pycom05g04480

Protein BASIC PENTACYSTEINE4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005753
Physical Location & Seq
Reverse (-)
5276296 .. 5277327
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGATGATGGTCGGCAACATGAAAATGGGAGACACAAGATGGACTATTACAGAGGAGGGACAGCACCTCCGTGGAATATTATGGGCCAACAGCAAGCGAAGGAACCAAATGCCTTAGTCATGAACAAGAAGATCATGTCCATCATTGCTGAAAGGGATGCTGCTATTCGAGAACGGAATGCAGCACTTGCTGAAAAGAATGAAGCCTTGGCTGGAAGAGATGAGGCTCTTCGTCAGCGGGATGAGGCACTTGCTCAGCGTGATAGTGCCATGATGGAACGGGACAACGCCTATGCAGCCCTTCATTCACGGGATAATGCTGTCAACTTCCCATTGGGTGGTGGGGCTCCACGTGGAGCTAAACGCATGCACCGTCCCTCAAACCATTCAGTTACCCTGGCTGATGCTCATTACAGCACAAAAGATATGCATATAACTGAGGCCTACCCAGTTTCAGTTATATCTACTGAAGATGTCAAGTCACGTCAGACAAAGCGAGCAAAGGAGAACAAGGCATCCAGGGCAAGGCAGTCACGGAAGAAAGTAGGTGAAGATTTAAATAGGCAGGCTTCCTCGGATGGGATAAAGTATAGATCAGAGTGGGATGCTCATGATTTGGGTTTGAATCTTGTCACTTTTGACGAGTCTGCAATGCCAGTGCCAGTGTGTTCATGCACTGGAATTCCACGGCAGTGCTACAAATGGGGGAATGGCGGGTGGCAGTCATCTTGTTGCACCACCCATATGTCGATGTATCCACTACCTCAGATGCCAAATAAGCGCCATGCCCGGATGGGTGGGCGGAAGATGAGCGGAAATGTTTTTACTAGATTGCTTAGTCGGCTAGCAGCAGATGGTCATGATTTGTCAATACCGCTGGATTTGAAAGAATATTGGTCCAGACATGGGACAAATCGCTACATAACGATCAAGTAG

Protein Analysis

312

Amino Acids

34.99

Weight (kDa)

9.58

Isoelectric Point (pI)

60.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAGA_bind PF06217 1 - 311 1.5e-113 GAGA binding protein-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 338
AccBSI CCGCTC 1 cut(s) 813
AciI CCGC 5 cut(s) 238, 714, 802, 813, 875
AcsI RAATTY 1 cut(s) 681
AcuI CTGAAG 1 cut(s) 489
AcvI CACGTG 1 cut(s) 353
AfiI CCNNNNNNNGG 4 cut(s) 338, 525, 579, 906
AgsI TTSAA 2 cut(s) 625, 886
AjiI CACGTC 1 cut(s) 485
AjnI CCWGG 2 cut(s) 396, 518
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AleI CACNNNNGTG 2 cut(s) 70, 691
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
Alw26I GTCTC 1 cut(s) 25
AoxI GGCC 2 cut(s) 85, 441
ApeKI GCWGC 4 cut(s) 161, 182, 296, 848
ApoI RAATTY 1 cut(s) 681
AspLEI GCGC 1 cut(s) 783
AspS9I GGNCC 2 cut(s) 85, 897
AsuC2I CCSGG 1 cut(s) 790
AsuHPI GGTGA 1 cut(s) 560
AsuNHI GCTAGC 1 cut(s) 844
AvaII GGWCC 1 cut(s) 897
BanII GRGCYC 1 cut(s) 349
BbrPI CACGTG 1 cut(s) 353
BbvI GCAGC 4 cut(s) 148, 194, 308, 860
BccI CCATC 7 cut(s) 2, 34, 149, 268, 572, 787, 848
BceAI ACGGC 1 cut(s) 704
BciT130I CCWGG 2 cut(s) 398, 520
BciVI GTATCC 1 cut(s) 765
BcnI CCSGG 1 cut(s) 790
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 828, 845
BfoI RGCGCY 1 cut(s) 784
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 4 cut(s) 162, 183, 297, 849
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 4 cut(s) 163, 184, 298, 850
Bme1390I CCNGG 3 cut(s) 398, 520, 790
Bme18I GGWCC 1 cut(s) 897
BmgBI CACGTC 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 85, 897
BmiI GGNNCC 2 cut(s) 105, 348
BmrFI CCNGG 3 cut(s) 398, 520, 790
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 5 cut(s) 148, 394, 524, 595, 759
BmtI GCTAGC 1 cut(s) 848
BmuI ACTGGG 1 cut(s) 443
Bpu1102I GCTNAGC 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 790
BsaAI YACGTR 1 cut(s) 353
BsaJI CCNNGG 6 cut(s) 71, 207, 396, 519, 573, 686
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 525, 579, 906
Bse1I ACTGG 4 cut(s) 449, 656, 662, 682
Bse3DI GCAATG 2 cut(s) 144, 657
BseBI CCWGG 2 cut(s) 398, 520
BseDI CCNNGG 6 cut(s) 71, 207, 396, 519, 573, 686
BseGI GGATG 7 cut(s) 10, 163, 247, 515, 583, 610, 798
BseLI CCNNNNNNNGG 4 cut(s) 338, 525, 579, 906
BseMI GCAATG 2 cut(s) 144, 657
BseMII CTCAG 3 cut(s) 269, 429, 779
BseNI ACTGG 4 cut(s) 449, 656, 662, 682
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 4 cut(s) 148, 194, 308, 860
BshFI GGCC 2 cut(s) 87, 443
BsiSI CCGG 1 cut(s) 790
BslFI GGGAC 4 cut(s) 73, 296, 360, 922
BslI CCNNNNNNNGG 4 cut(s) 338, 525, 579, 906
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 4 cut(s) 73, 296, 360, 922
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 2 cut(s) 87, 443
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 3 cut(s) 132, 593, 927
Bsp1720I GCTNAGC 1 cut(s) 255
BspACI CCGC 5 cut(s) 238, 714, 802, 813, 875
BspANI GGCC 2 cut(s) 87, 443
BspCNI CTCAG 3 cut(s) 268, 430, 778
BspHI TCATGA 3 cut(s) 120, 610, 859
BspLI GGNNCC 2 cut(s) 105, 348
BspOI GCTAGC 1 cut(s) 848
BspQI GCTCTTC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 813
BsrDI GCAATG 2 cut(s) 144, 657
BsrI ACTGG 4 cut(s) 449, 656, 662, 682
BssECI CCNNGG 6 cut(s) 71, 207, 396, 519, 573, 686
BssMI GATC 3 cut(s) 132, 593, 927
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 398, 520
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 2 cut(s) 211, 234
BstAPI GCANNNNNTGC 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 353
BstC8I GCNNGC 5 cut(s) 96, 368, 498, 567, 846
BstDEI CTNAG 5 cut(s) 115, 255, 438, 765, 836
BstDSI CCRYGG 2 cut(s) 71, 686
BstF5I GGATG 7 cut(s) 10, 163, 247, 515, 583, 610, 798
BstH2I RGCGCY 1 cut(s) 784
BstHHI GCGC 1 cut(s) 783
BstKTI GATC 3 cut(s) 135, 596, 930
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 3 cut(s) 132, 593, 927
BstMWI GCNNNNNNNGC 5 cut(s) 188, 296, 521, 778, 841
BstNI CCWGG 2 cut(s) 398, 520
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 3 cut(s) 396, 518, 788
BstV1I GCAGC 4 cut(s) 148, 194, 308, 860
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 2 cut(s) 87, 443
BtgI CCRYGG 2 cut(s) 71, 686
BtrI CACGTC 1 cut(s) 485
BtsCI GGATG 7 cut(s) 10, 163, 247, 515, 583, 610, 798
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 4 cut(s) 663, 669, 675, 698
Cac8I GCNNGC 5 cut(s) 96, 368, 498, 567, 846
CciI TCATGA 3 cut(s) 120, 610, 859
CfoI GCGC 1 cut(s) 783
Cfr13I GGNCC 2 cut(s) 85, 897
DdeI CTNAG 5 cut(s) 115, 255, 438, 765, 836
DpnI GATC 3 cut(s) 134, 595, 929
DpnII GATC 3 cut(s) 132, 593, 927
DraI TTTAAA 1 cut(s) 558
Eam1104I CTCTTC 2 cut(s) 211, 234
EarI CTCTTC 2 cut(s) 211, 234
EciI GGCGGA 1 cut(s) 817
Eco130I CCWWGG 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 897
Eco57I CTGAAG 1 cut(s) 489
Eco72I CACGTG 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 681
EcoRII CCWGG 2 cut(s) 396, 518
EcoT14I CCWWGG 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 207
FaqI GGGAC 4 cut(s) 73, 296, 360, 922
FauI CCCGC 2 cut(s) 231, 707
FauNDI CATATG 1 cut(s) 744
Fnu4HI GCNGC 4 cut(s) 162, 183, 297, 849
FokI GGATG 7 cut(s) 17, 170, 254, 502, 590, 617, 805
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 4 cut(s) 162, 183, 297, 849
FspBI CTAG 2 cut(s) 828, 845
GlaI GCGC 1 cut(s) 782
GluI GCNGC 4 cut(s) 162, 183, 297, 849
HaeII RGCGCY 1 cut(s) 784
HaeIII GGCC 2 cut(s) 87, 443
HapII CCGG 1 cut(s) 790
HhaI GCGC 1 cut(s) 783
Hin6I GCGC 1 cut(s) 781
HinP1I GCGC 1 cut(s) 781
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 2 cut(s) 625, 644
HpaII CCGG 1 cut(s) 790
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 4 cut(s) 489, 577, 598, 768
Hpy188III TCNNGA 5 cut(s) 121, 170, 611, 860, 900
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 94, 311
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 2 cut(s) 352, 484
HpyCH4V TGCA 7 cut(s) 182, 296, 370, 430, 650, 675, 735
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 296, 521, 778, 841
HpyF3I CTNAG 5 cut(s) 115, 255, 438, 765, 836
HpySE526I ACGT 2 cut(s) 352, 484
HspAI GCGC 1 cut(s) 781
Kzo9I GATC 3 cut(s) 132, 593, 927
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 2 cut(s) 352, 356
Lsp1109I GCAGC 4 cut(s) 148, 194, 308, 860
LweI GCATC 5 cut(s) 148, 394, 524, 595, 759
MaeI CTAG 2 cut(s) 828, 845
MaeII ACGT 2 cut(s) 352, 484
MaeIII GTNAC 4 cut(s) 391, 480, 531, 631
MalI GATC 3 cut(s) 134, 595, 929
MbiI CCGCTC 1 cut(s) 813
MboI GATC 3 cut(s) 132, 593, 927
MboII GAAGA 7 cut(s) 142, 221, 228, 482, 550, 563, 817
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 1 cut(s) 681
MlyI GAGTC 1 cut(s) 653
MnlI CCTC 9 cut(s) 47, 50, 78, 217, 238, 388, 433, 583, 774
Mph1103I ATGCAT 1 cut(s) 432
MseI TTAA 1 cut(s) 557
MslI CAYNNNNRTG 4 cut(s) 24, 70, 691, 743
MspA1I CMGCKG 2 cut(s) 238, 877
MspI CCGG 1 cut(s) 790
MspR9I CCNGG 3 cut(s) 398, 520, 790
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 2 cut(s) 398, 520
MwoI GCNNNNNNNGC 5 cut(s) 188, 296, 521, 778, 841
NciI CCSGG 1 cut(s) 790
NdeI CATATG 1 cut(s) 744
NdeII GATC 3 cut(s) 132, 593, 927
NheI GCTAGC 1 cut(s) 844
NlaIV GGNNCC 2 cut(s) 105, 348
NmuCI GTSAC 3 cut(s) 480, 531, 631
NsiI ATGCAT 1 cut(s) 432
NspI RCATGY 1 cut(s) 370
OliI CACNNNNGTG 2 cut(s) 70, 691
PaeI GCATGC 1 cut(s) 370
PagI TCATGA 3 cut(s) 120, 610, 859
PceI AGGCCT 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 370
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 1 cut(s) 625
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 4 cut(s) 163, 184, 298, 850
PleI GAGTC 1 cut(s) 652
PmaCI CACGTG 1 cut(s) 353
PmlI CACGTG 1 cut(s) 353
PpsI GAGTC 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 396, 518
PspCI CACGTG 1 cut(s) 353
PspGI CCWGG 2 cut(s) 396, 518
PspN4I GGNNCC 2 cut(s) 105, 348
PspPI GGNCC 2 cut(s) 85, 897
RseI CAYNNNNRTG 4 cut(s) 24, 70, 691, 743
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 1 cut(s) 557
SatI GCNGC 4 cut(s) 162, 183, 297, 849
Sau3AI GATC 3 cut(s) 132, 593, 927
Sau96I GGNCC 2 cut(s) 85, 897
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 3 cut(s) 398, 520, 790
SduI GDGCHC 1 cut(s) 349
SetI ASST 6 cut(s) 70, 355, 361, 487, 550, 766
SfaNI GCATC 5 cut(s) 148, 394, 524, 595, 759
SinI GGWCC 1 cut(s) 897
SmiI ATTTAAAT 1 cut(s) 558
SmiMI CAYNNNNRTG 4 cut(s) 24, 70, 691, 743
SphI GCATGC 1 cut(s) 370
Sse9I AATT 1 cut(s) 681
SseBI AGGCCT 1 cut(s) 443
SsiI CCGC 5 cut(s) 238, 714, 802, 813, 875
SspI AATATT 2 cut(s) 79, 893
SspMI CTAG 2 cut(s) 828, 845
StuI AGGCCT 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 396, 518, 788
StyI CCWWGG 1 cut(s) 207
SwaI ATTTAAAT 1 cut(s) 558
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 2 cut(s) 355, 487
TaqI TCGA 2 cut(s) 169, 749
TasI AATT 1 cut(s) 681
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 4 cut(s) 663, 669, 682, 698
TseFI GTSAC 3 cut(s) 480, 531, 631
TseI GCWGC 4 cut(s) 161, 182, 296, 848
Tsp45I GTSAC 3 cut(s) 480, 531, 631
TspDTI ATGAA 5 cut(s) 36, 137, 216, 293, 660
TspGWI ACGGA 3 cut(s) 60, 190, 550
TspRI CASTG 4 cut(s) 663, 669, 682, 698
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 897
XapI RAATTY 1 cut(s) 681
XceI RCATGY 1 cut(s) 370
XspI CTAG 2 cut(s) 828, 845
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.