pycom04g15650

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015718
Physical Location & Seq
Forward (+)
18476666 .. 18477444
779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15650.2

Sequence Viewer

Length: 240 bp
ATGCACCGTCTCACCTCTCAAAGGCCGATCTTCCTCAAAACCGCCGTTAGAGCTTCAAACCGGAGCGTCGCGGCCAGGTCTTCTTCTCCGGGACTCTACTCCGCCAAGCAGTACGAACTCACGGTTCCCAACGTCGACTTGGTTCTAGAGGACGTCCGCCCCTATCTCATCGCCGATGGCGGTAACGTCGATGTTGTCTCCATTGAAGACGGCGTCGTTTCACTCAAGCTGGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

80

Amino Acids

8.61

Weight (kDa)

6.55

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 156
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 71
AciI CCGC 5 cut(s) 42, 71, 102, 157, 180
AcoI YGGCCR 1 cut(s) 72
AcyI GRCGYC 2 cut(s) 153, 213
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 2 cut(s) 57, 206
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 2 cut(s) 53, 229
AluI AGCT 2 cut(s) 53, 229
Alw26I GTCTC 2 cut(s) 14, 202
AoxI GGCC 2 cut(s) 23, 72
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 72, 213
BccI CCATC 1 cut(s) 170
BceAI ACGGC 2 cut(s) 29, 226
BciT130I CCWGG 1 cut(s) 76
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 14, 202
BfaI CTAG 1 cut(s) 146
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 76, 90
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 2 cut(s) 76, 90
BpiI GAAGAC 2 cut(s) 72, 213
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 1 cut(s) 90
BsaHI GRCGYC 2 cut(s) 153, 213
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 1 cut(s) 21
BseBI CCWGG 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 25, 74
BsiSI CCGG 2 cut(s) 61, 89
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 21
BsmAI GTCTC 2 cut(s) 14, 202
BsmBI CGTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 2 cut(s) 25, 74
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 5 cut(s) 42, 71, 102, 157, 180
BspANI GGCC 2 cut(s) 25, 74
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 126
BssMI GATC 1 cut(s) 27
BssNI GRCGYC 2 cut(s) 153, 213
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 2 cut(s) 8, 124
BstACI GRCGYC 2 cut(s) 153, 213
BstENI CCTNNNNNAGG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 1 cut(s) 30
BstMAI GTCTC 2 cut(s) 14, 202
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 74, 88
BstUI CGCG 1 cut(s) 71
BstV2I GAAGAC 2 cut(s) 72, 213
BsuRI GGCC 2 cut(s) 25, 74
BtgZI GCGATG 1 cut(s) 154
CseI GACGC 2 cut(s) 55, 202
Csp6I GTAC 1 cut(s) 112
CviJI RGCY 4 cut(s) 25, 53, 74, 229
CviKI_1 RGCY 4 cut(s) 25, 53, 74, 229
CviQI GTAC 1 cut(s) 112
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
EaeI YGGCCR 1 cut(s) 72
EciI GGCGGA 2 cut(s) 91, 146
EcoNI CCTNNNNNAGG 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 74
Esp3I CGTCTC 1 cut(s) 14
FaqI GGGAC 1 cut(s) 105
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 1 cut(s) 146
GluI GCNGC 1 cut(s) 72
HaeIII GGCC 2 cut(s) 25, 74
HapII CCGG 2 cut(s) 61, 89
HgaI GACGC 2 cut(s) 55, 202
Hin1I GRCGYC 2 cut(s) 153, 213
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HinfI GANTC 1 cut(s) 93
HpaII CCGG 2 cut(s) 61, 89
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 1 cut(s) 136
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 4 cut(s) 71, 137, 191, 218
HpyCH4III ACNGT 2 cut(s) 8, 124
HpyCH4IV ACGT 3 cut(s) 132, 153, 186
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpySE526I ACGT 3 cut(s) 132, 153, 186
Hsp92I GRCGYC 2 cut(s) 153, 213
Kzo9I GATC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 63
LpnPI CCDG 5 cut(s) 61, 74, 88, 102, 215
MaeI CTAG 1 cut(s) 146
MaeII ACGT 3 cut(s) 132, 153, 186
MaeIII GTNAC 1 cut(s) 182
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 4 cut(s) 22, 72, 75, 218
MlyI GAGTC 1 cut(s) 87
MnlI CCTC 3 cut(s) 25, 44, 142
MspI CCGG 2 cut(s) 61, 89
MspR9I CCNGG 2 cut(s) 76, 90
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 50
NciI CCSGG 1 cut(s) 90
NdeII GATC 1 cut(s) 27
NlaIV GGNNCC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 212
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 1 cut(s) 73
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 126
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SalI GTCGAC 1 cut(s) 134
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 1 cut(s) 27
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 2 cut(s) 76, 90
SetI ASST 7 cut(s) 17, 55, 80, 135, 156, 189, 231
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SsiI CCGC 5 cut(s) 42, 71, 102, 157, 180
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 74, 88
TaaI ACNGT 2 cut(s) 8, 124
TaiI ACGT 3 cut(s) 135, 156, 189
TaqI TCGA 2 cut(s) 135, 189
TauI GCSGC 1 cut(s) 74
Tth111I GACNNNGTC 1 cut(s) 212
XagI CCTNNNNNAGG 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 145
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 135
XspI CTAG 1 cut(s) 146
ZraI GACGTC 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.