(s) 647
AciI CCGC 3 cut(s) 68, 630, 647
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 3 cut(s) 603, 738, 762
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 5 cut(s) 145, 238, 310, 551, 579
AjnI CCWGG 3 cut(s) 83, 220, 427
AluBI AGCT 5 cut(s) 234, 371, 452, 456, 587
AluI AGCT 5 cut(s) 234, 371, 452, 456, 587
ApeKI GCWGC 3 cut(s) 302, 401, 460
ApoI RAATTY 1 cut(s) 598
AseI ATTAAT 1 cut(s) 710
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 625
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 4 cut(s) 178, 203, 242, 683
AsuNHI GCTAGC 2 cut(s) 202, 452
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BanI GGYRCC 3 cut(s) 217, 423, 766
BbvI GCAGC 3 cut(s) 289, 388, 447
BccI CCATC 4 cut(s) 157, 208, 398, 740
BciT130I CCWGG 3 cut(s) 85, 222, 429
BciVI GTATCC 1 cut(s) 536
BcnI CCSGG 1 cut(s) 38
BfaI CTAG 5 cut(s) 74, 203, 368, 453, 530
BfmI CTRYAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 536
BglI GCCNNNNNGGC 1 cut(s) 764
BglII AGATCT 1 cut(s) 133
BisI GCNGC 4 cut(s) 69, 303, 402, 461
BlsI GCNGC 4 cut(s) 70, 304, 403, 462
Bme1390I CCNGG 4 cut(s) 38, 85, 222, 429
BmiI GGNNCC 4 cut(s) 26, 219, 425, 768
BmrFI CCNGG 4 cut(s) 38, 85, 222, 429
BmtI GCTAGC 2 cut(s) 206, 456
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 4 cut(s) 100, 427, 464, 759
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 3 cut(s) 14, 155, 394
BseBI CCWGG 3 cut(s) 85, 222, 429
BseDI CCNNGG 4 cut(s) 100, 427, 464, 759
BseGI GGATG 2 cut(s) 151, 390
BseLI CCNNNNNNNGG 1 cut(s) 396
BseNI ACTGG 3 cut(s) 14, 155, 394
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 289, 388, 447
BseYI CCCAGC 2 cut(s) 361, 625
Bsh1236I CGCG 1 cut(s) 647
BshNI GGYRCC 3 cut(s) 217, 423, 766
BsiSI CCGG 2 cut(s) 22, 37
BslI CCNNNNNNNGG 1 cut(s) 396
BsmI GAATGC 1 cut(s) 352
Bsp143I GATC 4 cut(s) 30, 51, 133, 733
Bsp19I CCATGG 2 cut(s) 464, 759
BspACI CCGC 3 cut(s) 68, 630, 647
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 4 cut(s) 26, 219, 425, 768
BspOI GCTAGC 2 cut(s) 206, 456
BspT107I GGYRCC 3 cut(s) 217, 423, 766
BsrI ACTGG 3 cut(s) 14, 155, 394
BssECI CCNNGG 4 cut(s) 100, 427, 464, 759
BssMI GATC 4 cut(s) 30, 51, 133, 733
BssT1I CCWWGG 2 cut(s) 464, 759
Bst2UI CCWGG 3 cut(s) 85, 222, 429
Bst4CI ACNGT 2 cut(s) 347, 514
Bst6I CTCTTC 1 cut(s) 714
BstC8I GCNNGC 4 cut(s) 204, 373, 454, 458
BstDSI CCRYGG 2 cut(s) 464, 759
BstF5I GGATG 2 cut(s) 151, 390
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 4 cut(s) 33, 54, 136, 736
BstMBI GATC 4 cut(s) 30, 51, 133, 733
BstMWI GCNNNNNNNGC 6 cut(s) 68, 203, 358, 466, 653, 764
BstNI CCWGG 3 cut(s) 85, 222, 429
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 4 cut(s) 36, 83, 220, 427
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 3 cut(s) 289, 388, 447
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 536
BtgI CCRYGG 2 cut(s) 464, 759
BtsCI GGATG 2 cut(s) 151, 390
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 3 cut(s) 69, 162, 401
Cac8I GCNNGC 4 cut(s) 204, 373, 454, 458
CfoI GCGC 1 cut(s) 625
Csp6I GTAC 1 cut(s) 424
CviAII CATG 6 cut(s) 199, 465, 470, 560, 737, 760
CviQI GTAC 1 cut(s) 424
DpnI GATC 4 cut(s) 32, 53, 135, 735
DpnII GATC 4 cut(s) 30, 51, 133, 733
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 464, 759
Eco57I CTGAAG 3 cut(s) 603, 738, 762
EcoRII CCWGG 3 cut(s) 83, 220, 427
EcoT14I CCWWGG 2 cut(s) 464, 759
ErhI CCWWGG 2 cut(s) 464, 759
FaeI CATG 6 cut(s) 202, 468, 473, 563, 740, 763
FatI CATG 6 cut(s) 198, 464, 469, 559, 736, 759
Fnu4HI GCNGC 4 cut(s) 69, 303, 402, 461
FokI GGATG 2 cut(s) 138, 377
Fsp4HI GCNGC 4 cut(s) 69, 303, 402, 461
FspBI CTAG 5 cut(s) 74, 203, 368, 453, 530
GlaI GCGC 1 cut(s) 624
GluI GCNGC 4 cut(s) 69, 303, 402, 461
GsaI CCCAGC 2 cut(s) 365, 629
HapII CCGG 2 cut(s) 22, 37
HhaI GCGC 1 cut(s) 625
Hin1II CATG 6 cut(s) 202, 468, 473, 563, 740, 763
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HindIII AAGCTT 2 cut(s) 232, 585
HinfI GANTC 4 cut(s) 10, 174, 686, 704
HpaII CCGG 2 cut(s) 22, 37
HphI GGTGA 4 cut(s) 178, 203, 242, 683
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 56, 101, 124, 683
Hpy188III TCNNGA 4 cut(s) 28, 79, 238, 779
Hpy8I GTNNAC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 347, 514
HpyCH4V TGCA 2 cut(s) 352, 497
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 203, 358, 466, 653, 764
Hsp92II CATG 6 cut(s) 202, 468, 473, 563, 740, 763
HspAI GCGC 1 cut(s) 623
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 4 cut(s) 30, 51, 133, 733
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 3 cut(s) 289, 388, 447
MaeI CTAG 5 cut(s) 74, 203, 368, 453, 530
MaeIII GTNAC 4 cut(s) 412, 508, 514, 671
MalI GATC 4 cut(s) 32, 53, 135, 735
MboI GATC 4 cut(s) 30, 51, 133, 733
MboII GAAGA 7 cut(s) 25, 133, 539, 731, 743, 755, 767
MflI RGATCY 1 cut(s) 133
MluCI AATT 5 cut(s) 242, 263, 337, 598, 725
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 4 cut(s) 95, 98, 715, 780
MseI TTAA 3 cut(s) 185, 281, 710
MspI CCGG 2 cut(s) 22, 37
MspR9I CCNGG 4 cut(s) 38, 85, 222, 429
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 3 cut(s) 85, 222, 429
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 6 cut(s) 68, 203, 358, 466, 653, 764
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 2 cut(s) 464, 759
NdeII GATC 4 cut(s) 30, 51, 133, 733
NheI GCTAGC 2 cut(s) 202, 452
NlaIII CATG 6 cut(s) 202, 468, 473, 563, 740, 763
NlaIV GGNNCC 4 cut(s) 26, 219, 425, 768
NmuCI GTSAC 1 cut(s) 671
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 3 cut(s) 10, 686, 704
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 4 cut(s) 70, 304, 403, 462
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 710
Psp6I CCWGG 3 cut(s) 83, 220, 427
PspFI CCCAGC 2 cut(s) 361, 625
PspGI CCWGG 3 cut(s) 83, 220, 427
PspN4I GGNNCC 4 cut(s) 26, 219, 425, 768
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SaqAI TTAA 3 cut(s) 185, 281, 710
SatI GCNGC 4 cut(s) 69, 303, 402, 461
Sau3AI GATC 4 cut(s) 30, 51, 133, 733
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 4 cut(s) 38, 85, 222, 429
SfcI CTRYAG 1 cut(s) 343
Sse9I AATT 5 cut(s) 242, 263, 337, 598, 725
SsiI CCGC 3 cut(s) 68, 630, 647
SspMI CTAG 5 cut(s) 74, 203, 368, 453, 530
StyD4I CCNGG 4 cut(s) 36, 83, 220, 427
StyI CCWWGG 2 cut(s) 464, 759
TaaI ACNGT 2 cut(s) 347, 514
TaqI TCGA 1 cut(s) 437
TasI AATT 5 cut(s) 242, 263, 337, 598, 725
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 3 cut(s) 10, 686, 704
Tru1I TTAA 3 cut(s) 185, 281, 710
Tru9I TTAA 3 cut(s) 185, 281, 710
TscAI CASTG 3 cut(s) 69, 162, 401
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 3 cut(s) 302, 401, 460
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 3 cut(s) 83, 140, 696
TspRI CASTG 3 cut(s) 69, 162, 401
Van91I CCANNNNNTGG 1 cut(s) 396
VspI ATTAAT 1 cut(s) 710
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 5 cut(s) 74, 203, 368, 453, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.