Rw6G028330

N-alpha-acetyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016172
Physical Location & Seq
Forward (+)
52289440 .. 52291508
2069 bp
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UTR
Exon/CDS
Intron
Rw6G028330.1

Sequence Viewer

Length: 411 bp
ATGGCCCGATTATTTTCATGTTGCGGAAGCCCGGGAAATCGAATCATGGGTTACATTATGGGAAAGGTTGAGGGGCAAGGTGAGTCGTGGCATGGTCATGTAACAGCGGTAACTGTTGCTCCAGAATATCGGAGGCAGCAGCTGGCCAAGAAGCTCATGAACCTCCTTGAAGACTTCAGCGATAAGATCGATAAGGGTTACTTTGTTGATCTTTTTGTGAGAGCATCAAACACTCCAGCCATAAAGATGTATGAGAAGCTTGGTTATATAATTTATAGACGAGTACTACGGTATTATTCAGGGGAGGAAGATGGGTTAGATATGAGGAAAGCATTATCTCGGGATGTTGAAAAGAAGTCAATCATACCCCTCAAACGACCAGTTACTCCTGATGAATTAGAGTATGACTAA

Protein Analysis

136

Amino Acids

15.67

Weight (kDa)

8.81

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 12 - 89 9.5e-17 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 13 - 90 5.1e-11 Acetyltransferase (GNAT) domain
Acetyltransf_10 PF13673 13 - 90 9.9e-10 Acetyltransferase (GNAT) domain
FR47 PF08445 32 - 94 6.1e-08 FR47-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03150
fragaria_vesca FvH4_2g23390
malus_domestica MD03G1263400.v1.1 MD11G1284200.v1.1
prunus_persica Prupe.8G239800_v2.0.a1
pyrus_communis pycom03g21090 pycom11g25160
rosa_chinensis RchiOBHm_Chr6g0290851
rosa_laevigata RLG00000012168
rosa_multiflora Rmu_sc0009276.1_g000006
rosa_roxburghii Rroxscaffold_7G00176690
rosa_rugosa Rorug06G0215200 Rorug06G0215300
rosa_samantha Rh6AG325800
rosa_wichuraiana Rw6G028330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 24, 107
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 160
AfaI GTAC 1 cut(s) 285
AgsI TTSAA 2 cut(s) 170, 350
AluBI AGCT 3 cut(s) 142, 154, 259
AluI AGCT 3 cut(s) 142, 154, 259
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 2 cut(s) 31, 339
AoxI GGCC 2 cut(s) 3, 144
ApeKI GCWGC 2 cut(s) 136, 139
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 2 cut(s) 32, 33
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 2 cut(s) 31, 339
BalI TGGCCA 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 148, 151
BccI CCATC 1 cut(s) 305
BcnI CCSGG 2 cut(s) 32, 33
BisI GCNGC 2 cut(s) 137, 140
BlsI GCNGC 2 cut(s) 138, 141
BmcAI AGTACT 1 cut(s) 285
Bme1390I CCNGG 2 cut(s) 32, 33
BmeT110I CYCGRG 2 cut(s) 31, 339
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 2 cut(s) 32, 33
BmsI GCATC 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 2 cut(s) 105, 219
BpuMI CCSGG 2 cut(s) 32, 33
Bsa29I ATCGAT 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 103, 133
Bse1I ACTGG 1 cut(s) 380
BseCI ATCGAT 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 349
BseNI ACTGG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 148, 151
BshFI GGCC 2 cut(s) 5, 146
BshVI ATCGAT 1 cut(s) 189
BsiHKCI CYCGRG 2 cut(s) 31, 339
BsiSI CCGG 1 cut(s) 32
BsnI GGCC 2 cut(s) 5, 146
BsoBI CYCGRG 2 cut(s) 31, 339
Bsp143I GATC 2 cut(s) 186, 208
BspACI CCGC 2 cut(s) 24, 107
BspANI GGCC 2 cut(s) 5, 146
BspDI ATCGAT 1 cut(s) 189
BspHI TCATGA 1 cut(s) 156
BsrI ACTGG 1 cut(s) 380
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 2 cut(s) 186, 208
Bst4CI ACNGT 2 cut(s) 115, 291
BstC8I GCNNGC 1 cut(s) 144
BstF5I GGATG 1 cut(s) 349
BstKTI GATC 2 cut(s) 189, 211
BstMBI GATC 2 cut(s) 186, 208
BstSCI CCNGG 2 cut(s) 30, 31
BstV1I GCAGC 2 cut(s) 148, 151
BstV2I GAAGAC 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 189
BsuRI GGCC 2 cut(s) 5, 146
BsuTUI ATCGAT 1 cut(s) 189
BtsCI GGATG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 4
Cfr9I CCCGGG 1 cut(s) 31
ClaI ATCGAT 1 cut(s) 189
Csp6I GTAC 1 cut(s) 284
CviAII CATG 5 cut(s) 18, 46, 92, 98, 157
CviJI RGCY 7 cut(s) 5, 30, 142, 146, 154, 239, 259
CviKI_1 RGCY 7 cut(s) 5, 30, 142, 146, 154, 239, 259
CviQI GTAC 1 cut(s) 284
DpnI GATC 2 cut(s) 188, 210
DpnII GATC 2 cut(s) 186, 208
EaeI YGGCCR 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 160
Eco88I CYCGRG 2 cut(s) 31, 339
FaeI CATG 5 cut(s) 21, 49, 95, 101, 160
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 5 cut(s) 17, 45, 91, 97, 156
Fnu4HI GCNGC 2 cut(s) 137, 140
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 2 cut(s) 137, 140
GluI GCNGC 2 cut(s) 137, 140
GsuI CTGGAG 2 cut(s) 105, 219
HaeIII GGCC 2 cut(s) 5, 146
HapII CCGG 1 cut(s) 32
Hin1II CATG 5 cut(s) 21, 49, 95, 101, 160
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 2 cut(s) 42, 83
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 4 cut(s) 122, 157, 341, 389
HpyCH4III ACNGT 2 cut(s) 115, 291
Hsp92II CATG 5 cut(s) 21, 49, 95, 101, 160
Kzo9I GATC 2 cut(s) 186, 208
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 7 cut(s) 45, 128, 135, 249, 285, 393, 402
Lsp1109I GCAGC 2 cut(s) 148, 151
LweI GCATC 1 cut(s) 233
MaeIII GTNAC 5 cut(s) 50, 100, 109, 197, 382
MalI GATC 2 cut(s) 188, 210
MboI GATC 2 cut(s) 186, 208
MboII GAAGA 2 cut(s) 182, 320
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 2 cut(s) 270, 395
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 6 cut(s) 64, 126, 173, 298, 318, 380
Mox20I TGGCCA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 146
MslI CAYNNNNRTG 2 cut(s) 96, 245
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 2 cut(s) 107, 142
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 32, 33
NciI CCSGG 2 cut(s) 32, 33
NdeII GATC 2 cut(s) 186, 208
NlaIII CATG 5 cut(s) 21, 49, 95, 101, 160
PagI TCATGA 1 cut(s) 156
PfeI GAWTC 1 cut(s) 42
PkrI GCNGC 2 cut(s) 138, 141
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 2 cut(s) 96, 245
SatI GCNGC 2 cut(s) 137, 140
Sau3AI GATC 2 cut(s) 186, 208
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 285
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 32, 33
SetI ASST 6 cut(s) 69, 82, 144, 156, 165, 261
SfaNI GCATC 1 cut(s) 233
SmaI CCCGGG 1 cut(s) 33
SmiMI CAYNNNNRTG 2 cut(s) 96, 245
Sse9I AATT 2 cut(s) 270, 395
SsiI CCGC 2 cut(s) 24, 107
StyD4I CCNGG 2 cut(s) 30, 31
TaaI ACNGT 2 cut(s) 115, 291
TaqI TCGA 2 cut(s) 40, 189
TasI AATT 2 cut(s) 270, 395
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 1 cut(s) 42
TseI GCWGC 2 cut(s) 136, 139
TspDTI ATGAA 3 cut(s) 6, 173, 408
TspMI CCCGGG 1 cut(s) 31
XmaI CCCGGG 1 cut(s) 31
ZrmI AGTACT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.