pycom03g11750

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006994
Physical Location & Seq
Reverse (-)
11771387 .. 11779277
7891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3063 bp
ATGGCTGCTGCTGCAGTTGAAAATCTCATAAAGTCTAGGGTTTTCTGGAAAAGTTCAAATTCTGTCTTGTTTTCTGGAATAAGAGGAAAAGGGTTTCTAAAAGGTTTGAAGAGTCCTCACTGCTGGATTGGCTTGAAGAGTCCTCACGGCTGGATTTCAGAAGCGGTTTCTTGTGCGCTTAGCCTCTGCATAAGGCTATCTACTTGTGTGCATGTGTTTTCCAAAATGTCCCTGGTCAGTGATATGGATTCGTTTGAGCCAGATGGAAATGCAGCAAATGGGGTGAATGGAGCTAATGGGGCAAATGGAGTGAATGGAGCAAATGGGGTGAATGGAGCTAATGGGGCAGAGGATGCGTTGCCACCCCCACCCCCGGTTATTCCACCGGATGTTGTTCCTTTGCATGTGGAAGCAGATAACATTCCTGAACCTGTTAAGAAAAAACCTGCTCGGGTCCCTATTGCTAGGCGTGGCCTTGGAACCAAGGGAACAAAAATACCCTTGGTCACAAATCACTTCAAAGTGAATGTTACTAATATCGAGGGTTACTTCTTCCATTATAGTGTTTCTGTTGCTTATGAAGATGGGCGTCCGCTTGATGGCAAGGGTGCTGGAAGAAGAATAATTGAAAGGGTGCATGAGACATACCACTCTGAGTTAGGTGGAAAGGACTTTGCCTATGATGGTGAGAAGAGCCTGTTTACTGTTGGATCCCTTCCGCGCAACAAACTTGAATTCGCCGTTGTTTTGGAGGATGTGCCATCAAGCAGGAACAATGGAAACTGTGAACCTGATGGTCCTGGGAGTCCAAATGAGAGTGACCGAAAGAGATTACGGCGTCCAAATCGCTCCAAAACATTTAATGTGGAGATCAGCTATGCTGCAAAAATTCCCATGAAAGCAATTGGAGATGCTCTTCGTGGTCAAGAATCAGAGAATTCTCAAGAAGCTCTGAGAGTCCTGGATATCATATTAAGACAGCATGCATCGAAGCAAGGATGCCTCCTAGTTCGCCAATCATTCTTCCATAATGATCCAAAAAATTTTGCGGATGTTGGAGGTGGTGTTCTTGGGTGCAGAGGATTCCATTCAAGTTTTAGGACCACCCAGGGTGGCTTGTCTCTGAACATTGATGTATCGACTACCATGATCATACAGCCAGGGCCGGTGGTAGACTTTTTAATTGCCAACCAAAGTGTTAGAGATCCTTTCCAACTTGACTGGACAAAGGCTAAAAGAATACTCAAGAATTTGAGGGTCAAAACAAGTCCGTCAAATCAAGAGTTTAAAATAACTGGATTGAGCGAGAAACCATGCCGAGAACAAACTTTTACATTGAGAAACAAGCAAGCAAAGGAAGGGGAAGATGCAGAAATAGAAGTGACTGTTTATGATTATTTTGTTAATCATCGTAATATACAGCTGCGTTACTCGGCTGATCTACCCTGCCTTAATGTTGGGAAACCAAAACGTCCTACTTATATCCCCCTGGAGCTTTGTTCTTTGGTGTCCTTGCAACGTTACACAAAAGCTCTTTCCACCCTTCAAAGAGCTTCGCTGGTGGAGAAATCAAGGCAGAAGCCACAAGAGAGGATGAGTGTTTTGTCTAATGCTTTGAAAATCAATAATTATGATGCTGAACCAATGCTACACTCATGTGGTGTTTCAATTGGTGGTAGCTTTACTCAAGTGGAAGGCCGTGTTCTTCCAGCCCCAAAGCTTAAAGTCGGTAATGGCGATGATTTCTTCCCTCGAAACGGGCGCTGGAATTTCAACAACAAGACACTTGTGAAGCCGACTAAGATAGAAAAGTGGGCTGTTGTTAACTTCTCCGCACGCTGTGATCTAAAAGCCCTTGTCCGTGATCTGATCAAATGTGGAGATATGAAAGGAATTAAAATCGAGGCTCCATTTGATGTGTTTGAAGAGAATCCTCAGTCTAGGCGCGCCCCACCTTTGGTTAGAGTTGAGAGGATGTTTGAGGATATACAGTCCAAACTTCCTGGACAACCACAGTTTCTTCTCTGTTTGCTTCCGGAGAGAAAAAATTCTGCTTTATATGGTCCATGGAAGCGGAAGAATCTGGCTGAGTATGGTATTGTGACACAATGCATTGCTCCCACAAGGGTCAATGATCAATACCTTACAAATGTCCTGTTAAAGATCAATGCAAAGCTTGGTGGGTTAAATTCCTTGTTGGCAGTTGAATATTCTCCTTCTATCCCTGTGGTTTCCAAGGCTCCCACTATCATCCTTGGGATGGATGTGTCCCATGGTTCTCCTGGGCAGTCTGATGTACCCTCAATTGCTGCGGTGGTAAGCTCCAGGCAGTGGCCACTGATTTCTCGCTACAGGGCATCTGTTAGAACACAATCCCCCAAGGTGGAGATGATAGACTCTCTGTACAAACGAGTTTCTGACACAGAGGATGATGGCATTATGAGAGAGCTGCTTCTGGACTTCTATACAAGTTCAGGCAAACAGAAACCAGACCAGATTATCATCTTCAGGGATGGAGTAAGCGAATCTCAATTTAACCAAGTTTTGAACATTGAACTGGATCAAATCATAGAGGCATGCAAGTTCCTGGATGAGACTTGGTCCCCTAAATTTGTGGTGATCATTGCTCAGAAGAACCACCACACAAAATTCTTTCAGCCTCAATCCCCAGATAATGTTCCTCCTGGGACAATTATCGACAACAAAATTTGTCACCCAAGAAACAATGACTTCTATCTATGTGCTCAAGCTGGAATGATTGGGACCACGAGGCCTACTCACTATCATGTTCTGTTAGACGATGTTGGTTTTTCAGCCGATGAGCTGCAGGAACTAGTGCATTCACTTTCATACGTGTACCAGAGAAGTACCACCGCCATTTCCGTAGTTGCCCCCATTTGCTATGCACATTTAGCTGCCACTCAGATGTCACAGTTCATGAAATTTGAAGATGCGTCTGAGACATCTTCAAGCCATGGCGGTCTTACCTCTGCTGGAGCTGTTCCTGTCCCGCAGCTGCCCAGGTTGAAGGAGAATGTGTCCAGTTCCATGTTTTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

1021

Amino Acids

112.75

Weight (kDa)

9.0

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 164 - 325 3.8e-30 N-terminal domain of argonaute
ArgoL1 PF08699 337 - 386 6e-19 Argonaute linker 1 domain
PAZ PF02170 391 - 520 4.7e-31 PAZ domain
ArgoL2 PF16488 530 - 575 2.5e-11 Argonaute linker 2 domain
ArgoMid PF16487 584 - 636 1e-06 Mid domain of argonaute
Piwi PF02171 674 - 981 7.3e-106 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 454
AccB7I CCANNNNNTGG 1 cut(s) 2331
AccI GTMKAC 1 cut(s) 1173
AccII CGCG 2 cut(s) 721, 1947
AccIII TCCGGA 1 cut(s) 2035
AclI AACGTT 1 cut(s) 1519
AclWI GGATC 5 cut(s) 705, 718, 1028, 1199, 2568
AcoI YGGCCR 1 cut(s) 2333
AcuI CTGAAG 1 cut(s) 2491
AcyI GRCGYC 2 cut(s) 589, 838
AdeI CACNNNGTG 1 cut(s) 1841
AfaI GTAC 4 cut(s) 2298, 2405, 2858, 2869
AfiI CCNNNNNNNGG 7 cut(s) 373, 599, 1757, 1957, 2260, 2331, 2383
AflIII ACRYGT 1 cut(s) 2853
AhlI ACTAGT 1 cut(s) 2833
AleI CACNNNNGTG 1 cut(s) 1656
AloI GAACNNNNNNTCC 2 cut(s) 1050, 1082
Alw21I GWGCWC 1 cut(s) 2746
Alw26I GTCTC 4 cut(s) 635, 1125, 2588, 2954
AlwI GGATC 5 cut(s) 705, 718, 1028, 1199, 2568
Ama87I CYCGRG 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 2035
AoxI GGCC 5 cut(s) 472, 1163, 1696, 2333, 2771
AscI GGCGCGCC 1 cut(s) 1945
Asp700I GAANNNNTTC 1 cut(s) 2047
AspLEI GCGC 5 cut(s) 178, 723, 1764, 1947, 1949
AspS9I GGNCC 7 cut(s) 454, 797, 1101, 1163, 2063, 2601, 2763
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 5 cut(s) 295, 340, 698, 2629, 2705
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 6 cut(s) 454, 797, 1101, 2063, 2601, 2763
BalI TGGCCA 1 cut(s) 2335
BamHI GGATCC 1 cut(s) 710
BauI CACGAG 1 cut(s) 2767
Bbv12I GWGCWC 1 cut(s) 2746
BbvI GCAGC 9 cut(s) 284, 868, 1410, 2296, 2436, 2811, 2902, 3003, 3025
BccI CCATC 9 cut(s) 257, 578, 593, 677, 769, 788, 2254, 2426, 2507
BceAI ACGGC 4 cut(s) 163, 725, 851, 1683
BclI TGATCA 4 cut(s) 1149, 1869, 2134, 2619
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 4 cut(s) 635, 1125, 2588, 2954
BcuI ACTAGT 1 cut(s) 2833
BfaI CTAG 5 cut(s) 36, 465, 1007, 1941, 2834
BfmI CTRYAG 3 cut(s) 12, 2350, 2825
BfoI RGCGCY 1 cut(s) 1765
BfuAI ACCTGC 1 cut(s) 454
BlpI GCTNAGC 1 cut(s) 179
Bme18I GGWCC 6 cut(s) 454, 797, 1101, 2063, 2601, 2763
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 7 cut(s) 454, 797, 1101, 1163, 2063, 2601, 2763
BmiI GGNNCC 8 cut(s) 455, 456, 481, 712, 1908, 2241, 2603, 2764
BmsI GCATC 8 cut(s) 343, 901, 989, 995, 1357, 1624, 2366, 2941
BplI GAGNNNNNCTC 2 cut(s) 2761, 2793
BpmI CTGGAG 3 cut(s) 1511, 2308, 3015
Bpu1102I GCTNAGC 1 cut(s) 179
BpuEI CTTGAG 4 cut(s) 927, 1229, 1671, 2730
BpuMI CCSGG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 2854
BsaBI GATNNNNATC 1 cut(s) 2501
BsaHI GRCGYC 2 cut(s) 589, 838
BsaWI WCCGGW 2 cut(s) 385, 2035
BsaXI ACNNNNNCTCC 4 cut(s) 1050, 1080, 3023, 3053
Bsc4I CCNNNNNNNGG 7 cut(s) 373, 599, 1757, 1957, 2260, 2331, 2383
Bse118I RCCGGY 1 cut(s) 1165
Bse1I ACTGG 4 cut(s) 1226, 1300, 2562, 3042
Bse3DI GCAATG 2 cut(s) 2112, 2622
Bse8I GATNNNNATC 1 cut(s) 2501
BseAI TCCGGA 1 cut(s) 2035
BseJI GATNNNNATC 1 cut(s) 2501
BseLI CCNNNNNNNGG 7 cut(s) 373, 599, 1757, 1957, 2260, 2331, 2383
BseMI GCAATG 2 cut(s) 2112, 2622
BseMII CTCAG 7 cut(s) 645, 944, 1949, 2079, 2642, 2936, 2949
BseNI ACTGG 4 cut(s) 1226, 1300, 2562, 3042
BsePI GCGCGC 1 cut(s) 1945
BseXI GCAGC 9 cut(s) 284, 868, 1410, 2296, 2436, 2811, 2902, 3003, 3025
BsgI GTGCAG 1 cut(s) 1096
Bsh1236I CGCG 2 cut(s) 721, 1947
BshFI GGCC 5 cut(s) 474, 1165, 1698, 2335, 2773
BsiHKAI GWGCWC 1 cut(s) 2746
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 4 cut(s) 374, 386, 1166, 2036
BslFI GGGAC 7 cut(s) 214, 440, 2254, 2587, 2701, 2776, 2993
BslI CCNNNNNNNGG 7 cut(s) 373, 599, 1757, 1957, 2260, 2331, 2383
BsmAI GTCTC 4 cut(s) 635, 1125, 2588, 2954
BsmFI GGGAC 7 cut(s) 214, 440, 2254, 2587, 2701, 2776, 2993
BsmI GAATGC 1 cut(s) 2839
BsnI GGCC 5 cut(s) 474, 1165, 1698, 2335, 2773
BsoBI CYCGRG 1 cut(s) 450
Bsp1286I GDGCHC 1 cut(s) 2746
Bsp13I TCCGGA 1 cut(s) 2035
Bsp1407I TGTACA 1 cut(s) 2403
Bsp1720I GCTNAGC 1 cut(s) 179
Bsp19I CCATGG 3 cut(s) 2066, 2272, 2974
BspANI GGCC 5 cut(s) 474, 1165, 1698, 2335, 2773
BspCNI CTCAG 7 cut(s) 646, 945, 1948, 2080, 2641, 2935, 2950
BspEI TCCGGA 1 cut(s) 2035
BspFNI CGCG 2 cut(s) 721, 1947
BspHI TCATGA 1 cut(s) 2937
BspLI GGNNCC 8 cut(s) 455, 456, 481, 712, 1908, 2241, 2603, 2764
BspMAI CTGCAG 2 cut(s) 16, 2829
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 5 cut(s) 705, 718, 1028, 1199, 2568
BspQI GCTCTTC 2 cut(s) 686, 921
BsrDI GCAATG 2 cut(s) 2112, 2622
BsrFI RCCGGY 1 cut(s) 1165
BsrGI TGTACA 1 cut(s) 2403
BsrI ACTGG 4 cut(s) 1226, 1300, 2562, 3042
BssAI RCCGGY 1 cut(s) 1165
BssHII GCGCGC 1 cut(s) 1945
BssNI GRCGYC 2 cut(s) 589, 838
BssSI CACGAG 1 cut(s) 2767
BssT1I CCWWGG 9 cut(s) 475, 483, 501, 2066, 2235, 2254, 2272, 2379, 2974
Bst2BI CACGAG 1 cut(s) 2767
Bst4CI ACNGT 6 cut(s) 706, 785, 1387, 1992, 2016, 2934
Bst6I CTCTTC 5 cut(s) 104, 131, 686, 921, 1920
BstACI GRCGYC 2 cut(s) 589, 838
BstAPI GCANNNNNTGC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 2403
BstBAI YACGTR 1 cut(s) 2854
BstC8I GCNNGC 5 cut(s) 984, 1350, 1837, 1947, 2578
BstDEI CTNAG 9 cut(s) 179, 654, 953, 1800, 1935, 2088, 2628, 2922, 2958
BstDSI CCRYGG 3 cut(s) 2066, 2272, 2974
BstFNI CGCG 2 cut(s) 721, 1947
BstH2I RGCGCY 1 cut(s) 1765
BstHHI GCGC 5 cut(s) 178, 723, 1764, 1947, 1949
BstMAI GTCTC 4 cut(s) 635, 1125, 2588, 2954
BstMWI GCNNNNNNNGC 6 cut(s) 11, 129, 299, 344, 353, 2912
BstNSI RCATGY 4 cut(s) 215, 407, 986, 2580
BstSFI CTRYAG 3 cut(s) 12, 2350, 2825
BstUI CGCG 2 cut(s) 721, 1947
BstV1I GCAGC 9 cut(s) 284, 868, 1410, 2296, 2436, 2811, 2902, 3003, 3025
BstX2I RGATCY 2 cut(s) 710, 1204
BstYI RGATCY 2 cut(s) 710, 1204
BsuRI GGCC 5 cut(s) 474, 1165, 1698, 2335, 2773
BtgI CCRYGG 3 cut(s) 2066, 2272, 2974
BtgZI GCGATG 1 cut(s) 1752
BtsI GCAGTG 2 cut(s) 118, 2336
BtsIMutI CAGTG 4 cut(s) 118, 244, 2336, 2336
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 5 cut(s) 984, 1350, 1837, 1947, 2578
CciI TCATGA 1 cut(s) 2937
CfoI GCGC 5 cut(s) 178, 723, 1764, 1947, 1949
Cfr10I RCCGGY 1 cut(s) 1165
Cfr13I GGNCC 7 cut(s) 454, 797, 1101, 1163, 2063, 2601, 2763
CseI GACGC 3 cut(s) 578, 827, 2943
Csp6I GTAC 4 cut(s) 2297, 2404, 2857, 2868
CviQI GTAC 4 cut(s) 2297, 2404, 2857, 2868
DdeI CTNAG 9 cut(s) 179, 654, 953, 1800, 1935, 2088, 2628, 2922, 2958
DraI TTTAAA 1 cut(s) 1288
DraIII CACNNNGTG 1 cut(s) 1841
EaeI YGGCCR 1 cut(s) 2333
Eam1104I CTCTTC 5 cut(s) 104, 131, 686, 921, 1920
EarI CTCTTC 5 cut(s) 104, 131, 686, 921, 1920
Eco130I CCWWGG 9 cut(s) 475, 483, 501, 2066, 2235, 2254, 2272, 2379, 2974
Eco147I AGGCCT 1 cut(s) 2773
Eco32I GATATC 1 cut(s) 967
Eco47I GGWCC 6 cut(s) 454, 797, 1101, 2063, 2601, 2763
Eco57I CTGAAG 1 cut(s) 2491
Eco88I CYCGRG 1 cut(s) 450
EcoO109I RGGNCCY 1 cut(s) 454
EcoRI GAATTC 2 cut(s) 734, 937
EcoRV GATATC 1 cut(s) 967
EcoT14I CCWWGG 9 cut(s) 475, 483, 501, 2066, 2235, 2254, 2272, 2379, 2974
EcoT22I ATGCAT 2 cut(s) 988, 2114
ErhI CCWWGG 9 cut(s) 475, 483, 501, 2066, 2235, 2254, 2272, 2379, 2974
FaqI GGGAC 7 cut(s) 214, 440, 2254, 2587, 2701, 2776, 2993
FauI CCCGC 1 cut(s) 3018
FbaI TGATCA 4 cut(s) 1149, 1869, 2134, 2619
FblI GTMKAC 1 cut(s) 1173
FspBI CTAG 5 cut(s) 36, 465, 1007, 1941, 2834
GlaI GCGC 5 cut(s) 177, 722, 1763, 1946, 1948
GsuI CTGGAG 3 cut(s) 1511, 2308, 3015
HaeII RGCGCY 1 cut(s) 1765
HaeIII GGCC 5 cut(s) 474, 1165, 1698, 2335, 2773
HapII CCGG 4 cut(s) 374, 386, 1166, 2036
HgaI GACGC 3 cut(s) 578, 827, 2943
HhaI GCGC 5 cut(s) 178, 723, 1764, 1947, 1949
Hin1I GRCGYC 2 cut(s) 589, 838
Hin6I GCGC 5 cut(s) 176, 721, 1762, 1945, 1947
HinP1I GCGC 5 cut(s) 176, 721, 1762, 1945, 1947
HincII GTYRAC 1 cut(s) 1825
HindII GTYRAC 1 cut(s) 1825
HindIII AAGCTT 2 cut(s) 1718, 2174
HpaI GTTAAC 1 cut(s) 1825
HpaII CCGG 4 cut(s) 374, 386, 1166, 2036
HphI GGTGA 5 cut(s) 295, 340, 698, 2629, 2705
Hpy166II GTNNAC 5 cut(s) 702, 788, 1174, 1825, 2857
Hpy8I GTNNAC 5 cut(s) 702, 788, 1174, 1825, 2857
HpyAV CCTTC 6 cut(s) 725, 1352, 1553, 1688, 2226, 3022
HpyCH4III ACNGT 6 cut(s) 706, 785, 1387, 1992, 2016, 2934
HpyCH4IV ACGT 3 cut(s) 1471, 1519, 2853
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 129, 299, 344, 353, 2912
HpyF3I CTNAG 9 cut(s) 179, 654, 953, 1800, 1935, 2088, 2628, 2922, 2958
HpySE526I ACGT 3 cut(s) 1471, 1519, 2853
Hsp92I GRCGYC 2 cut(s) 589, 838
HspAI GCGC 5 cut(s) 176, 721, 1762, 1945, 1947
KflI GGGWCCC 1 cut(s) 454
Kpn2I TCCGGA 1 cut(s) 2035
Ksp22I TGATCA 4 cut(s) 1149, 1869, 2134, 2619
KspAI GTTAAC 1 cut(s) 1825
LguI GCTCTTC 2 cut(s) 686, 921
Lsp1109I GCAGC 9 cut(s) 284, 868, 1410, 2296, 2436, 2811, 2902, 3003, 3025
LweI GCATC 8 cut(s) 343, 901, 989, 995, 1357, 1624, 2366, 2941
MaeI CTAG 5 cut(s) 36, 465, 1007, 1941, 2834
MaeII ACGT 3 cut(s) 1471, 1519, 2853
MfeI CAATTG 3 cut(s) 903, 1668, 2304
MflI RGATCY 2 cut(s) 710, 1204
MhlI GDGCHC 1 cut(s) 2746
MlsI TGGCCA 1 cut(s) 2335
MluNI TGGCCA 1 cut(s) 2335
MlyI GAGTC 5 cut(s) 121, 148, 814, 966, 2390
MmeI TCCRAC 3 cut(s) 688, 1036, 1237
Mox20I TGGCCA 1 cut(s) 2335
Mph1103I ATGCAT 2 cut(s) 988, 2114
MroI TCCGGA 1 cut(s) 2035
MroXI GAANNNNTTC 1 cut(s) 2047
MscI TGGCCA 1 cut(s) 2335
MslI CAYNNNNRTG 4 cut(s) 561, 1656, 2784, 2924
Msp20I TGGCCA 1 cut(s) 2335
MspA1I CMGCKG 2 cut(s) 1423, 3016
MspI CCGG 4 cut(s) 374, 386, 1166, 2036
MunI CAATTG 3 cut(s) 903, 1668, 2304
Mva1269I GAATGC 1 cut(s) 2839
MvnI CGCG 2 cut(s) 721, 1947
MwoI GCNNNNNNNGC 6 cut(s) 11, 129, 299, 344, 353, 2912
NciI CCSGG 1 cut(s) 374
NcoI CCATGG 3 cut(s) 2066, 2272, 2974
NlaIV GGNNCC 8 cut(s) 455, 456, 481, 712, 1908, 2241, 2603, 2764
NmeAIII GCCGAG 2 cut(s) 1343, 1412
NmuCI GTSAC 6 cut(s) 505, 818, 1381, 2101, 2711, 2928
NsiI ATGCAT 2 cut(s) 988, 2114
NspI RCATGY 4 cut(s) 215, 407, 986, 2580
OliI CACNNNNGTG 1 cut(s) 1656
PaeI GCATGC 2 cut(s) 986, 2580
PagI TCATGA 1 cut(s) 2937
PalAI GGCGCGCC 1 cut(s) 1945
PasI CCCWGGG 1 cut(s) 1108
PauI GCGCGC 1 cut(s) 1945
PceI AGGCCT 1 cut(s) 2773
PciSI GCTCTTC 2 cut(s) 686, 921
PcsI WCGNNNNNNNCGW 1 cut(s) 1734
PctI GAATGC 1 cut(s) 2839
PdmI GAANNNNTTC 1 cut(s) 2047
PfeI GAWTC 6 cut(s) 248, 929, 1083, 1930, 2080, 2525
PflFI GACNNNGTC 1 cut(s) 2599
PflMI CCANNNNNTGG 1 cut(s) 2331
PfoI TCCNGGA 3 cut(s) 960, 2002, 2586
PleI GAGTC 5 cut(s) 120, 147, 813, 965, 2390
PpsI GAGTC 5 cut(s) 120, 147, 813, 965, 2390
Ppu21I YACGTR 1 cut(s) 2854
PpuMI RGGWCCY 1 cut(s) 454
Psp1406I AACGTT 1 cut(s) 1519
Psp5II RGGWCCY 1 cut(s) 454
PspN4I GGNNCC 8 cut(s) 455, 456, 481, 712, 1908, 2241, 2603, 2764
PspPI GGNCC 7 cut(s) 454, 797, 1101, 1163, 2063, 2601, 2763
PspPPI RGGWCCY 1 cut(s) 454
PsrI GAACNNNNNNTAC 2 cut(s) 1632, 1664
PstI CTGCAG 2 cut(s) 16, 2829
PsuI RGATCY 2 cut(s) 710, 1204
PsyI GACNNNGTC 1 cut(s) 2599
PteI GCGCGC 1 cut(s) 1945
PvuII CAGCTG 2 cut(s) 1423, 3016
RsaI GTAC 4 cut(s) 2298, 2405, 2858, 2869
RsaNI GTAC 4 cut(s) 2297, 2404, 2857, 2868
RseI CAYNNNNRTG 4 cut(s) 561, 1656, 2784, 2924
SapI GCTCTTC 2 cut(s) 686, 921
Sau96I GGNCC 7 cut(s) 454, 797, 1101, 1163, 2063, 2601, 2763
SchI GAGTC 5 cut(s) 121, 148, 814, 966, 2390
SduI GDGCHC 1 cut(s) 2746
SfaNI GCATC 8 cut(s) 343, 901, 989, 995, 1357, 1624, 2366, 2941
SfcI CTRYAG 3 cut(s) 12, 2350, 2825
SgsI GGCGCGCC 1 cut(s) 1945
SinI GGWCC 6 cut(s) 454, 797, 1101, 2063, 2601, 2763
SmiMI CAYNNNNRTG 4 cut(s) 561, 1656, 2784, 2924
SmlI CTYRAG 4 cut(s) 942, 1244, 1686, 2745
SmoI CTYRAG 4 cut(s) 942, 1244, 1686, 2745
SpeI ACTAGT 1 cut(s) 2833
SphI GCATGC 2 cut(s) 986, 2580
SseBI AGGCCT 1 cut(s) 2773
SspI AATATT 1 cut(s) 2210
SspMI CTAG 5 cut(s) 36, 465, 1007, 1941, 2834
StuI AGGCCT 1 cut(s) 2773
StyI CCWWGG 9 cut(s) 475, 483, 501, 2066, 2235, 2254, 2272, 2379, 2974
TaaI ACNGT 6 cut(s) 706, 785, 1387, 1992, 2016, 2934
TaiI ACGT 3 cut(s) 1474, 1522, 2856
TaqI TCGA 6 cut(s) 540, 989, 1139, 1753, 1902, 2697
TaqII GACCGA 1 cut(s) 837
TatI WGTACW 1 cut(s) 2403
TfiI GAWTC 6 cut(s) 248, 929, 1083, 1930, 2080, 2525
TscAI CASTG 4 cut(s) 125, 244, 2336, 2343
TseFI GTSAC 6 cut(s) 505, 818, 1381, 2101, 2711, 2928
Tsp45I GTSAC 6 cut(s) 505, 818, 1381, 2101, 2711, 2928
TspDTI ATGAA 6 cut(s) 594, 911, 1901, 2838, 2926, 2954
TspGWI ACGGA 3 cut(s) 1260, 1850, 2872
TspRI CASTG 4 cut(s) 125, 244, 2336, 2343
Tth111I GACNNNGTC 1 cut(s) 2599
Van91I CCANNNNNTGG 1 cut(s) 2331
VpaK11BI GGWCC 6 cut(s) 454, 797, 1101, 2063, 2601, 2763
XceI RCATGY 4 cut(s) 215, 407, 986, 2580
XcmI CCANNNNNNNNNTGG 2 cut(s) 229, 2279
XmiI GTMKAC 1 cut(s) 1173
XmnI GAANNNNTTC 1 cut(s) 2047
XspI CTAG 5 cut(s) 36, 465, 1007, 1941, 2834
Zsp2I ATGCAT 2 cut(s) 988, 2114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.