Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0012421

Physical Location & Seq

Genomic Coordinates Reverse (-)

49306213 .. 49308563

Gene Structure 2351 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025934

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGGACTCCACCAAACTATTATCCCTCTTAAGAACATGCATCACTTCGAAGTCGCTGAGACCAGGCAAGCTCATCCACCAAAAGATACTCACTCAAGGCTTCCAATACAACACCCTCTTGTGCAAAAACCTCATAGACTTCTACATCTCTTGCCATTTATGCCCTTCTGCAAAGCTTGTTTTCCAATCCATTGAAAACCCATCCAGCATTTCCTTGTGGAATTCTCTCATGACAGGTTACACCAAGAACCTCATGTTCATTGATGCTCTTGAGCTCTATGAAAGGTTACTCTGCTACCCCTATCTGGGTCCTGATAGTTACACCTACCCAAGTGTTCTCAAGGCCTGCGGTGCATTGGGTAGGGTAGGTCTTGGAAAAATGATCCATAACCATTTGATAAAGACTGGTTTCATATCAGATATCGTCGTGGCAAGTTCCCTTGTGGGAATGTATGCGAAATGCAATGTGTTTGATCCTGCAATTCAGCTGTTTGATGAAATGCCTGAAAGAGATGTGGCATGTTGGAATACTGCAATTTCTTGTTATTATCAAAATGGGCAAGCTCAGAAGGCAATGGACTTGTATGAGAAGATGAGGAGTTCTGGCTTTGAGCCTAATTTAGTAACAATCACTACGGTTATTTCATCATGTGCAAGACTTTTGGATTTGGAGAGAGGTAGGGAGATTCACAAGGAGTTGATACAAAATCGATTCATTTTGGATTCCTTTGTTAGTTCTGCTTTGGTAGACATGTATGGGAAATGTGGATGTTTAGATATGGCAAAAGAGATTTTTGAACAAGTCCCAAGAAAGAATGTCATTGCTTGGAATTCCATGATTGCAGCCTATAGTGTAACAGGTGACAGTAAATCTTGTATCGATCTTTTTAGAAGGATGACTGATGAAGGAACTAGTCCCTCTTTGATTACTTTTAGCAGCATTTTGTTGGCTAGTTCAAGATCGGTCCAACTTCAACATGGAAAATTCATGCACGGATATATGATAAGAAACTATGTAGAGGCTGATATCTATGTTTACAGTTCGCTTATTGATCTCTACTTTGTGTGTGGAAGTATCTCGTCTGCTAAAATGGTCTTTGAAAAGACGTCCAAGACAAATGTAGTTTCTTGGAATGTTATGATTTCTGGATATGTGAAAGTGGGAAATTATTTCAGGGCTCTAGGGTTATATGATGAGATGAAAGAAGCTGGTGTAAGGCCAAACGCCATCACGGTAACTAGTATCCTATCGGCTTGTTCACAACTAACAGCACTGGAAAAGGGTAAGGAGATTCACAGAACTGTTGTTGATAGTGAGTTGGAAACCAATGAAATAGTCATGGGGGCTCTCCTTGACATGTATGCTAAATGTGGTGCGGTGGATGAAGCACGTAATGTTTTCAATAGATTACCAGGTAGAGATCTTGTGTCATGGACATCCATGATCACGGCTTATGGGTCTCATGGTCAGACTAAAGAAGCTATAAAGCTTTTTGATGAAATGCAACAATCCAATGCAAAACCAGATGGAGTTACAATTCTTGCAGTGCTATCCGCATGTAGCCATGTTGGATTAGTTGATGAAGGTTGTTGCTATTTCAATAAAATGATCGGTGAATATGGGATTGAACCGAGAATAGAACATTACTCTTGCTTAATAGATCTTCTTGGCCGTGCTGGTAGGTTGAACGAAGCTTACCAAATCATCCAAAGAACTCCACAAATTAGGGAGGATGTTGACTTGTTAAGTACACTATTTTCTGCATGCCGTTTGCATGGAGATCAAGACTTGGGTGTAAAAATTGCAAGATTGCTAATTGAGAAGAATCCTGATGATCCTTCAACATATATTATGTTATCACATAGTCTTGCTTCTGGAAACAAATGGAATGAGGTGCGGAAGGTGAGACTAAAGATGAAAGAATTGGGATTAAGGAAAAATCCTGGGTGTAGCTGGATTGAGATTAATAAGAGGATTCAGCCTTTCTTTGTTCAAGACGAGTCCCACCCAGAATCCGAAATGAATCTTGAGTACGACTATGCCTTCCTGAATCTTTGTTTAGCACAGTGGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00440 ko00564 ko01100 ko05231 map00440 map00564 map01100 map05231

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

692

Amino Acids

77.72

Weight (kDa)

6.55

Isoelectric Point (pI)

43.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	170 - 219	1.8e-13	PPR repeat family
PPR	PF01535	174 - 203	5.7e-07	PPR repeat
PPR_2	PF13041	271 - 315	1.7e-08	PPR repeat family
PPR_3	PF13812	271 - 315	5e-06	Pentatricopeptide repeat domain
PPR_3	PF13812	371 - 419	1.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	373 - 420	3.2e-14	PPR repeat family
PPR	PF01535	375 - 405	3.5e-09	PPR repeat
PPR_2	PF13041	474 - 521	1.3e-11	PPR repeat family
PPR	PF01535	476 - 504	5.1e-09	PPR repeat
E_motif	PF20431	592 - 654	2.7e-14	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012421)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27110
fragaria_vesca	FvH4_3g28010
malus_domestica	MD03G1167900.v1.1
prunus_persica	Prupe.4G268300_v2.0.a1
pyrus_communis	pycom03g12310
rosa_chinensis	RchiOBHm_Chr3g0448241
rosa_laevigata	RLG00000025934
rosa_multiflora	Rmu_co8274213.1_g000001 Rmu_sc0005582.1_g000002 Rmu_sc0005969.1_g000039 Rmu_sc0005969.1_g000046 Rmu_sc0016487.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426370
rosa_rugosa	Rorug02G0609300
rosa_samantha	Rh3AG009100 Rh3BG008700 Rh3CG008800 Rh3DG009400
rosa_wichuraiana	Rw3G000740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1109
AccI	GTMKAC	1 cut(s)	747
AciI	CCGC	4 cut(s)	348, 1376, 1554, 1897
AclWI	GGATC	3 cut(s)	376, 467, 1829
AcoI	YGGCCR	1 cut(s)	1669
AcsI	RAATTY	3 cut(s)	220, 829, 983
AcyI	GRCGYC	1 cut(s)	1106
AfaI	GTAC	2 cut(s)	1750, 2033
AfiI	CCNNNNNNNGG	2 cut(s)	304, 305
AflII	CTTAAG	1 cut(s)	28
AflIII	ACRYGT	2 cut(s)	750, 1356
AhlI	ACTAGT	2 cut(s)	911, 1238
AjnI	CCWGG	3 cut(s)	61, 1411, 1942
Alw21I	GWGCWC	1 cut(s)	276
Alw26I	GTCTC	3 cut(s)	52, 1464, 1900
AlwI	GGATC	3 cut(s)	376, 467, 1829
AoxI	GGCC	3 cut(s)	342, 1217, 1669
ApeKI	GCWGC	2 cut(s)	842, 936
ApoI	RAATTY	3 cut(s)	220, 829, 983
AseI	ATTAAT	1 cut(s)	1965
AspS9I	GGNCC	2 cut(s)	308, 964
AsuHPI	GGTGA	3 cut(s)	872, 1625, 1915
AsuII	TTCGAA	1 cut(s)	47
AvaII	GGWCC	2 cut(s)	308, 964
BanII	GRGCYC	3 cut(s)	276, 1180, 1348
Bbv12I	GWGCWC	1 cut(s)	276
BbvI	GCAGC	2 cut(s)	854, 948
BccI	CCATC	3 cut(s)	208, 1235, 1520
BceAI	ACGGC	3 cut(s)	1464, 1656, 1752
BciT130I	CCWGG	3 cut(s)	63, 1413, 1944
BciVI	GTATCC	1 cut(s)	1253
BclI	TGATCA	1 cut(s)	1443
BcoDI	GTCTC	3 cut(s)	52, 1464, 1900
BcuI	ACTAGT	2 cut(s)	911, 1238
BfaI	CTAG	4 cut(s)	912, 951, 1181, 1239
BfmI	CTRYAG	1 cut(s)	847
BfrI	CTTAAG	1 cut(s)	28
BfuI	GTATCC	1 cut(s)	1253
BglII	AGATCT	2 cut(s)	1420, 1660
BisI	GCNGC	2 cut(s)	843, 937
BlsI	GCNGC	2 cut(s)	844, 938
Bme1390I	CCNGG	3 cut(s)	63, 1413, 1944
Bme18I	GGWCC	2 cut(s)	308, 964
BmgT120I	GGNCC	2 cut(s)	308, 964
BmiI	GGNNCC	1 cut(s)	309
BmrFI	CCNGG	3 cut(s)	63, 1413, 1944
BmsI	GCATC	2 cut(s)	48, 253
Bpu14I	TTCGAA	1 cut(s)	47
BpuEI	CTTGAG	4 cut(s)	78, 290, 323, 2048
Bsa29I	ATCGAT	2 cut(s)	709, 879
BsaAI	YACGTR	1 cut(s)	1391
BsaHI	GRCGYC	1 cut(s)	1106
BsaI	GGTCTC	2 cut(s)	52, 1464
BsaJI	CCNNGG	1 cut(s)	1943
Bsc4I	CCNNNNNNNGG	2 cut(s)	304, 305
Bse1I	ACTGG	2 cut(s)	409, 1278
Bse3DI	GCAATG	3 cut(s)	469, 579, 819
BseBI	CCWGG	3 cut(s)	63, 1413, 1944
BseCI	ATCGAT	2 cut(s)	709, 879
BseDI	CCNNGG	1 cut(s)	1943
BseGI	GGATG	8 cut(s)	72, 200, 773, 900, 1387, 1436, 1704, 1738
BseLI	CCNNNNNNNGG	2 cut(s)	304, 305
BseMI	GCAATG	3 cut(s)	469, 579, 819
BseMII	CTCAG	2 cut(s)	47, 578
BseNI	ACTGG	2 cut(s)	409, 1278
BseRI	GAGGAG	1 cut(s)	610
BseXI	GCAGC	2 cut(s)	854, 948
BshFI	GGCC	3 cut(s)	344, 1219, 1671
BshVI	ATCGAT	2 cut(s)	709, 879
BsiHKAI	GWGCWC	1 cut(s)	276
BslFI	GGGAC	3 cut(s)	788, 900, 1987
BslI	CCNNNNNNNGG	2 cut(s)	304, 305
BsmAI	GTCTC	3 cut(s)	52, 1464, 1900
BsmFI	GGGAC	3 cut(s)	788, 900, 1987
BsnI	GGCC	3 cut(s)	344, 1219, 1671
Bso31I	GGTCTC	2 cut(s)	52, 1464
Bsp119I	TTCGAA	1 cut(s)	47
Bsp1286I	GDGCHC	3 cut(s)	276, 1180, 1348
BspACI	CCGC	4 cut(s)	348, 1376, 1554, 1897
BspANI	GGCC	3 cut(s)	344, 1219, 1671
BspCNI	CTCAG	2 cut(s)	48, 577
BspDI	ATCGAT	2 cut(s)	709, 879
BspHI	TCATGA	1 cut(s)	228
BspLI	GGNNCC	1 cut(s)	309
BspPI	GGATC	3 cut(s)	376, 467, 1829
BspT104I	TTCGAA	1 cut(s)	47
BspTI	CTTAAG	1 cut(s)	28
BspTNI	GGTCTC	2 cut(s)	52, 1464
BsrDI	GCAATG	3 cut(s)	469, 579, 819
BsrI	ACTGG	2 cut(s)	409, 1278
BssECI	CCNNGG	1 cut(s)	1943
BssNI	GRCGYC	1 cut(s)	1106
Bst2UI	CCWGG	3 cut(s)	63, 1413, 1944
Bst4CI	ACNGT	6 cut(s)	637, 866, 1040, 1234, 1303, 2067
BstACI	GRCGYC	1 cut(s)	1106
BstAFI	CTTAAG	1 cut(s)	28
BstBAI	YACGTR	1 cut(s)	1391
BstBI	TTCGAA	1 cut(s)	47
BstC8I	GCNNGC	4 cut(s)	68, 346, 561, 1765
BstDEI	CTNAG	2 cut(s)	56, 564
BstF5I	GGATG	8 cut(s)	72, 200, 773, 900, 1387, 1436, 1704, 1738
BstMAI	GTCTC	3 cut(s)	52, 1464, 1900
BstMWI	GCNNNNNNNGC	3 cut(s)	159, 350, 569
BstNI	CCWGG	3 cut(s)	63, 1413, 1944
BstNSI	RCATGY	6 cut(s)	39, 522, 754, 1360, 1560, 1767
BstSCI	CCNGG	3 cut(s)	61, 1411, 1942
BstSFI	CTRYAG	1 cut(s)	847
BstV1I	GCAGC	2 cut(s)	854, 948
BstX2I	RGATCY	2 cut(s)	1420, 1660
BstYI	RGATCY	2 cut(s)	1420, 1660
Bsu15I	ATCGAT	2 cut(s)	709, 879
BsuI	GTATCC	1 cut(s)	1253
BsuRI	GGCC	3 cut(s)	344, 1219, 1671
BsuTUI	ATCGAT	2 cut(s)	709, 879
BtsCI	GGATG	8 cut(s)	72, 200, 773, 900, 1387, 1436, 1704, 1738
BtsI	GCAGTG	1 cut(s)	1551
BtsIMutI	CAGTG	3 cut(s)	1271, 1551, 2072
Cac8I	GCNNGC	4 cut(s)	68, 346, 561, 1765
CciI	TCATGA	1 cut(s)	228
Cfr13I	GGNCC	2 cut(s)	308, 964
ClaI	ATCGAT	2 cut(s)	709, 879
CsiI	ACCWGGT	1 cut(s)	1411
Csp6I	GTAC	2 cut(s)	1749, 2032
CviQI	GTAC	2 cut(s)	1749, 2032
DdeI	CTNAG	2 cut(s)	56, 564
EaeI	YGGCCR	1 cut(s)	1669
Ecl136II	GAGCTC	1 cut(s)	274
Eco147I	AGGCCT	1 cut(s)	344
Eco24I	GRGCYC	3 cut(s)	276, 1180, 1348
Eco31I	GGTCTC	2 cut(s)	52, 1464
Eco32I	GATATC	2 cut(s)	421, 1027
Eco47I	GGWCC	2 cut(s)	308, 964
Eco53kI	GAGCTC	1 cut(s)	274
EcoICRI	GAGCTC	1 cut(s)	274
EcoO109I	RGGNCCY	1 cut(s)	308
EcoRI	GAATTC	2 cut(s)	220, 829
EcoRII	CCWGG	3 cut(s)	61, 1411, 1942
EcoRV	GATATC	2 cut(s)	421, 1027
EcoT22I	ATGCAT	1 cut(s)	41
EcoT38I	GRGCYC	3 cut(s)	276, 1180, 1348
FaqI	GGGAC	3 cut(s)	788, 900, 1987
FbaI	TGATCA	1 cut(s)	1443
FblI	GTMKAC	1 cut(s)	747
Fnu4HI	GCNGC	2 cut(s)	843, 937
FokI	GGATG	8 cut(s)	59, 187, 780, 907, 1394, 1423, 1691, 1745
FriOI	GRGCYC	3 cut(s)	276, 1180, 1348
Fsp4HI	GCNGC	2 cut(s)	843, 937
FspBI	CTAG	4 cut(s)	912, 951, 1181, 1239
GluI	GCNGC	2 cut(s)	843, 937
HaeIII	GGCC	3 cut(s)	344, 1219, 1671
Hin1I	GRCGYC	1 cut(s)	1106
HincII	GTYRAC	1 cut(s)	1738
HindII	GTYRAC	1 cut(s)	1738
HindIII	AAGCTT	3 cut(s)	173, 1487, 1692
HphI	GGTGA	3 cut(s)	872, 1625, 1915
Hpy166II	GTNNAC	5 cut(s)	748, 1036, 1259, 1738, 1751
Hpy188I	TCNGA	4 cut(s)	418, 567, 1470, 2017
Hpy8I	GTNNAC	5 cut(s)	748, 1036, 1259, 1738, 1751
Hpy99I	CGWCG	1 cut(s)	428
HpyAV	CCTTC	8 cut(s)	174, 562, 885, 899, 1577, 1848, 1894, 2053
HpyCH4III	ACNGT	6 cut(s)	637, 866, 1040, 1234, 1303, 2067
HpyCH4IV	ACGT	2 cut(s)	1106, 1390
HpyF10VI	GCNNNNNNNGC	3 cut(s)	159, 350, 569
HpyF3I	CTNAG	2 cut(s)	56, 564
HpySE526I	ACGT	2 cut(s)	1106, 1390
Hsp92I	GRCGYC	1 cut(s)	1106
Ksp22I	TGATCA	1 cut(s)	1443
Lsp1109I	GCAGC	2 cut(s)	854, 948
LweI	GCATC	2 cut(s)	48, 253
MabI	ACCWGGT	1 cut(s)	1411
MaeI	CTAG	4 cut(s)	912, 951, 1181, 1239
MaeII	ACGT	2 cut(s)	1106, 1390
MaeIII	GTNAC	8 cut(s)	236, 285, 317, 622, 853, 860, 1234, 1531
MboII	GAAGA	3 cut(s)	601, 1655, 1834
MflI	RGATCY	2 cut(s)	1420, 1660
MhlI	GDGCHC	3 cut(s)	276, 1180, 1348
MlyI	GAGTC	1 cut(s)	2009
MmeI	TCCRAC	4 cut(s)	503, 991, 1299, 1549
Mph1103I	ATGCAT	1 cut(s)	41
MseI	TTAA	5 cut(s)	29, 1655, 1745, 1931, 1965
MspA1I	CMGCKG	1 cut(s)	487
MspCI	CTTAAG	1 cut(s)	28
MspR9I	CCNGG	3 cut(s)	63, 1413, 1944
MvaI	CCWGG	3 cut(s)	63, 1413, 1944
MwoI	GCNNNNNNNGC	3 cut(s)	159, 350, 569
NlaIV	GGNNCC	1 cut(s)	309
NmuCI	GTSAC	1 cut(s)	860
NsiI	ATGCAT	1 cut(s)	41
NspI	RCATGY	6 cut(s)	39, 522, 754, 1360, 1560, 1767
NspV	TTCGAA	1 cut(s)	47
PaeI	GCATGC	1 cut(s)	1767
PagI	TCATGA	1 cut(s)	228
PceI	AGGCCT	1 cut(s)	344
PciI	ACATGT	2 cut(s)	750, 1356
PfeI	GAWTC	9 cut(s)	685, 711, 722, 1291, 1825, 1975, 2012, 2023, 2050
PkrI	GCNGC	2 cut(s)	844, 938
PleI	GAGTC	1 cut(s)	2008
PpsI	GAGTC	1 cut(s)	2008
Ppu21I	YACGTR	1 cut(s)	1391
PpuMI	RGGWCCY	1 cut(s)	308
PscI	ACATGT	2 cut(s)	750, 1356
PshBI	ATTAAT	1 cut(s)	1965
Psp124BI	GAGCTC	1 cut(s)	276
Psp5II	RGGWCCY	1 cut(s)	308
Psp6I	CCWGG	3 cut(s)	61, 1411, 1942
PspGI	CCWGG	3 cut(s)	61, 1411, 1942
PspN4I	GGNNCC	1 cut(s)	309
PspPI	GGNCC	2 cut(s)	308, 964
PspPPI	RGGWCCY	1 cut(s)	308
PsrI	GAACNNNNNNTAC	2 cut(s)	1679, 1711
PsuI	RGATCY	2 cut(s)	1420, 1660
PvuII	CAGCTG	1 cut(s)	487
RsaI	GTAC	2 cut(s)	1750, 2033
RsaNI	GTAC	2 cut(s)	1749, 2032
SacI	GAGCTC	1 cut(s)	276
SaqAI	TTAA	5 cut(s)	29, 1655, 1745, 1931, 1965
SatI	GCNGC	2 cut(s)	843, 937
Sau96I	GGNCC	2 cut(s)	308, 964
SchI	GAGTC	1 cut(s)	2009
ScrFI	CCNGG	3 cut(s)	63, 1413, 1944
SduI	GDGCHC	3 cut(s)	276, 1180, 1348
SexAI	ACCWGGT	1 cut(s)	1411
SfaNI	GCATC	2 cut(s)	48, 253
SfcI	CTRYAG	1 cut(s)	847
SfuI	TTCGAA	1 cut(s)	47
SinI	GGWCC	2 cut(s)	308, 964
SmlI	CTYRAG	5 cut(s)	28, 93, 269, 338, 2027
SmoI	CTYRAG	5 cut(s)	28, 93, 269, 338, 2027
SpeI	ACTAGT	2 cut(s)	911, 1238
SphI	GCATGC	1 cut(s)	1767
SseBI	AGGCCT	1 cut(s)	344
SsiI	CCGC	4 cut(s)	348, 1376, 1554, 1897
SspMI	CTAG	4 cut(s)	912, 951, 1181, 1239
SstI	GAGCTC	1 cut(s)	276
StuI	AGGCCT	1 cut(s)	344
StyD4I	CCNGG	3 cut(s)	61, 1411, 1942
TaaI	ACNGT	6 cut(s)	637, 866, 1040, 1234, 1303, 2067
TaiI	ACGT	2 cut(s)	1109, 1393
TaqI	TCGA	3 cut(s)	47, 709, 879
TaqII	GACCGA	1 cut(s)	952
TatI	WGTACW	1 cut(s)	1748
TfiI	GAWTC	9 cut(s)	685, 711, 722, 1291, 1825, 1975, 2012, 2023, 2050
Tru1I	TTAA	5 cut(s)	29, 1655, 1745, 1931, 1965
Tru9I	TTAA	5 cut(s)	29, 1655, 1745, 1931, 1965
TscAI	CASTG	3 cut(s)	1278, 1551, 2072
TseFI	GTSAC	1 cut(s)	860
TseI	GCWGC	2 cut(s)	842, 936
Tsp45I	GTSAC	1 cut(s)	860
TspGWI	ACGGA	1 cut(s)	1008
TspRI	CASTG	3 cut(s)	1278, 1551, 2072
Vha464I	CTTAAG	1 cut(s)	28
VpaK11BI	GGWCC	2 cut(s)	308, 964
VspI	ATTAAT	1 cut(s)	1965
XapI	RAATTY	3 cut(s)	220, 829, 983
XceI	RCATGY	6 cut(s)	39, 522, 754, 1360, 1560, 1767
XcmI	CCANNNNNNNNNTGG	1 cut(s)	974
XmiI	GTMKAC	1 cut(s)	747
XspI	CTAG	4 cut(s)	912, 951, 1181, 1239
ZraI	GACGTC	1 cut(s)	1107
Zsp2I	ATGCAT	1 cut(s)	41

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.