pycom05g00770

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018116
Physical Location & Seq
Reverse (-)
968883 .. 969845
963 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00770.1

Sequence Viewer

Length: 495 bp
ATGTCTAATACTGCTGGTCAGGTCATACGCTGCAGAGCTGCTGTAGCTTGGGAAGCAGGGAAGCCACTGGTGATTGAAGAAGTCGAGGTGGCACCACCACAAGCAAATGAAGTCCGTTTGAAGATCCTTTTCACCTCCTTGTGCCACACTGATGTCTACTTTTGGGAAGCCAAGGGACAAAACCCTTTATTTCCTAGAATTTATGGTCATGAGGCAGGAGGGATTGTGGAGAGTGTTGGTGAGGGAGTGACGGATCTGAAAGCCGGTGATCATGTCCTGCCGGTGTTCACAGGAGAATGCAAGGACTGCGCTCACTGCAAATCAGAAGAGAGCAACATGTGTGACCTCCTCAGGATAAACACTGACAGGGGAGTGATGCTCAGTGATGGCAAATCAAGATTTTCAATCAAAGGCAAGCCTATCTACCACTTTGTTGGGACTTCCACCTTCAGTGAGTACACTGTTGTTCATGTTGGCTGCCTTGCCAATAATTGA

Protein Analysis

165

Amino Acids

17.93

Weight (kDa)

5.85

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 35 - 158 1.2e-19 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccI GTMKAC 1 cut(s) 156
AclWI GGATC 2 cut(s) 118, 261
AcsI RAATTY 1 cut(s) 198
AcuI CTGAAG 1 cut(s) 433
AfaI GTAC 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 3 cut(s) 77, 121, 405
AluBI AGCT 2 cut(s) 38, 47
AluI AGCT 2 cut(s) 38, 47
AlwI GGATC 2 cut(s) 118, 261
ApeKI GCWGC 3 cut(s) 30, 38, 477
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 311
AsuHPI GGTGA 4 cut(s) 82, 124, 251, 278
AxyI CCTNAGG 1 cut(s) 350
BanI GGYRCC 1 cut(s) 91
BbvI GCAGC 3 cut(s) 17, 25, 464
BccI CCATC 1 cut(s) 380
BclI TGATCA 1 cut(s) 268
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 2 cut(s) 31, 42
BisI GCNGC 3 cut(s) 31, 39, 478
BlsI GCNGC 3 cut(s) 32, 40, 479
BmiI GGNNCC 1 cut(s) 93
BmsI GCATC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 363, 395
BsaJI CCNNGG 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse118I RCCGGY 2 cut(s) 263, 280
Bse1I ACTGG 1 cut(s) 72
Bse21I CCTNAGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 171
BseMII CTCAG 2 cut(s) 364, 394
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 3 cut(s) 17, 25, 464
BshNI GGYRCC 1 cut(s) 91
BsiSI CCGG 2 cut(s) 264, 281
BslFI GGGAC 2 cut(s) 189, 451
BsmFI GGGAC 2 cut(s) 189, 451
BsmI GAATGC 1 cut(s) 302
Bsp143I GATC 3 cut(s) 123, 253, 268
BspCNI CTCAG 2 cut(s) 363, 393
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 93
BspMAI CTGCAG 1 cut(s) 35
BspPI GGATC 2 cut(s) 118, 261
BspT107I GGYRCC 1 cut(s) 91
BsrFI RCCGGY 2 cut(s) 263, 280
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 2 cut(s) 263, 280
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 3 cut(s) 123, 253, 268
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 2 cut(s) 350, 380
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 3 cut(s) 126, 256, 271
BstMBI GATC 3 cut(s) 123, 253, 268
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 306, 315
BstNSI RCATGY 1 cut(s) 340
BstSFI CTRYAG 2 cut(s) 31, 42
BstV1I GCAGC 3 cut(s) 17, 25, 464
BstX2I RGATCY 2 cut(s) 123, 253
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 2 cut(s) 123, 253
Bsu36I CCTNAGG 1 cut(s) 350
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 7 cut(s) 65, 147, 313, 360, 388, 457, 459
Cac8I GCNNGC 1 cut(s) 416
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 2 cut(s) 263, 280
Csp6I GTAC 1 cut(s) 457
CviAII CATG 4 cut(s) 209, 272, 337, 470
CviJI RGCY 7 cut(s) 38, 47, 64, 170, 263, 418, 477
CviKI_1 RGCY 7 cut(s) 38, 47, 64, 170, 263, 418, 477
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 2 cut(s) 350, 380
DpnI GATC 3 cut(s) 125, 255, 270
DpnII GATC 3 cut(s) 123, 253, 268
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 433
Eco81I CCTNAGG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 4 cut(s) 212, 275, 340, 473
FaiI YATR 6 cut(s) 26, 204, 210, 273, 338, 471
FaqI GGGAC 2 cut(s) 189, 451
FatI CATG 4 cut(s) 208, 271, 336, 469
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 31, 39, 478
Fsp4HI GCNGC 3 cut(s) 31, 39, 478
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 310
GluI GCNGC 3 cut(s) 31, 39, 478
HapII CCGG 2 cut(s) 264, 281
HhaI GCGC 1 cut(s) 311
Hin1II CATG 4 cut(s) 212, 275, 340, 473
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HpaII CCGG 2 cut(s) 264, 281
HphI GGTGA 4 cut(s) 82, 124, 251, 278
Hpy166II GTNNAC 3 cut(s) 157, 288, 459
Hpy188I TCNGA 2 cut(s) 258, 325
Hpy188III TCNNGA 3 cut(s) 209, 352, 396
Hpy8I GTNNAC 3 cut(s) 157, 288, 459
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 3 cut(s) 33, 300, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 306, 315
HpyF3I CTNAG 2 cut(s) 350, 380
Hsp92II CATG 4 cut(s) 212, 275, 340, 473
HspAI GCGC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 123, 253, 268
Lsp1109I GCAGC 3 cut(s) 17, 25, 464
LweI GCATC 1 cut(s) 366
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 247, 341
MalI GATC 3 cut(s) 125, 255, 270
MboI GATC 3 cut(s) 123, 253, 268
MboII GAAGA 3 cut(s) 89, 133, 338
MflI RGATCY 2 cut(s) 123, 253
MluCI AATT 2 cut(s) 198, 490
MnlI CCTC 7 cut(s) 79, 145, 205, 212, 235, 356, 359
MslI CAYNNNNRTG 1 cut(s) 150
MspI CCGG 2 cut(s) 264, 281
Mva1269I GAATGC 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 306, 315
NdeII GATC 3 cut(s) 123, 253, 268
NlaIII CATG 4 cut(s) 212, 275, 340, 473
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 247, 341
NspI RCATGY 1 cut(s) 340
PagI TCATGA 1 cut(s) 208
PciI ACATGT 1 cut(s) 336
PctI GAATGC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 32, 40, 479
PscI ACATGT 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 93
PstI CTGCAG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 123, 253
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 150
SatI GCNGC 3 cut(s) 31, 39, 478
Sau3AI GATC 3 cut(s) 123, 253, 268
SetI ASST 7 cut(s) 24, 40, 49, 90, 137, 348, 449
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 2 cut(s) 31, 42
SmiMI CAYNNNNRTG 1 cut(s) 150
Sse9I AATT 2 cut(s) 198, 490
SspMI CTAG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 1 cut(s) 84
TasI AATT 2 cut(s) 198, 490
TatI WGTACW 1 cut(s) 456
TscAI CASTG 7 cut(s) 72, 154, 320, 367, 388, 457, 466
TseFI GTSAC 2 cut(s) 247, 341
TseI GCWGC 3 cut(s) 30, 38, 477
Tsp45I GTSAC 2 cut(s) 247, 341
TspDTI ATGAA 2 cut(s) 123, 458
TspGWI ACGGA 2 cut(s) 104, 266
TspRI CASTG 7 cut(s) 72, 154, 320, 367, 388, 457, 466
XapI RAATTY 1 cut(s) 198
XceI RCATGY 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.