pycom04g01380

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013239
Physical Location & Seq
Reverse (-)
1280813 .. 1283480
2668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1683 bp
ATGACCAATCAGAAAAGATGTATTGTTCTGTATTTGAATGATGACGTGGCGACAAACATACGCGTTCAAGTCAATTCTGTCAACCGGCAGCGGCTCGACTCCAACGTCCTCTCTCTCTCTCTCTCTCCAGTCTGTCGTCAAATTTGTTCCATTTCTCTCGAACGTTCCAATCGGATCCCAAAATCGATTCCAATCCAGTCCGCCCAAACCCTGACCCGACTCTCCATTATGGAAGGAGGCTCGACTGCCGGCGGAGGCGGTGGCCCGGCGCCGTTTCTGCTGAAGACGTACAACATGGTGGACGATTCCTCGACGGACGAGATCGTGTCGTGGAGCTCGAACAAGAAGAGCTTCGTCGTCTGGAACCCGCCCGAGTTCGCCCGGGTTCTGCTCCCCACCTATTTCAAGCACAACAACTTCTCCAGCTTCATTCGTCAGCTCAATACATACGGATTTCGAAAGAGTGATCGATCTGAGAGATGGGAATTTGCTAATGAAGACTTCATACAAGATCAGAAGCATCTTCTTAAGAATATCCACCGCAGAAAACCCATTCACAGCCACAGCAATCCTGAAGGTTTTATGGTCGATCCAGAAAGAGCAGCTCTTGATGACGAAATAGAGAAGCTTTCACATGATAAAGCTGCACTCGAGGCAAGTATTTCAACATTCAAACAGCAGCGATCTGCAGCTAAGCTTCAGTTGGAAAACTTAGCACAGCGGGTGAGTGGTATGGAACAACGGCAGAAGAATTTGCTGACATTCTTAGATGAGGCTGTTCACAACCCTAATTTTATTGAACACCTCTCTAGAAAAATTGAATCTATGGATTTCTCAGCATATAATAAGAAAAGGAGATTGCCTGACATTGAAAACCTACAGCCAGTTGTGGAGAATGGTTTTGTGGATAACCAAAGTAGTTCCATATCTGAGTTTGGAAATGTTTTCCACCAAGACTTCTCAAGTAAACTTAGACTAGAATTATCACCAGCTGTTTCAGACATGAATTTGGTTTCGCTTAGCACGCAGAGTTCCAATGAAGATGGGGGTAGTTCAACTAGGAAAATATCTGAAGAACTTAAAGGTGCACAGATGAGAACAGAAGGGCTTTTATTTGCACCTGAAACCTTAGAACTTTCAGATACCGGAACATCTTTTGCATTCAAAATGGATTCGTTGTTATCACGGAAGGCACCAACTGTTGGAAGCCCTATACGTCATTCCTTGCAGCCAGGTTTGCCTTCTAATGAAGAAGGTGATGGTCATATATCCTGCCAGTTAAATCTCACTCTTGCATCTACTCCGTCGCAAGTCAATAATAGTCCTTATTCAGCTGCAGTAACCCAAGTAGGTCAGGATATCAACAAATCTGCAGCATCAGAGCTACATGACAAGGGCAAAGAACCAGATTTAAGAGCCTTTACAAACAAAAATCTAGCTGACGATGACAAACCTAAAACTTCCTCTCAAGAAGCCACACATAACAATCAAGCGCCTGCACTGGTTCCTGTTAGAGTAAATGATGTTTTCTGGGAACAGTTCCTAACTGAAAGACCTGGCTGTTCAGAGAATGAAGAAGCGAGTTCTAATTATAGGGGAAATCCATATGATGAGCAAGATGATGGAAGGTTCGGCCATGGAATGTCCAGAACTGTCAAGGATGCGGAAACACTCACTCTTTAA

Protein Analysis

561

Amino Acids

62.51

Weight (kDa)

5.85

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 92 - 182 6.3e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13980
fragaria_vesca FvH4_4g13230
malus_domestica MD00G1118600.v1.1 MD04G1017400.v1.1
prunus_persica Prupe.1G165500_v2.0.a1
pyrus_communis pycom04g01380 pycom420g00940
rosa_chinensis RchiOBHm_Chr4g0413481
rosa_laevigata RLG00000008216
rosa_multiflora Rmu_sc0000883.1_g000011
rosa_roxburghii Rroxscaffold_5G00357380
rosa_rugosa Rorug04G0120600
rosa_samantha Rh4AG180300 Rh4BG179600 Rh4CG191300 Rh4DG175500
rosa_wichuraiana Rw4G015010 Rw4G015070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
AccB1I GGYRCC 2 cut(s) 268, 1192
AccB7I CCANNNNNTGG 1 cut(s) 1202
AccII CGCG 1 cut(s) 63
AciI CCGC 8 cut(s) 91, 201, 252, 258, 368, 541, 721, 1664
AclI AACGTT 1 cut(s) 163
AclWI GGATC 3 cut(s) 169, 182, 584
AcoI YGGCCR 1 cut(s) 1633
AcsI RAATTY 4 cut(s) 141, 485, 751, 1006
AcuI CTGAAG 4 cut(s) 302, 594, 683, 1092
AcyI GRCGYC 1 cut(s) 269
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 1 cut(s) 1202
AflII CTTAAG 1 cut(s) 527
AflIII ACRYGT 1 cut(s) 61
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 1231, 1555
Alw21I GWGCWC 2 cut(s) 338, 1090
Alw44I GTGCAC 1 cut(s) 1086
AlwI GGATC 3 cut(s) 169, 182, 584
Ama87I CYCGRG 3 cut(s) 371, 381, 650
AoxI GGCC 2 cut(s) 262, 1633
ApaLI GTGCAC 1 cut(s) 1086
ApeKI GCWGC 8 cut(s) 88, 602, 644, 679, 689, 1228, 1334, 1373
ApoI RAATTY 4 cut(s) 141, 485, 751, 1006
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 2 cut(s) 271, 1495
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 3 cut(s) 266, 382, 383
AsuHPI GGTGA 3 cut(s) 736, 978, 1268
AsuII TTCGAA 1 cut(s) 457
AvaI CYCGRG 3 cut(s) 371, 381, 650
BaeGI GKGCMC 1 cut(s) 1090
BamHI GGATCC 1 cut(s) 174
BanI GGYRCC 2 cut(s) 268, 1192
BanII GRGCYC 1 cut(s) 338
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbsI GAAGAC 2 cut(s) 290, 504
Bbv12I GWGCWC 2 cut(s) 338, 1090
BbvI GCAGC 8 cut(s) 100, 614, 631, 691, 701, 1240, 1321, 1385
BccI CCATC 4 cut(s) 474, 1037, 1253, 1616
BceAI ACGGC 2 cut(s) 256, 758
BciT130I CCWGG 2 cut(s) 1233, 1557
BcnI CCSGG 3 cut(s) 266, 382, 383
BfaI CTAG 4 cut(s) 810, 977, 1059, 1436
BfmI CTRYAG 4 cut(s) 687, 878, 1335, 1371
BfoI RGCGCY 2 cut(s) 272, 1496
BfrI CTTAAG 1 cut(s) 527
BisI GCNGC 9 cut(s) 89, 92, 603, 645, 680, 690, 1229, 1335, 1374
BlpI GCTNAGC 2 cut(s) 693, 1019
BlsI GCNGC 9 cut(s) 90, 93, 604, 646, 681, 691, 1230, 1336, 1375
Bme1390I CCNGG 5 cut(s) 266, 382, 383, 1233, 1557
BmeT110I CYCGRG 3 cut(s) 371, 381, 650
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 5 cut(s) 176, 270, 365, 1194, 1506
BmrFI CCNGG 5 cut(s) 266, 382, 383, 1233, 1557
BmsI GCATC 4 cut(s) 529, 1304, 1385, 1651
BpiI GAAGAC 2 cut(s) 290, 504
BpmI CTGGAG 2 cut(s) 111, 406
Bpu1102I GCTNAGC 2 cut(s) 693, 1019
Bpu14I TTCGAA 1 cut(s) 457
BpuEI CTTGAG 2 cut(s) 946, 1452
BpuMI CCSGG 3 cut(s) 266, 382, 383
Bsa29I ATCGAT 2 cut(s) 185, 469
BsaBI GATNNNNATC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 381, 1636
BsaWI WCCGGW 1 cut(s) 1145
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 1202
Bse118I RCCGGY 2 cut(s) 84, 248
Bse1I ACTGG 5 cut(s) 128, 196, 884, 1276, 1506
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 2 cut(s) 1233, 1557
BseCI ATCGAT 2 cut(s) 185, 469
BseDI CCNNGG 2 cut(s) 381, 1636
BseGI GGATG 1 cut(s) 1666
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 1 cut(s) 1202
BseMII CTCAG 3 cut(s) 465, 849, 921
BseNI ACTGG 5 cut(s) 128, 196, 884, 1276, 1506
BseSI GKGCMC 1 cut(s) 1090
BseXI GCAGC 8 cut(s) 100, 614, 631, 691, 701, 1240, 1321, 1385
BsgI GTGCAG 2 cut(s) 630, 1482
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 264, 1635
BshNI GGYRCC 2 cut(s) 268, 1192
BshVI ATCGAT 2 cut(s) 185, 469
BsiHKAI GWGCWC 2 cut(s) 338, 1090
BsiHKCI CYCGRG 3 cut(s) 371, 381, 650
BsiSI CCGG 5 cut(s) 85, 249, 266, 382, 1146
BslI CCNNNNNNNGG 1 cut(s) 1202
BsmI GAATGC 1 cut(s) 1160
BsnI GGCC 2 cut(s) 264, 1635
BsoBI CYCGRG 3 cut(s) 371, 381, 650
Bsp119I TTCGAA 1 cut(s) 457
Bsp1286I GDGCHC 2 cut(s) 338, 1090
Bsp143I GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
Bsp1720I GCTNAGC 2 cut(s) 693, 1019
Bsp19I CCATGG 1 cut(s) 1636
BspACI CCGC 8 cut(s) 91, 201, 252, 258, 368, 541, 721, 1664
BspANI GGCC 2 cut(s) 264, 1635
BspCNI CTCAG 3 cut(s) 466, 848, 922
BspDI ATCGAT 2 cut(s) 185, 469
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 5 cut(s) 176, 270, 365, 1194, 1506
BspMAI CTGCAG 3 cut(s) 691, 1339, 1375
BspPI GGATC 3 cut(s) 169, 182, 584
BspQI GCTCTTC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 457
BspT107I GGYRCC 2 cut(s) 268, 1192
BspTI CTTAAG 1 cut(s) 527
BsrFI RCCGGY 2 cut(s) 84, 248
BsrI ACTGG 5 cut(s) 128, 196, 884, 1276, 1506
BssAI RCCGGY 2 cut(s) 84, 248
BssECI CCNNGG 2 cut(s) 381, 1636
BssMI GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
BssNI GRCGYC 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 1636
Bst2UI CCWGG 2 cut(s) 1233, 1557
Bst4CI ACNGT 3 cut(s) 1201, 1539, 1654
Bst6I CTCTTC 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 269
BstAFI CTTAAG 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 3 cut(s) 250, 1025, 1497
BstDEI CTNAG 9 cut(s) 474, 693, 712, 766, 835, 930, 971, 1019, 1129
BstDSI CCRYGG 1 cut(s) 1636
BstF5I GGATG 1 cut(s) 1666
BstFNI CGCG 1 cut(s) 63
BstH2I RGCGCY 2 cut(s) 272, 1496
BstHHI GCGC 2 cut(s) 271, 1495
BstKTI GATC 7 cut(s) 177, 324, 469, 473, 514, 592, 686
BstMBI GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
BstMWI GCNNNNNNNGC 4 cut(s) 277, 653, 1024, 1237
BstNI CCWGG 2 cut(s) 1233, 1557
BstSCI CCNGG 5 cut(s) 264, 380, 381, 1231, 1555
BstSFI CTRYAG 4 cut(s) 687, 878, 1335, 1371
BstSLI GKGCMC 1 cut(s) 1090
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 8 cut(s) 100, 614, 631, 691, 701, 1240, 1321, 1385
BstV2I GAAGAC 2 cut(s) 290, 504
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
Bsu15I ATCGAT 2 cut(s) 185, 469
BsuRI GGCC 2 cut(s) 264, 1635
BsuTUI ATCGAT 2 cut(s) 185, 469
BtgI CCRYGG 1 cut(s) 1636
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 1 cut(s) 1666
BtsIMutI CAGTG 1 cut(s) 1499
Cac8I GCNNGC 3 cut(s) 250, 1025, 1497
CfoI GCGC 2 cut(s) 271, 1495
Cfr10I RCCGGY 2 cut(s) 84, 248
Cfr13I GGNCC 1 cut(s) 263
Cfr9I CCCGGG 1 cut(s) 381
ClaI ATCGAT 2 cut(s) 185, 469
Csp6I GTAC 1 cut(s) 289
CviAII CATG 5 cut(s) 295, 635, 1003, 1388, 1637
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 9 cut(s) 474, 693, 712, 766, 835, 930, 971, 1019, 1129
DinI GGCGCC 1 cut(s) 270
DpnI GATC 7 cut(s) 176, 323, 468, 472, 513, 591, 685
DpnII GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 1633
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
EciI GGCGGA 2 cut(s) 190, 267
Ecl136II GAGCTC 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 1636
Eco24I GRGCYC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 1360
Eco53kI GAGCTC 1 cut(s) 336
Eco57I CTGAAG 4 cut(s) 302, 594, 683, 1092
Eco88I CYCGRG 3 cut(s) 371, 381, 650
EcoICRI GAGCTC 1 cut(s) 336
EcoRII CCWGG 2 cut(s) 1231, 1555
EcoRV GATATC 1 cut(s) 1360
EcoT14I CCWWGG 1 cut(s) 1636
EcoT38I GRGCYC 1 cut(s) 338
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 1636
FaeI CATG 5 cut(s) 298, 638, 1006, 1391, 1640
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FatI CATG 5 cut(s) 294, 634, 1002, 1387, 1636
FauI CCCGC 2 cut(s) 375, 714
FauNDI CATATG 1 cut(s) 1606
Fnu4HI GCNGC 9 cut(s) 89, 92, 603, 645, 680, 690, 1229, 1335, 1374
FokI GGATG 1 cut(s) 1673
FriOI GRGCYC 1 cut(s) 338
Fsp4HI GCNGC 9 cut(s) 89, 92, 603, 645, 680, 690, 1229, 1335, 1374
FspBI CTAG 4 cut(s) 810, 977, 1059, 1436
GlaI GCGC 2 cut(s) 270, 1494
GluI GCNGC 9 cut(s) 89, 92, 603, 645, 680, 690, 1229, 1335, 1374
GsuI CTGGAG 2 cut(s) 111, 406
HaeII RGCGCY 2 cut(s) 272, 1496
HaeIII GGCC 2 cut(s) 264, 1635
HapII CCGG 5 cut(s) 85, 249, 266, 382, 1146
HhaI GCGC 2 cut(s) 271, 1495
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 5 cut(s) 298, 638, 1006, 1391, 1640
Hin6I GCGC 2 cut(s) 269, 1493
HinP1I GCGC 2 cut(s) 269, 1493
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 2 cut(s) 626, 695
HinfI GANTC 6 cut(s) 98, 187, 219, 305, 821, 1172
HpaII CCGG 5 cut(s) 85, 249, 266, 382, 1146
HphI GGTGA 3 cut(s) 736, 978, 1268
Hpy166II GTNNAC 5 cut(s) 82, 301, 781, 968, 1088
Hpy188III TCNNGA 9 cut(s) 158, 361, 572, 593, 608, 810, 1355, 1469, 1647
Hpy8I GTNNAC 5 cut(s) 82, 301, 781, 968, 1088
Hpy99I CGWCG 3 cut(s) 316, 359, 1309
HpyAV CCTTC 7 cut(s) 227, 569, 1097, 1183, 1247, 1251, 1620
HpyCH4III ACNGT 3 cut(s) 1201, 1539, 1654
HpyCH4IV ACGT 5 cut(s) 45, 105, 163, 287, 1216
HpyF10VI GCNNNNNNNGC 4 cut(s) 277, 653, 1024, 1237
HpyF3I CTNAG 9 cut(s) 474, 693, 712, 766, 835, 930, 971, 1019, 1129
HpySE526I ACGT 5 cut(s) 45, 105, 163, 287, 1216
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 5 cut(s) 298, 638, 1006, 1391, 1640
HspAI GCGC 2 cut(s) 269, 1493
KasI GGCGCC 1 cut(s) 268
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 2 cut(s) 333, 396
Lsp1109I GCAGC 8 cut(s) 100, 614, 631, 691, 701, 1240, 1321, 1385
LweI GCATC 4 cut(s) 529, 1304, 1385, 1651
MaeI CTAG 4 cut(s) 810, 977, 1059, 1436
MaeII ACGT 5 cut(s) 45, 105, 163, 287, 1216
MaeIII GTNAC 1 cut(s) 1339
MalI GATC 7 cut(s) 176, 323, 468, 472, 513, 591, 685
MboI GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
MboII GAAGA 9 cut(s) 295, 358, 509, 515, 760, 1052, 1085, 1262, 1586
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 338, 1090
MluCI AATT 9 cut(s) 73, 141, 485, 751, 790, 816, 980, 1006, 1588
MluI ACGCGT 1 cut(s) 61
Mly113I GGCGCC 1 cut(s) 269
MlyI GAGTC 2 cut(s) 92, 213
MmeI TCCRAC 3 cut(s) 126, 684, 1183
MnlI CCTC 8 cut(s) 119, 230, 248, 319, 646, 766, 815, 1474
MroNI GCCGGC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 5 cut(s) 528, 1080, 1280, 1412, 1681
MspA1I CMGCKG 4 cut(s) 91, 721, 992, 1334
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 5 cut(s) 85, 249, 266, 382, 1146
MspR9I CCNGG 5 cut(s) 266, 382, 383, 1233, 1557
Mva1269I GAATGC 1 cut(s) 1160
MvaI CCWGG 2 cut(s) 1233, 1557
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 4 cut(s) 277, 653, 1024, 1237
NaeI GCCGGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 269
NciI CCSGG 3 cut(s) 266, 382, 383
NcoI CCATGG 1 cut(s) 1636
NdeI CATATG 1 cut(s) 1606
NdeII GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 5 cut(s) 298, 638, 1006, 1391, 1640
NlaIV GGNNCC 5 cut(s) 176, 270, 365, 1194, 1506
NspV TTCGAA 1 cut(s) 457
PaeR7I CTCGAG 1 cut(s) 650
PciSI GCTCTTC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 335
PctI GAATGC 1 cut(s) 1160
PdiI GCCGGC 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 4 cut(s) 187, 305, 821, 1172
PflMI CCANNNNNTGG 1 cut(s) 1202
PkrI GCNGC 9 cut(s) 90, 93, 604, 646, 681, 691, 1230, 1336, 1375
PleI GAGTC 2 cut(s) 92, 213
PluTI GGCGCC 1 cut(s) 272
PpsI GAGTC 2 cut(s) 92, 213
Psp124BI GAGCTC 1 cut(s) 338
Psp1406I AACGTT 1 cut(s) 163
Psp6I CCWGG 2 cut(s) 1231, 1555
PspGI CCWGG 2 cut(s) 1231, 1555
PspN4I GGNNCC 5 cut(s) 176, 270, 365, 1194, 1506
PspPI GGNCC 1 cut(s) 263
PspXI VCTCGAGB 1 cut(s) 650
PstI CTGCAG 3 cut(s) 691, 1339, 1375
PsuI RGATCY 1 cut(s) 174
PvuII CAGCTG 2 cut(s) 992, 1334
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 5 cut(s) 528, 1080, 1280, 1412, 1681
SatI GCNGC 9 cut(s) 89, 92, 603, 645, 680, 690, 1229, 1335, 1374
Sau3AI GATC 7 cut(s) 174, 321, 466, 470, 511, 589, 683
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 92, 213
ScrFI CCNGG 5 cut(s) 266, 382, 383, 1233, 1557
SduI GDGCHC 2 cut(s) 338, 1090
SfaNI GCATC 4 cut(s) 529, 1304, 1385, 1651
SfcI CTRYAG 4 cut(s) 687, 878, 1335, 1371
SfoI GGCGCC 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 650
SfuI TTCGAA 1 cut(s) 457
SlaI CTCGAG 1 cut(s) 650
SmaI CCCGGG 1 cut(s) 383
SmlI CTYRAG 4 cut(s) 527, 650, 961, 1467
SmoI CTYRAG 4 cut(s) 527, 650, 961, 1467
Sse9I AATT 9 cut(s) 73, 141, 485, 751, 790, 816, 980, 1006, 1588
SsiI CCGC 8 cut(s) 91, 201, 252, 258, 368, 541, 721, 1664
SspDI GGCGCC 1 cut(s) 268
SspMI CTAG 4 cut(s) 810, 977, 1059, 1436
SstI GAGCTC 1 cut(s) 338
StyD4I CCNGG 5 cut(s) 264, 380, 381, 1231, 1555
StyI CCWWGG 1 cut(s) 1636
TaaI ACNGT 3 cut(s) 1201, 1539, 1654
TaiI ACGT 5 cut(s) 48, 108, 166, 290, 1219
TasI AATT 9 cut(s) 73, 141, 485, 751, 790, 816, 980, 1006, 1588
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 4 cut(s) 187, 305, 821, 1172
Tru1I TTAA 5 cut(s) 528, 1080, 1280, 1412, 1681
Tru9I TTAA 5 cut(s) 528, 1080, 1280, 1412, 1681
TscAI CASTG 1 cut(s) 1506
TseI GCWGC 8 cut(s) 88, 602, 644, 679, 689, 1228, 1334, 1373
TspDTI ATGAA 7 cut(s) 418, 493, 510, 1019, 1053, 1263, 1587
TspGWI ACGGA 4 cut(s) 329, 465, 1201, 1293
TspMI CCCGGG 1 cut(s) 381
TspRI CASTG 1 cut(s) 1506
Van91I CCANNNNNTGG 1 cut(s) 1202
Vha464I CTTAAG 1 cut(s) 527
VneI GTGCAC 1 cut(s) 1086
XapI RAATTY 4 cut(s) 141, 485, 751, 1006
XbaI TCTAGA 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 650
XmaI CCCGGG 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 4 cut(s) 810, 977, 1059, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.