pycom03g23200

PAPA-1-like conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012739
Physical Location & Seq
Forward (+)
23113903 .. 23116456
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23200.3

Sequence Viewer

Length: 1758 bp
ATGGAAGACTTGGGCAACAATCATAGATTGGACAGTATAAGCAGTGCTGTAAGAAAGAAGAGAAGTCAAACGTTTCGTCGACCTCGACCTGATGCATATACTGAACTCCATGATGAGTCACCGTTGTCATCAACTACACCTTCGGATGATCAGAGCAAGGTCTCTAGTGATGAGAATGCAGGTTGTGATGCCAATTCAAAGAGAAAAGAATTAAGTCTTAATGAATGTATGGCCAGGGGTTCTTCTGCGGCAGGTAATGGCGAAAAACCTCACAAAAAGGATAGTAAGGGTGGAGGATGTAATTCGTTTTACAAGAATGAGCAAGGACGGAATGAGAGCAATAACAAGCGTTCTAGTGAAGGTTGCCTTGCCCCTGCTAATTGGAAGAGCTCCAGCTTAATGAAGGATGATTTGATATCGGAGTCAATAAGTGCTGGTGCATTTAATGGGAGGAATGGTGAAAGTCCAAGTACTAGGTTGTCAGGGTTTGATGGATTTGGAAATGAGAACAAGGTTAAAAAGGTTAAGCTCAAGGTTGGCGGTGTCACACGTACAATTCAGGCCAACTCTACATTGAATGGTACAACGGAGGGTGGGTCTACCACAAAGACTGCTCGATTGTCAGATGCGTCTAGACCACGGCAAAAGCAAAATCTTCTGGAAAATTCGGATGATAATCATTCTCCTTTGGATAAGAAGCGAGGGTTAAAAGGAATTCCATGGAAGGATTTCTCAAGAAGTGGTATTAGTCTCGGGAGGGATAATTGTTCGATGGGCAGGACAGCGGGAAAGAATACCTATGGTAAGGAAGGCGATGGATCTGAACCAGTTCGTAAGAGCAAGCGAGTACCTAAGAGGCGCATTCTTGATGGAGATGTTGGAGATGAAGAAGAGGATGATGAGATTCGGTATCTGGAGAAACTCAAAATATCAAAGGTTGCTGCAGTTTATAGAGACGACGATGATGAATCAAGCAGGAAGCACAGAAAACTTTCTGCAGTTTCCAACATTGATAATGCCAGTGCATCAAGGTTGGATAAAGACCTTAAAAGGAAGTCGAGAACTGATAGAGTATCTGGCGACACTGATTATGAGGAAGAACAAGGTTCATTATCTGATGGCGAGCTTGAATGTAAAAAGAAACAGAAGAAAGAAGCTGTTGACTCTTTGGTGGATGGTAAGAAGGAAGTGACTCTCACAACTCGTCAAAGGACCCTTCAGTCAAGCAAAGATGCCACCCCTGGTTCATGCTTAATTGAGTTTCCAAATGGATTACCACCTGCACCATCTAGAAAGCAAAAGGAAAAACTTACAGATGTGGAGCAGCAACTGAAGAAAGCTGAGGCTGCTCAGAGACGAAGGATGCAGGTTGAGAAGGCTGCTAGGGAATCAGAGGCTGAGGCTATTAGAAAAATTCTCGGTCAAGATTCCAGCAGGAAGAAGCGTGAAGACAAGATAAAGAAGCGACAAGAAGAAATTGCACAGGAGAGGGCTGCTAATGCCTTGACACTTCCGCCAAACACCATCAGAACAGTGATGGGCCCAGCGGGTACTGTAGTTACATTCTCCAATGATATGGGTCTCCCCAGTTTATTTGACCCAAAACCCTGCAGCTATCCAACTCAGCGTGAGAATTGCGCAGGTCCCTTGTGTACCAATCCATACAAGTATAGGGATTCTAAGTCACAGCTTCCTCTTTGCAGTCTTAAGTGCTACAAGGCAATCCAGGAAAAGACAGCGGTGGGAACTACATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAPA-1 zf-HIT

Protein Analysis

586

Amino Acids

64.45

Weight (kDa)

9.3

Isoelectric Point (pI)

51.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012739)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47350 AT2G47350
fragaria_vesca FvH4_5g39030
malus_domestica MD00G1140600.v1.1 MD03G1292700.v1.1
prunus_persica Prupe.8G265700_v2.0.a1
pyrus_communis pycom03g23200 pycom11g27340
rosa_chinensis RchiOBHm_Chr7g0241001
rosa_laevigata RLG00000000605
rosa_multiflora Rmu_sc0003187.1_g000010
rosa_roxburghii Rroxscaffold_3G00219330
rosa_rugosa Rorug07G0341200
rosa_samantha Rh7AG496300 Rh7BG467600 Rh7CG513600 Rh7DG480800
rosa_wichuraiana Rw7G041920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1288
AasI GACNNNNNNGTC 1 cut(s) 1219
Acc16I TGCGCA 1 cut(s) 1639
Acc36I ACCTGC 5 cut(s) 170, 242, 1288, 1357, 1631
AccI GTMKAC 2 cut(s) 79, 599
AciI CCGC 6 cut(s) 248, 540, 785, 1514, 1547, 1739
AclI AACGTT 1 cut(s) 71
AclWI GGATC 1 cut(s) 826
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 3 cut(s) 664, 714, 1413
AcuI CTGAAG 2 cut(s) 1202, 1352
AfaI GTAC 6 cut(s) 472, 553, 583, 849, 1552, 1654
AflII CTTAAG 1 cut(s) 1706
AflIII ACRYGT 1 cut(s) 548
AgsI TTSAA 3 cut(s) 198, 577, 1130
AjnI CCWGG 3 cut(s) 233, 1240, 1725
AluBI AGCT 8 cut(s) 390, 396, 529, 1126, 1157, 1340, 1614, 1690
AluI AGCT 8 cut(s) 390, 396, 529, 1126, 1157, 1340, 1614, 1690
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 5 cut(s) 166, 755, 948, 1348, 1586
AlwI GGATC 1 cut(s) 826
AlwNI CAGNNNCTG 2 cut(s) 1330, 1397
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 3 cut(s) 231, 561, 1540
ApaI GGGCCC 1 cut(s) 1544
ApeKI GCWGC 6 cut(s) 941, 1324, 1346, 1379, 1493, 1611
ApoI RAATTY 3 cut(s) 664, 714, 1413
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
Asp700I GAANNNNTTC 3 cut(s) 728, 828, 991
AspLEI GCGC 2 cut(s) 861, 1640
AspS9I GGNCC 4 cut(s) 1212, 1540, 1541, 1643
AsuHPI GGTGA 2 cut(s) 111, 470
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 2 cut(s) 1212, 1643
BaeGI GKGCMC 1 cut(s) 1544
BaeI ACNNNNGTAYC 2 cut(s) 1542, 1575
BalI TGGCCA 1 cut(s) 233
BanII GRGCYC 2 cut(s) 392, 1544
BbsI GAAGAC 2 cut(s) 12, 1455
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 2 cut(s) 1341, 1398
BbvI GCAGC 6 cut(s) 928, 1333, 1336, 1366, 1480, 1623
BccI CCATC 9 cut(s) 485, 766, 809, 863, 1112, 1169, 1294, 1531, 1532
BceAI ACGGC 1 cut(s) 656
BciT130I CCWGG 3 cut(s) 235, 1242, 1727
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 5 cut(s) 166, 755, 948, 1348, 1586
BfaI CTAG 6 cut(s) 165, 354, 474, 633, 1290, 1383
BfmI CTRYAG 4 cut(s) 942, 996, 1554, 1609
BfrI CTTAAG 1 cut(s) 1706
BfuAI ACCTGC 5 cut(s) 170, 242, 1288, 1357, 1631
BisI GCNGC 7 cut(s) 249, 942, 1325, 1347, 1380, 1494, 1612
BlsI GCNGC 7 cut(s) 250, 943, 1326, 1348, 1381, 1495, 1613
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 3 cut(s) 235, 1242, 1727
Bme18I GGWCC 2 cut(s) 1212, 1643
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 4 cut(s) 1212, 1540, 1541, 1643
BmiI GGNNCC 3 cut(s) 1214, 1542, 1645
BmrFI CCNGG 3 cut(s) 235, 1242, 1727
BmrI ACTGGG 1 cut(s) 1581
BmsI GCATC 6 cut(s) 82, 178, 616, 1034, 1222, 1353
BmuI ACTGGG 1 cut(s) 1581
BpiI GAAGAC 2 cut(s) 12, 1455
BpmI CTGGAG 2 cut(s) 376, 935
Bpu10I CCTNAGC 2 cut(s) 1341, 1398
BpuEI CTTGAG 2 cut(s) 515, 718
BsaAI YACGTR 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 675
BsaI GGTCTC 2 cut(s) 166, 1586
BsaJI CCNNGG 4 cut(s) 234, 638, 719, 1240
BsaXI ACNNNNNCTCC 4 cut(s) 442, 472, 581, 611
Bse1I ACTGG 3 cut(s) 827, 1020, 1587
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 3 cut(s) 235, 1242, 1727
BseDI CCNNGG 4 cut(s) 234, 638, 719, 1240
BseGI GGATG 7 cut(s) 151, 302, 412, 676, 901, 1180, 1368
BseJI GATNNNNATC 1 cut(s) 675
BseMII CTCAG 4 cut(s) 1332, 1364, 1389, 1637
BseNI ACTGG 3 cut(s) 827, 1020, 1587
BseSI GKGCMC 1 cut(s) 1544
BseXI GCAGC 6 cut(s) 928, 1333, 1336, 1366, 1480, 1623
BseYI CCCAGC 1 cut(s) 1543
BsgI GTGCAG 1 cut(s) 1266
BshFI GGCC 3 cut(s) 233, 563, 1542
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 752
BslFI GGGAC 1 cut(s) 1629
BsmAI GTCTC 5 cut(s) 166, 755, 948, 1348, 1586
BsmBI CGTCTC 2 cut(s) 948, 1348
BsmFI GGGAC 1 cut(s) 1629
BsmI GAATGC 2 cut(s) 181, 861
BsnI GGCC 3 cut(s) 233, 563, 1542
Bso31I GGTCTC 2 cut(s) 166, 1586
BsoBI CYCGRG 1 cut(s) 752
Bsp120I GGGCCC 1 cut(s) 1540
Bsp1286I GDGCHC 2 cut(s) 392, 1544
Bsp143I GATC 2 cut(s) 148, 818
Bsp19I CCATGG 1 cut(s) 719
BspACI CCGC 6 cut(s) 248, 540, 785, 1514, 1547, 1739
BspANI GGCC 3 cut(s) 233, 563, 1542
BspCNI CTCAG 4 cut(s) 1333, 1363, 1390, 1636
BspLI GGNNCC 3 cut(s) 1214, 1542, 1645
BspMAI CTGCAG 3 cut(s) 946, 1000, 1613
BspMI ACCTGC 5 cut(s) 170, 242, 1288, 1357, 1631
BspPI GGATC 1 cut(s) 826
BspQI GCTCTTC 1 cut(s) 380
BspTI CTTAAG 1 cut(s) 1706
BspTNI GGTCTC 2 cut(s) 166, 1586
BsrI ACTGG 3 cut(s) 827, 1020, 1587
BssECI CCNNGG 4 cut(s) 234, 638, 719, 1240
BssMI GATC 2 cut(s) 148, 818
BssT1I CCWWGG 1 cut(s) 719
Bst2UI CCWGG 3 cut(s) 235, 1242, 1727
Bst4CI ACNGT 4 cut(s) 35, 123, 1534, 1555
Bst6I CTCTTC 3 cut(s) 53, 380, 885
BstAFI CTTAAG 1 cut(s) 1706
BstBAI YACGTR 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 842, 1124
BstDEI CTNAG 6 cut(s) 852, 1341, 1350, 1398, 1623, 1680
BstDSI CCRYGG 2 cut(s) 638, 719
BstF5I GGATG 7 cut(s) 151, 302, 412, 676, 901, 1180, 1368
BstHHI GCGC 2 cut(s) 861, 1640
BstKTI GATC 2 cut(s) 151, 821
BstMAI GTCTC 5 cut(s) 166, 755, 948, 1348, 1586
BstMBI GATC 2 cut(s) 148, 818
BstMWI GCNNNNNNNGC 2 cut(s) 1346, 1499
BstNI CCWGG 3 cut(s) 235, 1242, 1727
BstNSI RCATGY 1 cut(s) 1755
BstSCI CCNGG 3 cut(s) 233, 1240, 1725
BstSFI CTRYAG 4 cut(s) 942, 996, 1554, 1609
BstSLI GKGCMC 1 cut(s) 1544
BstV1I GCAGC 6 cut(s) 928, 1333, 1336, 1366, 1480, 1623
BstV2I GAAGAC 2 cut(s) 12, 1455
BstX2I RGATCY 1 cut(s) 818
BstXI CCANNNNNNTGG 1 cut(s) 1576
BstYI RGATCY 1 cut(s) 818
BsuRI GGCC 3 cut(s) 233, 563, 1542
BtgI CCRYGG 2 cut(s) 638, 719
BtgZI GCGATG 1 cut(s) 828
BtsCI GGATG 7 cut(s) 151, 302, 412, 676, 901, 1180, 1368
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 4 cut(s) 49, 1027, 1083, 1539
BveI ACCTGC 5 cut(s) 170, 242, 1288, 1357, 1631
Cac8I GCNNGC 2 cut(s) 842, 1124
CaiI CAGNNNCTG 2 cut(s) 1330, 1397
CfoI GCGC 2 cut(s) 861, 1640
Cfr13I GGNCC 4 cut(s) 1212, 1540, 1541, 1643
CseI GACGC 1 cut(s) 618
Csp6I GTAC 6 cut(s) 471, 552, 582, 848, 1551, 1653
CviAII CATG 4 cut(s) 110, 720, 1248, 1752
CviQI GTAC 6 cut(s) 471, 552, 582, 848, 1551, 1653
DdeI CTNAG 6 cut(s) 852, 1341, 1350, 1398, 1623, 1680
DpnI GATC 2 cut(s) 150, 820
DpnII GATC 2 cut(s) 148, 818
DrdI GACNNNNNNGTC 1 cut(s) 1219
DseDI GACNNNNNNGTC 1 cut(s) 1219
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 3 cut(s) 53, 380, 885
EarI CTCTTC 3 cut(s) 53, 380, 885
EciI GGCGGA 1 cut(s) 1503
Ecl136II GAGCTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 719
Eco24I GRGCYC 2 cut(s) 392, 1544
Eco31I GGTCTC 2 cut(s) 166, 1586
Eco32I GATATC 1 cut(s) 417
Eco47I GGWCC 2 cut(s) 1212, 1643
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 2 cut(s) 1202, 1352
Eco88I CYCGRG 1 cut(s) 752
EcoICRI GAGCTC 1 cut(s) 390
EcoO109I RGGNCCY 2 cut(s) 1212, 1643
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 3 cut(s) 233, 1240, 1725
EcoRV GATATC 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 719
EcoT22I ATGCAT 1 cut(s) 97
EcoT38I GRGCYC 2 cut(s) 392, 1544
ErhI CCWWGG 1 cut(s) 719
Esp3I CGTCTC 2 cut(s) 948, 1348
FaeI CATG 4 cut(s) 113, 723, 1251, 1755
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 1 cut(s) 1629
FatI CATG 4 cut(s) 109, 719, 1247, 1751
FauI CCCGC 2 cut(s) 778, 1540
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 2 cut(s) 79, 599
Fnu4HI GCNGC 7 cut(s) 249, 942, 1325, 1347, 1380, 1494, 1612
FokI GGATG 7 cut(s) 158, 309, 419, 683, 908, 1187, 1375
FriOI GRGCYC 2 cut(s) 392, 1544
Fsp4HI GCNGC 7 cut(s) 249, 942, 1325, 1347, 1380, 1494, 1612
FspBI CTAG 6 cut(s) 165, 354, 474, 633, 1290, 1383
FspI TGCGCA 1 cut(s) 1639
GlaI GCGC 2 cut(s) 860, 1639
GluI GCNGC 7 cut(s) 249, 942, 1325, 1347, 1380, 1494, 1612
GsaI CCCAGC 1 cut(s) 1547
GsuI CTGGAG 2 cut(s) 376, 935
HaeIII GGCC 3 cut(s) 233, 563, 1542
HgaI GACGC 1 cut(s) 618
HhaI GCGC 2 cut(s) 861, 1640
Hin1II CATG 4 cut(s) 113, 723, 1251, 1755
Hin6I GCGC 2 cut(s) 859, 1638
HinP1I GCGC 2 cut(s) 859, 1638
HincII GTYRAC 2 cut(s) 80, 1162
HindII GTYRAC 2 cut(s) 80, 1162
HinfI GANTC 9 cut(s) 116, 422, 904, 968, 1163, 1192, 1388, 1427, 1676
HphI GGTGA 2 cut(s) 111, 470
Hpy166II GTNNAC 4 cut(s) 80, 600, 1162, 1653
Hpy188III TCNNGA 9 cut(s) 633, 659, 735, 754, 866, 914, 1059, 1290, 1424
Hpy8I GTNNAC 4 cut(s) 80, 600, 1162, 1653
Hpy99I CGWCG 2 cut(s) 81, 962
HpyAV CCTTC 9 cut(s) 150, 353, 397, 718, 803, 1177, 1226, 1353, 1369
HpyCH4III ACNGT 4 cut(s) 35, 123, 1534, 1555
HpyCH4IV ACGT 2 cut(s) 71, 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 1346, 1499
HpyF3I CTNAG 6 cut(s) 852, 1341, 1350, 1398, 1623, 1680
HpySE526I ACGT 2 cut(s) 71, 550
Hsp92II CATG 4 cut(s) 113, 723, 1251, 1755
HspAI GCGC 2 cut(s) 859, 1638
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 2 cut(s) 148, 818
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 2 cut(s) 395, 1321
Lsp1109I GCAGC 6 cut(s) 928, 1333, 1336, 1366, 1480, 1623
LweI GCATC 6 cut(s) 82, 178, 616, 1034, 1222, 1353
MaeI CTAG 6 cut(s) 165, 354, 474, 633, 1290, 1383
MaeII ACGT 2 cut(s) 71, 550
MaeIII GTNAC 5 cut(s) 117, 544, 1189, 1558, 1683
MalI GATC 2 cut(s) 150, 820
MboI GATC 2 cut(s) 148, 818
MflI RGATCY 1 cut(s) 818
MhlI GDGCHC 2 cut(s) 392, 1544
MlsI TGGCCA 1 cut(s) 233
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 4 cut(s) 125, 431, 1157, 1186
MmeI TCCRAC 4 cut(s) 859, 1014, 1029, 1643
Mox20I TGGCCA 1 cut(s) 233
Mph1103I ATGCAT 1 cut(s) 97
MroXI GAANNNNTTC 3 cut(s) 728, 828, 991
MscI TGGCCA 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 3 cut(s) 785, 1547, 1739
MspCI CTTAAG 1 cut(s) 1706
MspR9I CCNGG 3 cut(s) 235, 1242, 1727
Mva1269I GAATGC 2 cut(s) 181, 861
MvaI CCWGG 3 cut(s) 235, 1242, 1727
MwoI GCNNNNNNNGC 2 cut(s) 1346, 1499
NcoI CCATGG 1 cut(s) 719
NdeII GATC 2 cut(s) 148, 818
NlaIII CATG 4 cut(s) 113, 723, 1251, 1755
NlaIV GGNNCC 3 cut(s) 1214, 1542, 1645
NmuCI GTSAC 4 cut(s) 117, 544, 1189, 1683
NsbI TGCGCA 1 cut(s) 1639
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 1 cut(s) 1755
PaqCI CACCTGC 1 cut(s) 1288
PciSI GCTCTTC 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 82
PctI GAATGC 2 cut(s) 181, 861
PdmI GAANNNNTTC 3 cut(s) 728, 828, 991
PfeI GAWTC 5 cut(s) 904, 968, 1388, 1427, 1676
PfoI TCCNGGA 1 cut(s) 1725
PkrI GCNGC 7 cut(s) 250, 943, 1326, 1348, 1381, 1495, 1613
PleI GAGTC 4 cut(s) 124, 430, 1157, 1186
PpsI GAGTC 4 cut(s) 124, 430, 1157, 1186
Ppu21I YACGTR 1 cut(s) 551
PpuMI RGGWCCY 2 cut(s) 1212, 1643
Psp124BI GAGCTC 1 cut(s) 392
Psp1406I AACGTT 1 cut(s) 71
Psp5II RGGWCCY 2 cut(s) 1212, 1643
Psp6I CCWGG 3 cut(s) 233, 1240, 1725
PspFI CCCAGC 1 cut(s) 1543
PspGI CCWGG 3 cut(s) 233, 1240, 1725
PspN4I GGNNCC 3 cut(s) 1214, 1542, 1645
PspOMI GGGCCC 1 cut(s) 1540
PspPI GGNCC 4 cut(s) 1212, 1540, 1541, 1643
PspPPI RGGWCCY 2 cut(s) 1212, 1643
PstI CTGCAG 3 cut(s) 946, 1000, 1613
PstNI CAGNNNCTG 2 cut(s) 1330, 1397
PsuI RGATCY 1 cut(s) 818
RsaI GTAC 6 cut(s) 472, 553, 583, 849, 1552, 1654
RsaNI GTAC 6 cut(s) 471, 552, 582, 848, 1551, 1653
SacI GAGCTC 1 cut(s) 392
SalI GTCGAC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 380
SatI GCNGC 7 cut(s) 249, 942, 1325, 1347, 1380, 1494, 1612
Sau3AI GATC 2 cut(s) 148, 818
Sau96I GGNCC 4 cut(s) 1212, 1540, 1541, 1643
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 4 cut(s) 125, 431, 1157, 1186
ScrFI CCNGG 3 cut(s) 235, 1242, 1727
SduI GDGCHC 2 cut(s) 392, 1544
SfaNI GCATC 6 cut(s) 82, 178, 616, 1034, 1222, 1353
SfcI CTRYAG 4 cut(s) 942, 996, 1554, 1609
SinI GGWCC 2 cut(s) 1212, 1643
SmlI CTYRAG 3 cut(s) 530, 733, 1706
SmoI CTYRAG 3 cut(s) 530, 733, 1706
SsiI CCGC 6 cut(s) 248, 540, 785, 1514, 1547, 1739
SspMI CTAG 6 cut(s) 165, 354, 474, 633, 1290, 1383
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 233, 1240, 1725
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 4 cut(s) 35, 123, 1534, 1555
TaiI ACGT 2 cut(s) 74, 553
TaqI TCGA 5 cut(s) 79, 85, 616, 770, 1058
TaqII GACCGA 1 cut(s) 1409
TatI WGTACW 1 cut(s) 470
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 5 cut(s) 904, 968, 1388, 1427, 1676
TscAI CASTG 4 cut(s) 49, 1027, 1090, 1539
TseFI GTSAC 4 cut(s) 117, 544, 1189, 1683
TseI GCWGC 6 cut(s) 941, 1324, 1346, 1379, 1493, 1611
Tsp45I GTSAC 4 cut(s) 117, 544, 1189, 1683
TspDTI ATGAA 6 cut(s) 237, 416, 900, 981, 1098, 1236
TspGWI ACGGA 2 cut(s) 343, 602
TspRI CASTG 4 cut(s) 49, 1027, 1090, 1539
Vha464I CTTAAG 1 cut(s) 1706
VpaK11BI GGWCC 2 cut(s) 1212, 1643
XapI RAATTY 3 cut(s) 664, 714, 1413
XbaI TCTAGA 2 cut(s) 632, 1289
XceI RCATGY 1 cut(s) 1755
XmiI GTMKAC 2 cut(s) 79, 599
XmnI GAANNNNTTC 3 cut(s) 728, 828, 991
XspI CTAG 6 cut(s) 165, 354, 474, 633, 1290, 1383
ZrmI AGTACT 1 cut(s) 472
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.