Rh5DG532300

SufE-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015104
Physical Location & Seq
Forward (+)
82020650 .. 82026691
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG532300.1

Sequence Viewer

Length: 1203 bp
ATGTCGTCGTCTTCTTCTATCTCATCCTCGTTTCGATTATTCACAACCAAACTCCCCAACTCTTCTTTCAACCCCAAAACCCTGTCCGCCTTCCCAAAACACCCAAACTTCTCTTCCTTCCGTCGACCCATCTCTTTCGAAAGAATCCCCACCATATCCTCATCCCGATCCCCATCTGCCTTAGCACCATCAGCTTCAATGCCCCTGCAACCCATCGAAGAACTCCCCCCGAAGCTTCGAGAAATTGTCAAGCTCTTCCAGTCCGTACCGGTTCCAAAAGCCAAGTACGAGGAGCTCCTGTACTACGGCAAGAAGCTCAAACCCCTCGAAGATAAGTACAAGACAAAAGAGAACAAGGTCGAAGGTTGTGTGTCGCAGGTTTGGGTCCGAGCCTACCTCGATTCCGACAAGAACGTGTTCTTTGAGGCCGATTCCGACGCGCTTATCTCGAAAGGTCTCGCTGCTCTGCTCGTTAAAGGGCTTTCGGGTGAACCCGTGGAGGAAATTTTGGGGGTCCGACCCGATTTCGCTCTCCATCTTGGGCTTCAGCAGAGCTTATCCCCCGGTAGGAATAATGGGTTTTTGAATATGTTGAAGTTGATGCAGAAAAAGGCTAAGGATTTGGTTAGTGGGTCGGATTCTGTGGATTTGGGATTGAATTCTCAAGTGGGTGGTGAAAAGATTTCGACTTTGGGTTCGGAAATCGATGGTGGCATTGAGGGTTCTAGTGTTGGATTGAGCTCTAATGAAAATGCTGAGCAATTGAGTGGTACTAGTGGAAAGAATTCGACTTTGGGTTTGAAAATCAATGGTGGCATTGAGAGTGTGAATGTTGAATCGAGGTCAGAAGGAAATGTGAAGGAATTGGAGGTGGTAGGGAGTGTTTCGAATGAAGAGGAGTCGAATTCGGGTGATTTGGGGGGTCGAGGGAAGAGAATTAGGGAAGCATTGGCGAGAGAGCTTAGTCCTGTGGAATTGGAAGTAGAGGATATCTCTTACCAACACGCCGGGCATGCCGGAGTTAGAGGAAGTGGAGATGGGGAGACGCATTTCAATGTGAGAGTTGTGACTAAGGAGTTTGAAGGGAAGAGTATGATCAAGAGGTATAGGCTCATCTACGGCTTGTTGCAAGAGGAGTTGCAGAGTGGACTACATGCATTGTCTATCGAGGCGAAGACGCCAGCTGAAGTGAGTACAAAGTGA

Protein Analysis

400

Amino Acids

43.27

Weight (kDa)

6.38

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SufE PF02657 84 - 203 6.5e-39 Fe-S metabolism associated domain
BolA PF01722 317 - 396 8.5e-28 BolA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26500
fragaria_vesca FvH4_3g42140
malus_domestica MD11G1039000.v1.1
prunus_persica Prupe.6G029600_v2.0.a1 Prupe.6G029600_v2.0.a1
pyrus_communis pycom03g02920
rosa_chinensis RchiOBHm_Chr5g0075351
rosa_laevigata RLG00000036552
rosa_multiflora Rmu_sc0000770.1_g000033
rosa_roxburghii Rroxscaffold_1G00006180
rosa_rugosa Rorug05G0439000
rosa_samantha Rh5AG495500 Rh5BG517200 Rh5CG540800 Rh5DG532300
rosa_wichuraiana Rw5G045980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 367
AccI GTMKAC 1 cut(s) 124
AccII CGCG 1 cut(s) 440
AciI CCGC 1 cut(s) 87
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 4 cut(s) 504, 658, 784, 904
AcuI CTGAAG 1 cut(s) 530
AcyI GRCGYC 1 cut(s) 1178
AfaI GTAC 6 cut(s) 267, 287, 302, 338, 772, 1195
AfiI CCNNNNNNNGG 1 cut(s) 567
AflIII ACRYGT 1 cut(s) 414
AgeI ACCGGT 1 cut(s) 268
AgsI TTSAA 9 cut(s) 70, 198, 586, 595, 658, 802, 836, 1054, 1082
AhlI ACTAGT 1 cut(s) 773
AjuI GAANNNNNNNTTGG 6 cut(s) 50, 82, 97, 129, 776, 808
AluBI AGCT 9 cut(s) 194, 235, 253, 295, 316, 555, 741, 961, 1184
AluI AGCT 9 cut(s) 194, 235, 253, 295, 316, 555, 741, 961, 1184
Alw21I GWGCWC 2 cut(s) 297, 743
Alw26I GTCTC 2 cut(s) 461, 1037
AlwI GGATC 1 cut(s) 162
AoxI GGCC 1 cut(s) 426
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 4 cut(s) 504, 658, 784, 904
ArsI GACNNNNNNTTYG 4 cut(s) 679, 711, 781, 813
AsiGI ACCGGT 1 cut(s) 268
Asp700I GAANNNNTTC 2 cut(s) 416, 784
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 2 cut(s) 385, 514
AsuC2I CCSGG 2 cut(s) 564, 1009
AsuHPI GGTGA 3 cut(s) 500, 686, 923
AsuII TTCGAA 2 cut(s) 138, 887
AvaII GGWCC 2 cut(s) 385, 514
BanII GRGCYC 2 cut(s) 297, 743
BbsI GAAGAC 2 cut(s) 3, 1181
Bbv12I GWGCWC 2 cut(s) 297, 743
BbvI GCAGC 1 cut(s) 448
BccI CCATC 7 cut(s) 137, 181, 196, 221, 543, 701, 1031
BceAI ACGGC 2 cut(s) 322, 1135
BclI TGATCA 1 cut(s) 1095
BcnI CCSGG 2 cut(s) 564, 1009
BcoDI GTCTC 2 cut(s) 461, 1037
BcuI ACTAGT 1 cut(s) 773
BfaI CTAG 2 cut(s) 726, 774
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 1 cut(s) 462
BlpI GCTNAGC 1 cut(s) 756
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 564, 1009
Bme18I GGWCC 2 cut(s) 385, 514
BmgT120I GGNCC 2 cut(s) 385, 514
BmiI GGNNCC 3 cut(s) 273, 386, 515
BmrFI CCNGG 2 cut(s) 564, 1009
BmsI GCATC 1 cut(s) 591
BpiI GAAGAC 2 cut(s) 3, 1181
BplI GAGNNNNNCTC 4 cut(s) 381, 413, 977, 1009
Bpu10I CCTNAGC 2 cut(s) 181, 615
Bpu1102I GCTNAGC 1 cut(s) 756
Bpu14I TTCGAA 2 cut(s) 138, 887
BpuEI CTTGAG 1 cut(s) 648
BpuMI CCSGG 2 cut(s) 564, 1009
Bsa29I ATCGAT 1 cut(s) 705
BsaBI GATNNNNATC 1 cut(s) 172
BsaHI GRCGYC 1 cut(s) 1178
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 495, 562
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 567
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 172
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 495, 562
BseGI GGATG 2 cut(s) 23, 161
BseJI GATNNNNATC 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 567
BseMII CTCAG 1 cut(s) 747
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 3 cut(s) 305, 911, 1148
BseXI GCAGC 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 1 cut(s) 428
BshTI ACCGGT 1 cut(s) 268
BshVI ATCGAT 1 cut(s) 705
BsiHKAI GWGCWC 2 cut(s) 297, 743
BsiSI CCGG 4 cut(s) 269, 564, 1008, 1017
BslI CCNNNNNNNGG 1 cut(s) 567
BsmAI GTCTC 2 cut(s) 461, 1037
BsmBI CGTCTC 1 cut(s) 1037
BsnI GGCC 1 cut(s) 428
Bso31I GGTCTC 1 cut(s) 461
Bsp119I TTCGAA 2 cut(s) 138, 887
Bsp1286I GDGCHC 2 cut(s) 297, 743
Bsp143I GATC 2 cut(s) 167, 1095
Bsp1720I GCTNAGC 1 cut(s) 756
BspACI CCGC 1 cut(s) 87
BspANI GGCC 1 cut(s) 428
BspCNI CTCAG 1 cut(s) 748
BspDI ATCGAT 1 cut(s) 705
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 3 cut(s) 273, 386, 515
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 1 cut(s) 162
BspQI GCTCTTC 1 cut(s) 260
BspT104I TTCGAA 2 cut(s) 138, 887
BspTNI GGTCTC 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 495, 562
BssMI GATC 2 cut(s) 167, 1095
BssNI GRCGYC 1 cut(s) 1178
Bst6I CTCTTC 6 cut(s) 67, 118, 260, 888, 926, 1082
BstACI GRCGYC 1 cut(s) 1178
BstBI TTCGAA 2 cut(s) 138, 887
BstC8I GCNNGC 2 cut(s) 1014, 1182
BstDEI CTNAG 5 cut(s) 181, 615, 756, 962, 1071
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 23, 161
BstFNI CGCG 1 cut(s) 440
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 2 cut(s) 170, 1098
BstMAI GTCTC 2 cut(s) 461, 1037
BstMBI GATC 2 cut(s) 167, 1095
BstMWI GCNNNNNNNGC 2 cut(s) 191, 1013
BstNSI RCATGY 2 cut(s) 1016, 1157
BstSCI CCNGG 2 cut(s) 562, 1007
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 1 cut(s) 448
BstV2I GAAGAC 2 cut(s) 3, 1181
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 1 cut(s) 428
BsuTUI ATCGAT 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 495
BtsCI GGATG 2 cut(s) 23, 161
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 2 cut(s) 1014, 1182
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 2 cut(s) 385, 514
ClaI ATCGAT 1 cut(s) 705
CseI GACGC 3 cut(s) 446, 1054, 1186
Csp6I GTAC 6 cut(s) 266, 286, 301, 337, 771, 1194
CspAI ACCGGT 1 cut(s) 268
CviAII CATG 2 cut(s) 1013, 1154
CviQI GTAC 6 cut(s) 266, 286, 301, 337, 771, 1194
DdeI CTNAG 5 cut(s) 181, 615, 756, 962, 1071
DpnI GATC 2 cut(s) 169, 1097
DpnII GATC 2 cut(s) 167, 1095
Eam1104I CTCTTC 6 cut(s) 67, 118, 260, 888, 926, 1082
EarI CTCTTC 6 cut(s) 67, 118, 260, 888, 926, 1082
EciI GGCGGA 1 cut(s) 76
Ecl136II GAGCTC 2 cut(s) 295, 741
Eco24I GRGCYC 2 cut(s) 297, 743
Eco31I GGTCTC 1 cut(s) 461
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 2 cut(s) 385, 514
Eco53kI GAGCTC 2 cut(s) 295, 741
Eco57I CTGAAG 1 cut(s) 530
EcoICRI GAGCTC 2 cut(s) 295, 741
EcoRI GAATTC 3 cut(s) 658, 784, 904
EcoRV GATATC 1 cut(s) 991
EcoT22I ATGCAT 1 cut(s) 1159
EcoT38I GRGCYC 2 cut(s) 297, 743
Esp3I CGTCTC 1 cut(s) 1037
FaeI CATG 2 cut(s) 1016, 1157
FaiI YATR 6 cut(s) 155, 590, 1014, 1094, 1107, 1155
FatI CATG 2 cut(s) 1012, 1153
FbaI TGATCA 1 cut(s) 1095
FblI GTMKAC 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 2 cut(s) 10, 148
FriOI GRGCYC 2 cut(s) 297, 743
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 2 cut(s) 726, 774
GlaI GCGC 1 cut(s) 441
GluI GCNGC 1 cut(s) 462
HaeIII GGCC 1 cut(s) 428
HapII CCGG 4 cut(s) 269, 564, 1008, 1017
HgaI GACGC 3 cut(s) 446, 1054, 1186
HhaI GCGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 1178
Hin1II CATG 2 cut(s) 1016, 1157
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 6 cut(s) 144, 401, 431, 638, 836, 899
HpaII CCGG 4 cut(s) 269, 564, 1008, 1017
HphI GGTGA 3 cut(s) 500, 686, 923
Hpy166II GTNNAC 3 cut(s) 125, 491, 1148
Hpy188I TCNGA 7 cut(s) 389, 406, 436, 518, 637, 700, 847
Hpy188III TCNNGA 4 cut(s) 165, 239, 448, 1099
Hpy8I GTNNAC 3 cut(s) 125, 491, 1148
Hpy99I CGWCG 3 cut(s) 10, 126, 440
HpyAV CCTTC 6 cut(s) 100, 127, 356, 842, 853, 1076
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 5 cut(s) 208, 604, 1129, 1141, 1157
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 1013
HpyF3I CTNAG 5 cut(s) 181, 615, 756, 962, 1071
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 1178
Hsp92II CATG 2 cut(s) 1016, 1157
HspAI GCGC 1 cut(s) 440
Ksp22I TGATCA 1 cut(s) 1095
Kzo9I GATC 2 cut(s) 167, 1095
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 2 cut(s) 292, 300
Lsp1109I GCAGC 1 cut(s) 448
LweI GCATC 1 cut(s) 591
MaeI CTAG 2 cut(s) 726, 774
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 1066
MalI GATC 2 cut(s) 169, 1097
MboI GATC 2 cut(s) 167, 1095
MfeI CAATTG 1 cut(s) 761
MhlI GDGCHC 2 cut(s) 297, 743
MluCI AATT 9 cut(s) 243, 504, 658, 761, 784, 863, 904, 936, 974
MlyI GAGTC 1 cut(s) 908
MmeI TCCRAC 5 cut(s) 429, 459, 541, 615, 712
Mph1103I ATGCAT 1 cut(s) 1159
MroXI GAANNNNTTC 2 cut(s) 416, 784
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 1053
MspA1I CMGCKG 1 cut(s) 1184
MspI CCGG 4 cut(s) 269, 564, 1008, 1017
MspR9I CCNGG 2 cut(s) 564, 1009
MunI CAATTG 1 cut(s) 761
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 191, 1013
NciI CCSGG 2 cut(s) 564, 1009
NdeII GATC 2 cut(s) 167, 1095
NlaIII CATG 2 cut(s) 1016, 1157
NlaIV GGNNCC 3 cut(s) 273, 386, 515
NmuCI GTSAC 1 cut(s) 1066
NsiI ATGCAT 1 cut(s) 1159
NspI RCATGY 2 cut(s) 1016, 1157
NspV TTCGAA 2 cut(s) 138, 887
PaeI GCATGC 1 cut(s) 1016
PciSI GCTCTTC 1 cut(s) 260
PdmI GAANNNNTTC 2 cut(s) 416, 784
PfeI GAWTC 5 cut(s) 144, 401, 431, 638, 836
PinAI ACCGGT 1 cut(s) 268
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 1 cut(s) 907
PpsI GAGTC 1 cut(s) 907
Psp124BI GAGCTC 2 cut(s) 297, 743
PspN4I GGNNCC 3 cut(s) 273, 386, 515
PspPI GGNCC 2 cut(s) 385, 514
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 6 cut(s) 267, 287, 302, 338, 772, 1195
RsaNI GTAC 6 cut(s) 266, 286, 301, 337, 771, 1194
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 2 cut(s) 297, 743
SalI GTCGAC 1 cut(s) 123
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 2 cut(s) 167, 1095
Sau96I GGNCC 2 cut(s) 385, 514
SchI GAGTC 1 cut(s) 908
ScrFI CCNGG 2 cut(s) 564, 1009
SduI GDGCHC 2 cut(s) 297, 743
SfaNI GCATC 1 cut(s) 591
SfuI TTCGAA 2 cut(s) 138, 887
SinI GGWCC 2 cut(s) 385, 514
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
SpeI ACTAGT 1 cut(s) 773
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 9 cut(s) 243, 504, 658, 761, 784, 863, 904, 936, 974
SsiI CCGC 1 cut(s) 87
SspMI CTAG 2 cut(s) 726, 774
SstI GAGCTC 2 cut(s) 297, 743
StyD4I CCNGG 2 cut(s) 562, 1007
TaiI ACGT 1 cut(s) 417
TasI AATT 9 cut(s) 243, 504, 658, 761, 784, 863, 904, 936, 974
TatI WGTACW 3 cut(s) 300, 336, 1193
TfiI GAWTC 5 cut(s) 144, 401, 431, 638, 836
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TseFI GTSAC 1 cut(s) 1066
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 1066
TspDTI ATGAA 2 cut(s) 762, 906
TspGWI ACGGA 2 cut(s) 110, 253
VpaK11BI GGWCC 2 cut(s) 385, 514
XapI RAATTY 4 cut(s) 504, 658, 784, 904
XceI RCATGY 2 cut(s) 1016, 1157
XmiI GTMKAC 1 cut(s) 124
XmnI GAANNNNTTC 2 cut(s) 416, 784
XspI CTAG 2 cut(s) 726, 774
Zsp2I ATGCAT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.