Rw7G041930

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016629
Physical Location & Seq
Forward (+)
65586673 .. 65589544
2872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041930.1

Sequence Viewer

Length: 918 bp
ATGGCGCAGACCTGCGTTTCCACATCGGCCTCCTCCTCTCTCAGATTCAACTCCCTCGGCTTCGCTCTAAACCCTAGCTCTTGTTCCCAGCCACACAGCCTCACCGTCTCTCTCCCCTCTCAACGCTTTCCTTCAAGGAAGTTGAAGAAGTTAGTTGGGAATAGGAAAGGTCTCCAGAGTTCTCCGGTGAAGGCTGTCTATGATGAATTTTGGGCACCGGGTTCTCGTGAAGGAATTTGGTCCATAAGTTCCATCTTCGCCATACTTTCCTGCCTATGCACAGGGACAGGTCCGCCGCCTATGGTGCAAGAACGCTTTCAGAGTGTTATTAGTCAGCTTTTCCAGCATAGAATCATTCGTTGTGGGGGAGCTGTGGATGATGATATGGCAAATATAATTGTCGCTCAGCTTCTTTATCTTGATGCTGTTGATCCTAAAAAGGATATTGTCATGTATGTGAATTCACCTGGAGGATCTGTTACTGCTGGTATGGCCATTTTCGACACTATGAGGCATATCCGACCTGATGTCTCTACTGTGTGTGTTGGACTAGCAGCTAGTATGGGGGCTTTCCTGCTTAGCGCTGGCACCAAAGGAAAACGGTACAGCTTGCCAAATTCAAGGATAATGATCCATCAGCCTCTTGGTGGAGCTCAAGGAGGGCAAACTGACATAGATATTCAGGCAAATGAAATGCTGCATCACAAGGCAAACTTAAATGGTTATCTCTCCTACCACACTGGTCAAAGTCTTGAAAAGATCAACCAAGACACAGATCGTGATTTCTTCATGAGTGCAAAGGAGGCCAAGGAGTATGGGCTTATAGATGGTGTGATATTGAATCCTCTCAAAGCTCTCCAACCGTTGCCAGTTGCAGTAGAGAGTACTGATGAATCCAGTGAGAAAGCTGTTGCCTAG

Protein Analysis

305

Amino Acids

32.89

Weight (kDa)

8.18

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 103 - 279 4e-81 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016629)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02560
fragaria_vesca FvH4_5g39050
malus_domestica MD00G1140700.v1.1 MD03G1292800.v1.1
prunus_persica Prupe.8G265800_v2.0.a1
pyrus_communis pycom03g23210 pycom11g27350
rosa_chinensis RchiOBHm_Chr7g0241011
rosa_laevigata RLG00000000604
rosa_multiflora Rmu_sc0003187.1_g000009
rosa_roxburghii Rroxscaffold_3G00219320
rosa_rugosa Rorug07G0341200
rosa_samantha Rh7BG467700
rosa_wichuraiana Rw7G041930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 20
AccB1I GGYRCC 2 cut(s) 214, 587
AciI CCGC 2 cut(s) 293, 296
AclWI GGATC 3 cut(s) 425, 481, 625
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 4 cut(s) 206, 234, 460, 616
AfaI GTAC 2 cut(s) 605, 886
AfeI AGCGCT 1 cut(s) 583
AfiI CCNNNNNNNGG 1 cut(s) 647
AgsI TTSAA 6 cut(s) 49, 135, 145, 621, 755, 841
AhdI GACNNNNNGTC 1 cut(s) 527
AjnI CCWGG 1 cut(s) 466
AluBI AGCT 9 cut(s) 78, 337, 371, 409, 557, 609, 653, 854, 908
AluI AGCT 9 cut(s) 78, 337, 371, 409, 557, 609, 653, 854, 908
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 3 cut(s) 112, 176, 535
AlwI GGATC 3 cut(s) 425, 481, 625
Aor51HI AGCGCT 1 cut(s) 583
AoxI GGCC 3 cut(s) 27, 492, 804
ApeKI GCWGC 2 cut(s) 554, 697
ApoI RAATTY 4 cut(s) 206, 234, 460, 616
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 2 cut(s) 7, 584
AspS9I GGNCC 2 cut(s) 240, 290
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 94, 199, 456
AvaII GGWCC 2 cut(s) 240, 290
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 494
BanI GGYRCC 2 cut(s) 214, 587
BanII GRGCYC 1 cut(s) 655
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 655
BbvI GCAGC 2 cut(s) 566, 684
BccI CCATC 3 cut(s) 260, 642, 821
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 3 cut(s) 112, 176, 535
BfaI CTAG 4 cut(s) 75, 551, 558, 916
BfoI RGCGCY 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 3 cut(s) 296, 555, 698
BlpI GCTNAGC 2 cut(s) 405, 578
BlsI GCNGC 3 cut(s) 297, 556, 699
BmcAI AGTACT 1 cut(s) 886
Bme1390I CCNGG 2 cut(s) 219, 468
Bme18I GGWCC 2 cut(s) 240, 290
BmeRI GACNNNNNGTC 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 240, 290
BmiI GGNNCC 2 cut(s) 216, 589
BmrFI CCNGG 2 cut(s) 219, 468
BmsI GCATC 2 cut(s) 412, 709
BpmI CTGGAG 2 cut(s) 158, 489
Bpu1102I GCTNAGC 2 cut(s) 405, 578
BpuEI CTTGAG 1 cut(s) 639
BpuMI CCSGG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 55, 807
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse1I ACTGG 3 cut(s) 745, 869, 897
Bse8I GATNNNNATC 1 cut(s) 629
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 55, 807
BseGI GGATG 1 cut(s) 382
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 1 cut(s) 647
BseMII CTCAG 2 cut(s) 55, 419
BseNI ACTGG 3 cut(s) 745, 869, 897
BseRI GAGGAG 2 cut(s) 22, 25
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 566, 684
BseYI CCCAGC 1 cut(s) 87
BshFI GGCC 3 cut(s) 29, 494, 806
BshNI GGYRCC 2 cut(s) 214, 587
BsiHKAI GWGCWC 1 cut(s) 655
BsiSI CCGG 2 cut(s) 185, 218
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 3 cut(s) 112, 176, 535
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 3 cut(s) 29, 494, 806
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 217, 655
Bsp143I GATC 5 cut(s) 430, 473, 630, 759, 775
Bsp1720I GCTNAGC 2 cut(s) 405, 578
BspACI CCGC 2 cut(s) 293, 296
BspANI GGCC 3 cut(s) 29, 494, 806
BspCNI CTCAG 2 cut(s) 54, 418
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 2 cut(s) 216, 589
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 3 cut(s) 425, 481, 625
BspT107I GGYRCC 2 cut(s) 214, 587
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 3 cut(s) 745, 869, 897
BssECI CCNNGG 2 cut(s) 55, 807
BssMI GATC 5 cut(s) 430, 473, 630, 759, 775
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 807
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 4 cut(s) 106, 538, 603, 864
BstC8I GCNNGC 2 cut(s) 586, 611
BstDEI CTNAG 3 cut(s) 41, 405, 578
BstF5I GGATG 1 cut(s) 382
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 2 cut(s) 7, 584
BstKTI GATC 5 cut(s) 433, 476, 633, 762, 778
BstMAI GTCTC 3 cut(s) 112, 176, 535
BstMBI GATC 5 cut(s) 430, 473, 630, 759, 775
BstMWI GCNNNNNNNGC 4 cut(s) 304, 343, 491, 803
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 217, 466
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 566, 684
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BsuRI GGCC 3 cut(s) 29, 494, 806
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 738, 904
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 2 cut(s) 586, 611
CciI TCATGA 1 cut(s) 789
CfoI GCGC 2 cut(s) 7, 584
Cfr13I GGNCC 2 cut(s) 240, 290
Csp6I GTAC 2 cut(s) 604, 885
CviAII CATG 2 cut(s) 451, 790
CviQI GTAC 2 cut(s) 604, 885
DdeI CTNAG 3 cut(s) 41, 405, 578
DpnI GATC 5 cut(s) 432, 475, 632, 761, 777
DpnII GATC 5 cut(s) 430, 473, 630, 759, 775
DriI GACNNNNNGTC 1 cut(s) 527
EaeI YGGCCR 1 cut(s) 492
Eam1105I GACNNNNNGTC 1 cut(s) 527
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 653
Eco130I CCWWGG 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 655
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 240, 290
Eco47III AGCGCT 1 cut(s) 583
Eco53kI GAGCTC 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 653
EcoRI GAATTC 1 cut(s) 460
EcoRII CCWGG 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 807
EcoT38I GRGCYC 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 807
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 2 cut(s) 454, 793
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FaqI GGGAC 1 cut(s) 298
FatI CATG 2 cut(s) 450, 789
Fnu4HI GCNGC 3 cut(s) 296, 555, 698
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 655
Fsp4HI GCNGC 3 cut(s) 296, 555, 698
FspBI CTAG 4 cut(s) 75, 551, 558, 916
GlaI GCGC 2 cut(s) 6, 583
GluI GCNGC 3 cut(s) 296, 555, 698
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 2 cut(s) 158, 489
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 3 cut(s) 29, 494, 806
HapII CCGG 2 cut(s) 185, 218
HhaI GCGC 2 cut(s) 7, 584
Hin1II CATG 2 cut(s) 454, 793
Hin6I GCGC 2 cut(s) 5, 582
HinP1I GCGC 2 cut(s) 5, 582
HinfI GANTC 4 cut(s) 45, 351, 841, 893
HpaII CCGG 2 cut(s) 185, 218
HphI GGTGA 3 cut(s) 94, 199, 456
Hpy188I TCNGA 3 cut(s) 44, 321, 521
Hpy188III TCNNGA 6 cut(s) 175, 227, 419, 752, 779, 790
HpyAV CCTTC 3 cut(s) 141, 184, 224
HpyCH4III ACNGT 4 cut(s) 106, 538, 603, 864
HpyCH4V TGCA 5 cut(s) 279, 307, 700, 797, 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 343, 491, 803
HpyF3I CTNAG 3 cut(s) 41, 405, 578
Hsp92II CATG 2 cut(s) 454, 793
HspAI GCGC 2 cut(s) 5, 582
Kzo9I GATC 5 cut(s) 430, 473, 630, 759, 775
LmnI GCTCC 2 cut(s) 368, 650
Lsp1109I GCAGC 2 cut(s) 566, 684
LweI GCATC 2 cut(s) 412, 709
MaeI CTAG 4 cut(s) 75, 551, 558, 916
MaeIII GTNAC 1 cut(s) 478
MalI GATC 5 cut(s) 432, 475, 632, 761, 777
MboI GATC 5 cut(s) 430, 473, 630, 759, 775
MboII GAAGA 3 cut(s) 157, 247, 778
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 2 cut(s) 217, 655
MlsI TGGCCA 1 cut(s) 494
MluCI AATT 5 cut(s) 206, 234, 396, 460, 616
MluNI TGGCCA 1 cut(s) 494
MmeI TCCRAC 3 cut(s) 526, 544, 883
Mox20I TGGCCA 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 494
MseI TTAA 1 cut(s) 716
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 494
MspI CCGG 2 cut(s) 185, 218
MspR9I CCNGG 2 cut(s) 219, 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 4 cut(s) 304, 343, 491, 803
NciI CCSGG 1 cut(s) 219
NdeII GATC 5 cut(s) 430, 473, 630, 759, 775
NlaIII CATG 2 cut(s) 454, 793
NlaIV GGNNCC 2 cut(s) 216, 589
NmeAIII GCCGAG 1 cut(s) 36
PagI TCATGA 1 cut(s) 789
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 4 cut(s) 45, 351, 841, 893
PkrI GCNGC 3 cut(s) 297, 556, 699
Psp124BI GAGCTC 1 cut(s) 655
Psp6I CCWGG 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 216, 589
PspPI GGNCC 2 cut(s) 240, 290
PsuI RGATCY 1 cut(s) 473
RsaI GTAC 2 cut(s) 605, 886
RsaNI GTAC 2 cut(s) 604, 885
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 655
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 3 cut(s) 296, 555, 698
Sau3AI GATC 5 cut(s) 430, 473, 630, 759, 775
Sau96I GGNCC 2 cut(s) 240, 290
ScaI AGTACT 1 cut(s) 886
ScrFI CCNGG 2 cut(s) 219, 468
SduI GDGCHC 2 cut(s) 217, 655
SfaNI GCATC 2 cut(s) 412, 709
SinI GGWCC 2 cut(s) 240, 290
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 654
SmoI CTYRAG 1 cut(s) 654
Sse9I AATT 5 cut(s) 206, 234, 396, 460, 616
SsiI CCGC 2 cut(s) 293, 296
SspMI CTAG 4 cut(s) 75, 551, 558, 916
SstI GAGCTC 1 cut(s) 655
StyD4I CCNGG 2 cut(s) 217, 466
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 4 cut(s) 106, 538, 603, 864
TaqI TCGA 1 cut(s) 501
TasI AATT 5 cut(s) 206, 234, 396, 460, 616
TatI WGTACW 1 cut(s) 884
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 4 cut(s) 45, 351, 841, 893
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 2 cut(s) 745, 904
TseI GCWGC 2 cut(s) 554, 697
TspDTI ATGAA 4 cut(s) 219, 705, 778, 906
TspRI CASTG 2 cut(s) 745, 904
VpaK11BI GGWCC 2 cut(s) 240, 290
XapI RAATTY 4 cut(s) 206, 234, 460, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 4 cut(s) 75, 551, 558, 916
ZrmI AGTACT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.