Rh6CG153400

Protein BASIC PENTACYSTEINE4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005753
Physical Location & Seq
Reverse (-)
19945857 .. 19949929
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG153400.1

Sequence Viewer

Length: 936 bp
ATGGATGATGGTCGACAACATGAAAATGGGAGACACAAGCAGGACTATTACAGAGGGGCCCCCTCGCCGTGGAACATGACGGCTCAACAGCAAGTAAAGGAACCGAATGCCTTAGTCATGAACAAGAAGATCATGTCGATTATTGCTGAGAGGGATTCTGCAATTCGAGAACGAAATGCAGCAATCTCTGAAAAGAATGAAGCCTTTGCTGCAAGAGATGAGGCTCTCCGACAGCGCGATGAGGCATTTGCTCAGCGTGATACTGCCCTAATGGAACGAGACAATGCCTTTGCAGCCTTTCATATCCGGGATAATTCCATGAACTTCCCATTGGGTGGTGGAGTTCAACGTGGATCTAAGCGTATGCACTACCCTTCAAATCATCCAGTTAACATGGCTGATGGTCCTTACAGCACAAAAGATGTGCCTATAACTGAAGCCTTCCCAATTTCAGTATTATCCGCCGAAGCTATCAAGTCACGGCAGACAAAGCGATCAAAGGAGAATAAGGCATCCAAGGCATCCAAGGCGTCAAGAAAGAAAGTAGGTGAAGATTTGAATAGGCAGGCTTCCACTGATGGGATAAAGTACAGATCTGAGTGGGATACTCAGGATTTAGGCTTGAATCTTGTCTCATTTGACGAGACTACAATGCCTGTGCCAGTTTGCTCATGTACTGGAATGCCACGGCAGTGCTACAAATGGGGGAATGGTGGGTGGCAGTCATCTTGCTGCACTACCAATATGTCAATGTATCCACTACCCCAAATGCCAAATAAGCGCCACGCCCGGATGGGTGGGCGGAAAATGAGTGGAAGTGTTTTTACTAGATTGCTCAGTCGGCTAGCAGCAGAAGGCCATGATCTATCAGTACCACTGGATCTGAAGGAATACTGGGCCAGACATGGGACAAATCGCTACATAACAATCAAGTAG

Protein Analysis

311

Amino Acids

35.09

Weight (kDa)

9.56

Isoelectric Point (pI)

56.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAGA_bind PF06217 1 - 311 1e-115 GAGA binding protein-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 335
AccI GTMKAC 1 cut(s) 13
AccII CGCG 1 cut(s) 237
AciI CCGC 2 cut(s) 462, 802
AclWI GGATC 2 cut(s) 361, 888
AcuI CTGAAG 2 cut(s) 456, 905
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 3 cut(s) 590, 676, 873
AfiI CCNNNNNNNGG 4 cut(s) 69, 335, 579, 906
AgsI TTSAA 4 cut(s) 347, 378, 559, 625
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AleI CACNNNNGTG 1 cut(s) 691
AluBI AGCT 1 cut(s) 470
AluI AGCT 1 cut(s) 470
Alw26I GTCTC 4 cut(s) 25, 273, 637, 638
AlwI GGATC 2 cut(s) 361, 888
AoxI GGCC 3 cut(s) 57, 856, 897
ApaI GGGCCC 1 cut(s) 61
ApeKI GCWGC 5 cut(s) 179, 209, 293, 732, 848
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AspLEI GCGC 2 cut(s) 237, 783
AspS9I GGNCC 4 cut(s) 57, 58, 404, 897
AsuC2I CCSGG 2 cut(s) 308, 790
AsuHPI GGTGA 1 cut(s) 560
AsuNHI GCTAGC 1 cut(s) 844
AvaII GGWCC 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 808, 840
BbvI GCAGC 5 cut(s) 191, 196, 305, 719, 860
BccI CCATC 4 cut(s) 2, 395, 572, 787
BceAI ACGGC 4 cut(s) 52, 96, 497, 704
BciVI GTATCC 2 cut(s) 598, 765
BcnI CCSGG 2 cut(s) 308, 790
BcoDI GTCTC 4 cut(s) 25, 273, 637, 638
BfaI CTAG 2 cut(s) 828, 845
BfoI RGCGCY 1 cut(s) 784
BfuI GTATCC 2 cut(s) 598, 765
BglII AGATCT 1 cut(s) 593
BisI GCNGC 5 cut(s) 180, 210, 294, 733, 849
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 5 cut(s) 181, 211, 295, 734, 850
Bme1390I CCNGG 2 cut(s) 308, 790
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 4 cut(s) 57, 58, 404, 897
BmiI GGNNCC 4 cut(s) 58, 59, 60, 102
BmrFI CCNGG 2 cut(s) 308, 790
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 2 cut(s) 521, 530
BmtI GCTAGC 1 cut(s) 848
BmuI ACTGGG 1 cut(s) 904
Bpu1102I GCTNAGC 1 cut(s) 252
BpuMI CCSGG 2 cut(s) 308, 790
BsaHI GRCGYC 1 cut(s) 530
BsaJI CCNNGG 4 cut(s) 68, 516, 525, 686
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 335, 579, 906
Bse1I ACTGG 5 cut(s) 386, 662, 682, 882, 899
BseDI CCNNGG 4 cut(s) 68, 516, 525, 686
BseGI GGATG 5 cut(s) 10, 382, 512, 521, 798
BseLI CCNNNNNNNGG 4 cut(s) 69, 335, 579, 906
BseMII CTCAG 5 cut(s) 138, 266, 588, 623, 850
BseNI ACTGG 5 cut(s) 386, 662, 682, 882, 899
BseSI GKGCMC 1 cut(s) 61
BseXI GCAGC 5 cut(s) 191, 196, 305, 719, 860
BsgI GTGCAG 1 cut(s) 718
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 3 cut(s) 59, 858, 899
BsiSI CCGG 2 cut(s) 307, 790
BslFI GGGAC 1 cut(s) 922
BslI CCNNNNNNNGG 4 cut(s) 69, 335, 579, 906
BsmAI GTCTC 4 cut(s) 25, 273, 637, 638
BsmFI GGGAC 1 cut(s) 922
BsmI GAATGC 2 cut(s) 112, 687
BsnI GGCC 3 cut(s) 59, 858, 899
Bsp120I GGGCCC 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 6 cut(s) 129, 353, 494, 593, 862, 880
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 2 cut(s) 462, 802
BspANI GGCC 3 cut(s) 59, 858, 899
BspCNI CTCAG 5 cut(s) 139, 265, 589, 622, 849
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 4 cut(s) 58, 59, 60, 102
BspOI GCTAGC 1 cut(s) 848
BspPI GGATC 2 cut(s) 361, 888
BsrI ACTGG 5 cut(s) 386, 662, 682, 882, 899
BssECI CCNNGG 4 cut(s) 68, 516, 525, 686
BssMI GATC 6 cut(s) 129, 353, 494, 593, 862, 880
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 2 cut(s) 516, 525
BstACI GRCGYC 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 567, 846
BstDEI CTNAG 7 cut(s) 112, 147, 252, 357, 597, 609, 836
BstDSI CCRYGG 2 cut(s) 68, 686
BstF5I GGATG 5 cut(s) 10, 382, 512, 521, 798
BstFNI CGCG 1 cut(s) 237
BstH2I RGCGCY 1 cut(s) 784
BstHHI GCGC 2 cut(s) 237, 783
BstKTI GATC 6 cut(s) 132, 356, 497, 596, 865, 883
BstMAI GTCTC 4 cut(s) 25, 273, 637, 638
BstMBI GATC 6 cut(s) 129, 353, 494, 593, 862, 880
BstMWI GCNNNNNNNGC 7 cut(s) 209, 293, 490, 518, 527, 778, 841
BstSCI CCNGG 2 cut(s) 306, 788
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 5 cut(s) 191, 196, 305, 719, 860
BstX2I RGATCY 3 cut(s) 353, 593, 880
BstYI RGATCY 3 cut(s) 353, 593, 880
BsuI GTATCC 2 cut(s) 598, 765
BsuRI GGCC 3 cut(s) 59, 858, 899
BtgI CCRYGG 2 cut(s) 68, 686
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 5 cut(s) 10, 382, 512, 521, 798
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 3 cut(s) 573, 698, 875
Cac8I GCNNGC 2 cut(s) 567, 846
CciI TCATGA 1 cut(s) 117
CfoI GCGC 2 cut(s) 237, 783
Cfr13I GGNCC 4 cut(s) 57, 58, 404, 897
CseI GACGC 1 cut(s) 519
Csp6I GTAC 3 cut(s) 589, 675, 872
CviAII CATG 9 cut(s) 20, 76, 118, 133, 319, 394, 672, 860, 905
CviQI GTAC 3 cut(s) 589, 675, 872
DdeI CTNAG 7 cut(s) 112, 147, 252, 357, 597, 609, 836
DpnI GATC 6 cut(s) 131, 355, 496, 595, 864, 882
DpnII GATC 6 cut(s) 129, 353, 494, 593, 862, 880
EciI GGCGGA 2 cut(s) 451, 817
Eco130I CCWWGG 2 cut(s) 516, 525
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 404
Eco57I CTGAAG 2 cut(s) 456, 905
EcoO109I RGGNCCY 2 cut(s) 57, 58
EcoT14I CCWWGG 2 cut(s) 516, 525
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 516, 525
FaeI CATG 9 cut(s) 23, 79, 121, 136, 322, 397, 675, 863, 908
FaqI GGGAC 1 cut(s) 922
FatI CATG 9 cut(s) 19, 75, 117, 132, 318, 393, 671, 859, 904
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 5 cut(s) 180, 210, 294, 733, 849
FokI GGATG 5 cut(s) 17, 369, 499, 508, 805
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 5 cut(s) 180, 210, 294, 733, 849
FspBI CTAG 2 cut(s) 828, 845
GlaI GCGC 2 cut(s) 236, 782
GluI GCNGC 5 cut(s) 180, 210, 294, 733, 849
HaeII RGCGCY 1 cut(s) 784
HaeIII GGCC 3 cut(s) 59, 858, 899
HapII CCGG 2 cut(s) 307, 790
HgaI GACGC 1 cut(s) 519
HhaI GCGC 2 cut(s) 237, 783
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 9 cut(s) 23, 79, 121, 136, 322, 397, 675, 863, 908
Hin6I GCGC 2 cut(s) 235, 781
HinP1I GCGC 2 cut(s) 235, 781
HincII GTYRAC 2 cut(s) 14, 391
HindII GTYRAC 2 cut(s) 14, 391
HinfI GANTC 2 cut(s) 155, 625
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 307, 790
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 2 cut(s) 14, 391
Hpy188I TCNGA 4 cut(s) 190, 230, 598, 885
Hpy188III TCNNGA 4 cut(s) 118, 167, 534, 611
Hpy8I GTNNAC 2 cut(s) 14, 391
HpyAV CCTTC 4 cut(s) 384, 451, 848, 880
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 6 cut(s) 161, 179, 212, 293, 367, 735
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 293, 490, 518, 527, 778, 841
HpyF3I CTNAG 7 cut(s) 112, 147, 252, 357, 597, 609, 836
HpySE526I ACGT 1 cut(s) 349
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 9 cut(s) 23, 79, 121, 136, 322, 397, 675, 863, 908
HspAI GCGC 2 cut(s) 235, 781
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 6 cut(s) 129, 353, 494, 593, 862, 880
Lsp1109I GCAGC 5 cut(s) 191, 196, 305, 719, 860
LweI GCATC 2 cut(s) 521, 530
MaeI CTAG 2 cut(s) 828, 845
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 1 cut(s) 477
MalI GATC 6 cut(s) 131, 355, 496, 595, 864, 882
MboI GATC 6 cut(s) 129, 353, 494, 593, 862, 880
MboII GAAGA 2 cut(s) 139, 563
MflI RGATCY 3 cut(s) 353, 593, 880
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 3 cut(s) 162, 313, 447
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 5 cut(s) 47, 73, 144, 214, 235
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 2 cut(s) 24, 691
MspI CCGG 2 cut(s) 307, 790
MspR9I CCNGG 2 cut(s) 308, 790
Mva1269I GAATGC 2 cut(s) 112, 687
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 7 cut(s) 209, 293, 490, 518, 527, 778, 841
NciI CCSGG 2 cut(s) 308, 790
NdeII GATC 6 cut(s) 129, 353, 494, 593, 862, 880
NheI GCTAGC 1 cut(s) 844
NlaIII CATG 9 cut(s) 23, 79, 121, 136, 322, 397, 675, 863, 908
NlaIV GGNNCC 4 cut(s) 58, 59, 60, 102
NmuCI GTSAC 1 cut(s) 477
OliI CACNNNNGTG 1 cut(s) 691
PagI TCATGA 1 cut(s) 117
PctI GAATGC 2 cut(s) 112, 687
PfeI GAWTC 2 cut(s) 155, 625
PflMI CCANNNNNTGG 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 5 cut(s) 181, 211, 295, 734, 850
PspN4I GGNNCC 4 cut(s) 58, 59, 60, 102
PspOMI GGGCCC 1 cut(s) 57
PspPI GGNCC 4 cut(s) 57, 58, 404, 897
PsuI RGATCY 3 cut(s) 353, 593, 880
RsaI GTAC 3 cut(s) 590, 676, 873
RsaNI GTAC 3 cut(s) 589, 675, 872
RseI CAYNNNNRTG 2 cut(s) 24, 691
SalI GTCGAC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 5 cut(s) 180, 210, 294, 733, 849
Sau3AI GATC 6 cut(s) 129, 353, 494, 593, 862, 880
Sau96I GGNCC 4 cut(s) 57, 58, 404, 897
ScrFI CCNGG 2 cut(s) 308, 790
SduI GDGCHC 1 cut(s) 61
SetI ASST 3 cut(s) 352, 472, 550
SfaNI GCATC 2 cut(s) 521, 530
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 24, 691
Sse9I AATT 3 cut(s) 162, 313, 447
SsiI CCGC 2 cut(s) 462, 802
SspMI CTAG 2 cut(s) 828, 845
StyD4I CCNGG 2 cut(s) 306, 788
StyI CCWWGG 2 cut(s) 516, 525
TaiI ACGT 1 cut(s) 352
TaqI TCGA 3 cut(s) 13, 137, 166
TasI AATT 3 cut(s) 162, 313, 447
TatI WGTACW 2 cut(s) 588, 674
TfiI GAWTC 2 cut(s) 155, 625
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 3 cut(s) 580, 698, 882
TseFI GTSAC 1 cut(s) 477
TseI GCWGC 5 cut(s) 179, 209, 293, 732, 848
Tsp45I GTSAC 1 cut(s) 477
TspDTI ATGAA 5 cut(s) 36, 134, 213, 290, 335
TspRI CASTG 3 cut(s) 580, 698, 882
Van91I CCANNNNNTGG 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 13
XspI CTAG 2 cut(s) 828, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.