pycom05g01830

Ankyrin repeat domain-containing protein EMB506

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011420
Physical Location & Seq
Forward (+)
2185150 .. 2187297
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 996 bp
ATGGGCACGGTTTCAATTTCCGCATTGTCGGGTCTAAATTCAATTTCCATCCCCAGAGATTACAACCCTTTTGCAATTTTTAATAAAGCCGGCGTCTTGACAGCGTGTAGTACAAGGAGTGGAAGCTTTAGGTTCCATGGCGCTCAGAGGCACTCAAAGCTGAGGACTTTTTCGGTCAGGTCGTTGAGCCCGACTGCTTCGTTTCGGACCCAGGTGGGAACTTGGGAAGCACCCGACGATGGCAGCGGCAGCGAAGACGAGGAGGATGAAGAAGAAGAAGAAGAAGAATTGGGTTTCCAAAGTGAATGGGAGGAAGAAGAGGGAAAAGGTGTTCCAGCTACTGCTACTGATGCTGCTGCTGCTGCTGCTGTTGATAAGTCCACAACAAACAAGTATGAAGAGGATCTTGTAAAAGAAGTTGAACAGCTCTTGGGGCAAGAAGAAATAGCAATTTTGCAAAAGCATGCGGCTCCCAACATCAAGAAAATATCAACGGAAAAATGGAACCCGATACATACTCTTGCACTGTCAGGGATGGTAACCTTCATGGACAAGCTACTTGCAGATGGTTTAGATATTGACGTTGTTGATAAGGATGGTACAACTGCTCTTCATACTGCAATCATAGCCAAAAAGGAAGCTGTAATTAGTCATCTGCTAAGAAAAGGTGCAAGTCCTCATGTCAGAGACAGGGATGGTGCTACGCCGCTTCATTACGCAGTACATGTAGGCACCAAGCGGATTGTCAAGTTATTGATGAAATATAAAGTTGATGTCAATATTGCAGATAATGATGGATGGACTCCGTTGCATGTTGCTGTTCAAAGTAGAAACAGAGACATTGTGAAAATTTTGTTAGTCAATGGTGCAGATAAGAGCAGAAGAAATAAGGACGGCAGAACTCCGTTAGATCTGGGTTTATGTTATGGGAAAGATTTCAAGTCCTATGACCTCACCAAGCTACTGAAGACGGTGCCAATGGACAGAGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

332

Amino Acids

36.43

Weight (kDa)

5.74

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 154 - 230 4.3e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 214 - 295 2.3e-20 Ankyrin repeats (3 copies)
Ank_5 PF13857 219 - 273 4.6e-10 Ankyrin repeats (many copies)
Ank PF00023 232 - 264 9.7e-07 Ankyrin repeat
Ank_4 PF13637 247 - 286 2.8e-07 Ankyrin repeats (many copies)
Ank PF00023 265 - 297 6.8e-08 Ankyrin repeat
Ank_5 PF13857 268 - 305 1.3e-06 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40160 AT5G40160
fragaria_vesca FvH4_5g31250 FvH4_5g31250 FvH4_5g31250
malus_domestica MD05G1026500.v1.1
prunus_persica Prupe.3G132600_v2.0.a1
pyrus_communis pycom05g01830
rosa_chinensis RchiOBHm_Chr7g0226491
rosa_laevigata RLG00000001726
rosa_multiflora Rmu_sc0001423.1_g000067 Rmu_sc0001470.1_g000013
rosa_rugosa Rorug07G0237500
rosa_samantha Rh4CG052600 Rh7AG384700 Rh7BG371500 Rh7CG404000 Rh7DG383700
rosa_wichuraiana Rw4G003890 Rw7G032310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 731, 973
AciI CCGC 5 cut(s) 21, 246, 467, 707, 739
AclWI GGATC 1 cut(s) 411
AcsI RAATTY 2 cut(s) 37, 849
AcuI CTGAAG 1 cut(s) 986
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 3 cut(s) 112, 601, 723
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 5 cut(s) 15, 42, 422, 824, 940
AjnI CCWGG 1 cut(s) 210
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 7 cut(s) 126, 160, 338, 427, 556, 641, 961
AluI AGCT 7 cut(s) 126, 160, 338, 427, 556, 641, 961
Alw26I GTCTC 2 cut(s) 681, 831
AlwI GGATC 1 cut(s) 411
AlwNI CAGNNNCTG 1 cut(s) 341
ApeKI GCWGC 7 cut(s) 243, 249, 353, 356, 359, 362, 365
ApoI RAATTY 2 cut(s) 37, 849
Asp700I GAANNNNTTC 1 cut(s) 935
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 946
AvaII GGWCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 2 cut(s) 731, 973
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 261, 974
BbvCI CCTCAGC 1 cut(s) 161
BbvI GCAGC 7 cut(s) 255, 261, 340, 343, 346, 349, 352
BccI CCATC 8 cut(s) 56, 233, 529, 560, 590, 689, 788, 792
BceAI ACGGC 1 cut(s) 910
BciT130I CCWGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 681, 831
BfoI RGCGCY 1 cut(s) 144
BglII AGATCT 1 cut(s) 910
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 6 cut(s) 134, 209, 471, 506, 733, 975
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 1 cut(s) 340
BpiI GAAGAC 2 cut(s) 261, 974
Bpu10I CCTNAGC 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 136, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 89
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 136, 210
BseGI GGATG 6 cut(s) 48, 271, 540, 601, 700, 803
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 2 cut(s) 152, 158
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 7 cut(s) 255, 261, 340, 343, 346, 349, 352
BsgI GTGCAG 1 cut(s) 888
BshNI GGYRCC 2 cut(s) 731, 973
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 681, 831
Bsp1286I GDGCHC 2 cut(s) 8, 191
Bsp143I GATC 2 cut(s) 403, 910
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 5 cut(s) 21, 246, 467, 707, 739
BspCNI CTCAG 2 cut(s) 153, 157
BspLI GGNNCC 6 cut(s) 134, 209, 471, 506, 733, 975
BspPI GGATC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 2 cut(s) 731, 973
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 136, 210
BssMI GATC 2 cut(s) 403, 910
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 3 cut(s) 10, 528, 973
Bst6I CTCTTC 3 cut(s) 312, 393, 615
BstACI GRCGYC 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 91, 465
BstDEI CTNAG 3 cut(s) 144, 161, 659
BstDSI CCRYGG 1 cut(s) 136
BstEII GGTNACC 1 cut(s) 538
BstF5I GGATG 6 cut(s) 48, 271, 540, 601, 700, 803
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 2 cut(s) 406, 913
BstMAI GTCTC 2 cut(s) 681, 831
BstMBI GATC 2 cut(s) 403, 910
BstMWI GCNNNNNNNGC 8 cut(s) 157, 249, 350, 359, 362, 365, 433, 626
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 3 cut(s) 467, 728, 815
BstPI GGTNACC 1 cut(s) 538
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 7 cut(s) 255, 261, 340, 343, 346, 349, 352
BstV2I GAAGAC 2 cut(s) 261, 974
BstX2I RGATCY 2 cut(s) 403, 910
BstYI RGATCY 2 cut(s) 403, 910
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 6 cut(s) 48, 271, 540, 601, 700, 803
BtsIMutI CAGTG 1 cut(s) 524
Cac8I GCNNGC 2 cut(s) 91, 465
CaiI CAGNNNCTG 1 cut(s) 341
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 1 cut(s) 82
Csp6I GTAC 3 cut(s) 111, 600, 722
CviAII CATG 6 cut(s) 137, 464, 547, 680, 725, 812
CviQI GTAC 3 cut(s) 111, 600, 722
DdeI CTNAG 3 cut(s) 144, 161, 659
DpnI GATC 2 cut(s) 405, 912
DpnII GATC 2 cut(s) 403, 910
Eam1104I CTCTTC 3 cut(s) 312, 393, 615
EarI CTCTTC 3 cut(s) 312, 393, 615
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 986
Eco91I GGTNACC 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 6 cut(s) 140, 467, 550, 683, 728, 815
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 6 cut(s) 136, 463, 546, 679, 724, 811
FokI GGATG 6 cut(s) 35, 278, 547, 608, 707, 810
FriOI GRGCYC 1 cut(s) 191
GlaI GCGC 1 cut(s) 142
HaeII RGCGCY 1 cut(s) 144
HapII CCGG 1 cut(s) 90
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 6 cut(s) 140, 467, 550, 683, 728, 815
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 1 cut(s) 802
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 946
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 3 cut(s) 147, 207, 686
Hpy188III TCNNGA 2 cut(s) 97, 481
Hpy8I GTNNAC 1 cut(s) 381
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 553
HpyCH4III ACNGT 3 cut(s) 10, 528, 973
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 9 cut(s) 74, 457, 524, 563, 620, 671, 785, 811, 869
HpyF10VI GCNNNNNNNGC 8 cut(s) 157, 249, 350, 359, 362, 365, 433, 626
HpyF3I CTNAG 3 cut(s) 144, 161, 659
HpySE526I ACGT 1 cut(s) 582
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 6 cut(s) 140, 467, 550, 683, 728, 815
HspAI GCGC 1 cut(s) 141
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Kzo9I GATC 2 cut(s) 403, 910
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 1 cut(s) 475
LpnPI CCDG 9 cut(s) 67, 103, 163, 197, 224, 348, 516, 676, 899
Lsp1109I GCAGC 7 cut(s) 255, 261, 340, 343, 346, 349, 352
LweI GCATC 1 cut(s) 340
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 1 cut(s) 538
MalI GATC 2 cut(s) 405, 912
MboI GATC 2 cut(s) 403, 910
MflI RGATCY 2 cut(s) 403, 910
MhlI GDGCHC 2 cut(s) 8, 191
MluCI AATT 8 cut(s) 15, 37, 42, 75, 287, 450, 645, 849
MlyI GAGTC 1 cut(s) 796
MnlI CCTC 9 cut(s) 141, 156, 253, 256, 304, 313, 394, 687, 962
MroNI GCCGGC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 935
MseI TTAA 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 246
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 8 cut(s) 157, 249, 350, 359, 362, 365, 433, 626
NaeI GCCGGC 1 cut(s) 91
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 403, 910
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 6 cut(s) 140, 467, 550, 683, 728, 815
NlaIV GGNNCC 6 cut(s) 134, 209, 471, 506, 733, 975
NspI RCATGY 3 cut(s) 467, 728, 815
PaeI GCATGC 1 cut(s) 467
PciI ACATGT 1 cut(s) 724
PciSI GCTCTTC 1 cut(s) 615
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 188
PdiI GCCGGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 935
PleI GAGTC 1 cut(s) 796
PpsI GAGTC 1 cut(s) 796
PscI ACATGT 1 cut(s) 724
Psp6I CCWGG 1 cut(s) 210
PspEI GGTNACC 1 cut(s) 538
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 6 cut(s) 134, 209, 471, 506, 733, 975
PspPI GGNCC 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 2 cut(s) 403, 910
RsaI GTAC 3 cut(s) 112, 601, 723
RsaNI GTAC 3 cut(s) 111, 600, 722
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 81
Sau3AI GATC 2 cut(s) 403, 910
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 1 cut(s) 796
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 8, 191
SfaNI GCATC 1 cut(s) 340
SinI GGWCC 1 cut(s) 207
SphI GCATGC 1 cut(s) 467
Sse9I AATT 8 cut(s) 15, 37, 42, 75, 287, 450, 645, 849
SsiI CCGC 5 cut(s) 21, 246, 467, 707, 739
SspI AATATT 1 cut(s) 781
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 10, 528, 973
TaiI ACGT 1 cut(s) 585
TaqII GACCGA 1 cut(s) 163
TasI AATT 8 cut(s) 15, 37, 42, 75, 287, 450, 645, 849
TatI WGTACW 2 cut(s) 110, 721
TauI GCSGC 3 cut(s) 249, 470, 709
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 1 cut(s) 531
TseI GCWGC 7 cut(s) 243, 249, 353, 356, 359, 362, 365
TspDTI ATGAA 6 cut(s) 282, 411, 535, 602, 701, 773
TspGWI ACGGA 3 cut(s) 509, 795, 894
TspRI CASTG 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 2 cut(s) 37, 849
XceI RCATGY 3 cut(s) 467, 728, 815
XmnI GAANNNNTTC 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.