RLG00000012168

N-alpha-acetyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016172
Physical Location & Seq
Reverse (-)
15948960 .. 15951064
2105 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012168

Sequence Viewer

Length: 525 bp
ATGACTACGATTCGGAGATTTTGCTGCAATGATCTGCTTCGTTTCACTTCGGTCAATCTTGACCACCTCACTGAAACATTCAATATGTCGTTCTATATGACCTATTTGGCGAGATGGCCCGACTATTTTCACGTTGCCGAAGCCCCGGGAAATCGAATCATGGGTTACATTATGGGAAAGGTTGAGGGGCAAGGTGAGTCGTGGCATGGTCATGTAACTGCAGTAACTGTTGCTCCAGAATATCGGAGGCAGCAGCTGGCCAAGAAGCTCATGAACCTCCTTGAAAACTTCAGCGATAAGATTGATAAGGGTTACTTTGTTGATCTTTTTGTGAGAGCATCAAACACTCCAGCCATAAAGATGTATGAGAAGCTTGGTTATATAATTTATAGACGAGTACTACGGTATTATTCAGGGGAGGAAGATGGGTTAGATATGAGGAAAGCATTATCTCGGGATGTTGAAAAGAAATCAATCATACCCCTCAAACGGCCAGTTACTCCTGATGAATTAGAGTATGACTAA

Protein Analysis

175

Amino Acids

20.44

Weight (kDa)

8.44

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 26 - 127 6.6e-18 Acetyltransferase (GNAT) family
Acetyltransf_10 PF13673 40 - 128 1.2e-10 Acetyltransferase (GNAT) domain
Acetyltransf_7 PF13508 41 - 128 1.8e-12 Acetyltransferase (GNAT) domain
FR47 PF08445 70 - 131 9.7e-08 FR47-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03150
fragaria_vesca FvH4_2g23390
malus_domestica MD03G1263400.v1.1 MD11G1284200.v1.1
prunus_persica Prupe.8G239800_v2.0.a1
pyrus_communis pycom03g21090 pycom11g25160
rosa_chinensis RchiOBHm_Chr6g0290851
rosa_laevigata RLG00000012168
rosa_multiflora Rmu_sc0009276.1_g000006
rosa_roxburghii Rroxscaffold_7G00176690
rosa_rugosa Rorug06G0215200 Rorug06G0215300
rosa_samantha Rh6AG325800
rosa_wichuraiana Rw6G028330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 258, 491
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 1 cut(s) 489
AgsI TTSAA 3 cut(s) 82, 284, 464
AluBI AGCT 3 cut(s) 256, 268, 373
AluI AGCT 3 cut(s) 256, 268, 373
AlwNI CAGNNNCTG 2 cut(s) 227, 256
Ama87I CYCGRG 2 cut(s) 145, 453
AoxI GGCC 3 cut(s) 116, 258, 491
ApeKI GCWGC 3 cut(s) 24, 250, 253
AspS9I GGNCC 1 cut(s) 117
AsuC2I CCSGG 2 cut(s) 146, 147
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 2 cut(s) 145, 453
BalI TGGCCA 1 cut(s) 260
BbvI GCAGC 3 cut(s) 11, 262, 265
BccI CCATC 2 cut(s) 108, 419
BceAI ACGGC 1 cut(s) 506
BcnI CCSGG 2 cut(s) 146, 147
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 25, 251, 254
BlsI GCNGC 3 cut(s) 26, 252, 255
BmcAI AGTACT 1 cut(s) 399
Bme1390I CCNGG 2 cut(s) 146, 147
BmeT110I CYCGRG 2 cut(s) 145, 453
BmgT120I GGNCC 1 cut(s) 117
BmrFI CCNGG 2 cut(s) 146, 147
BmsI GCATC 1 cut(s) 347
BpmI CTGGAG 2 cut(s) 219, 333
BpuMI CCSGG 2 cut(s) 146, 147
BsaJI CCNNGG 2 cut(s) 144, 145
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 34
BseDI CCNNGG 2 cut(s) 144, 145
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 494
BseXI GCAGC 3 cut(s) 11, 262, 265
BshFI GGCC 3 cut(s) 118, 260, 493
BsiHKCI CYCGRG 2 cut(s) 145, 453
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 489
BsnI GGCC 3 cut(s) 118, 260, 493
BsoBI CYCGRG 2 cut(s) 145, 453
Bsp143I GATC 2 cut(s) 31, 322
BspANI GGCC 3 cut(s) 118, 260, 493
BspHI TCATGA 1 cut(s) 270
BspMAI CTGCAG 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 494
BssECI CCNNGG 2 cut(s) 144, 145
BssMI GATC 2 cut(s) 31, 322
Bst4CI ACNGT 2 cut(s) 229, 405
BstC8I GCNNGC 1 cut(s) 258
BstF5I GGATG 1 cut(s) 463
BstKTI GATC 2 cut(s) 34, 325
BstMBI GATC 2 cut(s) 31, 322
BstSCI CCNGG 2 cut(s) 144, 145
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 11, 262, 265
BsuRI GGCC 3 cut(s) 118, 260, 493
BtsCI GGATG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 258
CaiI CAGNNNCTG 2 cut(s) 227, 256
CciI TCATGA 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 117
Cfr9I CCCGGG 1 cut(s) 145
Csp6I GTAC 1 cut(s) 398
CviAII CATG 4 cut(s) 160, 206, 212, 271
CviJI RGCY 8 cut(s) 118, 143, 256, 260, 268, 353, 373, 493
CviKI_1 RGCY 8 cut(s) 118, 143, 256, 260, 268, 353, 373, 493
CviQI GTAC 1 cut(s) 398
DpnI GATC 2 cut(s) 33, 324
DpnII GATC 2 cut(s) 31, 322
EaeI YGGCCR 2 cut(s) 258, 491
Eco57I CTGAAG 1 cut(s) 274
Eco88I CYCGRG 2 cut(s) 145, 453
FaeI CATG 4 cut(s) 163, 209, 215, 274
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 4 cut(s) 159, 205, 211, 270
Fnu4HI GCNGC 3 cut(s) 25, 251, 254
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 3 cut(s) 25, 251, 254
GluI GCNGC 3 cut(s) 25, 251, 254
GsuI CTGGAG 2 cut(s) 219, 333
HaeIII GGCC 3 cut(s) 118, 260, 493
HapII CCGG 1 cut(s) 146
Hin1II CATG 4 cut(s) 163, 209, 215, 274
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 3 cut(s) 10, 156, 197
HpaII CCGG 1 cut(s) 146
HphI GGTGA 1 cut(s) 206
Hpy188I TCNGA 2 cut(s) 15, 246
Hpy188III TCNNGA 5 cut(s) 59, 236, 271, 455, 503
HpyCH4III ACNGT 2 cut(s) 229, 405
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 27, 221
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 4 cut(s) 163, 209, 215, 274
Kzo9I GATC 2 cut(s) 31, 322
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 7 cut(s) 159, 242, 249, 363, 399, 507, 516
Lsp1109I GCAGC 3 cut(s) 11, 262, 265
LweI GCATC 1 cut(s) 347
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 5 cut(s) 164, 214, 223, 311, 496
MalI GATC 2 cut(s) 33, 324
MboI GATC 2 cut(s) 31, 322
MboII GAAGA 1 cut(s) 434
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 2 cut(s) 384, 509
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 77, 178, 240, 287, 412, 432, 494
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
MslI CAYNNNNRTG 2 cut(s) 210, 359
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 146, 147
NciI CCSGG 2 cut(s) 146, 147
NdeII GATC 2 cut(s) 31, 322
NlaIII CATG 4 cut(s) 163, 209, 215, 274
PagI TCATGA 1 cut(s) 270
PfeI GAWTC 2 cut(s) 10, 156
PkrI GCNGC 3 cut(s) 26, 252, 255
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 117
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 2 cut(s) 227, 256
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 2 cut(s) 210, 359
SatI GCNGC 3 cut(s) 25, 251, 254
Sau3AI GATC 2 cut(s) 31, 322
Sau96I GGNCC 1 cut(s) 117
ScaI AGTACT 1 cut(s) 399
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 146, 147
SetI ASST 9 cut(s) 69, 104, 135, 183, 196, 258, 270, 279, 375
SfaNI GCATC 1 cut(s) 347
SfcI CTRYAG 1 cut(s) 219
SmaI CCCGGG 1 cut(s) 147
SmiMI CAYNNNNRTG 2 cut(s) 210, 359
Sse9I AATT 2 cut(s) 384, 509
StyD4I CCNGG 2 cut(s) 144, 145
TaaI ACNGT 2 cut(s) 229, 405
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 154
TaqII GACCGA 1 cut(s) 40
TasI AATT 2 cut(s) 384, 509
TatI WGTACW 1 cut(s) 397
TfiI GAWTC 2 cut(s) 10, 156
TscAI CASTG 1 cut(s) 76
TseI GCWGC 3 cut(s) 24, 250, 253
TspDTI ATGAA 2 cut(s) 287, 522
TspMI CCCGGG 1 cut(s) 145
TspRI CASTG 1 cut(s) 76
XmaI CCCGGG 1 cut(s) 145
ZrmI AGTACT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.