RLG00000007734

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015718
Physical Location & Seq
Forward (+)
20430742 .. 20433813
3072 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007734

Sequence Viewer

Length: 567 bp
ATGCTTGTAAAACGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCGCCATTAGAGCTTCGAATGCAAGTACTGCCGCCGAGTCTTCTTCTTCTTCTTCTCCGGGACTCTACTCAGCCAAGCAATTTGAGCTCACAGTTCCCAACATTGACTTGGTTCTCGAGGATGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGATGGCGTCTTTCTCTCAAGCTCCAAGTATATTGATTGTTCATTAGATGGTCATGGATTTACATGCCCTTTCTTTTCCTTGCTGGTGACCAAGCAGAGCAGTGGAGATTTTGGGGCTAATGTGAAGCGCCTTGAAGTGGAAACTGAGGAAAAGATCAATCACCTCTCAACAGAGGGCTCAAGGATATCAGGTGATGTTGTCCAGATGCTTCTGAAGCATGTGACGATAGCAACATCTGAAACTGGTTCACCTGCGGATGAAACTGAATTTGAGAAGTTAGAAGAGAAAGCCTTCAATTTGAGAAAGATCAAGAGAAGGACCTGGTTGGACGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

189

Amino Acids

20.64

Weight (kDa)

5.15

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 487
Acc36I ACCTGC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 210
AciI CCGC 3 cut(s) 54, 84, 482
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 461
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 2 cut(s) 362, 523
AhdI GACNNNNNGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 548
AluBI AGCT 3 cut(s) 65, 139, 249
AluI AGCT 3 cut(s) 65, 139, 249
Alw21I GWGCWC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 1 cut(s) 178
ApoI RAATTY 1 cut(s) 494
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 2 cut(s) 179, 546
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 4 cut(s) 325, 380, 431, 468
AsuII TTCGAA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 546
BanI GGYRCC 1 cut(s) 15
BanII GRGCYC 2 cut(s) 141, 407
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 141
BccI CCATC 3 cut(s) 191, 224, 269
BceAI ACGGC 1 cut(s) 3
BciT130I CCWGG 1 cut(s) 550
BcnI CCSGG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 487
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 2 cut(s) 111, 550
Bme18I GGWCC 1 cut(s) 546
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 179, 546
BmiI GGNNCC 2 cut(s) 17, 181
BmrFI CCNGG 2 cut(s) 111, 550
BmsI GCATC 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 229, 391
BpuMI CCSGG 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 234
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 475
BseBI CCWGG 1 cut(s) 550
BseGI GGATG 2 cut(s) 178, 490
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMII CTCAG 2 cut(s) 135, 363
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 1 cut(s) 18
BshFI GGCC 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 1 cut(s) 110
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 76
BsnI GGCC 1 cut(s) 180
BsoBI CYCGRG 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 2 cut(s) 141, 407
Bsp143I GATC 2 cut(s) 381, 534
BspACI CCGC 3 cut(s) 54, 84, 482
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 2 cut(s) 134, 364
BspHI TCATGA 1 cut(s) 563
BspLI GGNNCC 2 cut(s) 17, 181
BspMI ACCTGC 1 cut(s) 487
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 15
BsrI ACTGG 1 cut(s) 475
BssMI GATC 2 cut(s) 381, 534
BssNI GRCGYC 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 550
Bst4CI ACNGT 2 cut(s) 15, 145
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 68
BstDEI CTNAG 2 cut(s) 121, 372
BstEII GGTNACC 1 cut(s) 313
BstF5I GGATG 2 cut(s) 178, 490
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 2 cut(s) 384, 537
BstMBI GATC 2 cut(s) 381, 534
BstMWI GCNNNNNNNGC 5 cut(s) 62, 71, 80, 136, 442
BstNI CCWGG 1 cut(s) 550
BstNSI RCATGY 2 cut(s) 294, 449
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 2 cut(s) 109, 548
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 180
BtsCI GGATG 2 cut(s) 178, 490
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 487
CciI TCATGA 1 cut(s) 563
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 2 cut(s) 179, 546
CseI GACGC 1 cut(s) 223
CsiI ACCWGGT 1 cut(s) 548
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 4 cut(s) 281, 291, 446, 564
CviJI RGCY 8 cut(s) 65, 125, 139, 180, 249, 344, 405, 518
CviKI_1 RGCY 8 cut(s) 65, 125, 139, 180, 249, 344, 405, 518
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 2 cut(s) 121, 372
DpnI GATC 2 cut(s) 383, 536
DpnII GATC 2 cut(s) 381, 534
DriI GACNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 504
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 504
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 139
Eco24I GRGCYC 2 cut(s) 141, 407
Eco32I GATATC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 546
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 546
EcoO65I GGTNACC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 548
EcoRV GATATC 1 cut(s) 414
EcoT38I GRGCYC 2 cut(s) 141, 407
FaeI CATG 4 cut(s) 284, 294, 449, 567
FaiI YATR 5 cut(s) 258, 282, 292, 447, 565
FaqI GGGAC 1 cut(s) 126
FatI CATG 4 cut(s) 280, 290, 445, 563
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 2 cut(s) 185, 497
FriOI GRGCYC 2 cut(s) 141, 407
Fsp4HI GCNGC 1 cut(s) 84
GlaI GCGC 1 cut(s) 356
GluI GCNGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 358
HaeIII GGCC 1 cut(s) 180
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 284, 294, 449, 567
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 4 cut(s) 39, 89, 114, 560
HpaII CCGG 1 cut(s) 110
HphI GGTGA 4 cut(s) 325, 380, 431, 468
Hpy166II GTNNAC 2 cut(s) 211, 476
Hpy188I TCNGA 2 cut(s) 441, 466
Hpy188III TCNNGA 4 cut(s) 167, 430, 538, 564
Hpy8I GTNNAC 2 cut(s) 211, 476
Hpy99I CGWCG 3 cut(s) 212, 215, 227
HpyAV CCTTC 2 cut(s) 529, 537
HpyCH4III ACNGT 2 cut(s) 15, 145
HpyCH4IV ACGT 2 cut(s) 207, 213
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 71, 80, 136, 442
HpyF3I CTNAG 2 cut(s) 121, 372
HpySE526I ACGT 2 cut(s) 207, 213
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 284, 294, 449, 567
HspAI GCGC 1 cut(s) 355
Kzo9I GATC 2 cut(s) 381, 534
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 8 cut(s) 123, 296, 402, 443, 456, 492, 535, 562
LweI GCATC 1 cut(s) 423
MabI ACCWGGT 1 cut(s) 548
MaeII ACGT 2 cut(s) 207, 213
MaeIII GTNAC 2 cut(s) 313, 448
MalI GATC 2 cut(s) 383, 536
MboI GATC 2 cut(s) 381, 534
MboII GAAGA 8 cut(s) 48, 84, 87, 90, 93, 96, 239, 521
MhlI GDGCHC 2 cut(s) 141, 407
MluCI AATT 3 cut(s) 131, 494, 523
MlyI GAGTC 2 cut(s) 98, 108
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 5 cut(s) 39, 163, 367, 394, 401
MroXI GAANNNNTTC 1 cut(s) 518
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 2 cut(s) 111, 550
Mva1269I GAATGC 1 cut(s) 76
MvaI CCWGG 1 cut(s) 550
MwoI GCNNNNNNNGC 5 cut(s) 62, 71, 80, 136, 442
NciI CCSGG 1 cut(s) 111
NdeII GATC 2 cut(s) 381, 534
NlaIII CATG 4 cut(s) 284, 294, 449, 567
NlaIV GGNNCC 2 cut(s) 17, 181
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 313, 448
NspI RCATGY 2 cut(s) 294, 449
NspV TTCGAA 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 167
PagI TCATGA 1 cut(s) 563
PaqCI CACCTGC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 231
PctI GAATGC 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 39, 560
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 2 cut(s) 97, 108
PpsI GAGTC 2 cut(s) 97, 108
PpuMI RGGWCCY 1 cut(s) 546
Psp124BI GAGCTC 1 cut(s) 141
Psp5II RGGWCCY 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 548
PspEI GGTNACC 1 cut(s) 313
PspGI CCWGG 1 cut(s) 548
PspN4I GGNNCC 2 cut(s) 17, 181
PspPI GGNCC 2 cut(s) 179, 546
PspPPI RGGWCCY 1 cut(s) 546
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SacI GAGCTC 1 cut(s) 141
SalI GTCGAC 1 cut(s) 209
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 381, 534
Sau96I GGNCC 2 cut(s) 179, 546
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 2 cut(s) 98, 108
ScrFI CCNGG 2 cut(s) 111, 550
SduI GDGCHC 2 cut(s) 141, 407
SetI ASST 9 cut(s) 67, 141, 210, 216, 251, 393, 421, 481, 551
SexAI ACCWGGT 1 cut(s) 548
SfaNI GCATC 1 cut(s) 423
Sfr274I CTCGAG 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 68
SgrDI CGTCGACG 1 cut(s) 209
SinI GGWCC 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 3 cut(s) 167, 244, 406
SmoI CTYRAG 3 cut(s) 167, 244, 406
Sse9I AATT 3 cut(s) 131, 494, 523
SsiI CCGC 3 cut(s) 54, 84, 482
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 109, 548
TaaI ACNGT 2 cut(s) 15, 145
TaiI ACGT 2 cut(s) 210, 216
TaqI TCGA 4 cut(s) 68, 168, 210, 225
TasI AATT 3 cut(s) 131, 494, 523
TatI WGTACW 1 cut(s) 77
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 39, 560
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 313, 448
Tsp45I GTSAC 2 cut(s) 313, 448
TspDTI ATGAA 3 cut(s) 258, 501, 552
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 546
XapI RAATTY 1 cut(s) 494
XceI RCATGY 2 cut(s) 294, 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 518
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.