Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0015104

Physical Location & Seq

Genomic Coordinates Forward (+)

8190233 .. 8195362

Gene Structure 5130 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00006180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGAAAGGATAGTGGTCGACGCTCCGTTTCTTCAGGCTATTTTGGCTGGTCCGGATCGTACCGAGGAGGTTTTGTCATGGTTTGCTCATTCTTTTGCCAACGGCGGCAAAGCATTGCTTTTGGCTATCATTCTCTCCAGATGGTGTGGGAAGCAGGCTTCTATCTCTCTTGGCTTTGCACTGGAAGATTTGGAGTTAAGGGTATTCGGTTGCAAGAAGCCCTCCGTCTATTTCATCACCGCGTGTGTTTCTCTGAAATTCCCTGCTTCTACTACCGGAGGTCTGAGAACCAATCATGTCGTCGTCTTCTTCTATCTCATCCTCGTTTCGGTTATTCACTACCAAAATCCCCCACTCTTCTTCTCTAAACTCCAAAACCCTCTCCACCTTCCCAAAACACCCAAACTTCTCTTCCTTCCGTCGACCCATCTCTTTCGAAAGAATCCCCACCATATCCTCATCCCGATCCCCATCTGCCTTAGCACCATCAGCTTCAATGCCCCTGCAACCCATCGAAGAACTCCCCCGAAGCTTCGAGAAATTGTCAAGCTCTTCCAGTCCGTACCGGTTCCAAAAGCCAAGTACGAGGAGCTCCTGTACTACGGCAAGAAGCTCAAACCCCTCGAAGATAAGTACAAGTCAAAAGAGAACAAGGTCGAAGGTTGTGTGTCGCAGGTTTGGGTCCGAGCCTACCTCGATTCCGACAAGAACGTGTTCTTTGAGGCCGATTCCGACGCGCTTATCTCGAAAGGTCTCGCTGCTCTGCTCGTTAAAGGGCTCTCGGGTGAACCCGTGGAGGAAATTTTGAGGGTCCGACCCGATTTCGCGCTCCATCTTGGGCTTCAGCAGAGCTTATCCCCTGGTAGGAATAATGGGTTTTTGAATATGTTGAAGTTGATGCAGAAAAAGGCTAAGGATTTGGTTAGTGGCTCGGATTATGTGGATTTGGGATTGAATTCTCAAGTGGGTGGTGAAAAGATTTCGACTTTGGGTTCGGAAGTCGATGGTGGCATTGAGGGTTCTAGTGTTGGATTGAGCTCTAATGAAAATGCTGAGCAATTGAGTGGTACTAGTGGAAATAATTCGACTTTGGGTTTGAAAATCAATGGTGGCATTGAGAGTGTGAATGTTGAATCGAGGTCAGAAGGAAATGTGAAGGACTTGGAGGTGGTCGGGAGTGTTTCGAATGAAGAGGAGTCGAATTCGGGTGATTTGGGGAGTCGAGGGAAGAGAATTAAGGAAGCATTGGAGAGAGAGCTTAGTCCTGTGGAATTGGAAGTAGAGGATATCTCTTACCAGCACGCCGGGCATGCCGGAGTTAGAGGAAGTGGTGATGGGGAGACGCATTTCAATGTGAGAGTTGTGACTAAGGAGTTTGAAGGGAAGAGTATGATCAAGAGGCATAGGCTCATCTACGGCTTGTTGCAAGAGGAGTTGCAGAGTGGACTACATGCATTGTCTATCGAGGCGAAGACGCCAGCTGAAGTGAGTACAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008047 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0030234 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050790 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BolA SufE

Protein Analysis

499

Amino Acids

54.67

Weight (kDa)

7.69

Isoelectric Point (pI)

43.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SufE	PF02657	183 - 302	1e-38	Fe-S metabolism associated domain
BolA	PF01722	416 - 495	2e-29	BolA-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26500
fragaria_vesca	FvH4_3g42140
malus_domestica	MD11G1039000.v1.1
prunus_persica	Prupe.6G029600_v2.0.a1 Prupe.6G029600_v2.0.a1
pyrus_communis	pycom03g02920
rosa_chinensis	RchiOBHm_Chr5g0075351
rosa_laevigata	RLG00000036552
rosa_multiflora	Rmu_sc0000770.1_g000033
rosa_roxburghii	Rroxscaffold_1G00006180
rosa_rugosa	Rorug05G0439000
rosa_samantha	Rh5AG495500 Rh5BG517200 Rh5CG540800 Rh5DG532300
rosa_wichuraiana	Rw5G045980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	664
AccI	GTMKAC	2 cut(s)	18, 422
AccII	CGCG	3 cut(s)	242, 737, 827
AccIII	TCCGGA	1 cut(s)	52
AciI	CCGC	2 cut(s)	105, 240
AclWI	GGATC	2 cut(s)	63, 460
AcsI	RAATTY	4 cut(s)	257, 801, 955, 1201
AcuI	CTGAAG	2 cut(s)	17, 827
AcyI	GRCGYC	1 cut(s)	1475
AdeI	CACNNNGTG	1 cut(s)	243
AfaI	GTAC	7 cut(s)	61, 564, 584, 599, 635, 1069, 1492
AfiI	CCNNNNNNNGG	2 cut(s)	328, 864
AflIII	ACRYGT	1 cut(s)	711
AgeI	ACCGGT	1 cut(s)	565
AgsI	TTSAA	8 cut(s)	496, 883, 892, 955, 1099, 1133, 1351, 1379
AhlI	ACTAGT	1 cut(s)	1070
AjnI	CCWGG	1 cut(s)	859
AjuI	GAANNNNNNNTTGG	2 cut(s)	395, 427
AluBI	AGCT	9 cut(s)	492, 532, 550, 592, 613, 852, 1038, 1258, 1481
AluI	AGCT	9 cut(s)	492, 532, 550, 592, 613, 852, 1038, 1258, 1481
Alw21I	GWGCWC	2 cut(s)	594, 1040
Alw26I	GTCTC	2 cut(s)	758, 1334
AlwI	GGATC	2 cut(s)	63, 460
Ama87I	CYCGRG	1 cut(s)	781
Aor13HI	TCCGGA	1 cut(s)	52
AoxI	GGCC	1 cut(s)	723
ApeKI	GCWGC	1 cut(s)	758
ApoI	RAATTY	4 cut(s)	257, 801, 955, 1201
ArsI	GACNNNNNNTTYG	4 cut(s)	976, 1008, 1078, 1110
AsiGI	ACCGGT	1 cut(s)	565
Asp700I	GAANNNNTTC	2 cut(s)	713, 1081
AspLEI	GCGC	2 cut(s)	739, 829
AspS9I	GGNCC	3 cut(s)	50, 682, 811
AsuC2I	CCSGG	1 cut(s)	1306
AsuHPI	GGTGA	5 cut(s)	229, 797, 983, 1220, 1343
AsuII	TTCGAA	2 cut(s)	436, 1184
AvaI	CYCGRG	1 cut(s)	781
AvaII	GGWCC	3 cut(s)	50, 682, 811
BanII	GRGCYC	3 cut(s)	594, 780, 1040
BbsI	GAAGAC	2 cut(s)	298, 1478
Bbv12I	GWGCWC	2 cut(s)	594, 1040
BbvI	GCAGC	1 cut(s)	745
BccI	CCATC	8 cut(s)	135, 435, 479, 494, 519, 840, 998, 1328
BceAI	ACGGC	3 cut(s)	118, 619, 1432
BciT130I	CCWGG	1 cut(s)	861
BclI	TGATCA	1 cut(s)	1392
BcnI	CCSGG	1 cut(s)	1306
BcoDI	GTCTC	2 cut(s)	758, 1334
BcuI	ACTAGT	1 cut(s)	1070
BfaI	CTAG	2 cut(s)	1023, 1071
BfuAI	ACCTGC	1 cut(s)	664
BisI	GCNGC	2 cut(s)	106, 759
BlpI	GCTNAGC	1 cut(s)	1053
BlsI	GCNGC	2 cut(s)	107, 760
Bme1390I	CCNGG	2 cut(s)	861, 1306
Bme18I	GGWCC	3 cut(s)	50, 682, 811
BmeT110I	CYCGRG	1 cut(s)	781
BmgT120I	GGNCC	3 cut(s)	50, 682, 811
BmiI	GGNNCC	3 cut(s)	570, 683, 812
BmrFI	CCNGG	2 cut(s)	861, 1306
BmsI	GCATC	1 cut(s)	888
BpiI	GAAGAC	2 cut(s)	298, 1478
BplI	GAGNNNNNCTC	4 cut(s)	678, 710, 1274, 1306
BpmI	CTGGAG	1 cut(s)	121
Bpu10I	CCTNAGC	2 cut(s)	479, 912
Bpu1102I	GCTNAGC	1 cut(s)	1053
Bpu14I	TTCGAA	2 cut(s)	436, 1184
BpuEI	CTTGAG	1 cut(s)	945
BpuMI	CCSGG	1 cut(s)	1306
BsaBI	GATNNNNATC	1 cut(s)	470
BsaHI	GRCGYC	1 cut(s)	1475
BsaI	GGTCTC	1 cut(s)	758
BsaJI	CCNNGG	3 cut(s)	63, 792, 859
BsaWI	WCCGGW	3 cut(s)	52, 275, 565
BsaXI	ACNNNNNCTCC	4 cut(s)	59, 89, 581, 611
Bsc4I	CCNNNNNNNGG	2 cut(s)	328, 864
Bse118I	RCCGGY	1 cut(s)	565
Bse1I	ACTGG	2 cut(s)	186, 556
Bse3DI	GCAATG	1 cut(s)	113
Bse8I	GATNNNNATC	1 cut(s)	470
BseAI	TCCGGA	1 cut(s)	52
BseBI	CCWGG	1 cut(s)	861
BseDI	CCNNGG	3 cut(s)	63, 792, 859
BseGI	GGATG	2 cut(s)	318, 459
BseJI	GATNNNNATC	1 cut(s)	470
BseLI	CCNNNNNNNGG	2 cut(s)	328, 864
BseMI	GCAATG	1 cut(s)	113
BseMII	CTCAG	2 cut(s)	275, 1044
BseNI	ACTGG	2 cut(s)	186, 556
BseRI	GAGGAG	4 cut(s)	80, 602, 1208, 1445
BseXI	GCAGC	1 cut(s)	745
Bsh1236I	CGCG	3 cut(s)	242, 737, 827
BshFI	GGCC	1 cut(s)	725
BshTI	ACCGGT	1 cut(s)	565
BsiHKAI	GWGCWC	2 cut(s)	594, 1040
BsiHKCI	CYCGRG	1 cut(s)	781
BsiSI	CCGG	5 cut(s)	53, 276, 566, 1305, 1314
BslI	CCNNNNNNNGG	2 cut(s)	328, 864
BsmAI	GTCTC	2 cut(s)	758, 1334
BsmBI	CGTCTC	1 cut(s)	1334
BsnI	GGCC	1 cut(s)	725
Bso31I	GGTCTC	1 cut(s)	758
BsoBI	CYCGRG	1 cut(s)	781
Bsp119I	TTCGAA	2 cut(s)	436, 1184
Bsp1286I	GDGCHC	3 cut(s)	594, 780, 1040
Bsp13I	TCCGGA	1 cut(s)	52
Bsp143I	GATC	3 cut(s)	55, 465, 1392
Bsp1720I	GCTNAGC	1 cut(s)	1053
BspACI	CCGC	2 cut(s)	105, 240
BspANI	GGCC	1 cut(s)	725
BspCNI	CTCAG	2 cut(s)	276, 1045
BspEI	TCCGGA	1 cut(s)	52
BspFNI	CGCG	3 cut(s)	242, 737, 827
BspLI	GGNNCC	3 cut(s)	570, 683, 812
BspMI	ACCTGC	1 cut(s)	664
BspPI	GGATC	2 cut(s)	63, 460
BspQI	GCTCTTC	1 cut(s)	557
BspT104I	TTCGAA	2 cut(s)	436, 1184
BspTNI	GGTCTC	1 cut(s)	758
BsrDI	GCAATG	1 cut(s)	113
BsrFI	RCCGGY	1 cut(s)	565
BsrI	ACTGG	2 cut(s)	186, 556
BssAI	RCCGGY	1 cut(s)	565
BssECI	CCNNGG	3 cut(s)	63, 792, 859
BssMI	GATC	3 cut(s)	55, 465, 1392
BssNI	GRCGYC	1 cut(s)	1475
Bst2UI	CCWGG	1 cut(s)	861
Bst6I	CTCTTC	6 cut(s)	362, 416, 557, 1185, 1223, 1379
BstACI	GRCGYC	1 cut(s)	1475
BstBI	TTCGAA	2 cut(s)	436, 1184
BstC8I	GCNNGC	4 cut(s)	156, 1302, 1311, 1479
BstDEI	CTNAG	6 cut(s)	284, 479, 912, 1053, 1259, 1368
BstDSI	CCRYGG	1 cut(s)	792
BstF5I	GGATG	2 cut(s)	318, 459
BstFNI	CGCG	3 cut(s)	242, 737, 827
BstHHI	GCGC	2 cut(s)	739, 829
BstKTI	GATC	3 cut(s)	58, 468, 1395
BstMAI	GTCTC	2 cut(s)	758, 1334
BstMBI	GATC	3 cut(s)	55, 465, 1392
BstMWI	GCNNNNNNNGC	4 cut(s)	44, 489, 1306, 1310
BstNI	CCWGG	1 cut(s)	861
BstNSI	RCATGY	2 cut(s)	1313, 1454
BstSCI	CCNGG	2 cut(s)	859, 1304
BstUI	CGCG	3 cut(s)	242, 737, 827
BstV1I	GCAGC	1 cut(s)	745
BstV2I	GAAGAC	2 cut(s)	298, 1478
BsuRI	GGCC	1 cut(s)	725
BtgI	CCRYGG	1 cut(s)	792
BtsCI	GGATG	2 cut(s)	318, 459
BtsIMutI	CAGTG	1 cut(s)	179
BveI	ACCTGC	1 cut(s)	664
Cac8I	GCNNGC	4 cut(s)	156, 1302, 1311, 1479
CfoI	GCGC	2 cut(s)	739, 829
Cfr10I	RCCGGY	1 cut(s)	565
Cfr13I	GGNCC	3 cut(s)	50, 682, 811
CseI	GACGC	4 cut(s)	29, 743, 1351, 1483
Csp6I	GTAC	7 cut(s)	60, 563, 583, 598, 634, 1068, 1491
CspAI	ACCGGT	1 cut(s)	565
CviAII	CATG	4 cut(s)	78, 296, 1310, 1451
CviQI	GTAC	7 cut(s)	60, 563, 583, 598, 634, 1068, 1491
DdeI	CTNAG	6 cut(s)	284, 479, 912, 1053, 1259, 1368
DpnI	GATC	3 cut(s)	57, 467, 1394
DpnII	GATC	3 cut(s)	55, 465, 1392
DraIII	CACNNNGTG	1 cut(s)	243
Eam1104I	CTCTTC	6 cut(s)	362, 416, 557, 1185, 1223, 1379
EarI	CTCTTC	6 cut(s)	362, 416, 557, 1185, 1223, 1379
Ecl136II	GAGCTC	2 cut(s)	592, 1038
Eco24I	GRGCYC	3 cut(s)	594, 780, 1040
Eco31I	GGTCTC	1 cut(s)	758
Eco32I	GATATC	1 cut(s)	1288
Eco47I	GGWCC	3 cut(s)	50, 682, 811
Eco53kI	GAGCTC	2 cut(s)	592, 1038
Eco57I	CTGAAG	2 cut(s)	17, 827
Eco88I	CYCGRG	1 cut(s)	781
EcoICRI	GAGCTC	2 cut(s)	592, 1038
EcoRI	GAATTC	2 cut(s)	955, 1201
EcoRII	CCWGG	1 cut(s)	859
EcoRV	GATATC	1 cut(s)	1288
EcoT22I	ATGCAT	1 cut(s)	1456
EcoT38I	GRGCYC	3 cut(s)	594, 780, 1040
Esp3I	CGTCTC	1 cut(s)	1334
FaeI	CATG	4 cut(s)	81, 299, 1313, 1454
FaiI	YATR	9 cut(s)	79, 297, 453, 887, 939, 1311, 1391, 1404, 1452
FalI	AAGNNNNNCTT	2 cut(s)	102, 134
FatI	CATG	4 cut(s)	77, 295, 1309, 1450
FbaI	TGATCA	1 cut(s)	1392
FblI	GTMKAC	2 cut(s)	18, 422
Fnu4HI	GCNGC	2 cut(s)	106, 759
FokI	GGATG	2 cut(s)	305, 446
FriOI	GRGCYC	3 cut(s)	594, 780, 1040
Fsp4HI	GCNGC	2 cut(s)	106, 759
FspBI	CTAG	2 cut(s)	1023, 1071
GlaI	GCGC	2 cut(s)	738, 828
GluI	GCNGC	2 cut(s)	106, 759
GsuI	CTGGAG	1 cut(s)	121
HaeIII	GGCC	1 cut(s)	725
HapII	CCGG	5 cut(s)	53, 276, 566, 1305, 1314
HgaI	GACGC	4 cut(s)	29, 743, 1351, 1483
HhaI	GCGC	2 cut(s)	739, 829
Hin1I	GRCGYC	1 cut(s)	1475
Hin1II	CATG	4 cut(s)	81, 299, 1313, 1454
Hin6I	GCGC	2 cut(s)	737, 827
HinP1I	GCGC	2 cut(s)	737, 827
HincII	GTYRAC	2 cut(s)	19, 423
HindII	GTYRAC	2 cut(s)	19, 423
HindIII	AAGCTT	1 cut(s)	530
HinfI	GANTC	6 cut(s)	442, 698, 728, 1133, 1196, 1219
HpaII	CCGG	5 cut(s)	53, 276, 566, 1305, 1314
HphI	GGTGA	5 cut(s)	229, 797, 983, 1220, 1343
Hpy166II	GTNNAC	4 cut(s)	19, 423, 788, 1445
Hpy188I	TCNGA	9 cut(s)	255, 285, 686, 703, 733, 815, 934, 997, 1144
Hpy188III	TCNNGA	7 cut(s)	53, 138, 463, 536, 745, 1174, 1396
Hpy8I	GTNNAC	4 cut(s)	19, 423, 788, 1445
Hpy99I	CGWCG	4 cut(s)	23, 305, 424, 737
HpyAV	CCTTC	6 cut(s)	398, 425, 653, 1139, 1150, 1373
HpyCH4IV	ACGT	1 cut(s)	711
HpyCH4V	TGCA	7 cut(s)	179, 213, 506, 901, 1426, 1438, 1454
HpyF10VI	GCNNNNNNNGC	4 cut(s)	44, 489, 1306, 1310
HpyF3I	CTNAG	6 cut(s)	284, 479, 912, 1053, 1259, 1368
HpySE526I	ACGT	1 cut(s)	711
Hsp92I	GRCGYC	1 cut(s)	1475
Hsp92II	CATG	4 cut(s)	81, 299, 1313, 1454
HspAI	GCGC	2 cut(s)	737, 827
Kpn2I	TCCGGA	1 cut(s)	52
Ksp22I	TGATCA	1 cut(s)	1392
Kzo9I	GATC	3 cut(s)	55, 465, 1392
LguI	GCTCTTC	1 cut(s)	557
LmnI	GCTCC	4 cut(s)	28, 589, 597, 834
Lsp1109I	GCAGC	1 cut(s)	745
LweI	GCATC	1 cut(s)	888
MaeI	CTAG	2 cut(s)	1023, 1071
MaeII	ACGT	1 cut(s)	711
MaeIII	GTNAC	1 cut(s)	1363
MalI	GATC	3 cut(s)	57, 467, 1394
MboI	GATC	3 cut(s)	55, 465, 1392
MfeI	CAATTG	1 cut(s)	1058
MhlI	GDGCHC	3 cut(s)	594, 780, 1040
MluCI	AATT	9 cut(s)	257, 540, 801, 955, 1058, 1081, 1201, 1233, 1271
MlyI	GAGTC	2 cut(s)	1205, 1228
MmeI	TCCRAC	4 cut(s)	726, 756, 838, 1009
Mph1103I	ATGCAT	1 cut(s)	1456
MroI	TCCGGA	1 cut(s)	52
MroXI	GAANNNNTTC	2 cut(s)	713, 1081
MseI	TTAA	3 cut(s)	197, 771, 1236
MslI	CAYNNNNRTG	1 cut(s)	1350
MspA1I	CMGCKG	1 cut(s)	1481
MspI	CCGG	5 cut(s)	53, 276, 566, 1305, 1314
MspR9I	CCNGG	2 cut(s)	861, 1306
MunI	CAATTG	1 cut(s)	1058
MvaI	CCWGG	1 cut(s)	861
MvnI	CGCG	3 cut(s)	242, 737, 827
MwoI	GCNNNNNNNGC	4 cut(s)	44, 489, 1306, 1310
NciI	CCSGG	1 cut(s)	1306
NdeII	GATC	3 cut(s)	55, 465, 1392
NlaIII	CATG	4 cut(s)	81, 299, 1313, 1454
NlaIV	GGNNCC	3 cut(s)	570, 683, 812
NmuCI	GTSAC	1 cut(s)	1363
NsiI	ATGCAT	1 cut(s)	1456
NspI	RCATGY	2 cut(s)	1313, 1454
NspV	TTCGAA	2 cut(s)	436, 1184
PaeI	GCATGC	1 cut(s)	1313
PciSI	GCTCTTC	1 cut(s)	557
PdmI	GAANNNNTTC	2 cut(s)	713, 1081
PfeI	GAWTC	4 cut(s)	442, 698, 728, 1133
PinAI	ACCGGT	1 cut(s)	565
PkrI	GCNGC	2 cut(s)	107, 760
PleI	GAGTC	2 cut(s)	1204, 1227
PpsI	GAGTC	2 cut(s)	1204, 1227
Psp124BI	GAGCTC	2 cut(s)	594, 1040
Psp6I	CCWGG	1 cut(s)	859
PspGI	CCWGG	1 cut(s)	859
PspN4I	GGNNCC	3 cut(s)	570, 683, 812
PspPI	GGNCC	3 cut(s)	50, 682, 811
PvuII	CAGCTG	1 cut(s)	1481
RsaI	GTAC	7 cut(s)	61, 564, 584, 599, 635, 1069, 1492
RsaNI	GTAC	7 cut(s)	60, 563, 583, 598, 634, 1068, 1491
RseI	CAYNNNNRTG	1 cut(s)	1350
SacI	GAGCTC	2 cut(s)	594, 1040
SalI	GTCGAC	2 cut(s)	17, 421
SapI	GCTCTTC	1 cut(s)	557
SaqAI	TTAA	3 cut(s)	197, 771, 1236
SatI	GCNGC	2 cut(s)	106, 759
Sau3AI	GATC	3 cut(s)	55, 465, 1392
Sau96I	GGNCC	3 cut(s)	50, 682, 811
SchI	GAGTC	2 cut(s)	1205, 1228
ScrFI	CCNGG	2 cut(s)	861, 1306
SduI	GDGCHC	3 cut(s)	594, 780, 1040
SfaNI	GCATC	1 cut(s)	888
SfuI	TTCGAA	2 cut(s)	436, 1184
SinI	GGWCC	3 cut(s)	50, 682, 811
SmiMI	CAYNNNNRTG	1 cut(s)	1350
SmlI	CTYRAG	1 cut(s)	960
SmoI	CTYRAG	1 cut(s)	960
SpeI	ACTAGT	1 cut(s)	1070
SphI	GCATGC	1 cut(s)	1313
Sse9I	AATT	9 cut(s)	257, 540, 801, 955, 1058, 1081, 1201, 1233, 1271
SsiI	CCGC	2 cut(s)	105, 240
SspMI	CTAG	2 cut(s)	1023, 1071
SstI	GAGCTC	2 cut(s)	594, 1040
StyD4I	CCNGG	2 cut(s)	859, 1304
TaiI	ACGT	1 cut(s)	714
TasI	AATT	9 cut(s)	257, 540, 801, 955, 1058, 1081, 1201, 1233, 1271
TatI	WGTACW	3 cut(s)	597, 633, 1490
TauI	GCSGC	1 cut(s)	108
TfiI	GAWTC	4 cut(s)	442, 698, 728, 1133
Tru1I	TTAA	3 cut(s)	197, 771, 1236
Tru9I	TTAA	3 cut(s)	197, 771, 1236
TscAI	CASTG	1 cut(s)	186
TseFI	GTSAC	1 cut(s)	1363
TseI	GCWGC	1 cut(s)	758
Tsp45I	GTSAC	1 cut(s)	1363
TspDTI	ATGAA	3 cut(s)	223, 1059, 1203
TspGWI	ACGGA	4 cut(s)	15, 214, 408, 550
TspRI	CASTG	1 cut(s)	186
VpaK11BI	GGWCC	3 cut(s)	50, 682, 811
XapI	RAATTY	4 cut(s)	257, 801, 955, 1201
XceI	RCATGY	2 cut(s)	1313, 1454
XmiI	GTMKAC	2 cut(s)	18, 422
XmnI	GAANNNNTTC	2 cut(s)	713, 1081
XspI	CTAG	2 cut(s)	1023, 1071
Zsp2I	ATGCAT	1 cut(s)	1456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.