pycom04g21580

Isopentenyl-diphosphate Delta-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0009079
Physical Location & Seq
Reverse (-)
22674693 .. 22677446
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGAGTCCCACCACTACTCGCCTTCTCCACACAATCTCCTTCTTCCCCAAATCTCCTCCTCCTCTCTTCTCATCCGCTGCGACTCGAATCTCTTCCTTCTCCAAGCCCTCCGCATTCCCCTCCCTCTCCGCCAGGCTATCCCTCTCTTTTCACATCTCCACCATGGCCGACGCTCCCGACGCCGGCATGGACGCCGTCCAGCGCCGCCTCATGTTCGAAGACGAATGTATTCTGGTGGATGAGAATGATCGTGTTGTAGGTCACGACACCAAGTACAATTGTCACTTGATGGAAAAGATCGATCAACAATCTGAAAATTTGCTTCACAGAGCTTTCAGTGTCTTTTTGTTTAACTCAAAGCATGAGTTGCTTCTTCAGCAACGTTCTGCAACCAAGGTAACATTTCCACTTGTGTGGACAAACACCTGTTGCAGCCATCCGCTGTACCGTGAATCCGAGCTTATTGATGAGAACTCTCTTGGGGTAAGAAACGCTGCTCAAAGGAAGCTTTTGGATGAACTCGGTATTCCTGCTGAAGATGTGCCAGTTGATCAGTTTATCCCGTTGGGTCGCATGCTGTACAAGGCACCTTCTGATGGCAAGTGGGGAGAACATGAACTTGATTACCTTCTCTTCACTGTACGAGATGTTAATGTGCATCCGAACCCTGATGAGGTAGCTGATATCAAGTACGTAAACAAGGAGCAGCTGAAAGAGCTGTTGAGGAAGGCAGATGCCGGGGAGGAAGGTCTGAAGCTGTCGCCTTGGTTCAGGCTCGTTGTGGACAACTTCTTGTTCAAGTGGTGGGACCATGTTGAGAAAAACACCATCAAGGAAGCTGCTGACATGAAAACCATCCACAGGTTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
NUDIX

Protein Analysis

291

Amino Acids

33.18

Weight (kDa)

5.89

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 108 - 243 9.5e-15 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AciI CCGC 5 cut(s) 75, 111, 129, 205, 440
AclI AACGTT 1 cut(s) 382
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 3 cut(s) 359, 555, 773
AcyI GRCGYC 2 cut(s) 180, 192
AfaI GTAC 5 cut(s) 275, 446, 581, 642, 692
AfiI CCNNNNNNNGG 4 cut(s) 182, 596, 673, 861
AgsI TTSAA 1 cut(s) 799
AjnI CCWGG 1 cut(s) 131
AleI CACNNNNGTG 1 cut(s) 412
AluBI AGCT 8 cut(s) 332, 460, 508, 680, 709, 718, 757, 839
AluI AGCT 8 cut(s) 332, 460, 508, 680, 709, 718, 757, 839
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 5 cut(s) 77, 432, 494, 706, 839
ApoI RAATTY 1 cut(s) 316
Asp700I GAANNNNTTC 2 cut(s) 91, 228
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 808
AsuC2I CCSGG 1 cut(s) 739
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 808
BanI GGYRCC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 5 cut(s) 64, 444, 481, 718, 826
BccI CCATC 5 cut(s) 283, 444, 590, 836, 863
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 133
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 739
BfoI RGCGCY 1 cut(s) 205
BisI GCNGC 6 cut(s) 78, 205, 433, 495, 707, 840
BlsI GCNGC 6 cut(s) 79, 206, 434, 496, 708, 841
Bme1390I CCNGG 2 cut(s) 133, 739
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 808
BmiI GGNNCC 2 cut(s) 588, 809
BmrFI CCNGG 2 cut(s) 133, 739
BmsI GCATC 2 cut(s) 667, 724
BpiI GAAGAC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 739
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 694
BsaHI GRCGYC 2 cut(s) 180, 192
BsaJI CCNNGG 4 cut(s) 162, 393, 738, 764
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 596, 673, 861
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 545
BseBI CCWGG 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 4 cut(s) 162, 393, 738, 764
BseGI GGATG 6 cut(s) 71, 244, 436, 520, 658, 855
BseLI CCNNNNNNNGG 4 cut(s) 182, 596, 673, 861
BseNI ACTGG 1 cut(s) 545
BseRI GAGGAG 3 cut(s) 45, 48, 51
BseXI GCAGC 5 cut(s) 64, 444, 481, 718, 826
BshFI GGCC 1 cut(s) 167
BshNI GGYRCC 1 cut(s) 586
BshVI ATCGAT 1 cut(s) 300
BsiSI CCGG 2 cut(s) 183, 738
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 4 cut(s) 182, 596, 673, 861
BsmFI GGGAC 1 cut(s) 821
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 1 cut(s) 167
Bsp119I TTCGAA 1 cut(s) 216
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 4 cut(s) 247, 297, 301, 550
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 5 cut(s) 75, 111, 129, 205, 440
BspANI GGCC 1 cut(s) 167
BspDI ATCGAT 1 cut(s) 300
BspLI GGNNCC 2 cut(s) 588, 809
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 586
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 4 cut(s) 162, 393, 738, 764
BssMI GATC 4 cut(s) 247, 297, 301, 550
BssNI GRCGYC 2 cut(s) 180, 192
BssT1I CCWWGG 3 cut(s) 162, 393, 764
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 2 cut(s) 449, 640
Bst6I CTCTTC 3 cut(s) 71, 97, 638
BstACI GRCGYC 2 cut(s) 180, 192
BstAPI GCANNNNNTGC 1 cut(s) 367
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 184, 575
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 6 cut(s) 71, 244, 436, 520, 658, 855
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 4 cut(s) 250, 300, 304, 553
BstMBI GATC 4 cut(s) 247, 297, 301, 550
BstMWI GCNNNNNNNGC 4 cut(s) 179, 367, 376, 715
BstNI CCWGG 1 cut(s) 133
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 2 cut(s) 131, 737
BstSNI TACGTA 1 cut(s) 694
BstV1I GCAGC 5 cut(s) 64, 444, 481, 718, 826
BstV2I GAAGAC 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 414
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 1 cut(s) 167
BsuTUI ATCGAT 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 6 cut(s) 71, 244, 436, 520, 658, 855
BtsIMutI CAGTG 2 cut(s) 343, 636
Cac8I GCNNGC 2 cut(s) 184, 575
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 808
ClaI ATCGAT 1 cut(s) 300
CseI GACGC 3 cut(s) 179, 188, 200
Csp6I GTAC 5 cut(s) 274, 445, 580, 641, 691
CviAII CATG 8 cut(s) 163, 187, 211, 362, 574, 614, 812, 847
CviQI GTAC 5 cut(s) 274, 445, 580, 641, 691
DpnI GATC 4 cut(s) 249, 299, 303, 552
DpnII GATC 4 cut(s) 247, 297, 301, 550
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 3 cut(s) 71, 97, 638
EarI CTCTTC 3 cut(s) 71, 97, 638
EciI GGCGGA 1 cut(s) 118
Eco105I TACGTA 1 cut(s) 694
Eco130I CCWWGG 3 cut(s) 162, 393, 764
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 808
Eco57I CTGAAG 3 cut(s) 359, 555, 773
EcoRII CCWGG 1 cut(s) 131
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 3 cut(s) 162, 393, 764
ErhI CCWWGG 3 cut(s) 162, 393, 764
FaeI CATG 8 cut(s) 166, 190, 214, 365, 577, 617, 815, 850
FaiI YATR 8 cut(s) 164, 188, 212, 363, 575, 615, 813, 848
FaqI GGGAC 1 cut(s) 821
FatI CATG 8 cut(s) 162, 186, 210, 361, 573, 613, 811, 846
FbaI TGATCA 1 cut(s) 550
Fnu4HI GCNGC 6 cut(s) 78, 205, 433, 495, 707, 840
FokI GGATG 6 cut(s) 58, 251, 423, 527, 645, 842
Fsp4HI GCNGC 6 cut(s) 78, 205, 433, 495, 707, 840
GlaI GCGC 1 cut(s) 203
GluI GCNGC 6 cut(s) 78, 205, 433, 495, 707, 840
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 1 cut(s) 167
HapII CCGG 2 cut(s) 183, 738
HgaI GACGC 3 cut(s) 179, 188, 200
HhaI GCGC 1 cut(s) 204
Hin1I GRCGYC 2 cut(s) 180, 192
Hin1II CATG 8 cut(s) 166, 190, 214, 365, 577, 617, 815, 850
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 4 cut(s) 4, 82, 87, 452
HpaII CCGG 2 cut(s) 183, 738
Hpy166II GTNNAC 4 cut(s) 417, 697, 784, 867
Hpy188I TCNGA 5 cut(s) 313, 457, 595, 663, 753
Hpy188III TCNNGA 2 cut(s) 176, 263
Hpy8I GTNNAC 4 cut(s) 417, 697, 784, 867
Hpy99I CGWCG 2 cut(s) 173, 182
HpyAV CCTTC 7 cut(s) 32, 49, 106, 600, 638, 721, 740
HpyCH4III ACNGT 2 cut(s) 449, 640
HpyCH4IV ACGT 2 cut(s) 382, 693
HpyCH4V TGCA 3 cut(s) 389, 432, 658
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 367, 376, 715
HpySE526I ACGT 2 cut(s) 382, 693
Hsp92I GRCGYC 2 cut(s) 180, 192
Hsp92II CATG 8 cut(s) 166, 190, 214, 365, 577, 617, 815, 850
HspAI GCGC 1 cut(s) 202
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 4 cut(s) 247, 297, 301, 550
LmnI GCTCC 2 cut(s) 178, 703
Lsp1109I GCAGC 5 cut(s) 64, 444, 481, 718, 826
LweI GCATC 2 cut(s) 667, 724
MaeII ACGT 2 cut(s) 382, 693
MaeIII GTNAC 3 cut(s) 260, 281, 397
MalI GATC 4 cut(s) 249, 299, 303, 552
MboI GATC 4 cut(s) 247, 297, 301, 550
MboII GAAGA 7 cut(s) 34, 58, 84, 230, 365, 548, 625
MfeI CAATTG 1 cut(s) 277
MluCI AATT 2 cut(s) 277, 316
MlyI GAGTC 2 cut(s) 13, 76
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 2 cut(s) 91, 228
MseI TTAA 3 cut(s) 351, 651, 871
MslI CAYNNNNRTG 1 cut(s) 412
MspA1I CMGCKG 3 cut(s) 77, 442, 709
MspI CCGG 2 cut(s) 183, 738
MspR9I CCNGG 2 cut(s) 133, 739
MunI CAATTG 1 cut(s) 277
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 133
MwoI GCNNNNNNNGC 4 cut(s) 179, 367, 376, 715
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 1 cut(s) 739
NcoI CCATGG 1 cut(s) 162
NdeII GATC 4 cut(s) 247, 297, 301, 550
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 8 cut(s) 166, 190, 214, 365, 577, 617, 815, 850
NlaIV GGNNCC 2 cut(s) 588, 809
NmuCI GTSAC 2 cut(s) 260, 281
NspI RCATGY 1 cut(s) 577
NspV TTCGAA 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 412
PaeI GCATGC 1 cut(s) 577
PctI GAATGC 1 cut(s) 113
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 91, 228
PfeI GAWTC 2 cut(s) 87, 452
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 6 cut(s) 79, 206, 434, 496, 708, 841
PleI GAGTC 2 cut(s) 12, 76
PpsI GAGTC 2 cut(s) 12, 76
Ppu21I YACGTR 1 cut(s) 694
Psp1406I AACGTT 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 588, 809
PspPI GGNCC 1 cut(s) 808
PsyI GACNNNGTC 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 709
RsaI GTAC 5 cut(s) 275, 446, 581, 642, 692
RsaNI GTAC 5 cut(s) 274, 445, 580, 641, 691
RseI CAYNNNNRTG 1 cut(s) 412
SaqAI TTAA 3 cut(s) 351, 651, 871
SatI GCNGC 6 cut(s) 78, 205, 433, 495, 707, 840
Sau3AI GATC 4 cut(s) 247, 297, 301, 550
Sau96I GGNCC 1 cut(s) 808
SchI GAGTC 2 cut(s) 13, 76
ScrFI CCNGG 2 cut(s) 133, 739
SfaNI GCATC 2 cut(s) 667, 724
SfuI TTCGAA 1 cut(s) 216
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 412
SnaBI TACGTA 1 cut(s) 694
SphI GCATGC 1 cut(s) 577
Sse9I AATT 2 cut(s) 277, 316
SsiI CCGC 5 cut(s) 75, 111, 129, 205, 440
StyD4I CCNGG 2 cut(s) 131, 737
StyI CCWWGG 3 cut(s) 162, 393, 764
TaaI ACNGT 2 cut(s) 449, 640
TaiI ACGT 2 cut(s) 385, 696
TaqI TCGA 3 cut(s) 85, 216, 300
TasI AATT 2 cut(s) 277, 316
TatI WGTACW 2 cut(s) 273, 579
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 2 cut(s) 87, 452
Tru1I TTAA 3 cut(s) 351, 651, 871
Tru9I TTAA 3 cut(s) 351, 651, 871
TscAI CASTG 2 cut(s) 343, 643
TseFI GTSAC 2 cut(s) 260, 281
TseI GCWGC 5 cut(s) 77, 432, 494, 706, 839
Tsp45I GTSAC 2 cut(s) 260, 281
TspDTI ATGAA 3 cut(s) 531, 630, 863
TspRI CASTG 2 cut(s) 343, 643
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 577
XmnI GAANNNNTTC 2 cut(s) 91, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.