Rroxscaffold_3G00251990

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012776
Physical Location & Seq
Forward (+)
45968383 .. 45972205
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251990.1

Sequence Viewer

Length: 1437 bp
ATGTCTCTGACAGCCACAGCCGCCGCCTCAGCAGCAGGCTCTGCTAATCTCACCACCCCAAACCCCTTCCTCTCCCGTACCACCACCCCAAAACCCACGACCCATTTCAAACTCTTCACTCTCCCCAAAAACCCCAAACCCACAATTTCCGCCGTCTCCTCCTCCCCTTCCGCAATTTCCTCCACCGCCGGGCCCTCCGACTGGGCCGTGGACTCATGGAAATCCAGGACGGCCCATCAGCTCCCGGTCTACCCGGACCCGGACGAGCTCCGGACCGTGTTGACCACCCTCGAGACCTTCCCGCCAATTGTGTTTGCCGGAGAGGCGAGGAGGCTGGAGACCTTGCTCGGCAAGGCGGCTCAAGGCGAGGCCTTTTTGCTTCAGGGAGGTGATTGCGCCGAGAGTTTCAAGGAGTTTAATGCTAATAACATTAGGGATACCTTCAGAGTCTTCTTGCAGATGGCCATCACTCTCATCTATGGGGCCCAAATGCCCGTCATCAAGGTTGGGAGAATGGCAGGCCAATTCGCAAAGCCTAGGTCTGATAATTTTGAGACTAGAGATGGTGTGAAGCTCCCAAGTTATCGGGGAGACAATATCAATGGTGATGCTTTTGATGAGAAATCTAGAAGACCAGACCCACAAAGGTTACTGAGAGCATACCTCCAATCTGTTGGCACACTGAATCTTCTTAGAGCATTTGCCACTGGTGGTTATGCTGCAATGCAGAGAGTCTCAGATTGGAATCTTGACTTCGTAGAGCATAGCGAGCAAGCGAACAGGGAGGATTCCACAACTGGCCGTTATTATGATTGTTCTGCCCACATGCTTTGGGTAGGGGAGAGGACTCGACAATTGGATGGGGCACATGTTGAATTCCTTCGTGGTGTATCCAATCCTCTTGGCATTAAGGTGAGTGATAAGATGGACCCAAATGAGCTTGTGAAACTGTGTGAGATTCTTAATCCTCACAACAGACCTGGTAGGCTGACAATAATTACCAGGATGGGAGCAGATAATATGCGGATAAAGCTCCCCCATTTGATCAGAGCTGTGCGCCAGGCAGGGCTTATTGTTACATGGGTCAGTGATCCCATGCATGGGAACACCATAAAGGCTCCATGTGGGCTCAAGACACGGCCATTTGATGCAATCAGGGCTGAATTGAGAGCCTTCTTTGACGTCCATGACCAAGAAGGGAGCTACCCTGGAGGAGTGCATCTGGAGATGACTGGGCAGAATGTGACAGAGTGCGTTGGAGGGTCAAAGACTGTGACCTTCAGTGACTTGAACTCCCGTTATCACACGCATTGTGACCCCAGACTGAATGCATCGCAGTCGCTTGAGTTGGCCTTTTTAATATCAGATAGGCTGAGGAAAGACAGGTTAAAATCATCTAGGACTGATTATCAGATGAATGGCAGTGCTCGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00400 ko01100 ko01110 ko01130 ko01230 ko02024 map00400 map01100 map01110 map01130 map01230 map02024
Pfam Domains
Protein Families
DAHP_synth_2

Protein Analysis

478

Amino Acids

52.88

Weight (kDa)

9.13

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 68 - 260 2.6e-80 Class-II DAHP synthetase family
DAHP_synth_2 PF01474 259 - 455 3.5e-96 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012776)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33510
fragaria_vesca FvH4_5g20460
malus_domestica MD04G1055100.v1.1 MD05G1042700.v1.1
prunus_persica Prupe.5G055000_v2.0.a1 Prupe.8G064200_v2.0.a1
pyrus_communis pycom05g03530
rosa_chinensis RchiOBHm_Chr7g0205781
rosa_laevigata RLG00000003355
rosa_multiflora Rmu_co8369421.1_g000001 Rmu_sc0000076.1_g000051 Rmu_sc0008518.1_g000012
rosa_roxburghii Rroxscaffold_3G00251990
rosa_rugosa Rorug07G0093000
rosa_samantha Rh7AG224500 Rh7CG238100 Rh7DG231200
rosa_wichuraiana Rw7G019360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1185
AccI GTMKAC 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 270
AciI CCGC 8 cut(s) 21, 24, 150, 171, 186, 302, 356, 1024
AclWI GGATC 1 cut(s) 1085
AcoI YGGCCR 3 cut(s) 462, 799, 1139
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 3 cut(s) 365, 427, 1264
AcyI GRCGYC 1 cut(s) 1182
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 5 cut(s) 189, 201, 259, 1100, 1101
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 4 cut(s) 109, 409, 875, 1291
AjnI CCWGG 5 cut(s) 224, 979, 1001, 1059, 1207
AluBI AGCT 7 cut(s) 241, 268, 574, 940, 1033, 1052, 1203
AluI AGCT 7 cut(s) 241, 268, 574, 940, 1033, 1052, 1203
Alw21I GWGCWC 2 cut(s) 270, 1429
Alw26I GTCTC 7 cut(s) 9, 160, 287, 332, 548, 585, 739
AlwI GGATC 1 cut(s) 1085
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 2 cut(s) 290, 1428
Aor13HI TCCGGA 1 cut(s) 270
ApaI GGGCCC 2 cut(s) 195, 487
ApeKI GCWGC 2 cut(s) 32, 719
ApoI RAATTY 1 cut(s) 875
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
Asp700I GAANNNNTTC 1 cut(s) 879
AspA2I CCTAGG 1 cut(s) 536
AspLEI GCGC 2 cut(s) 398, 1059
AspS9I GGNCC 9 cut(s) 191, 192, 204, 232, 256, 273, 483, 484, 928
AsuC2I CCSGG 4 cut(s) 190, 245, 254, 260
AsuHPI GGTGA 4 cut(s) 43, 401, 617, 925
AvaI CYCGRG 2 cut(s) 290, 1428
AvaII GGWCC 3 cut(s) 256, 273, 928
AvrII CCTAGG 1 cut(s) 536
BaeGI GKGCMC 3 cut(s) 195, 487, 868
BalI TGGCCA 1 cut(s) 464
BanII GRGCYC 4 cut(s) 195, 270, 487, 1131
BbsI GAAGAC 2 cut(s) 442, 637
Bbv12I GWGCWC 2 cut(s) 270, 1429
BbvCI CCTCAGC 2 cut(s) 28, 1373
BbvI GCAGC 2 cut(s) 44, 706
BccI CCATC 7 cut(s) 243, 454, 473, 557, 854, 919, 1000
BceAI ACGGC 5 cut(s) 137, 191, 246, 786, 1154
BcgI CGANNNNNNTGC 2 cut(s) 1320, 1354
BciT130I CCWGG 5 cut(s) 226, 981, 1003, 1061, 1209
BciVI GTATCC 2 cut(s) 430, 901
BclI TGATCA 1 cut(s) 1044
BcnI CCSGG 4 cut(s) 190, 245, 254, 260
BcoDI GTCTC 7 cut(s) 9, 160, 287, 332, 548, 585, 739
BfaI CTAG 4 cut(s) 537, 558, 627, 1398
BfuI GTATCC 2 cut(s) 430, 901
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 5 cut(s) 21, 24, 33, 357, 720
BlnI CCTAGG 1 cut(s) 536
BlsI GCNGC 5 cut(s) 22, 25, 34, 358, 721
Bme1390I CCNGG 9 cut(s) 190, 226, 245, 254, 260, 981, 1003, 1061, 1209
Bme18I GGWCC 3 cut(s) 256, 273, 928
BmeT110I CYCGRG 2 cut(s) 290, 1428
BmgT120I GGNCC 9 cut(s) 191, 192, 204, 232, 256, 273, 483, 484, 928
BmiI GGNNCC 6 cut(s) 193, 258, 484, 485, 930, 1119
BmrFI CCNGG 9 cut(s) 190, 226, 245, 254, 260, 981, 1003, 1061, 1209
BmrI ACTGGG 2 cut(s) 211, 1242
BmsI GCATC 4 cut(s) 598, 1138, 1228, 1340
BmuI ACTGGG 2 cut(s) 211, 1242
BpiI GAAGAC 2 cut(s) 442, 637
BplI GAGNNNNNCTC 2 cut(s) 648, 680
BpmI CTGGAG 3 cut(s) 356, 1230, 1244
Bpu10I CCTNAGC 2 cut(s) 28, 1373
BpuEI CTTGAG 3 cut(s) 345, 1115, 1364
BpuMI CCSGG 4 cut(s) 190, 245, 254, 260
BsaBI GATNNNNATC 2 cut(s) 464, 744
BsaHI GRCGYC 1 cut(s) 1182
BsaI GGTCTC 2 cut(s) 287, 332
BsaJI CCNNGG 3 cut(s) 207, 536, 1207
BsaWI WCCGGW 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 1277, 1307
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 201, 259, 1100, 1101
Bse1I ACTGG 4 cut(s) 206, 712, 802, 1237
Bse3DI GCAATG 1 cut(s) 729
Bse8I GATNNNNATC 2 cut(s) 464, 744
BseAI TCCGGA 1 cut(s) 270
BseBI CCWGG 5 cut(s) 226, 981, 1003, 1061, 1209
BseDI CCNNGG 3 cut(s) 207, 536, 1207
BseGI GGATG 2 cut(s) 865, 1011
BseJI GATNNNNATC 2 cut(s) 464, 744
BseLI CCNNNNNNNGG 5 cut(s) 189, 201, 259, 1100, 1101
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 4 cut(s) 42, 644, 750, 1364
BseNI ACTGG 4 cut(s) 206, 712, 802, 1237
BseRI GAGGAG 4 cut(s) 148, 151, 343, 1227
BseSI GKGCMC 3 cut(s) 195, 487, 868
BseXI GCAGC 2 cut(s) 44, 706
BsiHKAI GWGCWC 2 cut(s) 270, 1429
BsiHKCI CYCGRG 2 cut(s) 290, 1428
BsiSI CCGG 6 cut(s) 189, 245, 254, 260, 271, 318
BslI CCNNNNNNNGG 5 cut(s) 189, 201, 259, 1100, 1101
BsmAI GTCTC 7 cut(s) 9, 160, 287, 332, 548, 585, 739
BsmBI CGTCTC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 1333
Bso31I GGTCTC 2 cut(s) 287, 332
BsoBI CYCGRG 2 cut(s) 290, 1428
Bsp120I GGGCCC 2 cut(s) 191, 483
Bsp1286I GDGCHC 6 cut(s) 195, 270, 487, 868, 1131, 1429
Bsp13I TCCGGA 1 cut(s) 270
Bsp143I GATC 2 cut(s) 1044, 1090
BspACI CCGC 8 cut(s) 21, 24, 150, 171, 186, 302, 356, 1024
BspCNI CTCAG 4 cut(s) 41, 645, 749, 1365
BspEI TCCGGA 1 cut(s) 270
BspLI GGNNCC 6 cut(s) 193, 258, 484, 485, 930, 1119
BspPI GGATC 1 cut(s) 1085
BspTNI GGTCTC 2 cut(s) 287, 332
BsrDI GCAATG 1 cut(s) 729
BsrI ACTGG 4 cut(s) 206, 712, 802, 1237
BssECI CCNNGG 3 cut(s) 207, 536, 1207
BssMI GATC 2 cut(s) 1044, 1090
BssNI GRCGYC 1 cut(s) 1182
BssT1I CCWWGG 1 cut(s) 536
Bst2UI CCWGG 5 cut(s) 226, 981, 1003, 1061, 1209
Bst4CI ACNGT 3 cut(s) 277, 951, 1273
Bst6I CTCTTC 1 cut(s) 119
BstACI GRCGYC 1 cut(s) 1182
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 37, 520, 770, 774
BstDEI CTNAG 5 cut(s) 28, 653, 692, 736, 1373
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 865, 1011
BstHHI GCGC 2 cut(s) 398, 1059
BstKTI GATC 2 cut(s) 1047, 1093
BstMAI GTCTC 7 cut(s) 9, 160, 287, 332, 548, 585, 739
BstMBI GATC 2 cut(s) 1044, 1090
BstMWI GCNNNNNNNGC 8 cut(s) 20, 29, 32, 41, 323, 769, 1030, 1157
BstNI CCWGG 5 cut(s) 226, 981, 1003, 1061, 1209
BstNSI RCATGY 2 cut(s) 829, 872
BstSCI CCNGG 9 cut(s) 188, 224, 243, 252, 258, 979, 1001, 1059, 1207
BstSLI GKGCMC 3 cut(s) 195, 487, 868
BstV1I GCAGC 2 cut(s) 44, 706
BstV2I GAAGAC 2 cut(s) 442, 637
BstXI CCANNNNNNTGG 1 cut(s) 674
BsuI GTATCC 2 cut(s) 430, 901
BtgI CCRYGG 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 1317
BtsCI GGATG 2 cut(s) 865, 1011
BtsI GCAGTG 1 cut(s) 1429
BtsIMutI CAGTG 5 cut(s) 680, 705, 1093, 1288, 1429
Cac8I GCNNGC 4 cut(s) 37, 520, 770, 774
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 2 cut(s) 398, 1059
Cfr13I GGNCC 9 cut(s) 191, 192, 204, 232, 256, 273, 483, 484, 928
CpoI CGGWCCG 1 cut(s) 273
CsiI ACCWGGT 1 cut(s) 979
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 812, 847
CspI CGGWCCG 1 cut(s) 273
CviAII CATG 8 cut(s) 216, 826, 869, 1080, 1096, 1100, 1122, 1187
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 5 cut(s) 28, 653, 692, 736, 1373
DpnI GATC 2 cut(s) 1046, 1092
DpnII GATC 2 cut(s) 1044, 1090
EaeI YGGCCR 3 cut(s) 462, 799, 1139
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 536
Eco147I AGGCCT 1 cut(s) 371
Eco24I GRGCYC 4 cut(s) 195, 270, 487, 1131
Eco31I GGTCTC 2 cut(s) 287, 332
Eco47I GGWCC 3 cut(s) 256, 273, 928
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 3 cut(s) 365, 427, 1264
Eco88I CYCGRG 2 cut(s) 290, 1428
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 2 cut(s) 192, 483
EcoRI GAATTC 1 cut(s) 875
EcoRII CCWGG 5 cut(s) 224, 979, 1001, 1059, 1207
EcoT14I CCWWGG 1 cut(s) 536
EcoT22I ATGCAT 2 cut(s) 1101, 1333
EcoT38I GRGCYC 4 cut(s) 195, 270, 487, 1131
ErhI CCWWGG 1 cut(s) 536
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 8 cut(s) 219, 829, 872, 1083, 1099, 1103, 1125, 1190
FatI CATG 8 cut(s) 215, 825, 868, 1079, 1095, 1099, 1121, 1186
FauI CCCGC 1 cut(s) 309
FbaI TGATCA 1 cut(s) 1044
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 21, 24, 33, 357, 720
FokI GGATG 2 cut(s) 872, 1018
FriOI GRGCYC 4 cut(s) 195, 270, 487, 1131
Fsp4HI GCNGC 5 cut(s) 21, 24, 33, 357, 720
FspBI CTAG 4 cut(s) 537, 558, 627, 1398
GlaI GCGC 2 cut(s) 397, 1058
GluI GCNGC 5 cut(s) 21, 24, 33, 357, 720
GsuI CTGGAG 3 cut(s) 356, 1230, 1244
HapII CCGG 6 cut(s) 189, 245, 254, 260, 271, 318
HhaI GCGC 2 cut(s) 398, 1059
Hin1I GRCGYC 1 cut(s) 1182
Hin1II CATG 8 cut(s) 219, 829, 872, 1083, 1099, 1103, 1125, 1190
Hin6I GCGC 2 cut(s) 396, 1057
HinP1I GCGC 2 cut(s) 396, 1057
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 8 cut(s) 212, 447, 685, 732, 745, 788, 847, 958
HpaII CCGG 6 cut(s) 189, 245, 254, 260, 271, 318
HphI GGTGA 4 cut(s) 43, 401, 617, 925
Hpy166II GTNNAC 3 cut(s) 211, 250, 282
Hpy188I TCNGA 8 cut(s) 9, 199, 446, 544, 739, 1049, 1366, 1413
Hpy188III TCNNGA 6 cut(s) 271, 292, 627, 749, 1132, 1223
Hpy8I GTNNAC 3 cut(s) 211, 250, 282
HpyAV CCTTC 8 cut(s) 76, 177, 307, 451, 890, 1183, 1190, 1288
HpyCH4III ACNGT 3 cut(s) 277, 951, 1273
HpyCH4IV ACGT 1 cut(s) 1182
HpyCH4V TGCA 7 cut(s) 457, 722, 727, 1099, 1151, 1219, 1331
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 29, 32, 41, 323, 769, 1030, 1157
HpyF3I CTNAG 5 cut(s) 28, 653, 692, 736, 1373
HpySE526I ACGT 1 cut(s) 1182
Hsp92I GRCGYC 1 cut(s) 1182
Hsp92II CATG 8 cut(s) 219, 829, 872, 1083, 1099, 1103, 1125, 1190
HspAI GCGC 2 cut(s) 396, 1057
Kpn2I TCCGGA 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 1044
Kzo9I GATC 2 cut(s) 1044, 1090
LmnI GCTCC 7 cut(s) 246, 273, 579, 1010, 1038, 1123, 1200
Lsp1109I GCAGC 2 cut(s) 44, 706
LweI GCATC 4 cut(s) 598, 1138, 1228, 1340
MabI ACCWGGT 1 cut(s) 979
MaeI CTAG 4 cut(s) 537, 558, 627, 1398
MaeII ACGT 1 cut(s) 1182
MaeIII GTNAC 6 cut(s) 648, 1075, 1243, 1273, 1283, 1313
MalI GATC 2 cut(s) 1046, 1092
MboI GATC 2 cut(s) 1044, 1090
MboII GAAGA 4 cut(s) 106, 442, 642, 680
MfeI CAATTG 2 cut(s) 306, 854
MhlI GDGCHC 6 cut(s) 195, 270, 487, 868, 1131, 1429
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 9 cut(s) 144, 174, 306, 524, 547, 854, 875, 996, 1163
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 4 cut(s) 206, 456, 741, 841
MmeI TCCRAC 2 cut(s) 222, 1237
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 2 cut(s) 1101, 1333
MroI TCCGGA 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 879
MscI TGGCCA 1 cut(s) 464
MseI TTAA 5 cut(s) 417, 909, 963, 1358, 1388
MslI CAYNNNNRTG 1 cut(s) 911
Msp20I TGGCCA 1 cut(s) 464
MspI CCGG 6 cut(s) 189, 245, 254, 260, 271, 318
MspR9I CCNGG 9 cut(s) 190, 226, 245, 254, 260, 981, 1003, 1061, 1209
MunI CAATTG 2 cut(s) 306, 854
Mva1269I GAATGC 1 cut(s) 1333
MvaI CCWGG 5 cut(s) 226, 981, 1003, 1061, 1209
MwoI GCNNNNNNNGC 8 cut(s) 20, 29, 32, 41, 323, 769, 1030, 1157
NciI CCSGG 4 cut(s) 190, 245, 254, 260
NdeII GATC 2 cut(s) 1044, 1090
NlaIII CATG 8 cut(s) 219, 829, 872, 1083, 1099, 1103, 1125, 1190
NlaIV GGNNCC 6 cut(s) 193, 258, 484, 485, 930, 1119
NmeAIII GCCGAG 2 cut(s) 327, 424
NmuCI GTSAC 4 cut(s) 1243, 1273, 1283, 1313
NsiI ATGCAT 2 cut(s) 1101, 1333
NspI RCATGY 2 cut(s) 829, 872
PaeR7I CTCGAG 2 cut(s) 290, 1428
PceI AGGCCT 1 cut(s) 371
PciI ACATGT 1 cut(s) 868
PctI GAATGC 1 cut(s) 1333
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 4 cut(s) 685, 745, 788, 958
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 5 cut(s) 22, 25, 34, 358, 721
PleI GAGTC 4 cut(s) 206, 455, 740, 841
PpsI GAGTC 4 cut(s) 206, 455, 740, 841
PscI ACATGT 1 cut(s) 868
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 5 cut(s) 224, 979, 1001, 1059, 1207
PspGI CCWGG 5 cut(s) 224, 979, 1001, 1059, 1207
PspN4I GGNNCC 6 cut(s) 193, 258, 484, 485, 930, 1119
PspOMI GGGCCC 2 cut(s) 191, 483
PspPI GGNCC 9 cut(s) 191, 192, 204, 232, 256, 273, 483, 484, 928
PspXI VCTCGAGB 1 cut(s) 1428
PstNI CAGNNNCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 911
Rsr2I CGGWCCG 1 cut(s) 273
RsrII CGGWCCG 1 cut(s) 273
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 5 cut(s) 417, 909, 963, 1358, 1388
SatI GCNGC 5 cut(s) 21, 24, 33, 357, 720
Sau3AI GATC 2 cut(s) 1044, 1090
Sau96I GGNCC 9 cut(s) 191, 192, 204, 232, 256, 273, 483, 484, 928
SchI GAGTC 4 cut(s) 206, 456, 741, 841
ScrFI CCNGG 9 cut(s) 190, 226, 245, 254, 260, 981, 1003, 1061, 1209
SduI GDGCHC 6 cut(s) 195, 270, 487, 868, 1131, 1429
SexAI ACCWGGT 1 cut(s) 979
SfaNI GCATC 4 cut(s) 598, 1138, 1228, 1340
Sfr274I CTCGAG 2 cut(s) 290, 1428
SinI GGWCC 3 cut(s) 256, 273, 928
SlaI CTCGAG 2 cut(s) 290, 1428
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 5 cut(s) 290, 360, 1130, 1343, 1428
SmoI CTYRAG 5 cut(s) 290, 360, 1130, 1343, 1428
Sse9I AATT 9 cut(s) 144, 174, 306, 524, 547, 854, 875, 996, 1163
SseBI AGGCCT 1 cut(s) 371
SsiI CCGC 8 cut(s) 21, 24, 150, 171, 186, 302, 356, 1024
SspMI CTAG 4 cut(s) 537, 558, 627, 1398
SstI GAGCTC 1 cut(s) 270
StuI AGGCCT 1 cut(s) 371
StyD4I CCNGG 9 cut(s) 188, 224, 243, 252, 258, 979, 1001, 1059, 1207
StyI CCWWGG 1 cut(s) 536
TaaI ACNGT 3 cut(s) 277, 951, 1273
TaiI ACGT 1 cut(s) 1185
TaqI TCGA 3 cut(s) 291, 850, 1429
TasI AATT 9 cut(s) 144, 174, 306, 524, 547, 854, 875, 996, 1163
TauI GCSGC 3 cut(s) 23, 26, 359
TfiI GAWTC 4 cut(s) 685, 745, 788, 958
Tru1I TTAA 5 cut(s) 417, 909, 963, 1358, 1388
Tru9I TTAA 5 cut(s) 417, 909, 963, 1358, 1388
TscAI CASTG 5 cut(s) 687, 712, 1093, 1288, 1429
TseFI GTSAC 4 cut(s) 1243, 1273, 1283, 1313
TseI GCWGC 2 cut(s) 32, 719
Tsp45I GTSAC 4 cut(s) 1243, 1273, 1283, 1313
TspDTI ATGAA 1 cut(s) 1430
TspRI CASTG 5 cut(s) 687, 712, 1093, 1288, 1429
VpaK11BI GGWCC 3 cut(s) 256, 273, 928
XapI RAATTY 1 cut(s) 875
XbaI TCTAGA 1 cut(s) 626
XceI RCATGY 2 cut(s) 829, 872
XhoI CTCGAG 2 cut(s) 290, 1428
XmaJI CCTAGG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 4 cut(s) 537, 558, 627, 1398
ZraI GACGTC 1 cut(s) 1183
Zsp2I ATGCAT 2 cut(s) 1101, 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.