pycom03g23210

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016629
Physical Location & Seq
Forward (+)
23121333 .. 23123942
2610 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGGCGCAGACCTCCGTTTCCACCTCCGCCTCCGCACTCAGATTCAACTCCCTCGTCTTCGCCCCAAACCCTAGCTCTTCTCCCCAACCGCAGACCCTTTCTCTCCCCTTCCACCGCTTTGCTTCAAGGAATTTGAAAAATTTAGTTGGGAGCAGCAAAAGGAGTTCTCCAGTGAAAGCTATCTACTCTGGTGAATTTTGGACACCGGCGGAAAGGAATTCCCGTCAAGGAATTTGGTCCATAAGGGAGGATGTCCAAGTTCCATCTTCGCCATACTTCCCTGCATATGCACAGGGACAGGGGCCACCCCCCATGAGAATAATACGTTGTGGGGGAGCTGTTGATGATGATATGGCAAACATAATTGTTGCTCAACTTCTTTACCTTGATGCTGTTGATCCTCAGAAGGATATTGTCATGTATGTTAATTCACTAACAGGAGGATCTGTTACTGCTGGTATGGCCATTTTTGACACAATGAGGCATATCCGACCTGATGTCTCCACTGTTTGCGTTGGACTTGCTGCTAGTATGGGAGCTTTCCTGCTTAGCGCTGGTACCAAAGGAAAAAGATACAGCTTGCCAAATTCAAGGATAATGATCCATCAGCCTCTTGGTGGAGCTCAAGGTGGGCAAACTGACATTGATATTCAGGCAAATGAGATGCTACACCATAAGGCAAACTTGAATGGCTATCTCTCGTACCACACTGGTCAAAGTCTTGAAAAGATCAACCAAGACACAGATCGCGATTTCTTCATGAGTGCGAAGGAAGCCCAAGAATACGGGCTTATAGATGGTGTTATATCGAATCCTCTCAAAGCTTTCCAACCTTTGGCAGCCGCAACAGCGAGTGGAGATGAACCTGCTGAGCAGACTGTTGCGGACAGTTAA

Protein Analysis

298

Amino Acids

31.95

Weight (kDa)

6.3

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 106 - 271 1.8e-74 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016629)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02560
fragaria_vesca FvH4_5g39050
malus_domestica MD00G1140700.v1.1 MD03G1292800.v1.1
prunus_persica Prupe.8G265800_v2.0.a1
pyrus_communis pycom03g23210 pycom11g27350
rosa_chinensis RchiOBHm_Chr7g0241011
rosa_laevigata RLG00000000604
rosa_multiflora Rmu_sc0003187.1_g000009
rosa_roxburghii Rroxscaffold_3G00219320
rosa_rugosa Rorug07G0341200
rosa_samantha Rh7BG467700
rosa_wichuraiana Rw7G041930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 874
Acc65I GGTACC 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 835
AccII CGCG 1 cut(s) 750
AciI CCGC 7 cut(s) 27, 33, 89, 115, 209, 843, 884
AclWI GGATC 3 cut(s) 392, 451, 595
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 6 cut(s) 130, 139, 194, 217, 231, 586
AfaI GTAC 2 cut(s) 559, 704
AfeI AGCGCT 1 cut(s) 553
AfiI CCNNNNNNNGG 2 cut(s) 617, 835
AgsI TTSAA 6 cut(s) 46, 126, 136, 591, 688, 725
AhdI GACNNNNNGTC 1 cut(s) 497
AluBI AGCT 7 cut(s) 75, 179, 338, 539, 579, 623, 824
AluI AGCT 7 cut(s) 75, 179, 338, 539, 579, 623, 824
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 3 cut(s) 392, 451, 595
Aor51HI AGCGCT 1 cut(s) 553
AoxI GGCC 2 cut(s) 302, 462
ApeKI GCWGC 3 cut(s) 153, 524, 839
ApoI RAATTY 6 cut(s) 130, 139, 194, 217, 231, 586
Asp718I GGTACC 1 cut(s) 557
AspLEI GCGC 2 cut(s) 7, 554
AspS9I GGNCC 2 cut(s) 237, 302
AsuHPI GGTGA 1 cut(s) 203
AvaII GGWCC 1 cut(s) 237
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 3 cut(s) 165, 511, 851
BccI CCATC 3 cut(s) 271, 612, 791
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 2 cut(s) 72, 528
BfoI RGCGCY 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 874
BisI GCNGC 4 cut(s) 154, 525, 840, 843
BlpI GCTNAGC 2 cut(s) 548, 870
BlsI GCNGC 4 cut(s) 155, 526, 841, 844
Bme18I GGWCC 1 cut(s) 237
BmeRI GACNNNNNGTC 1 cut(s) 497
BmgT120I GGNCC 2 cut(s) 237, 302
BmiI GGNNCC 2 cut(s) 303, 559
BmsI GCATC 2 cut(s) 379, 654
BpiI GAAGAC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 153
Bpu1102I GCTNAGC 2 cut(s) 548, 870
BpuEI CTTGAG 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 599
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 617, 835
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 2 cut(s) 170, 715
Bse8I GATNNNNATC 1 cut(s) 599
BseGI GGATG 1 cut(s) 256
BseJI GATNNNNATC 1 cut(s) 599
BseLI CCNNNNNNNGG 2 cut(s) 617, 835
BseMII CTCAG 3 cut(s) 52, 416, 861
BseNI ACTGG 2 cut(s) 170, 715
BseXI GCAGC 3 cut(s) 165, 511, 851
Bsh1236I CGCG 1 cut(s) 750
BshFI GGCC 2 cut(s) 304, 464
BshNI GGYRCC 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 2 cut(s) 617, 835
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 2 cut(s) 304, 464
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 5 cut(s) 397, 443, 600, 729, 745
Bsp1720I GCTNAGC 2 cut(s) 548, 870
Bsp68I TCGCGA 1 cut(s) 750
BspACI CCGC 7 cut(s) 27, 33, 89, 115, 209, 843, 884
BspANI GGCC 2 cut(s) 304, 464
BspCNI CTCAG 3 cut(s) 51, 415, 862
BspFNI CGCG 1 cut(s) 750
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 2 cut(s) 303, 559
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 3 cut(s) 392, 451, 595
BspQI GCTCTTC 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 557
BsrFI RCCGGY 1 cut(s) 205
BsrI ACTGG 2 cut(s) 170, 715
BssAI RCCGGY 1 cut(s) 205
BssMI GATC 5 cut(s) 397, 443, 600, 729, 745
Bst4CI ACNGT 3 cut(s) 508, 880, 890
Bst6I CTCTTC 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 4 cut(s) 38, 402, 548, 870
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 750
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 2 cut(s) 7, 554
BstKTI GATC 5 cut(s) 400, 446, 603, 732, 748
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 5 cut(s) 397, 443, 600, 729, 745
BstMWI GCNNNNNNNGC 3 cut(s) 461, 773, 848
BstUI CGCG 1 cut(s) 750
BstV1I GCAGC 3 cut(s) 165, 511, 851
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BsuRI GGCC 2 cut(s) 304, 464
BtsCI GGATG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 177, 504, 708
BtuMI TCGCGA 1 cut(s) 750
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 1 cut(s) 581
CciI TCATGA 1 cut(s) 759
CfoI GCGC 2 cut(s) 7, 554
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 237, 302
Csp6I GTAC 2 cut(s) 558, 703
CviAII CATG 3 cut(s) 313, 418, 760
CviQI GTAC 2 cut(s) 558, 703
DdeI CTNAG 4 cut(s) 38, 402, 548, 870
DpnI GATC 5 cut(s) 399, 445, 602, 731, 747
DpnII GATC 5 cut(s) 397, 443, 600, 729, 745
DriI GACNNNNNGTC 1 cut(s) 497
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 82
Eam1105I GACNNNNNGTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 82
EciI GGCGGA 2 cut(s) 16, 224
Ecl136II GAGCTC 1 cut(s) 623
Eco24I GRGCYC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 237
Eco47III AGCGCT 1 cut(s) 553
Eco53kI GAGCTC 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 623
EcoRI GAATTC 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 625
FaeI CATG 3 cut(s) 316, 421, 763
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 1 cut(s) 309
FatI CATG 3 cut(s) 312, 417, 759
FauNDI CATATG 1 cut(s) 286
Fnu4HI GCNGC 4 cut(s) 154, 525, 840, 843
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 4 cut(s) 154, 525, 840, 843
FspBI CTAG 2 cut(s) 72, 528
GlaI GCGC 2 cut(s) 6, 553
GluI GCNGC 4 cut(s) 154, 525, 840, 843
GsuI CTGGAG 1 cut(s) 153
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 2 cut(s) 304, 464
HapII CCGG 1 cut(s) 206
HhaI GCGC 2 cut(s) 7, 554
Hin1II CATG 3 cut(s) 316, 421, 763
Hin6I GCGC 2 cut(s) 5, 552
HinP1I GCGC 2 cut(s) 5, 552
HindIII AAGCTT 1 cut(s) 822
HinfI GANTC 2 cut(s) 42, 811
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 3 cut(s) 41, 405, 491
Hpy188III TCNNGA 3 cut(s) 722, 749, 760
HpyAV CCTTC 3 cut(s) 118, 400, 763
HpyCH4III ACNGT 3 cut(s) 508, 880, 890
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 2 cut(s) 284, 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 461, 773, 848
HpyF3I CTNAG 4 cut(s) 38, 402, 548, 870
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 3 cut(s) 316, 421, 763
HspAI GCGC 2 cut(s) 5, 552
KpnI GGTACC 1 cut(s) 561
Kzo9I GATC 5 cut(s) 397, 443, 600, 729, 745
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 4 cut(s) 150, 335, 536, 620
Lsp1109I GCAGC 3 cut(s) 165, 511, 851
LweI GCATC 2 cut(s) 379, 654
MaeI CTAG 2 cut(s) 72, 528
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 448
MalI GATC 5 cut(s) 399, 445, 602, 731, 747
MboI GATC 5 cut(s) 397, 443, 600, 729, 745
MboII GAAGA 4 cut(s) 49, 69, 258, 748
MflI RGATCY 1 cut(s) 443
MhlI GDGCHC 1 cut(s) 625
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 8 cut(s) 130, 139, 194, 217, 231, 363, 427, 586
MluNI TGGCCA 1 cut(s) 464
MmeI TCCRAC 3 cut(s) 496, 514, 853
Mox20I TGGCCA 1 cut(s) 464
MscI TGGCCA 1 cut(s) 464
MseI TTAA 2 cut(s) 426, 892
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 1 cut(s) 206
MvnI CGCG 1 cut(s) 750
MwoI GCNNNNNNNGC 3 cut(s) 461, 773, 848
NdeI CATATG 1 cut(s) 286
NdeII GATC 5 cut(s) 397, 443, 600, 729, 745
NlaIII CATG 3 cut(s) 316, 421, 763
NlaIV GGNNCC 2 cut(s) 303, 559
NruI TCGCGA 1 cut(s) 750
PagI TCATGA 1 cut(s) 759
PciSI GCTCTTC 1 cut(s) 82
PfeI GAWTC 2 cut(s) 42, 811
PflMI CCANNNNNTGG 1 cut(s) 835
PkrI GCNGC 4 cut(s) 155, 526, 841, 844
Psp124BI GAGCTC 1 cut(s) 625
PspN4I GGNNCC 2 cut(s) 303, 559
PspPI GGNCC 2 cut(s) 237, 302
PsuI RGATCY 1 cut(s) 443
RruI TCGCGA 1 cut(s) 750
RsaI GTAC 2 cut(s) 559, 704
RsaNI GTAC 2 cut(s) 558, 703
SacI GAGCTC 1 cut(s) 625
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 2 cut(s) 426, 892
SatI GCNGC 4 cut(s) 154, 525, 840, 843
Sau3AI GATC 5 cut(s) 397, 443, 600, 729, 745
Sau96I GGNCC 2 cut(s) 237, 302
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 379, 654
SgrAI CRCCGGYG 1 cut(s) 205
SinI GGWCC 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
Sse9I AATT 8 cut(s) 130, 139, 194, 217, 231, 363, 427, 586
SsiI CCGC 7 cut(s) 27, 33, 89, 115, 209, 843, 884
SspMI CTAG 2 cut(s) 72, 528
SstI GAGCTC 1 cut(s) 625
TaaI ACNGT 3 cut(s) 508, 880, 890
TaiI ACGT 1 cut(s) 328
TaqI TCGA 1 cut(s) 809
TasI AATT 8 cut(s) 130, 139, 194, 217, 231, 363, 427, 586
TauI GCSGC 1 cut(s) 845
TfiI GAWTC 2 cut(s) 42, 811
Tru1I TTAA 2 cut(s) 426, 892
Tru9I TTAA 2 cut(s) 426, 892
TscAI CASTG 3 cut(s) 177, 511, 715
TseI GCWGC 3 cut(s) 153, 524, 839
TspDTI ATGAA 2 cut(s) 748, 876
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 3 cut(s) 177, 511, 715
Van91I CCANNNNNTGG 1 cut(s) 835
VpaK11BI GGWCC 1 cut(s) 237
XapI RAATTY 6 cut(s) 130, 139, 194, 217, 231, 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XspI CTAG 2 cut(s) 72, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.