pycom03g09040

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010479
Physical Location & Seq
Reverse (-)
7507488 .. 7508206
719 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGCCGAGTCCGAAGATTTCTAGCCGAAGGGGGCAGCCGGAAGAGAAGAACCGCAGGCCGAGACCGCTCTCTGTTCGATCGTCATCGTTCCACGGCCAGTTCACGGATGCGGCACCGCCGCCACAGCTTCGGAGGCCCAAAACGGTGCCGGACATGGCGTCGTTCAGGAACGCAGTCGGAACAACGGTGTCGTCGACGGAATTACGGCCGAAGCTGACGAAGCTGCTCCTTAACGTTACGATCCAGGGGAGCGTCGGGGCCGTACATGTTGTGATGTCGCCGGAATCAAAGGTCGGAGATTTGGTCGCGGCGGCCGTGCGGCAGTACGGCAAGGAAGGTCGCCGTCCAATCTTGCCTTCCGTTGAGCCGTCGTTATTCGACCTCCATTACTCGCAGGAGAAGAAGCTGATGGAGTTGGGATCCAGGAACTTTTTTCTATGCCCAAACGCAATAGCGGTGGGCGGGGCCGGTGGCGGCGGCGGGGGAGCGATGGCGTCGTCCGCTTCGTGTTTGAAAGAGGCGGAGAAAGTTTCACGAACTGGGTTTGCGTGGCTTAAGTTCATGGATTTCTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

20.68

Weight (kDa)

10.24

Isoelectric Point (pI)

70.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 71 - 147 5.8e-28 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 112, 145
AccBSI CCGCTC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 194
AccII CGCG 1 cut(s) 308
AclI AACGTT 1 cut(s) 234
AclWI GGATC 3 cut(s) 235, 414, 427
AcoI YGGCCR 3 cut(s) 94, 206, 312
AcyI GRCGYC 2 cut(s) 158, 494
AfaI GTAC 2 cut(s) 264, 326
AfiI CCNNNNNNNGG 1 cut(s) 103
AflII CTTAAG 1 cut(s) 554
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 514
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 2 cut(s) 243, 422
AjuI GAANNNNNNNTTGG 2 cut(s) 340, 372
AluBI AGCT 4 cut(s) 127, 214, 223, 406
AluI AGCT 4 cut(s) 127, 214, 223, 406
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 3 cut(s) 235, 414, 427
AoxI GGCC 7 cut(s) 56, 94, 134, 206, 258, 312, 465
ApeKI GCWGC 2 cut(s) 34, 223
AspS9I GGNCC 3 cut(s) 135, 258, 465
BamHI GGATCC 1 cut(s) 419
BanI GGYRCC 2 cut(s) 112, 145
BbvI GCAGC 2 cut(s) 46, 210
BccI CCATC 2 cut(s) 403, 484
BceAI ACGGC 7 cut(s) 109, 221, 245, 299, 327, 343, 352
BciT130I CCWGG 2 cut(s) 245, 424
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 21
BfrI CTTAAG 1 cut(s) 554
BisI GCNGC 9 cut(s) 35, 111, 119, 224, 309, 312, 320, 475, 478
BlsI GCNGC 9 cut(s) 36, 112, 120, 225, 310, 313, 321, 476, 479
Bme1390I CCNGG 2 cut(s) 245, 424
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 3 cut(s) 135, 258, 465
BmiI GGNNCC 5 cut(s) 114, 147, 259, 421, 466
BmrFI CCNGG 2 cut(s) 245, 424
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 549
BsaBI GATNNNNATC 1 cut(s) 82
BsaHI GRCGYC 2 cut(s) 158, 494
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 91, 244
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 97, 544
Bse8I GATNNNNATC 1 cut(s) 82
BseBI CCWGG 2 cut(s) 245, 424
BseDI CCNNGG 2 cut(s) 91, 244
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 97, 544
BseX3I CGGCCG 2 cut(s) 206, 312
BseXI GCAGC 2 cut(s) 46, 210
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 3 cut(s) 80, 209, 315
BshFI GGCC 7 cut(s) 58, 96, 136, 208, 260, 314, 467
BshNI GGYRCC 2 cut(s) 112, 145
BsiEI CGRYCG 3 cut(s) 80, 209, 315
BsiSI CCGG 4 cut(s) 38, 149, 281, 468
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 55
BsnI GGCC 7 cut(s) 58, 96, 136, 208, 260, 314, 467
Bso31I GGTCTC 1 cut(s) 55
Bsp143I GATC 3 cut(s) 77, 240, 419
BspANI GGCC 7 cut(s) 58, 96, 136, 208, 260, 314, 467
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 5 cut(s) 114, 147, 259, 421, 466
BspPI GGATC 3 cut(s) 235, 414, 427
BspT107I GGYRCC 2 cut(s) 112, 145
BspTI CTTAAG 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 55
BsrBI CCGCTC 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 2 cut(s) 97, 544
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 2 cut(s) 91, 244
BssMI GATC 3 cut(s) 77, 240, 419
BssNI GRCGYC 2 cut(s) 158, 494
Bst2UI CCWGG 2 cut(s) 245, 424
Bst4CI ACNGT 2 cut(s) 145, 187
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 2 cut(s) 158, 494
BstAFI CTTAAG 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 112
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 3 cut(s) 80, 243, 422
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 3 cut(s) 77, 240, 419
BstMCI CGRYCG 3 cut(s) 80, 209, 315
BstMWI GCNNNNNNNGC 5 cut(s) 64, 124, 133, 220, 500
BstNI CCWGG 2 cut(s) 245, 424
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 243, 422
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 46, 210
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZI CGGCCG 2 cut(s) 206, 312
BsuRI GGCC 7 cut(s) 58, 96, 136, 208, 260, 314, 467
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 503
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 3 cut(s) 135, 258, 465
CseI GACGC 3 cut(s) 147, 241, 483
Csp6I GTAC 2 cut(s) 263, 325
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 3 cut(s) 154, 266, 562
CviQI GTAC 2 cut(s) 263, 325
DpnI GATC 3 cut(s) 79, 242, 421
DpnII GATC 3 cut(s) 77, 240, 419
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 3 cut(s) 94, 206, 312
EagI CGGCCG 2 cut(s) 206, 312
Eam1104I CTCTTC 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 1 cut(s) 536
EclXI CGGCCG 2 cut(s) 206, 312
Eco31I GGTCTC 1 cut(s) 55
Eco52I CGGCCG 2 cut(s) 206, 312
EcoRII CCWGG 2 cut(s) 243, 422
FaeI CATG 3 cut(s) 157, 269, 565
FaiI YATR 4 cut(s) 155, 267, 439, 563
FatI CATG 3 cut(s) 153, 265, 561
FauI CCCGC 2 cut(s) 455, 473
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 9 cut(s) 35, 111, 119, 224, 309, 312, 320, 475, 478
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 9 cut(s) 35, 111, 119, 224, 309, 312, 320, 475, 478
FspBI CTAG 1 cut(s) 21
GluI GCNGC 9 cut(s) 35, 111, 119, 224, 309, 312, 320, 475, 478
HaeIII GGCC 7 cut(s) 58, 96, 136, 208, 260, 314, 467
HapII CCGG 4 cut(s) 38, 149, 281, 468
HgaI GACGC 3 cut(s) 147, 241, 483
Hin1I GRCGYC 2 cut(s) 158, 494
Hin1II CATG 3 cut(s) 157, 269, 565
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 2 cut(s) 7, 284
HpaII CCGG 4 cut(s) 38, 149, 281, 468
Hpy166II GTNNAC 2 cut(s) 102, 195
Hpy188I TCNGA 4 cut(s) 12, 132, 179, 296
Hpy188III TCNNGA 2 cut(s) 166, 534
Hpy8I GTNNAC 2 cut(s) 102, 195
Hpy99I CGWCG 6 cut(s) 163, 196, 199, 257, 373, 499
HpyAV CCTTC 3 cut(s) 21, 329, 366
HpyCH4III ACNGT 2 cut(s) 145, 187
HpyCH4IV ACGT 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 124, 133, 220, 500
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 2 cut(s) 158, 494
Hsp92II CATG 3 cut(s) 157, 269, 565
Kzo9I GATC 3 cut(s) 77, 240, 419
LmnI GCTCC 3 cut(s) 231, 249, 485
Lsp1109I GCAGC 2 cut(s) 46, 210
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 235
MalI GATC 3 cut(s) 79, 242, 421
MbiI CCGCTC 1 cut(s) 67
MboI GATC 3 cut(s) 77, 240, 419
MboII GAAGA 4 cut(s) 25, 53, 58, 412
MflI RGATCY 1 cut(s) 419
MluCI AATT 1 cut(s) 200
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 157, 274
MnlI CCTC 3 cut(s) 126, 392, 511
MseI TTAA 2 cut(s) 231, 555
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 4 cut(s) 38, 149, 281, 468
MspR9I CCNGG 2 cut(s) 245, 424
MvaI CCWGG 2 cut(s) 245, 424
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 5 cut(s) 64, 124, 133, 220, 500
NdeII GATC 3 cut(s) 77, 240, 419
NlaIII CATG 3 cut(s) 157, 269, 565
NlaIV GGNNCC 5 cut(s) 114, 147, 259, 421, 466
NmeAIII GCCGAG 2 cut(s) 30, 84
NspI RCATGY 1 cut(s) 269
PciI ACATGT 1 cut(s) 265
PcsI WCGNNNNNNNCGW 3 cut(s) 191, 312, 503
PfeI GAWTC 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 422
PkrI GCNGC 9 cut(s) 36, 112, 120, 225, 310, 313, 321, 476, 479
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PscI ACATGT 1 cut(s) 265
Psp1406I AACGTT 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 243, 422
PspGI CCWGG 2 cut(s) 243, 422
PspN4I GGNNCC 5 cut(s) 114, 147, 259, 421, 466
PspPI GGNCC 3 cut(s) 135, 258, 465
PsuI RGATCY 1 cut(s) 419
PvuI CGATCG 1 cut(s) 80
RsaI GTAC 2 cut(s) 264, 326
RsaNI GTAC 2 cut(s) 263, 325
SalI GTCGAC 1 cut(s) 193
SaqAI TTAA 2 cut(s) 231, 555
SatI GCNGC 9 cut(s) 35, 111, 119, 224, 309, 312, 320, 475, 478
Sau3AI GATC 3 cut(s) 77, 240, 419
Sau96I GGNCC 3 cut(s) 135, 258, 465
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 245, 424
SetI ASST 8 cut(s) 129, 216, 225, 237, 294, 340, 384, 408
SfaNI GCATC 1 cut(s) 97
SgrDI CGTCGACG 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 1 cut(s) 200
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 243, 422
TaaI ACNGT 2 cut(s) 145, 187
TaiI ACGT 1 cut(s) 237
TaqI TCGA 3 cut(s) 76, 194, 378
TasI AATT 1 cut(s) 200
TauI GCSGC 7 cut(s) 113, 121, 311, 314, 322, 477, 480
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 231, 555
Tru9I TTAA 2 cut(s) 231, 555
TseI GCWGC 2 cut(s) 34, 223
TspDTI ATGAA 1 cut(s) 550
TspGWI ACGGA 3 cut(s) 119, 212, 349
Vha464I CTTAAG 1 cut(s) 554
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 194
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.