Rw1G007500

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015718
Physical Location & Seq
Forward (+)
15814605 .. 15816546
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G007500.1

Sequence Viewer

Length: 633 bp
ATGGTTCCTTCTGATTTCTTCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGTGAAATCCCATCAATCCCTACAACTTATGCTTGTAAAACGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTTTTTTCCGCCATTAGAGCTTCGAACGCAAGCGCTGCCTCCGAGTCTTCTTCTTCTTCTCCGGGACTCTACTCGGCCAAGCAATTTGAGCTCATAGTTCCCAACATTGACTTGGTTCTCAAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGTGTCGTTTCTCTCAAGCTCCAAGTGTTTAAAGAAAAGTTTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAGAGACCACAGTTGAGTATATTGATTGTTCATTAGATGGTCATGGATTTACATGCCCTTTCTTTTCCATGCTGGTGACCAAGCAGAGCAGTGGAGATTTTGGGGCTAATGTGAAGCGCCTTGAAGTGGAAACTGAGGAAAAGATCAATCACCTCTCAACAGAGGGCTCAAGGATATCAGGTGATGTTGTCCAGATGCTTCTAAAGCATGTGATGACAATGAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

210

Amino Acids

23.36

Weight (kDa)

5.6

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 294
AciI CCGC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 258
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 529
AgsI TTSAA 2 cut(s) 380, 527
AhdI GACNNNNNGTC 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 3 cut(s) 152, 223, 334
AluI AGCT 3 cut(s) 152, 223, 334
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 401
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 2 cut(s) 207, 262
ApeKI GCWGC 1 cut(s) 167
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 2 cut(s) 167, 522
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 3 cut(s) 490, 545, 596
AsuII TTCGAA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 225, 572
BbsI GAAGAC 2 cut(s) 171, 318
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 154
BccI CCATC 3 cut(s) 78, 275, 434
BceAI ACGGC 1 cut(s) 90
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 401
BfoI RGCGCY 2 cut(s) 168, 523
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
Bme1390I CCNGG 1 cut(s) 195
BmeRI GACNNNNNGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 3 cut(s) 6, 104, 265
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 2 cut(s) 352, 588
BmuI ACTGGG 1 cut(s) 53
BpiI GAAGAC 2 cut(s) 171, 318
BplI GAGNNNNNCTC 2 cut(s) 351, 383
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 4 cut(s) 236, 314, 353, 556
BpuMI CCSGG 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 401
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 154
BshFI GGCC 2 cut(s) 209, 264
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 210
BsnI GGCC 2 cut(s) 209, 264
Bso31I GGTCTC 1 cut(s) 401
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 225, 572
Bsp143I GATC 1 cut(s) 546
BspACI CCGC 1 cut(s) 141
BspANI GGCC 2 cut(s) 209, 264
BspCNI CTCAG 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 6, 104, 265
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 401
BsrI ACTGG 1 cut(s) 59
BssMI GATC 1 cut(s) 546
BssNI GRCGYC 1 cut(s) 258
Bst4CI ACNGT 3 cut(s) 102, 317, 415
BstACI GRCGYC 1 cut(s) 258
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 537
BstEII GGTNACC 1 cut(s) 478
BstH2I RGCGCY 2 cut(s) 168, 523
BstHHI GCGC 2 cut(s) 167, 522
BstKTI GATC 1 cut(s) 549
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 1 cut(s) 546
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 607
BstNSI RCATGY 2 cut(s) 459, 614
BstPI GGTNACC 1 cut(s) 478
BstSCI CCNGG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 2 cut(s) 171, 318
BsuRI GGCC 2 cut(s) 209, 264
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 2 cut(s) 66, 499
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 2 cut(s) 167, 522
Cfr13I GGNCC 1 cut(s) 263
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 4 cut(s) 446, 456, 472, 611
CviJI RGCY 7 cut(s) 152, 209, 223, 264, 334, 509, 570
CviKI_1 RGCY 7 cut(s) 152, 209, 223, 264, 334, 509, 570
DdeI CTNAG 1 cut(s) 537
DpnI GATC 1 cut(s) 548
DpnII GATC 1 cut(s) 546
DraI TTTAAA 1 cut(s) 346
DriI GACNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 301
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 225, 572
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 579
Eco47III AGCGCT 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 223
Eco91I GGTNACC 1 cut(s) 478
EcoICRI GAGCTC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 478
EcoRV GATATC 1 cut(s) 579
EcoT38I GRGCYC 2 cut(s) 225, 572
FaeI CATG 4 cut(s) 449, 459, 475, 614
FaiI YATR 7 cut(s) 89, 227, 423, 447, 457, 473, 612
FaqI GGGAC 1 cut(s) 210
FatI CATG 4 cut(s) 445, 455, 471, 610
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 168
FriOI GRGCYC 2 cut(s) 225, 572
Fsp4HI GCNGC 1 cut(s) 168
GlaI GCGC 2 cut(s) 166, 521
GluI GCNGC 1 cut(s) 168
HaeII RGCGCY 2 cut(s) 168, 523
HaeIII GGCC 2 cut(s) 209, 264
HapII CCGG 1 cut(s) 194
HhaI GCGC 2 cut(s) 167, 522
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 4 cut(s) 449, 459, 475, 614
Hin6I GCGC 2 cut(s) 165, 520
HinP1I GCGC 2 cut(s) 165, 520
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 126, 176, 198
HpaII CCGG 1 cut(s) 194
HphI GGTGA 3 cut(s) 490, 545, 596
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 13, 175
Hpy188III TCNNGA 1 cut(s) 595
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 4 cut(s) 263, 296, 299, 311
HpyAV CCTTC 2 cut(s) 18, 54
HpyCH4III ACNGT 3 cut(s) 102, 317, 415
HpyCH4IV ACGT 3 cut(s) 258, 291, 297
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 607
HpyF3I CTNAG 1 cut(s) 537
HpySE526I ACGT 3 cut(s) 258, 291, 297
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 4 cut(s) 449, 459, 475, 614
HspAI GCGC 2 cut(s) 165, 520
Kzo9I GATC 1 cut(s) 546
LmnI GCTCC 1 cut(s) 339
LpnPI CCDG 6 cut(s) 7, 72, 207, 461, 567, 608
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 2 cut(s) 352, 588
MaeII ACGT 3 cut(s) 258, 291, 297
MaeIII GTNAC 1 cut(s) 478
MalI GATC 1 cut(s) 548
MboI GATC 1 cut(s) 546
MboII GAAGA 9 cut(s) 10, 39, 135, 171, 174, 177, 180, 323, 407
MhlI GDGCHC 2 cut(s) 225, 572
MluCI AATT 3 cut(s) 39, 215, 628
MlyI GAGTC 2 cut(s) 185, 192
MnlI CCTC 5 cut(s) 126, 181, 532, 559, 566
MseI TTAA 2 cut(s) 345, 631
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 220, 607
NciI CCSGG 1 cut(s) 195
NdeII GATC 1 cut(s) 546
NlaIII CATG 4 cut(s) 449, 459, 475, 614
NlaIV GGNNCC 3 cut(s) 6, 104, 265
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 478
NspI RCATGY 2 cut(s) 459, 614
NspV TTCGAA 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 317
PfoI TCCNGGA 1 cut(s) 193
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 2 cut(s) 184, 192
PpsI GAGTC 2 cut(s) 184, 192
Psp124BI GAGCTC 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 478
PspN4I GGNNCC 3 cut(s) 6, 104, 265
PspPI GGNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 345, 631
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 1 cut(s) 546
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 185, 192
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 2 cut(s) 225, 572
SetI ASST 8 cut(s) 154, 225, 261, 294, 300, 336, 558, 586
SfaNI GCATC 2 cut(s) 352, 588
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 4 cut(s) 251, 329, 368, 571
SmoI CTYRAG 4 cut(s) 251, 329, 368, 571
Sse9I AATT 3 cut(s) 39, 215, 628
SsiI CCGC 1 cut(s) 141
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 3 cut(s) 102, 317, 415
TaiI ACGT 3 cut(s) 261, 294, 300
TaqI TCGA 3 cut(s) 155, 294, 309
TasI AATT 3 cut(s) 39, 215, 628
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 2 cut(s) 345, 631
Tru9I TTAA 2 cut(s) 345, 631
TscAI CASTG 2 cut(s) 66, 499
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 408, 423
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 2 cut(s) 66, 499
Tth111I GACNNNGTC 1 cut(s) 317
XceI RCATGY 2 cut(s) 459, 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmiI GTMKAC 1 cut(s) 294
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.