pycom03g02920

SufE-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015104
Physical Location & Seq
Forward (+)
2284208 .. 2285350
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02920.1

Sequence Viewer

Length: 1143 bp
ATGTCGTCATCTTCTTCAATCTCATCGTCGTTTCGATTATTCACCACCAAAATCCCTAGCTCAATTCTCAAACCTAAAACCCCAAAACCTCCATCCATCTCTTTCCCCAAAAGATCCATCACTTTCCAAAGAATCCCCACTATATCCTCCGCCTCCCCTCCGCCGCCGTCCGCATCCGCATCGAGCTCCACGTCCCTGCAGCCCGTCGAGGACCTCCCTCCGAAGCTCCAGGAAATCGTCAAGCTCTTCCAGTCTGTACAGCTCCCGAAGGCCAAGTACGAGCAGCTCCTGTTCTACGGCAAGAATTTGAAGCCGTTGGATGATCGATACAAGACGAAGCAGAACAAAGTGGAGGGATGCGTCTCGCAGGTGTGGGTCAGGGCGTATCTTGACTCAGAGAAGAATGTGTTTTTCGAGGCTGATTCCGACTCGGTCCTTACCAAGGGACTCGCTGCTTTGCTAGTTAGCGGGTTATCGGGCCGCCCGGTGGATGAGGTTTTGCGGGTTTCGCCCGATTTCGCGGTGCTTCTGGGGCTGCAGCAGAGCCTGACTCCGTCGAGGAATAATGGGTTTTTGAATATGTTGAGGTTGATGCAAAGGAAGGCGAAGGAGTTGCTTGTGGAGGAAGAAAATGGAGGGGAGAGTGAGCGTTTCGTGGCGAAAATTGAAGATAATGTCGAAAGTTTGGAGCTTGCAGAGGATTCTGAAGTGGGCGGGGGAAAGAATTCGAAATCGGGTTTGACAGTTGATGGTGGGATTGAGGGTGAGGATATTGGATTGAGTTCTAGAGAAAATGTTAAGCAATTGGAACTGGTAGCGAGTGAAACGAGCGAAAATGTGGCAGATTCAGGTGACTTGGGAAGCAGAGGGAAGAGAATTCAGGAGAAATTGATGCGAGAGTTAAGTCCTGTGGAATTGGAAGTGGAAGACATATCTTATCAACATGCCGGGCACGCTGGGGTTAGAGGGAGTGGTGATGGGGAGACGCATTTCAATGTGAGAGTTGTGTCTAAGGAGTTTGAAGGGAAGAGTTTGGTTAAGAGGCATAGGCTTATATATAGTTTGTTGCAAGAAGAATTGCAAAGTGGACTACACGCATTGTCGATTGTGGCGAAAACACCATCTGAAGTGAGTGGAGGGTGA

Protein Analysis

381

Amino Acids

41.4

Weight (kDa)

5.91

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SufE PF02657 81 - 200 5.1e-38 Fe-S metabolism associated domain
BolA PF01722 297 - 376 2.6e-30 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26500
fragaria_vesca FvH4_3g42140
malus_domestica MD11G1039000.v1.1
prunus_persica Prupe.6G029600_v2.0.a1 Prupe.6G029600_v2.0.a1
pyrus_communis pycom03g02920
rosa_chinensis RchiOBHm_Chr5g0075351
rosa_laevigata RLG00000036552
rosa_multiflora Rmu_sc0000770.1_g000033
rosa_roxburghii Rroxscaffold_1G00006180
rosa_rugosa Rorug05G0439000
rosa_samantha Rh5AG495500 Rh5BG517200 Rh5CG540800 Rh5DG532300
rosa_wichuraiana Rw5G045980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccII CGCG 1 cut(s) 521
AclWI GGATC 1 cut(s) 108
AcsI RAATTY 3 cut(s) 304, 724, 876
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 2 cut(s) 258, 278
AfiI CCNNNNNNNGG 2 cut(s) 442, 487
AgsI TTSAA 6 cut(s) 18, 310, 577, 668, 994, 1022
AjiI CACGTC 1 cut(s) 192
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 7 cut(s) 60, 186, 226, 244, 262, 286, 691
AluI AGCT 7 cut(s) 60, 186, 226, 244, 262, 286, 691
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 2 cut(s) 367, 977
AlwI GGATC 1 cut(s) 108
AlwNI CAGNNNCTG 2 cut(s) 289, 547
AoxI GGCC 2 cut(s) 270, 478
ApeKI GCWGC 5 cut(s) 199, 283, 452, 535, 538
ApoI RAATTY 3 cut(s) 304, 724, 876
Asp700I GAANNNNTTC 1 cut(s) 724
AspS9I GGNCC 3 cut(s) 211, 433, 478
AsuC2I CCSGG 2 cut(s) 485, 949
AsuHPI GGTGA 4 cut(s) 34, 776, 863, 986
AsuII TTCGAA 1 cut(s) 728
AvaII GGWCC 2 cut(s) 211, 433
BaeGI GKGCMC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 188
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbsI GAAGAC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 5 cut(s) 211, 295, 439, 522, 550
BccI CCATC 6 cut(s) 100, 104, 125, 743, 971, 1129
BceAI ACGGC 3 cut(s) 151, 298, 313
BcgI CGANNNNNNTGC 4 cut(s) 162, 196, 595, 629
BciT130I CCWGG 1 cut(s) 230
BcnI CCSGG 2 cut(s) 485, 949
BcoDI GTCTC 2 cut(s) 367, 977
BfaI CTAG 3 cut(s) 57, 461, 786
BfmI CTRYAG 2 cut(s) 197, 536
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 7 cut(s) 164, 200, 284, 453, 481, 536, 539
BlsI GCNGC 7 cut(s) 165, 201, 285, 454, 482, 537, 540
Bme1390I CCNGG 3 cut(s) 230, 485, 949
Bme18I GGWCC 2 cut(s) 211, 433
BmgBI CACGTC 1 cut(s) 192
BmgT120I GGNCC 3 cut(s) 211, 433, 478
BmrFI CCNGG 3 cut(s) 230, 485, 949
BmsI GCATC 5 cut(s) 182, 188, 347, 582, 882
BpiI GAAGAC 1 cut(s) 933
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpmI CTGGAG 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 728
BpuMI CCSGG 2 cut(s) 485, 949
Bsa29I ATCGAT 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 441
Bsc4I CCNNNNNNNGG 2 cut(s) 442, 487
Bse1I ACTGG 2 cut(s) 250, 816
BseBI CCWGG 1 cut(s) 230
BseCI ATCGAT 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 5 cut(s) 92, 173, 325, 362, 496
BseLI CCNNNNNNNGG 2 cut(s) 442, 487
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 250, 816
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 5 cut(s) 211, 295, 439, 522, 550
BseYI CCCAGC 1 cut(s) 956
Bsh1236I CGCG 1 cut(s) 521
BshFI GGCC 2 cut(s) 272, 480
BshVI ATCGAT 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 2 cut(s) 485, 948
BslFI GGGAC 2 cut(s) 178, 459
BslI CCNNNNNNNGG 2 cut(s) 442, 487
BsmAI GTCTC 2 cut(s) 367, 977
BsmBI CGTCTC 2 cut(s) 367, 977
BsmFI GGGAC 2 cut(s) 178, 459
BsnI GGCC 2 cut(s) 272, 480
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 2 cut(s) 188, 954
Bsp1407I TGTACA 1 cut(s) 256
Bsp143I GATC 2 cut(s) 113, 322
BspANI GGCC 2 cut(s) 272, 480
BspCNI CTCAG 1 cut(s) 407
BspDI ATCGAT 1 cut(s) 325
BspFNI CGCG 1 cut(s) 521
BspMAI CTGCAG 2 cut(s) 201, 540
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 728
BsrGI TGTACA 1 cut(s) 256
BsrI ACTGG 2 cut(s) 250, 816
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 2 cut(s) 113, 322
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 745
Bst6I CTCTTC 3 cut(s) 251, 866, 1022
BstAUI TGTACA 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 693, 954
BstDEI CTNAG 2 cut(s) 394, 1011
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 5 cut(s) 92, 173, 325, 362, 496
BstFNI CGCG 1 cut(s) 521
BstKTI GATC 2 cut(s) 116, 325
BstMAI GTCTC 2 cut(s) 367, 977
BstMBI GATC 2 cut(s) 113, 322
BstMWI GCNNNNNNNGC 3 cut(s) 508, 532, 953
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 947
BstSCI CCNGG 3 cut(s) 228, 483, 947
BstSFI CTRYAG 2 cut(s) 197, 536
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 5 cut(s) 211, 295, 439, 522, 550
BstV2I GAAGAC 1 cut(s) 933
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
Bsu15I ATCGAT 1 cut(s) 325
BsuRI GGCC 2 cut(s) 272, 480
BsuTUI ATCGAT 1 cut(s) 325
BtrI CACGTC 1 cut(s) 192
BtsCI GGATG 5 cut(s) 92, 173, 325, 362, 496
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 693, 954
CaiI CAGNNNCTG 2 cut(s) 289, 547
Cfr13I GGNCC 3 cut(s) 211, 433, 478
ClaI ATCGAT 1 cut(s) 325
CseI GACGC 2 cut(s) 349, 994
Csp6I GTAC 2 cut(s) 257, 277
CviAII CATG 1 cut(s) 944
CviQI GTAC 2 cut(s) 257, 277
DdeI CTNAG 2 cut(s) 394, 1011
DpnI GATC 2 cut(s) 115, 324
DpnII GATC 2 cut(s) 113, 322
Eam1104I CTCTTC 3 cut(s) 251, 866, 1022
EarI CTCTTC 3 cut(s) 251, 866, 1022
EciI GGCGGA 2 cut(s) 139, 150
Ecl136II GAGCTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 211, 433
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 211
EcoRI GAATTC 2 cut(s) 724, 876
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 441
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 441
Esp3I CGTCTC 2 cut(s) 367, 977
FaeI CATG 1 cut(s) 947
FaiI YATR 8 cut(s) 143, 581, 932, 945, 1047, 1055, 1057, 1059
FaqI GGGAC 2 cut(s) 178, 459
FatI CATG 1 cut(s) 943
FauI CCCGC 3 cut(s) 461, 495, 707
Fnu4HI GCNGC 7 cut(s) 164, 200, 284, 453, 481, 536, 539
FokI GGATG 5 cut(s) 79, 160, 332, 369, 503
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 7 cut(s) 164, 200, 284, 453, 481, 536, 539
FspBI CTAG 3 cut(s) 57, 461, 786
GluI GCNGC 7 cut(s) 164, 200, 284, 453, 481, 536, 539
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 212
HaeIII GGCC 2 cut(s) 272, 480
HapII CCGG 2 cut(s) 485, 948
HgaI GACGC 2 cut(s) 349, 994
Hin1II CATG 1 cut(s) 947
HinfI GANTC 8 cut(s) 132, 392, 422, 428, 447, 550, 701, 845
HpaII CCGG 2 cut(s) 485, 948
HphI GGTGA 4 cut(s) 34, 776, 863, 986
Hpy166II GTNNAC 1 cut(s) 1088
Hpy188I TCNGA 5 cut(s) 222, 397, 427, 706, 1126
Hpy188III TCNNGA 4 cut(s) 265, 389, 786, 881
Hpy8I GTNNAC 1 cut(s) 1088
Hpy99I CGWCG 3 cut(s) 31, 209, 559
HpyAV CCTTC 4 cut(s) 262, 595, 601, 1016
HpyCH4III ACNGT 1 cut(s) 745
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 6 cut(s) 199, 538, 595, 695, 1069, 1081
HpyF10VI GCNNNNNNNGC 3 cut(s) 508, 532, 953
HpyF3I CTNAG 2 cut(s) 394, 1011
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 1 cut(s) 947
Kzo9I GATC 2 cut(s) 113, 322
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 5 cut(s) 191, 231, 267, 291, 688
Lsp1109I GCAGC 5 cut(s) 211, 295, 439, 522, 550
LweI GCATC 5 cut(s) 182, 188, 347, 582, 882
MaeI CTAG 3 cut(s) 57, 461, 786
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 851
MalI GATC 2 cut(s) 115, 324
MboI GATC 2 cut(s) 113, 322
MfeI CAATTG 1 cut(s) 803
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 2 cut(s) 188, 954
MluCI AATT 9 cut(s) 63, 304, 663, 724, 803, 876, 887, 914, 1076
MlyI GAGTC 4 cut(s) 386, 422, 441, 544
MmeI TCCRAC 2 cut(s) 297, 450
MroXI GAANNNNTTC 1 cut(s) 724
MseI TTAA 3 cut(s) 798, 902, 1038
MslI CAYNNNNRTG 1 cut(s) 993
MspI CCGG 2 cut(s) 485, 948
MspR9I CCNGG 3 cut(s) 230, 485, 949
MunI CAATTG 1 cut(s) 803
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 3 cut(s) 508, 532, 953
NciI CCSGG 2 cut(s) 485, 949
NdeII GATC 2 cut(s) 113, 322
NlaIII CATG 1 cut(s) 947
NmuCI GTSAC 1 cut(s) 851
NspI RCATGY 1 cut(s) 947
NspV TTCGAA 1 cut(s) 728
PaqCI CACCTGC 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 2 cut(s) 188, 1109
PdmI GAANNNNTTC 1 cut(s) 724
PfeI GAWTC 4 cut(s) 132, 422, 701, 845
PflFI GACNNNGTC 2 cut(s) 431, 553
PfoI TCCNGGA 1 cut(s) 228
PkrI GCNGC 7 cut(s) 165, 201, 285, 454, 482, 537, 540
PleI GAGTC 4 cut(s) 386, 422, 441, 544
PpsI GAGTC 4 cut(s) 386, 422, 441, 544
PpuMI RGGWCCY 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 1 cut(s) 228
PspPI GGNCC 3 cut(s) 211, 433, 478
PspPPI RGGWCCY 1 cut(s) 211
PstI CTGCAG 2 cut(s) 201, 540
PstNI CAGNNNCTG 2 cut(s) 289, 547
PsuI RGATCY 1 cut(s) 113
PsyI GACNNNGTC 2 cut(s) 431, 553
RsaI GTAC 2 cut(s) 258, 278
RsaNI GTAC 2 cut(s) 257, 277
RseI CAYNNNNRTG 1 cut(s) 993
SacI GAGCTC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 3 cut(s) 798, 902, 1038
SatI GCNGC 7 cut(s) 164, 200, 284, 453, 481, 536, 539
Sau3AI GATC 2 cut(s) 113, 322
Sau96I GGNCC 3 cut(s) 211, 433, 478
SchI GAGTC 4 cut(s) 386, 422, 441, 544
ScrFI CCNGG 3 cut(s) 230, 485, 949
SduI GDGCHC 2 cut(s) 188, 954
SfaNI GCATC 5 cut(s) 182, 188, 347, 582, 882
SfcI CTRYAG 2 cut(s) 197, 536
SfuI TTCGAA 1 cut(s) 728
SinI GGWCC 2 cut(s) 211, 433
SmiMI CAYNNNNRTG 1 cut(s) 993
Sse9I AATT 9 cut(s) 63, 304, 663, 724, 803, 876, 887, 914, 1076
SspMI CTAG 3 cut(s) 57, 461, 786
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 3 cut(s) 228, 483, 947
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 745
TaiI ACGT 1 cut(s) 194
TaqI TCGA 9 cut(s) 34, 182, 207, 325, 414, 557, 678, 728, 1103
TaqII GACCGA 1 cut(s) 421
TasI AATT 9 cut(s) 63, 304, 663, 724, 803, 876, 887, 914, 1076
TatI WGTACW 1 cut(s) 256
TauI GCSGC 2 cut(s) 166, 483
TfiI GAWTC 4 cut(s) 132, 422, 701, 845
Tru1I TTAA 3 cut(s) 798, 902, 1038
Tru9I TTAA 3 cut(s) 798, 902, 1038
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 5 cut(s) 199, 283, 452, 535, 538
Tsp45I GTSAC 1 cut(s) 851
TspGWI ACGGA 1 cut(s) 543
Tth111I GACNNNGTC 2 cut(s) 431, 553
VpaK11BI GGWCC 2 cut(s) 211, 433
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 3 cut(s) 304, 724, 876
XbaI TCTAGA 1 cut(s) 785
XceI RCATGY 1 cut(s) 947
XmnI GAANNNNTTC 1 cut(s) 724
XspI CTAG 3 cut(s) 57, 461, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.