Rorug03G0074200

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010731
Physical Location & Seq
Forward (+)
5849377 .. 5852727
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0074200.1

Sequence Viewer

Length: 606 bp
ATGGCCAGCAATGGTGCTGTCGGAGTAGATAATACTCATAGAAGGAAATTCGATCTTGAAGAGTATGCTGAGAAAGCCCGCGAGCGTGAAAGAAAGGAAGAGGAGGGAGCTCGTAAGTCGAAATCGAAAGGTCCTCCGGTGCAGAGGAAGCCATTGAAGCACAGGGATTATGAAGTAGACCTTGAATCTCGCTTGGGGAAAACACAGGTTGTTACTCCAATAGCACCTTTAAGCCAGCAGGCTGGATACTTTTGTTCAGTTTGTGAGTGCGTTGTAAAAGATTCTGCAAACTACTTGGATCATATCAATGGCAAAAAACATCAAAGAGCTTTGGGTATGTCTATGCGGGTAGAGCGGGCATCTCTTGAACAGGTCCAGAGTCGATTTGAAAAGCTCAAGAAGCGGAAAACTGATCTGCCCTTCACTGAGCAAGATCTTGATGAACGGATCTTGAAGCAGCAGCAAGAAGAAGAGGAAAGGAAGCGACAACGTCGAGAAAAGAAGAAAGAGAAGAAGAAAGAGAAGGTAGACGAGGAGGAAATTGAAATTGACCCTGACGTTGCAGCAATGATGGGATTTGGTGGATTCGGTTCATCCAAGAAGTGA

Protein Analysis

201

Amino Acids

23.34

Weight (kDa)

9.19

Isoelectric Point (pI)

56.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 83 - 107 3e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 355
AccI GTMKAC 2 cut(s) 177, 528
AccII CGCG 1 cut(s) 81
AciI CCGC 4 cut(s) 79, 346, 355, 403
AclWI GGATC 2 cut(s) 306, 455
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 47
AgsI TTSAA 7 cut(s) 59, 157, 185, 368, 389, 454, 545
AluBI AGCT 3 cut(s) 110, 329, 394
AluI AGCT 3 cut(s) 110, 329, 394
Alw21I GWGCWC 1 cut(s) 112
AlwI GGATC 2 cut(s) 306, 455
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 457, 460, 563
ApoI RAATTY 1 cut(s) 47
AspS9I GGNCC 2 cut(s) 131, 373
AvaII GGWCC 2 cut(s) 131, 373
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 112
BbvI GCAGC 3 cut(s) 469, 472, 575
BccI CCATC 1 cut(s) 565
BciVI GTATCC 1 cut(s) 239
BfuI GTATCC 1 cut(s) 239
BglII AGATCT 1 cut(s) 433
BisI GCNGC 3 cut(s) 458, 461, 564
BlsI GCNGC 3 cut(s) 459, 462, 565
Bme18I GGWCC 2 cut(s) 131, 373
BmgT120I GGNCC 2 cut(s) 131, 373
BmsI GCATC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 380
BsaWI WCCGGW 1 cut(s) 136
Bse3DI GCAATG 2 cut(s) 16, 573
BseGI GGATG 1 cut(s) 593
BseMI GCAATG 2 cut(s) 16, 573
BseMII CTCAG 2 cut(s) 60, 417
BseRI GAGGAG 2 cut(s) 116, 548
BseXI GCAGC 3 cut(s) 469, 472, 575
BsgI GTGCAG 1 cut(s) 161
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 112
BsiSI CCGG 1 cut(s) 137
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 5 cut(s) 52, 298, 412, 433, 447
BspACI CCGC 4 cut(s) 79, 346, 355, 403
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 61, 418
BspFNI CGCG 1 cut(s) 81
BspPI GGATC 2 cut(s) 306, 455
BsrBI CCGCTC 1 cut(s) 355
BsrDI GCAATG 2 cut(s) 16, 573
BssMI GATC 5 cut(s) 52, 298, 412, 433, 447
Bst6I CTCTTC 3 cut(s) 54, 93, 465
BstC8I GCNNGC 6 cut(s) 7, 79, 83, 236, 240, 357
BstDEI CTNAG 2 cut(s) 69, 426
BstF5I GGATG 1 cut(s) 593
BstFNI CGCG 1 cut(s) 81
BstKTI GATC 5 cut(s) 55, 301, 415, 436, 450
BstMBI GATC 5 cut(s) 52, 298, 412, 433, 447
BstMWI GCNNNNNNNGC 5 cut(s) 74, 148, 157, 352, 400
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 3 cut(s) 469, 472, 575
BstX2I RGATCY 2 cut(s) 433, 447
BstXI CCANNNNNNTGG 1 cut(s) 242
BstYI RGATCY 2 cut(s) 433, 447
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 593
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 6 cut(s) 7, 79, 83, 236, 240, 357
Cfr13I GGNCC 2 cut(s) 131, 373
CviJI RGCY 8 cut(s) 5, 77, 110, 151, 234, 242, 329, 394
CviKI_1 RGCY 8 cut(s) 5, 77, 110, 151, 234, 242, 329, 394
DdeI CTNAG 2 cut(s) 69, 426
DpnI GATC 5 cut(s) 54, 300, 414, 435, 449
DpnII GATC 5 cut(s) 52, 298, 412, 433, 447
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 54, 93, 465
EarI CTCTTC 3 cut(s) 54, 93, 465
Ecl136II GAGCTC 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 131, 373
Eco53kI GAGCTC 1 cut(s) 110
EcoICRI GAGCTC 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 112
FaiI YATR 6 cut(s) 39, 66, 171, 303, 338, 344
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FauI CCCGC 3 cut(s) 86, 339, 348
FblI GTMKAC 2 cut(s) 177, 528
Fnu4HI GCNGC 3 cut(s) 458, 461, 564
FokI GGATG 1 cut(s) 580
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 3 cut(s) 458, 461, 564
GluI GCNGC 3 cut(s) 458, 461, 564
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 137
HinfI GANTC 4 cut(s) 185, 281, 379, 585
HpaII CCGG 1 cut(s) 137
Hpy166II GTNNAC 2 cut(s) 178, 529
Hpy188I TCNGA 1 cut(s) 23
Hpy188III TCNNGA 7 cut(s) 56, 365, 376, 397, 437, 451, 494
Hpy8I GTNNAC 2 cut(s) 178, 529
Hpy99I CGWCG 1 cut(s) 495
HpyAV CCTTC 3 cut(s) 36, 430, 517
HpyCH4IV ACGT 2 cut(s) 490, 558
HpyCH4V TGCA 3 cut(s) 142, 287, 563
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 148, 157, 352, 400
HpyF3I CTNAG 2 cut(s) 69, 426
HpySE526I ACGT 2 cut(s) 490, 558
Kzo9I GATC 5 cut(s) 52, 298, 412, 433, 447
LmnI GCTCC 1 cut(s) 107
Lsp1109I GCAGC 3 cut(s) 469, 472, 575
LweI GCATC 1 cut(s) 368
MaeII ACGT 2 cut(s) 490, 558
MaeIII GTNAC 1 cut(s) 211
MalI GATC 5 cut(s) 54, 300, 414, 435, 449
MbiI CCGCTC 1 cut(s) 355
MboI GATC 5 cut(s) 52, 298, 412, 433, 447
MboII GAAGA 7 cut(s) 71, 110, 479, 482, 514, 523, 526
MflI RGATCY 2 cut(s) 433, 447
MhlI GDGCHC 1 cut(s) 112
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 47, 540, 546
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 388
MnlI CCTC 7 cut(s) 94, 97, 138, 144, 466, 526, 529
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 230
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 5 cut(s) 74, 148, 157, 352, 400
NdeII GATC 5 cut(s) 52, 298, 412, 433, 447
PfeI GAWTC 3 cut(s) 185, 281, 585
PflFI GACNNNGTC 1 cut(s) 489
PkrI GCNGC 3 cut(s) 459, 462, 565
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
PpuMI RGGWCCY 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 131
PspPI GGNCC 2 cut(s) 131, 373
PspPPI RGGWCCY 1 cut(s) 131
PsuI RGATCY 2 cut(s) 433, 447
PsyI GACNNNGTC 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 3 cut(s) 458, 461, 564
Sau3AI GATC 5 cut(s) 52, 298, 412, 433, 447
Sau96I GGNCC 2 cut(s) 131, 373
SchI GAGTC 1 cut(s) 388
SduI GDGCHC 1 cut(s) 112
SfaNI GCATC 1 cut(s) 368
SinI GGWCC 2 cut(s) 131, 373
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 3 cut(s) 47, 540, 546
SsiI CCGC 4 cut(s) 79, 346, 355, 403
SstI GAGCTC 1 cut(s) 112
TaiI ACGT 2 cut(s) 493, 561
TaqI TCGA 5 cut(s) 51, 119, 125, 382, 493
TasI AATT 3 cut(s) 47, 540, 546
TfiI GAWTC 3 cut(s) 185, 281, 585
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 1 cut(s) 430
TseI GCWGC 3 cut(s) 457, 460, 563
TspDTI ATGAA 3 cut(s) 186, 456, 582
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 1 cut(s) 430
Tth111I GACNNNGTC 1 cut(s) 489
VpaK11BI GGWCC 2 cut(s) 131, 373
XapI RAATTY 1 cut(s) 47
XmiI GTMKAC 2 cut(s) 177, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.