Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0012421

Physical Location & Seq

Genomic Coordinates Forward (+)

12784284 .. 12786391

Gene Structure 2108 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g12310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGGACACCACAAAACTATTATGTCTCTTAAGAACGTGTATTAGTTCCAAGTCGCTGAAGCAAGGCAAGCTCGTCCACCAAAAGATTCTCACTCTAGGCTTACAGAATGACCCTGCTCTTTGCAAAAACCTGATAGACTTTTACTTCTCTTGCCATTTTTATGACTCTGCAAAGCTTGTTTTCCAATCCATTGAAAACCCAACCAGTATTTCCCTGTGGAATGGCCTCATGACAGCTTATACCAAGAACTTCATGTTCAACAAAGCTCTTGACCTCTTTGGCAGTTTGCTGCGCTACCCTTATCTTTGTCCTGATAGTTACACTTATCCAAGTGTCCTTAAGGCCTGTGGTGCATTGGGTGAAGTTAGTTTGGGAAAAATGATCCATAACCATTTGATAAAGATCGGTTTTGTATCAGATATTGTTGTTGCCAGCTCTCTTGTGTGCATGTATGCTAAATGCAACATTTTTGAGTGTGCTATACGGCTGTTTGATGAAATGCCCGAGAGAGATGTGGCATGTTGGAATACTGTAATTTCTTGTTATTATCAAGATGGGCAAGCTCGTAAGGCAATAGACTTGTATGAAAAGATGAGGAATTCTGGATTTATACCCAATTCAGTAACACTTACGACTGTTATTTCATCGTGTGCAAGACTTTCAGATTTAGAGAGAGGTATGGAGATTCACAAGGAGTTGATAAAAAGTCGCTTGGTTTTGGATTCCTTTGTTTGTTCTGCTCTTGTGGATATGTATGGAAAATGCGGATGTTTAGATATGGCAAAACAGGTTTTTGAGGAAATCCCAATAAAGAATGTAATTTCTTGGAATTCTATGACCACAGCATACAGTGTATCAGGTGACAGTAGATCCTGCATCCAACTTTTTAGAAGGATGAATGATGAAGGAACGAGTCCCACGTTAACTACTTTTAGTAGCATACTGTTGGCCTGTTCAAGGTCAGCCCAACTACAGCACGGAAAATTCATACATGCGTTTATTATAAGAAACAATATTGAGGCTGATATCTACATTTACAGCTCGCTCCTAGATCTCTACTTCATGTGTGGAAGTGTCTCATCAGCTAAAAATGTCTTTGAAAAGATGCCGAAGACAAACACAGCTTCTTGGAATGTTATGATTTCTGGATATGTGAAAGTGGGTCATTATTTTGGGGCTCTGGCCACGTATGATGAAATGAAAGTAGCTGGTGTAAGGCCAAATGCCATCACAGTAACTAGTATTTTATCGGCTTGTTCGCAACTTGCAGCACTGGAAAAGGGTAAGGAGATTCACAGAACTCTCATTGATAGTGAGTTGGAAACCAACGAAATAGTGATGGGGGCTCTCCTTGATATGTATGCTAAATGCGGTGTGGTGGATGAAGCACTTAATGTTTTTAATAGATTACCAGATAGAGACCTTGTGTCATGGACCTCCATGATCACTGCATACGGAGCTCATGGTCAAGCTTTAGAAGCTTTAAAGCTTTTTGGTGAAATGCAGCAGTCCTATGCAAAGCCTGATGGAGTTACTCTTCTTGCGGTTCTATCTGCTTGTAGCCACGATCCATTGGAGACCAATTATGGAATTAAACCTAGAGTGGAACATTACTCTTGTTTGATGGATCTTCTTGGACGTGCTGGAAGGTTGAATGAAGCATATCAACTTTTGCAAAGAACTCCAGAAATTAGGGAGGACATTGACTTGTTAAGCACACTATTTTCCGCTTGTCGTTTGCACAAGGATATAGATTTGGGTGTGAAAATCGCAGGGTTGTTAATGGAGAAGAATCCCGATGATCCTTCAACTTTTATTATGTTATCAAATATTTATGCTTCTGTAAAGAAATGGGATGAAGTGCGGAAGGTGAGATTAAAGATGAAAGAATTAGGATTAAGGAAAAATCCGGGCTGTAGCTGGATTGAGATTAACAAGAGGATCCAGCCTTTCTTTGTTGAAGACAAGTCCCACTCACAATCAGAAATGGTATATGAATGTCTCGCAAGTCTTGCCAGTCACATGGAGAGAGATGAGTTGTTTCCAAATAGAGCAGCAGATATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00440 ko00564 ko01100 ko05231 map00440 map00564 map01100 map05231

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

689

Amino Acids

77.22

Weight (kDa)

6.73

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	170 - 219	1.3e-13	PPR repeat family
PPR	PF01535	174 - 203	6.6e-07	PPR repeat
PPR_long	PF17177	209 - 386	7.1e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	271 - 319	7e-07	PPR repeat family
PPR_3	PF13812	272 - 318	6.7e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	373 - 420	1e-11	PPR repeat family
PPR	PF01535	376 - 405	2.4e-06	PPR repeat
PPR_2	PF13041	474 - 521	2.9e-08	PPR repeat family
PPR	PF01535	476 - 503	2e-07	PPR repeat
E_motif	PF20431	581 - 643	3.4e-18	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27110
fragaria_vesca	FvH4_3g28010
malus_domestica	MD03G1167900.v1.1
prunus_persica	Prupe.4G268300_v2.0.a1
pyrus_communis	pycom03g12310
rosa_chinensis	RchiOBHm_Chr3g0448241
rosa_laevigata	RLG00000025934
rosa_multiflora	Rmu_co8274213.1_g000001 Rmu_sc0005582.1_g000002 Rmu_sc0005969.1_g000039 Rmu_sc0005969.1_g000046 Rmu_sc0016487.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426370
rosa_rugosa	Rorug02G0609300
rosa_samantha	Rh3AG009100 Rh3BG008700 Rh3CG008800 Rh3DG009400
rosa_wichuraiana	Rw3G000740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1004
AciI	CCGC	5 cut(s)	765, 1371, 1544, 1728, 1864
AclWI	GGATC	7 cut(s)	376, 864, 1562, 1635, 1796, 1936, 1949
AcoI	YGGCCR	1 cut(s)	1182
AcsI	RAATTY	3 cut(s)	598, 829, 983
AcuI	CTGAAG	1 cut(s)	77
AfiI	CCNNNNNNNGG	1 cut(s)	957
AflII	CTTAAG	2 cut(s)	28, 338
AflIII	ACRYGT	1 cut(s)	35
AgsI	TTSAA	7 cut(s)	194, 259, 957, 1100, 1654, 1809, 1961
AhdI	GACNNNNNGTC	1 cut(s)	1426
AhlI	ACTAGT	1 cut(s)	1238
AjiI	CACGTC	1 cut(s)	1640
AjuI	GAANNNNNNNTTGG	2 cut(s)	193, 225
Alw21I	GWGCWC	1 cut(s)	1462
Alw26I	GTCTC	5 cut(s)	29, 1081, 1413, 1571, 2006
AlwI	GGATC	7 cut(s)	376, 864, 1562, 1635, 1796, 1936, 1949
Ama87I	CYCGRG	1 cut(s)	503
AoxI	GGCC	5 cut(s)	223, 342, 948, 1182, 1217
ApeKI	GCWGC	4 cut(s)	289, 1268, 1504, 2054
ApoI	RAATTY	3 cut(s)	598, 829, 983
AspLEI	GCGC	1 cut(s)	294
AspS9I	GGNCC	1 cut(s)	1434
AsuC2I	CCSGG	1 cut(s)	1911
AsuHPI	GGTGA	4 cut(s)	371, 872, 1508, 1882
AvaI	CYCGRG	1 cut(s)	503
AvaII	GGWCC	1 cut(s)	1434
BalI	TGGCCA	1 cut(s)	1184
BamHI	GGATCC	1 cut(s)	1941
BanII	GRGCYC	3 cut(s)	1180, 1348, 1462
BbsI	GAAGAC	2 cut(s)	1118, 1968
Bbv12I	GWGCWC	1 cut(s)	1462
BbvI	GCAGC	3 cut(s)	276, 1280, 1516
BccI	CCATC	5 cut(s)	548, 1235, 1333, 1520, 1618
BceAI	ACGGC	1 cut(s)	500
BclI	TGATCA	1 cut(s)	1443
BcnI	CCSGG	1 cut(s)	1911
BcoDI	GTCTC	5 cut(s)	29, 1081, 1413, 1571, 2006
BcuI	ACTAGT	1 cut(s)	1238
BfaI	CTAG	4 cut(s)	95, 1049, 1239, 1599
BfmI	CTRYAG	2 cut(s)	971, 1915
BfrI	CTTAAG	2 cut(s)	28, 338
BglII	AGATCT	1 cut(s)	1051
BisI	GCNGC	4 cut(s)	290, 1269, 1505, 2055
BlsI	GCNGC	4 cut(s)	291, 1270, 1506, 2056
Bme1390I	CCNGG	1 cut(s)	1911
Bme18I	GGWCC	1 cut(s)	1434
BmeRI	GACNNNNNGTC	1 cut(s)	1426
BmeT110I	CYCGRG	1 cut(s)	503
BmgBI	CACGTC	1 cut(s)	1640
BmgT120I	GGNCC	1 cut(s)	1434
BmiI	GGNNCC	1 cut(s)	1943
BmrFI	CCNGG	1 cut(s)	1911
BmsI	GCATC	2 cut(s)	885, 1095
BpiI	GAAGAC	2 cut(s)	1118, 1968
BpmI	CTGGAG	1 cut(s)	1668
BpuMI	CCSGG	1 cut(s)	1911
BsaAI	YACGTR	1 cut(s)	1188
BsaBI	GATNNNNATC	1 cut(s)	401
BsaI	GGTCTC	2 cut(s)	1413, 1571
Bsc4I	CCNNNNNNNGG	1 cut(s)	957
Bse1I	ACTGG	3 cut(s)	204, 1278, 2016
Bse8I	GATNNNNATC	1 cut(s)	401
BseGI	GGATG	5 cut(s)	773, 876, 900, 1387, 1861
BseJI	GATNNNNATC	1 cut(s)	401
BseLI	CCNNNNNNNGG	1 cut(s)	957
BseNI	ACTGG	3 cut(s)	204, 1278, 2016
BseXI	GCAGC	3 cut(s)	276, 1280, 1516
BshFI	GGCC	5 cut(s)	225, 344, 950, 1184, 1219
BsiHKAI	GWGCWC	1 cut(s)	1462
BsiHKCI	CYCGRG	1 cut(s)	503
BsiSI	CCGG	1 cut(s)	1910
BslFI	GGGAC	2 cut(s)	900, 1954
BslI	CCNNNNNNNGG	1 cut(s)	957
BsmAI	GTCTC	5 cut(s)	29, 1081, 1413, 1571, 2006
BsmFI	GGGAC	2 cut(s)	900, 1954
BsnI	GGCC	5 cut(s)	225, 344, 950, 1184, 1219
Bso31I	GGTCTC	2 cut(s)	1413, 1571
BsoBI	CYCGRG	1 cut(s)	503
Bsp1286I	GDGCHC	3 cut(s)	1180, 1348, 1462
Bsp143I	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
BspACI	CCGC	5 cut(s)	765, 1371, 1544, 1728, 1864
BspANI	GGCC	5 cut(s)	225, 344, 950, 1184, 1219
BspHI	TCATGA	1 cut(s)	228
BspLI	GGNNCC	1 cut(s)	1943
BspPI	GGATC	7 cut(s)	376, 864, 1562, 1635, 1796, 1936, 1949
BspTI	CTTAAG	2 cut(s)	28, 338
BspTNI	GGTCTC	2 cut(s)	1413, 1571
BsrI	ACTGG	3 cut(s)	204, 1278, 2016
BssMI	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
Bst4CI	ACNGT	6 cut(s)	532, 637, 851, 866, 945, 1234
Bst6I	CTCTTC	1 cut(s)	1542
BstAFI	CTTAAG	2 cut(s)	28, 338
BstAPI	GCANNNNNTGC	1 cut(s)	2012
BstBAI	YACGTR	1 cut(s)	1188
BstC8I	GCNNGC	4 cut(s)	68, 433, 561, 1043
BstENI	CCTNNNNNAGG	1 cut(s)	955
BstF5I	GGATG	5 cut(s)	773, 876, 900, 1387, 1861
BstHHI	GCGC	1 cut(s)	294
BstKTI	GATC	9 cut(s)	384, 405, 872, 1054, 1446, 1570, 1630, 1804, 1944
BstMAI	GTCTC	5 cut(s)	29, 1081, 1413, 1571, 2006
BstMBI	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 350, 569, 1457, 1478, 2012
BstNSI	RCATGY	3 cut(s)	451, 522, 995
BstSCI	CCNGG	1 cut(s)	1909
BstSFI	CTRYAG	2 cut(s)	971, 1915
BstV1I	GCAGC	3 cut(s)	276, 1280, 1516
BstV2I	GAAGAC	2 cut(s)	1118, 1968
BstX2I	RGATCY	4 cut(s)	869, 1051, 1627, 1941
BstXI	CCANNNNNNTGG	1 cut(s)	2023
BstYI	RGATCY	4 cut(s)	869, 1051, 1627, 1941
BsuRI	GGCC	5 cut(s)	225, 344, 950, 1184, 1219
BtrI	CACGTC	1 cut(s)	1640
BtsCI	GGATG	5 cut(s)	773, 876, 900, 1387, 1861
BtsI	GCAGTG	1 cut(s)	1446
BtsIMutI	CAGTG	3 cut(s)	856, 1271, 1446
Cac8I	GCNNGC	4 cut(s)	68, 433, 561, 1043
CciI	TCATGA	1 cut(s)	228
CfoI	GCGC	1 cut(s)	294
Cfr13I	GGNCC	1 cut(s)	1434
DpnI	GATC	9 cut(s)	383, 404, 871, 1053, 1445, 1569, 1629, 1803, 1943
DpnII	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
DraI	TTTAAA	1 cut(s)	1485
DriI	GACNNNNNGTC	1 cut(s)	1426
EaeI	YGGCCR	1 cut(s)	1182
Eam1104I	CTCTTC	1 cut(s)	1542
Eam1105I	GACNNNNNGTC	1 cut(s)	1426
EarI	CTCTTC	1 cut(s)	1542
Ecl136II	GAGCTC	1 cut(s)	1460
Eco147I	AGGCCT	1 cut(s)	344
Eco24I	GRGCYC	3 cut(s)	1180, 1348, 1462
Eco31I	GGTCTC	2 cut(s)	1413, 1571
Eco32I	GATATC	1 cut(s)	1027
Eco47I	GGWCC	1 cut(s)	1434
Eco53kI	GAGCTC	1 cut(s)	1460
Eco57I	CTGAAG	1 cut(s)	77
Eco88I	CYCGRG	1 cut(s)	503
EcoICRI	GAGCTC	1 cut(s)	1460
EcoNI	CCTNNNNNAGG	1 cut(s)	955
EcoRI	GAATTC	2 cut(s)	598, 829
EcoRV	GATATC	1 cut(s)	1027
EcoT38I	GRGCYC	3 cut(s)	1180, 1348, 1462
FaqI	GGGAC	2 cut(s)	900, 1954
FbaI	TGATCA	1 cut(s)	1443
Fnu4HI	GCNGC	4 cut(s)	290, 1269, 1505, 2055
FokI	GGATG	5 cut(s)	780, 863, 907, 1394, 1868
FriOI	GRGCYC	3 cut(s)	1180, 1348, 1462
Fsp4HI	GCNGC	4 cut(s)	290, 1269, 1505, 2055
FspBI	CTAG	4 cut(s)	95, 1049, 1239, 1599
GlaI	GCGC	1 cut(s)	293
GluI	GCNGC	4 cut(s)	290, 1269, 1505, 2055
GsuI	CTGGAG	1 cut(s)	1668
HaeIII	GGCC	5 cut(s)	225, 344, 950, 1184, 1219
HapII	CCGG	1 cut(s)	1910
HhaI	GCGC	1 cut(s)	294
Hin6I	GCGC	1 cut(s)	292
HinP1I	GCGC	1 cut(s)	292
HincII	GTYRAC	1 cut(s)	924
HindII	GTYRAC	1 cut(s)	924
HindIII	AAGCTT	4 cut(s)	173, 1470, 1479, 1487
HinfI	GANTC	7 cut(s)	85, 164, 685, 722, 913, 1291, 1792
HpaI	GTTAAC	1 cut(s)	924
HpaII	CCGG	1 cut(s)	1910
HphI	GGTGA	4 cut(s)	371, 872, 1508, 1882
Hpy166II	GTNNAC	2 cut(s)	76, 924
Hpy188I	TCNGA	3 cut(s)	418, 664, 1984
Hpy188III	TCNNGA	8 cut(s)	229, 269, 311, 551, 603, 1146, 1685, 1796
Hpy8I	GTNNAC	2 cut(s)	76, 924
HpyAV	CCTTC	5 cut(s)	885, 899, 1641, 1815, 1861
HpyCH4III	ACNGT	6 cut(s)	532, 637, 851, 866, 945, 1234
HpyCH4IV	ACGT	4 cut(s)	35, 920, 1187, 1639
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 350, 569, 1457, 1478, 2012
HpySE526I	ACGT	4 cut(s)	35, 920, 1187, 1639
HspAI	GCGC	1 cut(s)	292
Ksp22I	TGATCA	1 cut(s)	1443
KspAI	GTTAAC	1 cut(s)	924
Kzo9I	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
LmnI	GCTCC	2 cut(s)	1050, 1457
Lsp1109I	GCAGC	3 cut(s)	276, 1280, 1516
LweI	GCATC	2 cut(s)	885, 1095
MaeI	CTAG	4 cut(s)	95, 1049, 1239, 1599
MaeII	ACGT	4 cut(s)	35, 920, 1187, 1639
MaeIII	GTNAC	6 cut(s)	317, 622, 860, 1234, 1531, 2018
MalI	GATC	9 cut(s)	383, 404, 871, 1053, 1445, 1569, 1629, 1803, 1943
MboI	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
MboII	GAAGA	5 cut(s)	1123, 1529, 1622, 1801, 1973
MflI	RGATCY	4 cut(s)	869, 1051, 1627, 1941
MhlI	GDGCHC	3 cut(s)	1180, 1348, 1462
MlsI	TGGCCA	1 cut(s)	1184
MluNI	TGGCCA	1 cut(s)	1184
MlyI	GAGTC	2 cut(s)	158, 922
MmeI	TCCRAC	3 cut(s)	503, 904, 1299
MnlI	CCTC	9 cut(s)	236, 284, 588, 668, 790, 1012, 1447, 1690, 1932
Mox20I	TGGCCA	1 cut(s)	1184
MscI	TGGCCA	1 cut(s)	1184
MslI	CAYNNNNRTG	1 cut(s)	159
Msp20I	TGGCCA	1 cut(s)	1184
MspCI	CTTAAG	2 cut(s)	28, 338
MspI	CCGG	1 cut(s)	1910
MspR9I	CCNGG	1 cut(s)	1911
MwoI	GCNNNNNNNGC	6 cut(s)	67, 350, 569, 1457, 1478, 2012
NciI	CCSGG	1 cut(s)	1911
NdeII	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
NlaIV	GGNNCC	1 cut(s)	1943
NmuCI	GTSAC	2 cut(s)	860, 2018
NspI	RCATGY	3 cut(s)	451, 522, 995
PagI	TCATGA	1 cut(s)	228
PceI	AGGCCT	1 cut(s)	344
PcsI	WCGNNNNNNNCGW	1 cut(s)	917
PfeI	GAWTC	5 cut(s)	85, 685, 722, 1291, 1792
PkrI	GCNGC	4 cut(s)	291, 1270, 1506, 2056
PleI	GAGTC	2 cut(s)	158, 921
PpsI	GAGTC	2 cut(s)	158, 921
Ppu21I	YACGTR	1 cut(s)	1188
PsiI	TTATAA	1 cut(s)	1004
Psp124BI	GAGCTC	1 cut(s)	1462
PspN4I	GGNNCC	1 cut(s)	1943
PspPI	GGNCC	1 cut(s)	1434
PsuI	RGATCY	4 cut(s)	869, 1051, 1627, 1941
RseI	CAYNNNNRTG	1 cut(s)	159
SacI	GAGCTC	1 cut(s)	1462
SatI	GCNGC	4 cut(s)	290, 1269, 1505, 2055
Sau3AI	GATC	9 cut(s)	381, 402, 869, 1051, 1443, 1567, 1627, 1801, 1941
Sau96I	GGNCC	1 cut(s)	1434
SchI	GAGTC	2 cut(s)	158, 922
ScrFI	CCNGG	1 cut(s)	1911
SduI	GDGCHC	3 cut(s)	1180, 1348, 1462
SfaNI	GCATC	2 cut(s)	885, 1095
SfcI	CTRYAG	2 cut(s)	971, 1915
SinI	GGWCC	1 cut(s)	1434
SmiMI	CAYNNNNRTG	1 cut(s)	159
SmlI	CTYRAG	2 cut(s)	28, 338
SmoI	CTYRAG	2 cut(s)	28, 338
SpeI	ACTAGT	1 cut(s)	1238
SseBI	AGGCCT	1 cut(s)	344
SsiI	CCGC	5 cut(s)	765, 1371, 1544, 1728, 1864
SspI	AATATT	2 cut(s)	1015, 1831
SspMI	CTAG	4 cut(s)	95, 1049, 1239, 1599
SstI	GAGCTC	1 cut(s)	1462
StuI	AGGCCT	1 cut(s)	344
StyD4I	CCNGG	1 cut(s)	1909
TaaI	ACNGT	6 cut(s)	532, 637, 851, 866, 945, 1234
TaiI	ACGT	4 cut(s)	38, 923, 1190, 1642
TfiI	GAWTC	5 cut(s)	85, 685, 722, 1291, 1792
TscAI	CASTG	3 cut(s)	856, 1278, 1453
TseFI	GTSAC	2 cut(s)	860, 2018
TseI	GCWGC	4 cut(s)	289, 1268, 1504, 2054
Tsp45I	GTSAC	2 cut(s)	860, 2018
TspGWI	ACGGA	2 cut(s)	993, 1470
TspRI	CASTG	3 cut(s)	856, 1278, 1453
Vha464I	CTTAAG	2 cut(s)	28, 338
VpaK11BI	GGWCC	1 cut(s)	1434
XagI	CCTNNNNNAGG	1 cut(s)	955
XapI	RAATTY	3 cut(s)	598, 829, 983
XceI	RCATGY	3 cut(s)	451, 522, 995
XspI	CTAG	4 cut(s)	95, 1049, 1239, 1599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.