pycom05g00770

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018116
Physical Location & Seq
Reverse (-)
968883 .. 969845
963 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGTCTAATACTGCTGGTCAGGTCATACGCTGCAGAGCTGCTGTAGCTTGGGAAGCAGGGAAGCCACTGGTGATTGAAGAAGTCGAGGTGGCACCACCACAAGCAAATGAAGTCCGTTTGAAGATCCTTTTCACCTCCTTGTGCCACACTGATGTCTACTTTTGGGAAGCCAAGGGACAAAACCCTTTATTTCCTAGAATTTATGGTCATGAGGCAGGAGGGATTGTGGAGAGTGTTGGTGAGGGAGTGACGGATCTGAAAGCCGGTGATCATGTCCTGCCGGTGTTCACAGGAGAATGCAAGGACTGCGCTCACTGCAAATCAGAAGAGAGCAACATGTGTGACCTCCTCAGGATAAACACTGACAGGGGAGTGATGCTCAGTGATGGCAAATCAAGATTTTCAATCAAAGGCAAGCCTATCTACCACTTTGTTGGGACTTCCACCTTCAGTGAGTACACTGTTGTTCATGTTGGCTGCCTTGCCAATAATTGA

Protein Analysis

165

Amino Acids

17.93

Weight (kDa)

5.85

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 35 - 158 1.2e-19 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccI GTMKAC 1 cut(s) 156
AclWI GGATC 2 cut(s) 118, 261
AcsI RAATTY 1 cut(s) 198
AcuI CTGAAG 1 cut(s) 433
AfaI GTAC 1 cut(s) 458
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 3 cut(s) 77, 121, 405
AluBI AGCT 2 cut(s) 38, 47
AluI AGCT 2 cut(s) 38, 47
AlwI GGATC 2 cut(s) 118, 261
ApeKI GCWGC 3 cut(s) 30, 38, 477
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 311
AsuHPI GGTGA 4 cut(s) 82, 124, 251, 278
AxyI CCTNAGG 1 cut(s) 350
BanI GGYRCC 1 cut(s) 91
BbvI GCAGC 3 cut(s) 17, 25, 464
BccI CCATC 1 cut(s) 380
BclI TGATCA 1 cut(s) 268
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 2 cut(s) 31, 42
BisI GCNGC 3 cut(s) 31, 39, 478
BlsI GCNGC 3 cut(s) 32, 40, 479
BmiI GGNNCC 1 cut(s) 93
BmsI GCATC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 363, 395
BsaJI CCNNGG 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse118I RCCGGY 2 cut(s) 263, 280
Bse1I ACTGG 1 cut(s) 72
Bse21I CCTNAGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 171
BseMII CTCAG 2 cut(s) 364, 394
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 3 cut(s) 17, 25, 464
BshNI GGYRCC 1 cut(s) 91
BsiSI CCGG 2 cut(s) 264, 281
BslFI GGGAC 2 cut(s) 189, 451
BsmFI GGGAC 2 cut(s) 189, 451
BsmI GAATGC 1 cut(s) 302
Bsp143I GATC 3 cut(s) 123, 253, 268
BspCNI CTCAG 2 cut(s) 363, 393
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 93
BspMAI CTGCAG 1 cut(s) 35
BspPI GGATC 2 cut(s) 118, 261
BspT107I GGYRCC 1 cut(s) 91
BsrFI RCCGGY 2 cut(s) 263, 280
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 2 cut(s) 263, 280
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 3 cut(s) 123, 253, 268
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 2 cut(s) 350, 380
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 3 cut(s) 126, 256, 271
BstMBI GATC 3 cut(s) 123, 253, 268
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 306, 315
BstNSI RCATGY 1 cut(s) 340
BstSFI CTRYAG 2 cut(s) 31, 42
BstV1I GCAGC 3 cut(s) 17, 25, 464
BstX2I RGATCY 2 cut(s) 123, 253
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 2 cut(s) 123, 253
Bsu36I CCTNAGG 1 cut(s) 350
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 7 cut(s) 65, 147, 313, 360, 388, 457, 459
Cac8I GCNNGC 1 cut(s) 416
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 2 cut(s) 263, 280
Csp6I GTAC 1 cut(s) 457
CviAII CATG 4 cut(s) 209, 272, 337, 470
CviJI RGCY 7 cut(s) 38, 47, 64, 170, 263, 418, 477
CviKI_1 RGCY 7 cut(s) 38, 47, 64, 170, 263, 418, 477
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 2 cut(s) 350, 380
DpnI GATC 3 cut(s) 125, 255, 270
DpnII GATC 3 cut(s) 123, 253, 268
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 433
Eco81I CCTNAGG 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 4 cut(s) 212, 275, 340, 473
FaiI YATR 6 cut(s) 26, 204, 210, 273, 338, 471
FaqI GGGAC 2 cut(s) 189, 451
FatI CATG 4 cut(s) 208, 271, 336, 469
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 31, 39, 478
Fsp4HI GCNGC 3 cut(s) 31, 39, 478
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 310
GluI GCNGC 3 cut(s) 31, 39, 478
HapII CCGG 2 cut(s) 264, 281
HhaI GCGC 1 cut(s) 311
Hin1II CATG 4 cut(s) 212, 275, 340, 473
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HpaII CCGG 2 cut(s) 264, 281
HphI GGTGA 4 cut(s) 82, 124, 251, 278
Hpy166II GTNNAC 3 cut(s) 157, 288, 459
Hpy188I TCNGA 2 cut(s) 258, 325
Hpy188III TCNNGA 3 cut(s) 209, 352, 396
Hpy8I GTNNAC 3 cut(s) 157, 288, 459
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 3 cut(s) 33, 300, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 306, 315
HpyF3I CTNAG 2 cut(s) 350, 380
Hsp92II CATG 4 cut(s) 212, 275, 340, 473
HspAI GCGC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 123, 253, 268
Lsp1109I GCAGC 3 cut(s) 17, 25, 464
LweI GCATC 1 cut(s) 366
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 247, 341
MalI GATC 3 cut(s) 125, 255, 270
MboI GATC 3 cut(s) 123, 253, 268
MboII GAAGA 3 cut(s) 89, 133, 338
MflI RGATCY 2 cut(s) 123, 253
MluCI AATT 2 cut(s) 198, 490
MnlI CCTC 7 cut(s) 79, 145, 205, 212, 235, 356, 359
MslI CAYNNNNRTG 1 cut(s) 150
MspI CCGG 2 cut(s) 264, 281
Mva1269I GAATGC 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 306, 315
NdeII GATC 3 cut(s) 123, 253, 268
NlaIII CATG 4 cut(s) 212, 275, 340, 473
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 247, 341
NspI RCATGY 1 cut(s) 340
PagI TCATGA 1 cut(s) 208
PciI ACATGT 1 cut(s) 336
PctI GAATGC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 32, 40, 479
PscI ACATGT 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 93
PstI CTGCAG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 123, 253
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 150
SatI GCNGC 3 cut(s) 31, 39, 478
Sau3AI GATC 3 cut(s) 123, 253, 268
SetI ASST 7 cut(s) 24, 40, 49, 90, 137, 348, 449
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 2 cut(s) 31, 42
SmiMI CAYNNNNRTG 1 cut(s) 150
Sse9I AATT 2 cut(s) 198, 490
SspMI CTAG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 1 cut(s) 84
TasI AATT 2 cut(s) 198, 490
TatI WGTACW 1 cut(s) 456
TscAI CASTG 7 cut(s) 72, 154, 320, 367, 388, 457, 466
TseFI GTSAC 2 cut(s) 247, 341
TseI GCWGC 3 cut(s) 30, 38, 477
Tsp45I GTSAC 2 cut(s) 247, 341
TspDTI ATGAA 2 cut(s) 123, 458
TspGWI ACGGA 2 cut(s) 104, 266
TspRI CASTG 7 cut(s) 72, 154, 320, 367, 388, 457, 466
XapI RAATTY 1 cut(s) 198
XceI RCATGY 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.