RLG00000036894

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015718
Physical Location & Seq
Reverse (-)
86206069 .. 86209142
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036894

Sequence Viewer

Length: 654 bp
ATGGTTCCTTCTGATTTCTCCTGGTTGCCCAAAACCCCAATTCCTTCTTCTCAAATCCCAGCAAAATCCCATCAATCCCCACAACTTATGCTTGTAAAACGGTACCGTCTAACTCCTCAAAGAAGAATCTTTCTCTGTTCCGCCATTAGAGCTTCGAACGCAAGTACTGCCGCCGAGTCTTCTTCTTCTTCTTCTCCGGGACTCTACTCAGTCAAGCAATTTGAGCTCACAGTTCCCAACATTGACTTGGTTCTCGAGGACGTCGGCCCCTATCTCATCAATGATGGTGGAAACGTCGACGTTGTGTCCGTCGAAGACGGCGTCTTTCTCTCAAGCTCCAAGTATATTGATTTTTCATTAGATGGTCATGGATTTACATGCCCTTTCTTTTCCTTGCTGGTGACCAAGCAGAGCAGTGGAGATTTTGGGGCTAATGTGAAGCGCCTTGAAGTGGAAACTGAGGAAAAGATCAATCACCTCTCAACAGAGGGCTCAAGGATATCAGGTGATGTTGTCCAGATGCTTCTGAAGCATGTGACGACAGCAACATCTGAAACTGGTTCACCTGCGGATGAAACTGAATTTGAGAAGTTAGAAGAGAAAGCCTTCAATTTGAGAAAGATCAAGAGAAGGACCTGGTTGGACGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

218

Amino Acids

23.95

Weight (kDa)

5.44

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 574
AatII GACGTC 1 cut(s) 264
Acc36I ACCTGC 1 cut(s) 574
Acc65I GGTACC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 297
AciI CCGC 3 cut(s) 141, 171, 569
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 1 cut(s) 548
AcyI GRCGYC 2 cut(s) 261, 321
AfaI GTAC 2 cut(s) 104, 166
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 2 cut(s) 449, 610
AhdI GACNNNNNGTC 1 cut(s) 304
AjnI CCWGG 2 cut(s) 20, 635
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 3 cut(s) 152, 226, 336
AluI AGCT 3 cut(s) 152, 226, 336
Alw21I GWGCWC 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 1 cut(s) 265
ApoI RAATTY 1 cut(s) 581
ArsI GACNNNNNNTTYG 2 cut(s) 306, 338
Asp700I GAANNNNTTC 1 cut(s) 605
Asp718I GGTACC 1 cut(s) 102
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 2 cut(s) 266, 633
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 4 cut(s) 412, 467, 518, 555
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 633
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 228, 494
BbsI GAAGAC 2 cut(s) 171, 321
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 3 cut(s) 78, 278, 356
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 2 cut(s) 22, 637
BcnI CCSGG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 574
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 3 cut(s) 22, 198, 637
Bme18I GGWCC 1 cut(s) 633
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 2 cut(s) 266, 633
BmiI GGNNCC 3 cut(s) 6, 104, 268
BmrFI CCNGG 3 cut(s) 22, 198, 637
BmsI GCATC 1 cut(s) 510
BpiI GAAGAC 2 cut(s) 171, 321
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 316, 478
BpuMI CCSGG 1 cut(s) 198
BsaHI GRCGYC 2 cut(s) 261, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse1I ACTGG 1 cut(s) 562
BseBI CCWGG 2 cut(s) 22, 637
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMII CTCAG 2 cut(s) 222, 450
BseNI ACTGG 1 cut(s) 562
BseRI GAGGAG 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 58
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 1 cut(s) 197
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 228, 494
Bsp143I GATC 2 cut(s) 468, 621
BspACI CCGC 3 cut(s) 141, 171, 569
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 2 cut(s) 221, 451
BspHI TCATGA 1 cut(s) 650
BspLI GGNNCC 3 cut(s) 6, 104, 268
BspMI ACCTGC 1 cut(s) 574
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 102
BsrI ACTGG 1 cut(s) 562
BssMI GATC 2 cut(s) 468, 621
BssNI GRCGYC 2 cut(s) 261, 321
Bst2UI CCWGG 2 cut(s) 22, 637
Bst4CI ACNGT 3 cut(s) 102, 107, 232
Bst6I CTCTTC 1 cut(s) 591
BstACI GRCGYC 2 cut(s) 261, 321
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstDEI CTNAG 2 cut(s) 208, 459
BstEII GGTNACC 1 cut(s) 400
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 445
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 2 cut(s) 471, 624
BstMBI GATC 2 cut(s) 468, 621
BstMWI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 223, 529
BstNI CCWGG 2 cut(s) 22, 637
BstNSI RCATGY 2 cut(s) 381, 536
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 3 cut(s) 20, 196, 635
BstV2I GAAGAC 2 cut(s) 171, 321
BsuRI GGCC 1 cut(s) 267
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 421
BveI ACCTGC 1 cut(s) 574
CciI TCATGA 1 cut(s) 650
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 2 cut(s) 266, 633
CseI GACGC 1 cut(s) 310
CsiI ACCWGGT 1 cut(s) 635
Csp6I GTAC 2 cut(s) 103, 165
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 4 cut(s) 368, 378, 533, 651
CviJI RGCY 7 cut(s) 152, 226, 267, 336, 431, 492, 605
CviKI_1 RGCY 7 cut(s) 152, 226, 267, 336, 431, 492, 605
CviQI GTAC 2 cut(s) 103, 165
DdeI CTNAG 2 cut(s) 208, 459
DpnI GATC 2 cut(s) 470, 623
DpnII GATC 2 cut(s) 468, 621
DriI GACNNNNNGTC 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 591
Eam1105I GACNNNNNGTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 591
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 226
Eco24I GRGCYC 2 cut(s) 228, 494
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 633
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 633
EcoO65I GGTNACC 1 cut(s) 400
EcoRII CCWGG 2 cut(s) 20, 635
EcoRV GATATC 1 cut(s) 501
EcoT38I GRGCYC 2 cut(s) 228, 494
FaeI CATG 4 cut(s) 371, 381, 536, 654
FaiI YATR 6 cut(s) 89, 345, 369, 379, 534, 652
FaqI GGGAC 1 cut(s) 213
FatI CATG 4 cut(s) 367, 377, 532, 650
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 2 cut(s) 228, 494
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 1 cut(s) 443
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 445
HaeIII GGCC 1 cut(s) 267
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 2 cut(s) 261, 321
Hin1II CATG 4 cut(s) 371, 381, 536, 654
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 4 cut(s) 126, 176, 201, 647
HpaII CCGG 1 cut(s) 197
HphI GGTGA 4 cut(s) 412, 467, 518, 555
Hpy166II GTNNAC 2 cut(s) 298, 563
Hpy188I TCNGA 3 cut(s) 13, 528, 553
Hpy188III TCNNGA 4 cut(s) 254, 517, 625, 651
Hpy8I GTNNAC 2 cut(s) 298, 563
Hpy99I CGWCG 4 cut(s) 266, 299, 302, 314
HpyAV CCTTC 4 cut(s) 18, 54, 616, 624
HpyCH4III ACNGT 3 cut(s) 102, 107, 232
HpyCH4IV ACGT 3 cut(s) 261, 294, 300
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 223, 529
HpyF3I CTNAG 2 cut(s) 208, 459
HpySE526I ACGT 3 cut(s) 261, 294, 300
Hsp92I GRCGYC 2 cut(s) 261, 321
Hsp92II CATG 4 cut(s) 371, 381, 536, 654
HspAI GCGC 1 cut(s) 442
KpnI GGTACC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 468, 621
LmnI GCTCC 1 cut(s) 341
LweI GCATC 1 cut(s) 510
MabI ACCWGGT 1 cut(s) 635
MaeII ACGT 3 cut(s) 261, 294, 300
MaeIII GTNAC 2 cut(s) 400, 535
MalI GATC 2 cut(s) 470, 623
MboI GATC 2 cut(s) 468, 621
MboII GAAGA 9 cut(s) 39, 135, 171, 174, 177, 180, 183, 326, 608
MhlI GDGCHC 2 cut(s) 228, 494
MluCI AATT 4 cut(s) 39, 218, 581, 610
MlyI GAGTC 2 cut(s) 185, 195
MmeI TCCRAC 1 cut(s) 621
MnlI CCTC 5 cut(s) 126, 250, 454, 481, 488
MroXI GAANNNNTTC 1 cut(s) 605
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 3 cut(s) 22, 198, 637
MvaI CCWGG 2 cut(s) 22, 637
MwoI GCNNNNNNNGC 5 cut(s) 149, 158, 167, 223, 529
NciI CCSGG 1 cut(s) 198
NdeII GATC 2 cut(s) 468, 621
NlaIII CATG 4 cut(s) 371, 381, 536, 654
NlaIV GGNNCC 3 cut(s) 6, 104, 268
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 2 cut(s) 400, 535
NspI RCATGY 2 cut(s) 381, 536
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 254
PagI TCATGA 1 cut(s) 650
PaqCI CACCTGC 1 cut(s) 574
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 318
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 126, 647
PflFI GACNNNGTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 2 cut(s) 184, 195
PpsI GAGTC 2 cut(s) 184, 195
PpuMI RGGWCCY 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 20, 635
PspEI GGTNACC 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 2 cut(s) 20, 635
PspN4I GGNNCC 3 cut(s) 6, 104, 268
PspPI GGNCC 2 cut(s) 266, 633
PspPPI RGGWCCY 1 cut(s) 633
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 2 cut(s) 104, 166
RsaNI GTAC 2 cut(s) 103, 165
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 296
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 468, 621
Sau96I GGNCC 2 cut(s) 266, 633
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 2 cut(s) 185, 195
ScrFI CCNGG 3 cut(s) 22, 198, 637
SduI GDGCHC 2 cut(s) 228, 494
SexAI ACCWGGT 1 cut(s) 635
SfaNI GCATC 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 254
SfuI TTCGAA 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 296
SinI GGWCC 1 cut(s) 633
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 3 cut(s) 254, 331, 493
SmoI CTYRAG 3 cut(s) 254, 331, 493
Sse9I AATT 4 cut(s) 39, 218, 581, 610
SsiI CCGC 3 cut(s) 141, 171, 569
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 3 cut(s) 20, 196, 635
TaaI ACNGT 3 cut(s) 102, 107, 232
TaiI ACGT 3 cut(s) 264, 297, 303
TaqI TCGA 4 cut(s) 155, 255, 297, 312
TasI AATT 4 cut(s) 39, 218, 581, 610
TatI WGTACW 1 cut(s) 164
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 2 cut(s) 126, 647
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 2 cut(s) 400, 535
Tsp45I GTSAC 2 cut(s) 400, 535
TspDTI ATGAA 3 cut(s) 345, 588, 639
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 633
XapI RAATTY 1 cut(s) 581
XceI RCATGY 2 cut(s) 381, 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 605
ZraI GACGTC 1 cut(s) 262
ZrmI AGTACT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.