Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0012776

Physical Location & Seq

Genomic Coordinates Forward (+)

4096951 .. 4100695

Gene Structure 3745 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03530.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGCTATTGCAGCTTACTCTGTTCAGACTGCTCTCTCGTTCAAATCCCTCATCATCCCCGAACCCATCAAACCTCATAACCACCACCACTCGGAGCCCCACCTCCGAACCCAGAAACCCATCTCGGCCGTCCATGCCGCCGACCCTTCAAAGTCCTCCGAATCACCCTTCAAACCACAACCCAAATGGACGCTGGATAGCTGGACGGCGAAGAAGGCGATGCAGCTTCCTGAATACCCAGATCAGCAGGAGCTGCAGTCGGTTCTCAACACCCTCGAGAGCTTCCCGCCGATTGTGTTCGCCGGAGAGGCGAGGAGCTTGGAAGAGAAACTGGGTCAGGCTGCTAAGGGGCAGGCCTTCCTTCTTCAGGGCGGTGATTGCGCAGAGAGCTTCAAGGAATTCAATGCTAACAATATAAGGGACACCTTCCGTGTGCTTCTTCAGATGGGTGTAGTGCTTATGTTTGGTGGCCAAATGCCTGTTATTAAGGTAGGGAGAATGGCTGGCCAGTTCGCAAAGCCAAGATCGGACCAATTTGAAGAGAAAAATGGTGTCAAGCTGCCTAGTTACAGAGGTGACAATGTCAATGGTGATGCATTTGATGAAAAAGAAAGAATTCCGGACCCCAGCAGGATGATTAGGGCTTATTGCCAATCTGTGGCAACTTTGAACCTCCTGCGGGCATTTTCAACTGGAGGCTATGCAGCCATGCAGAGAGTCATCCACTGGAATTTGGATTTTACAGAGCATAGCGAGCAAGGGGATAGATACCGCGAGTTGGCTAACCGGGTGGATGAGGTCCTTGGATTTATGGCTGCTGCTGGACTCACAGTTGACCACCCAATCATGACCTCAACAGAGTTTTGGACATCTCATGAATGCTTGCTTCTGCCTTATGAACAAGCACTTACTAGGGAGGATTCTACTTCTGGGCTTTACTATGACTGTTCTGCTCACATGCTTTGGGTTGGGGAACGCACCCGTCAACTCGATGGTGCACATGTGGAGTTTCTGAGAGGAGTTGCTAATCCCCTTGGCATCAAGGTGAGTGATAAAATGGATCCACATGAACTTGTTAGGCTCATAGAGATCCTAAACCCCAAGAACAAACCAGGAAGAATAACCGTAATTGTGAGAATGGGAGCTGAGAACATGAGAGTGAAGCTTCCTCATCTGATCAGGGCAGTTCGTGGAGCTGGTCAAATTGTCACTTGGGTTAGTGACCCTATGCATGGGAACACTATCAAAGCTCCGTGTGGACTCAAAACTCGTTCCTTCGATGCAATACGGGCTGAGTTGAGAGCGTTCTTTGATGTCCATGATCAAGAAGGAAGCTACCCAGGAGGTGCTCATTTAGAGATGACAGGGCAAAACGTGACGGAATGTGTAGGAGGTTCACGAACTATAACGTATAATGATCTGAGTTCACGGTACCACACCCATTGTGACCCAAGGCTCAACGCTTCCCAATCTCTTGAACTGGCCTTTATCATTGCAGAGCGACTACGGAAGAGAAGGCTCGGATCGAGGCACTTTTCAGCGTCTGCTGTTATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0031976 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00400 ko01100 ko01110 ko01130 ko01230 ko02024 map00400 map01100 map01110 map01130 map01230 map02024

Pfam Domains

Protein Families

DAHP_synth_2

Protein Analysis

519

Amino Acids

57.9

Weight (kDa)

7.72

Isoelectric Point (pI)

37.26

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33510
fragaria_vesca	FvH4_5g20460
malus_domestica	MD04G1055100.v1.1 MD05G1042700.v1.1
prunus_persica	Prupe.5G055000_v2.0.a1 Prupe.8G064200_v2.0.a1
pyrus_communis	pycom05g03530
rosa_chinensis	RchiOBHm_Chr7g0205781
rosa_laevigata	RLG00000003355
rosa_multiflora	Rmu_co8369421.1_g000001 Rmu_sc0000076.1_g000051 Rmu_sc0008518.1_g000012
rosa_roxburghii	Rroxscaffold_3G00251990
rosa_rugosa	Rorug07G0093000
rosa_samantha	Rh7AG224500 Rh7CG238100 Rh7DG231200
rosa_wichuraiana	Rw7G019360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1431
AccB1I	GGYRCC	1 cut(s)	1431
AccB7I	CCANNNNNTGG	1 cut(s)	658
AccII	CGCG	1 cut(s)	774
AccIII	TCCGGA	1 cut(s)	619
AciI	CCGC	5 cut(s)	138, 287, 372, 679, 772
AclWI	GGATC	4 cut(s)	1055, 1068, 1084, 1531
AcoI	YGGCCR	3 cut(s)	126, 469, 505
AcsI	RAATTY	3 cut(s)	398, 615, 730
AcuI	CTGAAG	2 cut(s)	350, 425
AfaI	GTAC	1 cut(s)	1433
AfiI	CCNNNNNNNGG	6 cut(s)	91, 367, 658, 679, 778, 1232
AflIII	ACRYGT	1 cut(s)	1000
AgsI	TTSAA	9 cut(s)	43, 150, 172, 394, 403, 539, 670, 690, 1478
AjnI	CCWGG	2 cut(s)	1111, 1339
Alw21I	GWGCWC	2 cut(s)	1000, 1351
Alw44I	GTGCAC	1 cut(s)	996
AlwI	GGATC	4 cut(s)	1055, 1068, 1084, 1531
AlwNI	CAGNNNCTG	2 cut(s)	253, 1544
Ama87I	CYCGRG	1 cut(s)	275
Aor13HI	TCCGGA	1 cut(s)	619
AoxI	GGCC	5 cut(s)	126, 354, 469, 505, 1482
ApaLI	GTGCAC	1 cut(s)	996
ApeKI	GCWGC	8 cut(s)	11, 223, 253, 341, 559, 704, 815, 818
ApoI	RAATTY	3 cut(s)	398, 615, 730
Asp700I	GAANNNNTTC	1 cut(s)	615
Asp718I	GGTACC	1 cut(s)	1431
AspLEI	GCGC	1 cut(s)	383
AspS9I	GGNCC	3 cut(s)	529, 622, 799
AsuC2I	CCSGG	1 cut(s)	788
AsuHPI	GGTGA	5 cut(s)	156, 386, 587, 602, 1057
AvaI	CYCGRG	1 cut(s)	275
AvaII	GGWCC	3 cut(s)	529, 622, 799
BaeGI	GKGCMC	1 cut(s)	1000
BalI	TGGCCA	2 cut(s)	471, 507
BamHI	GGATCC	1 cut(s)	1060
BanI	GGYRCC	1 cut(s)	1431
BanII	GRGCYC	1 cut(s)	99
Bbv12I	GWGCWC	2 cut(s)	1000, 1351
BbvI	GCAGC	8 cut(s)	23, 235, 240, 328, 546, 716, 802, 805
BccI	CCATC	4 cut(s)	74, 128, 439, 986
BceAI	ACGGC	2 cut(s)	113, 222
BciT130I	CCWGG	2 cut(s)	1113, 1341
BclI	TGATCA	2 cut(s)	1176, 1321
BcnI	CCSGG	1 cut(s)	788
BfaI	CTAG	2 cut(s)	564, 912
BfmI	CTRYAG	1 cut(s)	254
BglI	GCCNNNNNGGC	1 cut(s)	308
BisI	GCNGC	9 cut(s)	12, 138, 224, 254, 342, 560, 705, 816, 819
BlsI	GCNGC	9 cut(s)	13, 139, 225, 255, 343, 561, 706, 817, 820
Bme1390I	CCNGG	3 cut(s)	788, 1113, 1341
Bme18I	GGWCC	3 cut(s)	529, 622, 799
BmeT110I	CYCGRG	1 cut(s)	275
BmgT120I	GGNCC	3 cut(s)	529, 622, 799
BmiI	GGNNCC	4 cut(s)	96, 624, 1062, 1433
BmrFI	CCNGG	3 cut(s)	788, 1113, 1341
BmrI	ACTGGG	1 cut(s)	341
BmsI	GCATC	4 cut(s)	210, 583, 1047, 1270
BmuI	ACTGGG	1 cut(s)	341
BpmI	CTGGAG	1 cut(s)	714
Bpu10I	CCTNAGC	1 cut(s)	345
BpuMI	CCSGG	1 cut(s)	788
BsaJI	CCNNGG	4 cut(s)	802, 1033, 1339, 1451
BsaWI	WCCGGW	1 cut(s)	619
BsaXI	ACNNNNNCTCC	2 cut(s)	242, 272
Bsc4I	CCNNNNNNNGG	6 cut(s)	91, 367, 658, 679, 778, 1232
Bse1I	ACTGG	5 cut(s)	336, 508, 697, 731, 1485
Bse3DI	GCAATG	1 cut(s)	1491
BseAI	TCCGGA	1 cut(s)	619
BseBI	CCWGG	2 cut(s)	1113, 1341
BseDI	CCNNGG	4 cut(s)	802, 1033, 1339, 1451
BseGI	GGATG	4 cut(s)	54, 639, 720, 799
BseLI	CCNNNNNNNGG	6 cut(s)	91, 367, 658, 679, 778, 1232
BseMI	GCAATG	1 cut(s)	1491
BseMII	CTCAG	4 cut(s)	1004, 1137, 1284, 1412
BseNI	ACTGG	5 cut(s)	336, 508, 697, 731, 1485
BseRI	GAGGAG	2 cut(s)	328, 1032
BseSI	GKGCMC	1 cut(s)	1000
BseX3I	CGGCCG	1 cut(s)	126
BseXI	GCAGC	8 cut(s)	23, 235, 240, 328, 546, 716, 802, 805
BseYI	CCCAGC	1 cut(s)	626
Bsh1236I	CGCG	1 cut(s)	774
Bsh1285I	CGRYCG	1 cut(s)	129
BshFI	GGCC	5 cut(s)	128, 356, 471, 507, 1484
BshNI	GGYRCC	1 cut(s)	1431
BsiEI	CGRYCG	1 cut(s)	129
BsiHKAI	GWGCWC	2 cut(s)	1000, 1351
BsiHKCI	CYCGRG	1 cut(s)	275
BsiSI	CCGG	3 cut(s)	303, 620, 787
BslFI	GGGAC	1 cut(s)	434
BslI	CCNNNNNNNGG	6 cut(s)	91, 367, 658, 679, 778, 1232
BsmFI	GGGAC	1 cut(s)	434
BsmI	GAATGC	1 cut(s)	884
BsnI	GGCC	5 cut(s)	128, 356, 471, 507, 1484
BsoBI	CYCGRG	1 cut(s)	275
Bsp1286I	GDGCHC	3 cut(s)	99, 1000, 1351
Bsp13I	TCCGGA	1 cut(s)	619
Bsp143I	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
BspACI	CCGC	5 cut(s)	138, 287, 372, 679, 772
BspANI	GGCC	5 cut(s)	128, 356, 471, 507, 1484
BspCNI	CTCAG	4 cut(s)	1005, 1138, 1285, 1413
BspEI	TCCGGA	1 cut(s)	619
BspFNI	CGCG	1 cut(s)	774
BspHI	TCATGA	2 cut(s)	846, 874
BspLI	GGNNCC	4 cut(s)	96, 624, 1062, 1433
BspMAI	CTGCAG	1 cut(s)	258
BspPI	GGATC	4 cut(s)	1055, 1068, 1084, 1531
BspT107I	GGYRCC	1 cut(s)	1431
BsrDI	GCAATG	1 cut(s)	1491
BsrI	ACTGG	5 cut(s)	336, 508, 697, 731, 1485
BssECI	CCNNGG	4 cut(s)	802, 1033, 1339, 1451
BssMI	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
BssT1I	CCWWGG	3 cut(s)	802, 1033, 1451
Bst2UI	CCWGG	2 cut(s)	1113, 1341
Bst4CI	ACNGT	4 cut(s)	832, 947, 1126, 1431
Bst6I	CTCTTC	3 cut(s)	318, 534, 1505
BstAPI	GCANNNNNTGC	1 cut(s)	253
BstC8I	GCNNGC	5 cut(s)	354, 505, 681, 755, 884
BstDEI	CTNAG	5 cut(s)	345, 1013, 1146, 1293, 1421
BstENI	CCTNNNNNAGG	1 cut(s)	365
BstF5I	GGATG	4 cut(s)	54, 639, 720, 799
BstFNI	CGCG	1 cut(s)	774
BstHHI	GCGC	1 cut(s)	383
BstKTI	GATC	8 cut(s)	244, 527, 1063, 1092, 1179, 1324, 1420, 1526
BstMBI	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
BstMCI	CGRYCG	1 cut(s)	129
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 134, 215, 253, 308, 378, 387, 754, 1289
BstNI	CCWGG	2 cut(s)	1113, 1341
BstNSI	RCATGY	2 cut(s)	961, 1004
BstSCI	CCNGG	3 cut(s)	786, 1111, 1339
BstSFI	CTRYAG	1 cut(s)	254
BstSLI	GKGCMC	1 cut(s)	1000
BstUI	CGCG	1 cut(s)	774
BstV1I	GCAGC	8 cut(s)	23, 235, 240, 328, 546, 716, 802, 805
BstX2I	RGATCY	2 cut(s)	1060, 1089
BstYI	RGATCY	2 cut(s)	1060, 1089
BstZI	CGGCCG	1 cut(s)	126
BsuRI	GGCC	5 cut(s)	128, 356, 471, 507, 1484
BtgZI	GCGATG	1 cut(s)	233
BtsCI	GGATG	4 cut(s)	54, 639, 720, 799
BtsIMutI	CAGTG	1 cut(s)	724
Cac8I	GCNNGC	5 cut(s)	354, 505, 681, 755, 884
CaiI	CAGNNNCTG	2 cut(s)	253, 1544
CciI	TCATGA	2 cut(s)	846, 874
CfoI	GCGC	1 cut(s)	383
Cfr13I	GGNCC	3 cut(s)	529, 622, 799
CseI	GACGC	2 cut(s)	199, 1530
Csp6I	GTAC	1 cut(s)	1432
CspCI	CAANNNNNGTGG	2 cut(s)	1424, 1459
CviQI	GTAC	1 cut(s)	1432
DdeI	CTNAG	5 cut(s)	345, 1013, 1146, 1293, 1421
DpnI	GATC	8 cut(s)	243, 526, 1062, 1091, 1178, 1323, 1419, 1525
DpnII	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
EaeI	YGGCCR	3 cut(s)	126, 469, 505
EagI	CGGCCG	1 cut(s)	126
Eam1104I	CTCTTC	3 cut(s)	318, 534, 1505
EarI	CTCTTC	3 cut(s)	318, 534, 1505
EclXI	CGGCCG	1 cut(s)	126
Eco130I	CCWWGG	3 cut(s)	802, 1033, 1451
Eco147I	AGGCCT	1 cut(s)	356
Eco24I	GRGCYC	1 cut(s)	99
Eco47I	GGWCC	3 cut(s)	529, 622, 799
Eco52I	CGGCCG	1 cut(s)	126
Eco57I	CTGAAG	2 cut(s)	350, 425
Eco88I	CYCGRG	1 cut(s)	275
EcoNI	CCTNNNNNAGG	1 cut(s)	365
EcoO109I	RGGNCCY	1 cut(s)	799
EcoRI	GAATTC	2 cut(s)	398, 615
EcoRII	CCWGG	2 cut(s)	1111, 1339
EcoT14I	CCWWGG	3 cut(s)	802, 1033, 1451
EcoT22I	ATGCAT	2 cut(s)	598, 1233
EcoT38I	GRGCYC	1 cut(s)	99
ErhI	CCWWGG	3 cut(s)	802, 1033, 1451
FaqI	GGGAC	1 cut(s)	434
FauI	CCCGC	2 cut(s)	294, 672
FbaI	TGATCA	2 cut(s)	1176, 1321
Fnu4HI	GCNGC	9 cut(s)	12, 138, 224, 254, 342, 560, 705, 816, 819
FokI	GGATG	4 cut(s)	41, 646, 707, 806
FriOI	GRGCYC	1 cut(s)	99
Fsp4HI	GCNGC	9 cut(s)	12, 138, 224, 254, 342, 560, 705, 816, 819
FspBI	CTAG	2 cut(s)	564, 912
FspI	TGCGCA	1 cut(s)	382
GlaI	GCGC	1 cut(s)	382
GluI	GCNGC	9 cut(s)	12, 138, 224, 254, 342, 560, 705, 816, 819
GsaI	CCCAGC	1 cut(s)	630
GsuI	CTGGAG	1 cut(s)	714
HaeIII	GGCC	5 cut(s)	128, 356, 471, 507, 1484
HapII	CCGG	3 cut(s)	303, 620, 787
HgaI	GACGC	2 cut(s)	199, 1530
HhaI	GCGC	1 cut(s)	383
Hin6I	GCGC	1 cut(s)	381
HinP1I	GCGC	1 cut(s)	381
HincII	GTYRAC	2 cut(s)	835, 986
HindII	GTYRAC	2 cut(s)	835, 986
HindIII	AAGCTT	1 cut(s)	1163
HinfI	GANTC	5 cut(s)	161, 717, 825, 920, 1260
HpaII	CCGG	3 cut(s)	303, 620, 787
HphI	GGTGA	5 cut(s)	156, 386, 587, 602, 1057
Hpy166II	GTNNAC	6 cut(s)	835, 986, 998, 1259, 1397, 1427
Hpy188III	TCNNGA	8 cut(s)	230, 277, 620, 847, 875, 1325, 1398, 1475
Hpy8I	GTNNAC	6 cut(s)	835, 986, 998, 1259, 1397, 1427
HpyAV	CCTTC	9 cut(s)	156, 178, 208, 367, 371, 436, 1285, 1322, 1509
HpyCH4III	ACNGT	4 cut(s)	832, 947, 1126, 1431
HpyCH4IV	ACGT	2 cut(s)	1374, 1409
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 134, 215, 253, 308, 378, 387, 754, 1289
HpyF3I	CTNAG	5 cut(s)	345, 1013, 1146, 1293, 1421
HpySE526I	ACGT	2 cut(s)	1374, 1409
HspAI	GCGC	1 cut(s)	381
Kpn2I	TCCGGA	1 cut(s)	619
KpnI	GGTACC	1 cut(s)	1435
Ksp22I	TGATCA	2 cut(s)	1176, 1321
Kzo9I	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
LmnI	GCTCC	6 cut(s)	94, 250, 315, 1142, 1193, 1255
Lsp1109I	GCAGC	8 cut(s)	23, 235, 240, 328, 546, 716, 802, 805
LweI	GCATC	4 cut(s)	210, 583, 1047, 1270
MaeI	CTAG	2 cut(s)	564, 912
MaeII	ACGT	2 cut(s)	1374, 1409
MaeIII	GTNAC	6 cut(s)	566, 575, 1207, 1220, 1375, 1445
MalI	GATC	8 cut(s)	243, 526, 1062, 1091, 1178, 1323, 1419, 1525
MboI	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
MboII	GAAGA	7 cut(s)	223, 335, 356, 431, 551, 1128, 1522
MflI	RGATCY	2 cut(s)	1060, 1089
MhlI	GDGCHC	3 cut(s)	99, 1000, 1351
MlsI	TGGCCA	2 cut(s)	471, 507
MluCI	AATT	6 cut(s)	398, 533, 615, 730, 1128, 1203
MluNI	TGGCCA	2 cut(s)	471, 507
MlyI	GAGTC	3 cut(s)	726, 819, 1254
Mox20I	TGGCCA	2 cut(s)	471, 507
Mph1103I	ATGCAT	2 cut(s)	598, 1233
MroI	TCCGGA	1 cut(s)	619
MroXI	GAANNNNTTC	1 cut(s)	615
MscI	TGGCCA	2 cut(s)	471, 507
MseI	TTAA	1 cut(s)	486
MslI	CAYNNNNRTG	2 cut(s)	1043, 1157
Msp20I	TGGCCA	2 cut(s)	471, 507
MspI	CCGG	3 cut(s)	303, 620, 787
MspR9I	CCNGG	3 cut(s)	788, 1113, 1341
Mva1269I	GAATGC	1 cut(s)	884
MvaI	CCWGG	2 cut(s)	1113, 1341
MvnI	CGCG	1 cut(s)	774
MwoI	GCNNNNNNNGC	9 cut(s)	11, 134, 215, 253, 308, 378, 387, 754, 1289
NciI	CCSGG	1 cut(s)	788
NdeII	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
NlaIV	GGNNCC	4 cut(s)	96, 624, 1062, 1433
NmeAIII	GCCGAG	1 cut(s)	104
NmuCI	GTSAC	5 cut(s)	575, 1207, 1220, 1375, 1445
NsbI	TGCGCA	1 cut(s)	382
NsiI	ATGCAT	2 cut(s)	598, 1233
NspI	RCATGY	2 cut(s)	961, 1004
PaeR7I	CTCGAG	1 cut(s)	275
PagI	TCATGA	2 cut(s)	846, 874
PceI	AGGCCT	1 cut(s)	356
PciI	ACATGT	1 cut(s)	1000
PctI	GAATGC	1 cut(s)	884
PdmI	GAANNNNTTC	1 cut(s)	615
PfeI	GAWTC	2 cut(s)	161, 920
PflFI	GACNNNGTC	1 cut(s)	581
PflMI	CCANNNNNTGG	1 cut(s)	658
PkrI	GCNGC	9 cut(s)	13, 139, 225, 255, 343, 561, 706, 817, 820
PleI	GAGTC	3 cut(s)	725, 819, 1254
PpsI	GAGTC	3 cut(s)	725, 819, 1254
PpuMI	RGGWCCY	1 cut(s)	799
PscI	ACATGT	1 cut(s)	1000
Psp5II	RGGWCCY	1 cut(s)	799
Psp6I	CCWGG	2 cut(s)	1111, 1339
PspFI	CCCAGC	1 cut(s)	626
PspGI	CCWGG	2 cut(s)	1111, 1339
PspN4I	GGNNCC	4 cut(s)	96, 624, 1062, 1433
PspPI	GGNCC	3 cut(s)	529, 622, 799
PspPPI	RGGWCCY	1 cut(s)	799
PstI	CTGCAG	1 cut(s)	258
PstNI	CAGNNNCTG	2 cut(s)	253, 1544
PsuI	RGATCY	2 cut(s)	1060, 1089
PsyI	GACNNNGTC	1 cut(s)	581
RsaI	GTAC	1 cut(s)	1433
RsaNI	GTAC	1 cut(s)	1432
RseI	CAYNNNNRTG	2 cut(s)	1043, 1157
SaqAI	TTAA	1 cut(s)	486
SatI	GCNGC	9 cut(s)	12, 138, 224, 254, 342, 560, 705, 816, 819
Sau3AI	GATC	8 cut(s)	241, 524, 1060, 1089, 1176, 1321, 1417, 1523
Sau96I	GGNCC	3 cut(s)	529, 622, 799
SchI	GAGTC	3 cut(s)	726, 819, 1254
ScrFI	CCNGG	3 cut(s)	788, 1113, 1341
SduI	GDGCHC	3 cut(s)	99, 1000, 1351
SfaNI	GCATC	4 cut(s)	210, 583, 1047, 1270
SfcI	CTRYAG	1 cut(s)	254
Sfr274I	CTCGAG	1 cut(s)	275
SinI	GGWCC	3 cut(s)	529, 622, 799
SlaI	CTCGAG	1 cut(s)	275
SmiMI	CAYNNNNRTG	2 cut(s)	1043, 1157
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
Sse9I	AATT	6 cut(s)	398, 533, 615, 730, 1128, 1203
SseBI	AGGCCT	1 cut(s)	356
SsiI	CCGC	5 cut(s)	138, 287, 372, 679, 772
SspMI	CTAG	2 cut(s)	564, 912
StuI	AGGCCT	1 cut(s)	356
StyD4I	CCNGG	3 cut(s)	786, 1111, 1339
StyI	CCWWGG	3 cut(s)	802, 1033, 1451
TaaI	ACNGT	4 cut(s)	832, 947, 1126, 1431
TaiI	ACGT	2 cut(s)	1377, 1412
TaqI	TCGA	4 cut(s)	276, 990, 1278, 1526
TasI	AATT	6 cut(s)	398, 533, 615, 730, 1128, 1203
TauI	GCSGC	1 cut(s)	140
TfiI	GAWTC	2 cut(s)	161, 920
Tru1I	TTAA	1 cut(s)	486
Tru9I	TTAA	1 cut(s)	486
TscAI	CASTG	1 cut(s)	731
TseFI	GTSAC	5 cut(s)	575, 1207, 1220, 1375, 1445
TseI	GCWGC	8 cut(s)	11, 223, 253, 341, 559, 704, 815, 818
Tsp45I	GTSAC	5 cut(s)	575, 1207, 1220, 1375, 1445
TspDTI	ATGAA	4 cut(s)	618, 891, 912, 1083
TspGWI	ACGGA	4 cut(s)	419, 1242, 1394, 1522
TspRI	CASTG	1 cut(s)	731
Tth111I	GACNNNGTC	1 cut(s)	581
Van91I	CCANNNNNTGG	1 cut(s)	658
VneI	GTGCAC	1 cut(s)	996
VpaK11BI	GGWCC	3 cut(s)	529, 622, 799
XagI	CCTNNNNNAGG	1 cut(s)	365
XapI	RAATTY	3 cut(s)	398, 615, 730
XceI	RCATGY	2 cut(s)	961, 1004
XcmI	CCANNNNNNNNNTGG	2 cut(s)	183, 190
XhoI	CTCGAG	1 cut(s)	275
XmnI	GAANNNNTTC	1 cut(s)	615
XspI	CTAG	2 cut(s)	564, 912
Zsp2I	ATGCAT	2 cut(s)	598, 1233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.