pycom04g12620

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010731
Physical Location & Seq
Reverse (-)
15524823 .. 15526472
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGGAAGCTCGAGTTGGGGTGGTGGTGGAAGGAAAGCAGAGAGCTTCGAATTTAGCGCAGGGAGGCGTGGTGGATGGGGGCGCCAGGAAATTCTTGCAGCAGCAGAGCCAGGCGAAGCAGTCTCTGAATCAGCAGTCCCAGATAGGGACGGTGAACCAGCTCTTAGCCGGCGGTATCGCCGGTGCTTTTGGAAAAACCTGCACTGCACCTCTTGCTCGCCTCACCATCCTCTTTCAGGTGCAAGGAATGCACTCTGACATTGCAGCATTGAGTAAGGCTAGCATATGGCATGAGGCTTCACGTATTATCAATGAAGAAGGGTTTAAAGCATTTTGGAGAGGCAATCTCGTTACCATTGCTCATCGTCTTCCGTATTCTGCCGTCAGCTTCTATGCTTATGAACGCTACAAGACCTTGCTGCATTCACTTGGTGGTGAAAATTTTAGGGGTAATGCAAGTACTGACATGTGTGTGCACTTTCTCGGTGGTGGGATGGCAGGGTTAACCGCTGCCTCAGCCACATATCCATCGATCTTGTGA

Protein Analysis

180

Amino Acids

19.1

Weight (kDa)

9.59

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 48 - 141 3.7e-22 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 2 cut(s) 171, 507
AcsI RAATTY 3 cut(s) 49, 89, 439
AcyI GRCGYC 1 cut(s) 81
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 2 cut(s) 144, 235
AflIII ACRYGT 1 cut(s) 465
AjnI CCWGG 2 cut(s) 83, 108
AjuI GAANNNNNNNTTGG 1 cut(s) 29
AluBI AGCT 4 cut(s) 8, 44, 160, 387
AluI AGCT 4 cut(s) 8, 44, 160, 387
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 1 cut(s) 126
Alw44I GTGCAC 1 cut(s) 473
AlwNI CAGNNNCTG 1 cut(s) 124
Ama87I CYCGRG 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 473
ApeKI GCWGC 5 cut(s) 97, 100, 263, 418, 509
ApoI RAATTY 3 cut(s) 49, 89, 439
AspLEI GCGC 2 cut(s) 58, 83
AsuHPI GGTGA 3 cut(s) 163, 214, 446
AsuII TTCGAA 1 cut(s) 47
AsuNHI GCTAGC 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 9
BaeGI GKGCMC 1 cut(s) 477
BanI GGYRCC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 477
BbvCI CCTCAGC 1 cut(s) 514
BbvI GCAGC 5 cut(s) 109, 112, 275, 405, 496
BccI CCATC 4 cut(s) 68, 233, 487, 535
BceAI ACGGC 1 cut(s) 365
BciT130I CCWGG 2 cut(s) 85, 110
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 5 cut(s) 98, 101, 264, 419, 510
BlsI GCNGC 5 cut(s) 99, 102, 265, 420, 511
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 2 cut(s) 85, 110
BmeT110I CYCGRG 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 82
BmrFI CCNGG 2 cut(s) 85, 110
BmtI GCTAGC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 359
BplI GAGNNNNNCTC 2 cut(s) 330, 362
Bpu10I CCTNAGC 1 cut(s) 514
Bpu14I TTCGAA 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 235
Bse118I RCCGGY 2 cut(s) 167, 179
Bse3DI GCAATG 2 cut(s) 258, 354
BseBI CCWGG 2 cut(s) 85, 110
BseCI ATCGAT 1 cut(s) 530
BseGI GGATG 3 cut(s) 79, 225, 498
BseLI CCNNNNNNNGG 2 cut(s) 144, 235
BseMI GCAATG 2 cut(s) 258, 354
BseMII CTCAG 1 cut(s) 528
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 5 cut(s) 109, 112, 275, 405, 496
BsgI GTGCAG 2 cut(s) 184, 189
BshNI GGYRCC 1 cut(s) 80
BshVI ATCGAT 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 168, 180
BslFI GGGAC 2 cut(s) 121, 160
BslI CCNNNNNNNGG 2 cut(s) 144, 235
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 2 cut(s) 121, 160
BsmI GAATGC 2 cut(s) 252, 421
BsoBI CYCGRG 1 cut(s) 9
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 1 cut(s) 531
BspACI CCGC 2 cut(s) 171, 507
BspCNI CTCAG 1 cut(s) 527
BspDI ATCGAT 1 cut(s) 530
BspLI GGNNCC 1 cut(s) 82
BspMI ACCTGC 1 cut(s) 206
BspOI GCTAGC 1 cut(s) 282
BspT104I TTCGAA 1 cut(s) 47
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 2 cut(s) 258, 354
BsrFI RCCGGY 2 cut(s) 167, 179
BssAI RCCGGY 2 cut(s) 167, 179
BssMI GATC 1 cut(s) 531
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 85, 110
Bst4CI ACNGT 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 2 cut(s) 212, 247
BstBAI YACGTR 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 3 cut(s) 169, 217, 280
BstDEI CTNAG 2 cut(s) 163, 514
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 3 cut(s) 79, 225, 498
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 58, 83
BstKTI GATC 1 cut(s) 534
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 531
BstMWI GCNNNNNNNGC 3 cut(s) 212, 247, 515
BstNI CCWGG 2 cut(s) 85, 110
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 83, 108
BstSLI GKGCMC 1 cut(s) 477
BstV1I GCAGC 5 cut(s) 109, 112, 275, 405, 496
BstV2I GAAGAC 1 cut(s) 359
Bsu15I ATCGAT 1 cut(s) 530
BsuTUI ATCGAT 1 cut(s) 530
BtsCI GGATG 3 cut(s) 79, 225, 498
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 3 cut(s) 169, 217, 280
CaiI CAGNNNCTG 1 cut(s) 124
CfoI GCGC 2 cut(s) 58, 83
Cfr10I RCCGGY 2 cut(s) 167, 179
ClaI ATCGAT 1 cut(s) 530
Csp6I GTAC 1 cut(s) 459
CviAII CATG 2 cut(s) 290, 466
CviJI RGCY 9 cut(s) 8, 44, 108, 160, 167, 278, 296, 387, 518
CviKI_1 RGCY 9 cut(s) 8, 44, 108, 160, 167, 278, 296, 387, 518
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 2 cut(s) 163, 514
DinI GGCGCC 1 cut(s) 82
DpnI GATC 1 cut(s) 533
DpnII GATC 1 cut(s) 531
DraI TTTAAA 1 cut(s) 325
DraIII CACNNNGTG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 83, 108
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
FaeI CATG 2 cut(s) 293, 469
FaiI YATR 7 cut(s) 284, 286, 291, 393, 399, 467, 523
FaqI GGGAC 2 cut(s) 121, 160
FatI CATG 2 cut(s) 289, 465
FauNDI CATATG 1 cut(s) 284
Fnu4HI GCNGC 5 cut(s) 98, 101, 264, 419, 510
FokI GGATG 3 cut(s) 86, 212, 505
Fsp4HI GCNGC 5 cut(s) 98, 101, 264, 419, 510
FspBI CTAG 1 cut(s) 279
GlaI GCGC 2 cut(s) 57, 82
GluI GCNGC 5 cut(s) 98, 101, 264, 419, 510
HaeII RGCGCY 1 cut(s) 84
HapII CCGG 2 cut(s) 168, 180
HhaI GCGC 2 cut(s) 58, 83
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 2 cut(s) 293, 469
Hin6I GCGC 2 cut(s) 56, 81
HinP1I GCGC 2 cut(s) 56, 81
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HinfI GANTC 1 cut(s) 127
HpaI GTTAAC 1 cut(s) 504
HpaII CCGG 2 cut(s) 168, 180
HphI GGTGA 3 cut(s) 163, 214, 446
Hpy166II GTNNAC 3 cut(s) 154, 475, 504
Hpy188I TCNGA 2 cut(s) 126, 256
Hpy8I GTNNAC 3 cut(s) 154, 475, 504
HpyAV CCTTC 2 cut(s) 23, 311
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 9 cut(s) 97, 201, 206, 241, 250, 263, 421, 455, 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 247, 515
HpyF3I CTNAG 2 cut(s) 163, 514
HpySE526I ACGT 1 cut(s) 301
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 2 cut(s) 293, 469
HspAI GCGC 2 cut(s) 56, 81
KasI GGCGCC 1 cut(s) 80
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
KspAI GTTAAC 1 cut(s) 504
Kzo9I GATC 1 cut(s) 531
Lsp1109I GCAGC 5 cut(s) 109, 112, 275, 405, 496
MaeI CTAG 1 cut(s) 279
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 1 cut(s) 349
MalI GATC 1 cut(s) 533
MboI GATC 1 cut(s) 531
MboII GAAGA 2 cut(s) 326, 359
MhlI GDGCHC 1 cut(s) 477
MluCI AATT 3 cut(s) 49, 89, 439
Mly113I GGCGCC 1 cut(s) 81
MnlI CCTC 7 cut(s) 56, 219, 230, 239, 286, 332, 523
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 2 cut(s) 324, 503
MslI CAYNNNNRTG 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 2 cut(s) 168, 180
MspR9I CCNGG 2 cut(s) 85, 110
Mva1269I GAATGC 2 cut(s) 252, 421
MvaI CCWGG 2 cut(s) 85, 110
MwoI GCNNNNNNNGC 3 cut(s) 212, 247, 515
NaeI GCCGGC 1 cut(s) 169
NarI GGCGCC 1 cut(s) 81
NdeI CATATG 1 cut(s) 284
NdeII GATC 1 cut(s) 531
NgoMIV GCCGGC 1 cut(s) 167
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 2 cut(s) 293, 469
NlaIV GGNNCC 1 cut(s) 82
NspI RCATGY 1 cut(s) 469
NspV TTCGAA 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 9
PciI ACATGT 1 cut(s) 465
PctI GAATGC 2 cut(s) 252, 421
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 5 cut(s) 99, 102, 265, 420, 511
PluTI GGCGCC 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 302
PscI ACATGT 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 83, 108
PspGI CCWGG 2 cut(s) 83, 108
PspN4I GGNNCC 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 124
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 2 cut(s) 324, 503
SatI GCNGC 5 cut(s) 98, 101, 264, 419, 510
Sau3AI GATC 1 cut(s) 531
ScaI AGTACT 1 cut(s) 460
ScrFI CCNGG 2 cut(s) 85, 110
SduI GDGCHC 1 cut(s) 477
SetI ASST 9 cut(s) 10, 46, 162, 200, 211, 240, 304, 389, 416
SfoI GGCGCC 1 cut(s) 82
Sfr274I CTCGAG 1 cut(s) 9
SfuI TTCGAA 1 cut(s) 47
SgrAI CRCCGGYG 1 cut(s) 179
SlaI CTCGAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 3 cut(s) 49, 89, 439
SsiI CCGC 2 cut(s) 171, 507
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 83, 108
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 1 cut(s) 304
TaqI TCGA 3 cut(s) 10, 47, 530
TasI AATT 3 cut(s) 49, 89, 439
TatI WGTACW 1 cut(s) 458
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 324, 503
Tru9I TTAA 2 cut(s) 324, 503
TscAI CASTG 1 cut(s) 208
TseI GCWGC 5 cut(s) 97, 100, 263, 418, 509
TspDTI ATGAA 2 cut(s) 327, 414
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 1 cut(s) 208
VneI GTGCAC 1 cut(s) 473
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 3 cut(s) 49, 89, 439
XceI RCATGY 1 cut(s) 469
XhoI CTCGAG 1 cut(s) 9
XspI CTAG 1 cut(s) 279
ZrmI AGTACT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.