RLG00000013906

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015718
Physical Location & Seq
Forward (+)
44068600 .. 44070532
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013906

Sequence Viewer

Length: 537 bp
ATGGTTCCTTCTGATTTCTCCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCGAAATCCCATCAATCCCCACAACTTATGCTTGTAAAATGGTGCCGTCTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCGCCATTAGAGCTTCGAACGCAAGTACTGCCGCCGAGTCTTCTTCTTCTTCTTCTTCGGGACTCTACTCAGCCAAGCAATTTGAGCTCACAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCGGCCCCTATCTCATCAACGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCTTTCTCTCAAGCTCCAAGTATATTGATTGTTCATTAGATGGTCATGGATTTACATGCCCTTTCTTTTCCTTGCTGGTGACCAAGCAGAGCAGTGGAGATTTTGGGGCTAATGTGAAGCGCCTTGAAGTGGAAACTGAGGAAAAGATCAATCACCTCTCAACAGAGGGCTCAAGGATATCAGGTGATGTTGTCCAGATGCTTCTGAGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

179

Amino Acids

19.31

Weight (kDa)

5.12

Isoelectric Point (pI)

54.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 297
AciI CCGC 2 cut(s) 141, 171
AcyI GRCGYC 2 cut(s) 261, 321
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 1 cut(s) 449
AhdI GACNNNNNGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 20
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 3 cut(s) 152, 226, 336
AluI AGCT 3 cut(s) 152, 226, 336
Alw21I GWGCWC 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 1 cut(s) 265
ArsI GACNNNNNNTTYG 2 cut(s) 306, 338
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 412, 467, 518
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 254
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 228, 494
BbsI GAAGAC 2 cut(s) 171, 321
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 3 cut(s) 78, 278, 356
BceAI ACGGC 2 cut(s) 90, 334
BciT130I CCWGG 1 cut(s) 22
BfoI RGCGCY 1 cut(s) 445
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 22
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 3 cut(s) 6, 104, 268
BmrFI CCNGG 1 cut(s) 22
BmsI GCATC 1 cut(s) 510
BpiI GAAGAC 2 cut(s) 171, 321
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 316, 478
BsaHI GRCGYC 2 cut(s) 261, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMII CTCAG 3 cut(s) 222, 450, 518
BseRI GAGGAG 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 228, 494
Bsp143I GATC 1 cut(s) 468
BspACI CCGC 2 cut(s) 141, 171
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 3 cut(s) 221, 451, 519
BspLI GGNNCC 3 cut(s) 6, 104, 268
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BssMI GATC 1 cut(s) 468
BssNI GRCGYC 2 cut(s) 261, 321
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 232
BstACI GRCGYC 2 cut(s) 261, 321
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstDEI CTNAG 3 cut(s) 208, 459, 527
BstEII GGTNACC 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 445
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 1 cut(s) 471
BstMBI GATC 1 cut(s) 468
BstMWI GCNNNNNNNGC 4 cut(s) 149, 158, 167, 223
BstNI CCWGG 1 cut(s) 22
BstNSI RCATGY 2 cut(s) 381, 535
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 20
BstV2I GAAGAC 2 cut(s) 171, 321
BsuRI GGCC 1 cut(s) 267
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 421
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 1 cut(s) 266
CseI GACGC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 165
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 3 cut(s) 368, 378, 532
CviJI RGCY 7 cut(s) 152, 212, 226, 267, 336, 431, 492
CviKI_1 RGCY 7 cut(s) 152, 212, 226, 267, 336, 431, 492
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 3 cut(s) 208, 459, 527
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
DriI GACNNNNNGTC 1 cut(s) 304
Eam1105I GACNNNNNGTC 1 cut(s) 304
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 226
Eco24I GRGCYC 2 cut(s) 228, 494
Eco32I GATATC 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 20
EcoRV GATATC 1 cut(s) 501
EcoT38I GRGCYC 2 cut(s) 228, 494
FaeI CATG 3 cut(s) 371, 381, 535
FaiI YATR 5 cut(s) 89, 345, 369, 379, 533
FaqI GGGAC 1 cut(s) 213
FatI CATG 3 cut(s) 367, 377, 531
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 171
FriOI GRGCYC 2 cut(s) 228, 494
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 1 cut(s) 443
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 445
HaeIII GGCC 1 cut(s) 267
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 2 cut(s) 261, 321
Hin1II CATG 3 cut(s) 371, 381, 535
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 3 cut(s) 126, 176, 201
HphI GGTGA 3 cut(s) 412, 467, 518
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 13, 528
Hpy188III TCNNGA 3 cut(s) 198, 254, 517
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 4 cut(s) 266, 299, 302, 314
HpyAV CCTTC 2 cut(s) 18, 54
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 3 cut(s) 261, 294, 300
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 158, 167, 223
HpyF3I CTNAG 3 cut(s) 208, 459, 527
HpySE526I ACGT 3 cut(s) 261, 294, 300
Hsp92I GRCGYC 2 cut(s) 261, 321
Hsp92II CATG 3 cut(s) 371, 381, 535
HspAI GCGC 1 cut(s) 442
Kzo9I GATC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 341
LpnPI CCDG 6 cut(s) 7, 34, 72, 383, 489, 530
LweI GCATC 1 cut(s) 510
MaeII ACGT 3 cut(s) 261, 294, 300
MaeIII GTNAC 1 cut(s) 400
MalI GATC 1 cut(s) 470
MboI GATC 1 cut(s) 468
MboII GAAGA 9 cut(s) 39, 135, 171, 174, 177, 180, 183, 186, 326
MhlI GDGCHC 2 cut(s) 228, 494
MluCI AATT 2 cut(s) 39, 218
MlyI GAGTC 2 cut(s) 185, 195
MnlI CCTC 5 cut(s) 126, 250, 454, 481, 488
MspR9I CCNGG 1 cut(s) 22
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 4 cut(s) 149, 158, 167, 223
NdeII GATC 1 cut(s) 468
NlaIII CATG 3 cut(s) 371, 381, 535
NlaIV GGNNCC 3 cut(s) 6, 104, 268
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 2 cut(s) 381, 535
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 318
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 320
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 2 cut(s) 184, 195
PpsI GAGTC 2 cut(s) 184, 195
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 20
PspEI GGTNACC 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 3 cut(s) 6, 104, 268
PspPI GGNCC 1 cut(s) 266
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 296
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 468
Sau96I GGNCC 1 cut(s) 266
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 2 cut(s) 185, 195
ScrFI CCNGG 1 cut(s) 22
SduI GDGCHC 2 cut(s) 228, 494
SetI ASST 8 cut(s) 154, 228, 264, 297, 303, 338, 480, 508
SfaNI GCATC 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 254
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 296
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 3 cut(s) 254, 331, 493
SmoI CTYRAG 3 cut(s) 254, 331, 493
Sse9I AATT 2 cut(s) 39, 218
SsiI CCGC 2 cut(s) 141, 171
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 3 cut(s) 264, 297, 303
TaqI TCGA 4 cut(s) 155, 255, 297, 312
TasI AATT 2 cut(s) 39, 218
TatI WGTACW 1 cut(s) 164
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 126
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 1 cut(s) 345
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 320
XceI RCATGY 2 cut(s) 381, 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 297
ZraI GACGTC 1 cut(s) 262
ZrmI AGTACT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.