pycom03g21090

N-alpha-acetyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016172
Physical Location & Seq
Forward (+)
21413558 .. 21415636
2079 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 525 bp
ATGACGACGATACGCAGATTCCGCTGCAATGATCTTCTTCGATTCACCTCTGTGAATCTCGACCACCTCACCGAAACTTTCAATATGTCGTTTTACATGACGTATTTGGCGAGATGGCCCGACTACTTTCATGTTGCCGAAGCACCTGGACACCGAATTATGGGTTACATTATGGGAAAGGTTGAGGGGCAAGGTGAGTCATGGCATGGTCATGTAACTGCAGTAACTGTTTCTCCAGAATATCGCAGGCAGCAGTTGGCCAAGAAACTCATGAACTTGCTTGAAAACTTCAGCGATAAGATTGATAAGGGTTACTTTGTTGATCTTTTTGTGAGAGCATCAAACACACCCGCCATAAAGATGTACGAAAAGCTTGGCTATGTAATCTATAGACGAGTGCTACGGTACTATTCAGGGGAGGAAGACGGGTTAGATATGAGGAAGGCATTATCTCGTGACGTGGAGAAGAAATCAGTCATACCTCTCAAACGGCCAGTTACTCCCGACGAATTAGAGTATGACTAA

Protein Analysis

175

Amino Acids

20.51

Weight (kDa)

8.87

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 25 - 127 1.1e-17 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 41 - 128 2.3e-12 Acetyltransferase (GNAT) domain
Acetyltransf_10 PF13673 41 - 128 1.7e-10 Acetyltransferase (GNAT) domain
FR47 PF08445 70 - 131 1.7e-07 FR47-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03150
fragaria_vesca FvH4_2g23390
malus_domestica MD03G1263400.v1.1 MD11G1284200.v1.1
prunus_persica Prupe.8G239800_v2.0.a1
pyrus_communis pycom03g21090 pycom11g25160
rosa_chinensis RchiOBHm_Chr6g0290851
rosa_laevigata RLG00000012168
rosa_multiflora Rmu_sc0009276.1_g000006
rosa_roxburghii Rroxscaffold_7G00176690
rosa_rugosa Rorug06G0215200 Rorug06G0215300
rosa_samantha Rh6AG325800
rosa_wichuraiana Rw6G028330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 22, 351
AcoI YGGCCR 2 cut(s) 258, 491
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 2 cut(s) 365, 407
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 2 cut(s) 82, 284
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 1 cut(s) 145
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 1 cut(s) 373
AluI AGCT 1 cut(s) 373
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 3 cut(s) 116, 258, 491
ApeKI GCWGC 2 cut(s) 24, 250
AspS9I GGNCC 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 37, 61, 206
BalI TGGCCA 1 cut(s) 260
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 11, 262
BccI CCATC 1 cut(s) 108
BceAI ACGGC 1 cut(s) 506
BciT130I CCWGG 1 cut(s) 147
BfmI CTRYAG 2 cut(s) 219, 388
BisI GCNGC 2 cut(s) 25, 251
BlsI GCNGC 2 cut(s) 26, 252
Bme1390I CCNGG 1 cut(s) 147
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 429
BpmI CTGGAG 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 11, 262
BshFI GGCC 3 cut(s) 118, 260, 493
BslI CCNNNNNNNGG 1 cut(s) 160
BsnI GGCC 3 cut(s) 118, 260, 493
Bsp143I GATC 2 cut(s) 31, 322
BspACI CCGC 2 cut(s) 22, 351
BspANI GGCC 3 cut(s) 118, 260, 493
BspHI TCATGA 1 cut(s) 270
BspMAI CTGCAG 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 494
BssMI GATC 2 cut(s) 31, 322
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 229, 405
BstC8I GCNNGC 1 cut(s) 248
BstKTI GATC 2 cut(s) 34, 325
BstMBI GATC 2 cut(s) 31, 322
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 2 cut(s) 219, 388
BstV1I GCAGC 2 cut(s) 11, 262
BstV2I GAAGAC 1 cut(s) 429
BsuRI GGCC 3 cut(s) 118, 260, 493
BtrI CACGTC 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 248
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 117
Csp6I GTAC 2 cut(s) 364, 406
CviAII CATG 6 cut(s) 97, 131, 201, 206, 212, 271
CviJI RGCY 5 cut(s) 118, 260, 373, 378, 493
CviKI_1 RGCY 5 cut(s) 118, 260, 373, 378, 493
CviQI GTAC 2 cut(s) 364, 406
DpnI GATC 2 cut(s) 33, 324
DpnII GATC 2 cut(s) 31, 322
EaeI YGGCCR 2 cut(s) 258, 491
Eco57I CTGAAG 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 6 cut(s) 100, 134, 204, 209, 215, 274
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 6 cut(s) 96, 130, 200, 205, 211, 270
FauI CCCGC 1 cut(s) 358
Fnu4HI GCNGC 2 cut(s) 25, 251
Fsp4HI GCNGC 2 cut(s) 25, 251
GluI GCNGC 2 cut(s) 25, 251
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 3 cut(s) 118, 260, 493
Hin1II CATG 6 cut(s) 100, 134, 204, 209, 215, 274
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 4 cut(s) 18, 42, 55, 197
HphI GGTGA 3 cut(s) 37, 61, 206
Hpy188III TCNNGA 5 cut(s) 59, 236, 271, 455, 503
Hpy99I CGWCG 2 cut(s) 10, 509
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 2 cut(s) 229, 405
HpyCH4IV ACGT 2 cut(s) 101, 459
HpyCH4V TGCA 2 cut(s) 27, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpySE526I ACGT 2 cut(s) 101, 459
Hsp92II CATG 6 cut(s) 100, 134, 204, 209, 215, 274
Kzo9I GATC 2 cut(s) 31, 322
LpnPI CCDG 6 cut(s) 132, 159, 232, 249, 399, 507
Lsp1109I GCAGC 2 cut(s) 11, 262
LweI GCATC 1 cut(s) 347
MaeII ACGT 2 cut(s) 101, 459
MaeIII GTNAC 6 cut(s) 164, 214, 223, 311, 455, 496
MalI GATC 2 cut(s) 33, 324
MboI GATC 2 cut(s) 31, 322
MboII GAAGA 4 cut(s) 26, 29, 434, 478
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 2 cut(s) 156, 509
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 6 cut(s) 58, 77, 178, 412, 432, 492
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
MslI CAYNNNNRTG 3 cut(s) 50, 210, 359
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeII GATC 2 cut(s) 31, 322
NlaIII CATG 6 cut(s) 100, 134, 204, 209, 215, 274
NmuCI GTSAC 1 cut(s) 455
OliI CACNNNNGTG 1 cut(s) 50
PagI TCATGA 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PfeI GAWTC 3 cut(s) 18, 42, 55
PkrI GCNGC 2 cut(s) 26, 252
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspPI GGNCC 1 cut(s) 117
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 227
RsaI GTAC 2 cut(s) 365, 407
RsaNI GTAC 2 cut(s) 364, 406
RseI CAYNNNNRTG 3 cut(s) 50, 210, 359
SatI GCNGC 2 cut(s) 25, 251
Sau3AI GATC 2 cut(s) 31, 322
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 147
SetI ASST 9 cut(s) 50, 69, 104, 148, 183, 196, 375, 462, 484
SfaNI GCATC 1 cut(s) 347
SfcI CTRYAG 2 cut(s) 219, 388
SmiMI CAYNNNNRTG 3 cut(s) 50, 210, 359
Sse9I AATT 2 cut(s) 156, 509
SsiI CCGC 2 cut(s) 22, 351
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 229, 405
TaiI ACGT 2 cut(s) 104, 462
TaqI TCGA 2 cut(s) 40, 60
TasI AATT 2 cut(s) 156, 509
TfiI GAWTC 3 cut(s) 18, 42, 55
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 2 cut(s) 24, 250
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 119, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.